PSIII-11 The effect of feeding supplemental zeolite (clinoptilolite) of two different particle sizes on measures of nitrogen utilization and nutrient digestibility in finishing beef heifers

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 287-287
Author(s):  
Cheyanne A Myers ◽  
Mario de Haro Marti ◽  
Mireille Chahine ◽  
Gwinyai E Chibisa
Keyword(s):  
Particle Size ◽  
Nutrient Intake ◽  
Nutrient Digestibility ◽  
Particle Sizes ◽  
Sample Collection ◽  
Beef Heifers ◽  
Plasma Urea ◽  
Ruminal Ph ◽  
N Utilization

Abstract Clinoptilolite (CLN), could potentially improve nitrogen (N) utilization when fed to beef cattle as it can bind ruminal-ammonia-N (NH3-N), limiting its loss and subsequent detoxification into urea-N, which is released into blood and is excreted in urine. However, the effectiveness of CLN is influenced by physical properties such as particle size. Although decreasing the particle size has been shown to increase the binding of ammonium in-vitro, this remains to be evaluated in vivo. Therefore, the objective of this study was to determine the effects of feeding CLN of two different particle sizes (30 and 400 µm) on ruminal NH3-N and plasma-urea-N (PUN) concentrations, ruminal pH, and nutrient intake and apparent total-tract digestibility. Six ruminally-cannulated beef heifers (mean initial BW± SD, 620.8 ± 30.15) were used in a replicated 3 × 3 Latin square design with 21 d periods (sample collection from d 15 to 21). Dietary treatments were: 1) finishing ration with no supplement (CON), 2) CON +30-µm CLN (CL-30), and 3) CON + 400-µm CLN (CL-400). Clinoptilolite was top-dressed (2.5% of diet DM) during morning feeding. Intake was measured daily. Ruminal fluid was collected on d 19 for NH3-N analysis and blood was collected 3 h post-feeding on d 21 for PUN analysis. Indwelling pH loggers were used to measure ruminal pH (d 15 to 21) and grab fecal samples were collected from d 19 to 21 to determine total-tract nutrient digestibility. Statistical analysis was conducted using PROC MIXED in SAS. There was no treatment effect (P ≥ 0.13) on ruminal NH3-N and PUN concentrations, ruminal pH, and nutrient (DM, OM, NDF, ADF and CP) intake and apparent total tract digestibility. In conclusion, feeding CLN to finishing heifers had no effect on measures of N utilization, ruminal pH and nutrient intake and apparent total-tract digestibility.

scholarly journals 292 Altering the particle size of supplemental zeolite (clinoptilolite): Effects on nitrogen utilization and nutrient digestibility in backgrounding cattle

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 213-213
Author(s):  
Caitlin Klaeui ◽  
Mario de Haro Martí ◽  
Mireille Chahine ◽  
Gwinyai E Chibisa
Keyword(s):  
Particle Size ◽  
Latin Square ◽  
Nutrient Digestibility ◽  
Neutral Detergent Fiber ◽  
Beef Heifers ◽  
Plasma Urea ◽  
Spot Urine ◽  
Post Feeding ◽  
N Utilization ◽  
D 20

Abstract The objective of this study was to evaluate the effects of feeding supplemental clinoptilolite with a particle size of either 30 microns or 400 microns (US 40 mesh) on measures of nitrogen (N) utilization and nutrient digestibility in backgrounding cattle. Six ruminally cannulated beef heifers were used in a replicated 3 × 3 Latin square design with 21-d periods. The dietary treatments were: 1) a basal forage-based backgrounding TMR with no supplement (CON), 2) CON + 30-micron clinoptilolite (CL-30), and 3) CON + 400-micron clinoptilolite (CL-400). Clinoptilolite was top-dressed (2.5% of diet DM) during morning feeding (1100 h). Ruminal fluid (d 19 at 2, 3, 4, 6 and 12 h post-feeding), spot urine and grab fecal (d 19 at 1400 and 1900; d 20 at 0300, 0700, 1500, and 2300 h; d 21 at 0500 and 1100 h), and blood samples (d 21 at 3 h post-feeding) were collected and analyzed for nutrients and metabolites. There was no diet effect (P = 0.50) on ruminal ammonia-N (NH3-N) and plasma urea-N (PUN) concentrations. Similarly, urine N and urea-N (UUN), and fecal N excretion did not differ (P ≥ 0.28) across diets. There was no diet effect (P ≥ 0.11) on dry matter (DM) and organic matter (OM) intake, and apparent total tract DM, crude protein (CP), and neutral detergent fiber (NDF) digestibility. However, although it did not differ for the CL-400 compared to CON and CL-30 heifers, apparent total tract OM digestibility was greater for CL-30 than CON heifers. In summary, although feeding supplemental clinoptilolite with a particle size of either 30- or 400-microns to beef heifers did not enhance N utilization, apparent total tract OM digestibility was greater for heifers fed supplemental 30-micron but not 400-micron clinoptilolite compared to the CON diet.

scholarly journals PSVIII-27 Interactions between the stage of maturity of Eragrostis tef hay and supplemental energy source on forage utilization in beef heifers

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 325-325
Author(s):  
Allison Stevens ◽  
Cheyanne Myers ◽  
John B Hall ◽  
Gwinyai E Chibisa
Keyword(s):  
Energy Source ◽  
Eragrostis Tef ◽  
Ruminal Fermentation ◽  
Beef Heifers ◽  
Ruminal Ph ◽  
N Utilization ◽  
Beet Pulp ◽  
Forage Utilization ◽  
Energy Supplements ◽  
Microbial N

Abstract This study evaluated the interaction between the stage of maturity of Eragrostis tef (teff) grass and supplemental energy source on ruminal fermentation characteristics and nitrogen (N) utilization. Six ruminally-fistulated beef heifers were used in a 3 × 3 split-plot design (21 d periods). The whole plot factor was stage of maturity of teff hay [early- (EH) or late-heading (LH)], and the subplot factor was supplemental energy source [no supplement (CON), rolled corn grain or beet pulp pellet (BP)] fed at 0.5% of body weight. Feed intake was measured daily. Indwelling pH loggers were used to measure ruminal pH (d 14 to 21) and ruminal fluid was collected from d 19 to 21 to determine fermentation characteristics, as were feces and urine to measure N excretion. Data was analyzed using PROC MIXED in SAS. There was no stage of maturity × supplement interaction for all measurements (P ≥ 0.17). Although dry matter intake (DMI) tended to be greater (P = 0.07) for heifers fed EH than LH hay, it did not differ (P = 0.88) across supplements. Ruminal acetate concentration tended (P = 0.098) to be greater for EH than LH heifers, whereas butyrate, isobutyrate, and isovalerate concentrations were greater (P ≤ 0.045) for BPP than CON heifers. However, the duration and area pH < 6.2 and 5.8 did not differ (P > 0.12) across treatments. Except for microbial N flow, which was greater (P < 0.01) for EH than LH heifers, there was no stage of maturity or supplement effect (P ≥ 0.14) on measures of N utilization. In summary, feeding teff hay harvested at the EH than LH stage of maturity increased DMI and microbial N supply, whereas feeding corn and BP as energy supplements to beef heifers had no effect on ruminal pH.

Experimental Investigation on the Lipid-Lowering Activity of Three Novel Antilipidemic Materials In Vitro

2011 ◽  
Vol 399-401 ◽  
pp. 1568-1572 ◽  
Author(s):  
Si Rong Tan ◽  
Si Hui Wu ◽  
Bing Gao ◽  
Jun Rui Xiao ◽  
Zi Yue Guo ◽  
...  
Keyword(s):  
Particle Size ◽  
Physical Adsorption ◽  
Food Additives ◽  
Chitosan Nanoparticles ◽  
Lipid Lowering ◽  
Particle Sizes ◽  
Particle Size Analyzer ◽  
Determinant Factor ◽  
Lowering Activity

Chitosan is a new kind of material in life-science, it can be used in lipid-lowering. Chitosan microspheres (CTS-MP) were prepared by spray-drying, chitosan nanoparticles (CTS-NP) and chitosan-capsaicin microspheres (CTS-CAP-MP) were prepared by ionic gelation process. The effect of these samples on lowering lipids was evaluated by measuring its’ binding capacities with lipids under the conditions mimicking the gastrointestinal tract in vitro. Also, the particle sizes of chitosan preparations were examined by scanning electron microscopy (SEM) and particle size analyzer. The results showed that the lipids-binding capacities of CTS-MP and CTS-NP were far more higher than that of CTS and CTS-CAP-MP. In conclusion, CTS-MP and CTS-NP will be better fat-lowing food additives to replace chitosan. The mechanism of lipids-binding in vitro is not only physical adsorption. Particle size is not the determinant factor, electrostatic action is maybe another factor of lipids-binding.

scholarly journals Particle size and fraction of wheat bran influence short-chain fatty acid productionin vitro

2009 ◽  
Vol 102 (10) ◽  
pp. 1404-1407 ◽  
Author(s):  
Maria L. Stewart ◽  
Joanne L. Slavin
Keyword(s):  
Particle Size ◽  
Wheat Bran ◽  
Small Particle ◽  
Large Particle ◽  
Short Chain Fatty Acid ◽  
Whole Grains ◽  
Particle Sizes ◽  
Fine Particle Size

Whole grains are associated with decreased risk of chronic disease and decreased risk of obesity. Several mechanisms may be involved including SCFA production via fibre fermentation in the colon. The aim of the present study was to evaluate the role of wheat bran particle size (large/coarsev.small/fine) and wheat bran fraction (whole branv.aleuronev.aleurone by-product) in SCFA production using a batchin vitrofermentation system with human faecal inoculum. Five samples were compared: large-particle bran, small-particle bran, aleurone, coarse by-product, fine by-product. Fine by-product produced the greatest SCFA concentrations. By-product (both coarse and fine) produced greater SCFA concentrations than bran (both large and small particle sizes). Aleurone produced SCFA concentrations similar to small-particle bran. The molar percentage of butyrate at 24 h was significantly greater for large-particle bran than the other samples. Small/fine particle size and by-product fraction of bran increased SCFA production compared with large/coarse particle size, and aleurone and whole bran. Bran characteristics and composition should be considered when manufacturing foods due to the diversity of physiological effects.

scholarly journals Nutritional value, total dry matter losses, effluent production and pollutant potential in Brachiaria brizantha cv. Paiaguás grass

2021 ◽  
Vol 73 (3) ◽  
pp. 675-683
Author(s):  
J.M. Saute ◽  
T.T. Tres ◽  
M.P. Osmari ◽  
S.L. Silva ◽  
J.L.P. Daniel ◽  
...  
Keyword(s):  
Particle Size ◽  
Dry Matter ◽  
Nutritional Value ◽  
Oxygen Demand ◽  
In Vitro Digestibility ◽  
Particle Sizes ◽  
Brachiaria Brizantha ◽  
Aerobic Stability ◽  
Compaction Density

ABSTRACT The objective of the present study was to evaluate losses, production and polluting potential of the effluent, nutritional value and aerobic stability of silages of Brachiaria brizantha cv. Paiaguás grass, in different particle sizes and compaction density in silage. Three theoretical particle sizes (TTP 5; 8 and 12mm) and three compaction densities (DC 550; 600 and 650kg/m3) were evaluated, distributed in a factorial design (3 x 3), with four repetitions. The highest volume of effluent was found in silages with higher compaction densities (600 and 650kg/m3) and lower TTP (5 and 8mm). The highest chemical oxygen demand and biochemical oxygen demand were registered in the treatment with TTP of 5mm and higher DC (600 and 650kg/m3). Greater in vitro digestibility of DM was verified in the silage chopped at 5 and 8mm. There was no break in aerobic stability for 216 hours. Silage with a low compaction density 550kg/m3 and processing with a theoretical particle size of 12mm reduces effluent losses. In general, the nutritional value of Paiaguás grass was not influenced by the treatments. Different particle sizes and compaction density did not change the aerobic stability of silages.

scholarly journals Apparent total tract digestibility, ruminal fermentation, and blood metabolites in beef steers fed green-chopped cool-season forages

2020 ◽  
Vol 98 (6) ◽  
Author(s):  
Tessa M Schulmeister ◽  
Martin Ruiz-Moreno ◽  
Mariana E Garcia-Ascolani ◽  
Francine M Ciriaco ◽  
Darren D Henry ◽  
...  
Keyword(s):  
Lolium Multiflorum ◽  
Nutrient Digestibility ◽  
Neutral Detergent Fiber ◽  
Blood Metabolites ◽  
Beef Steers ◽  
Annual Ryegrass ◽  
Ruminal Fermentation ◽  
Cool Season ◽  
Plasma Urea ◽  
Ruminal Ph

Abstract An experiment was conducted during the winter of two consecutive years to evaluate the effects of feeding green-chopped cool-season forages on digestibility, ruminal fermentation, and blood parameters in beef steers. Nine ruminally cannulated Angus crossbred steers (year 1: 359 ± 79 kg; year 2: 481 ± 105 kg) received ad libitum green-chopped forages from pastures planted with one of the following mixtures: 1) OAT = Horizon 201 oats (Avena sativa L.)/Prine annual ryegrass (Lolium multiflorum Lam.) at 95 and 17 kg/ha, respectively; 2) RYE = FL401 cereal rye (Secale cereale L.)/Prine annual ryegrass (Lolium multiflorum Lam.) at 78 and 17 kg/ha, respectively; or 3) TRIT = Trical 342 triticale (X Triticosecale spp.)/Prine annual ryegrass (Lolium multiflorum Lam.) at 95 and 17 kg/ha, respectively. Intake was measured using the GrowSafe system and orts were discarded prior to subsequent feeding. After a 14-d adaptation, feed and fecal samples were collected twice daily for 4 d to determine apparent total tract nutrient digestibility using indigestible neutral detergent fiber (NDF) as an internal marker. On day 19, blood and ruminal fluid samples were collected every 3 h during a 24-h period to analyze plasma urea nitrogen (PUN) and glucose, ruminal pH, and concentration of ruminal ammonia nitrogen (NH3-N) and volatile fatty acids (VFA). Data were analyzed as a generalized randomized block design with repeated measures using the PROC MIX of SAS. No effect of treatment (P > 0.05) was observed for intake of dry matter, organic matter (OM), crude protein, NDF, or acid detergent fiber. Apparent total tract digestibility of nutrients was greater (P < 0.05) for OAT and TRIT when compared with RYE, with OM digestibility being 82.7%, 79.6%, and 69.5%, respectively. An effect of time (P < 0.01) was observed for ruminal pH. Plasma concentration of glucose was greater (P < 0.01) in steers consuming OAT, whereas steers fed RYE had greater (P < 0.05) concentrations of ruminal NH3-N and PUN, and the least concentration of total ruminal VFA (P < 0.05), despite having the greatest (P > 0.05) molar proportion of acetate, branched-chain VFA, and acetate:propionate. Increased nutrient digestibility and favorable ruminal fermentation and blood metabolites of OAT and TRIT are potentially conducive to enhanced growth performance when compared with RYE.

Effects of particle size on structural, physicochemical, and functional properties of potato residue from starch isolation and quality characteristics of residue-based starch noodles

2020 ◽  
pp. 108201322095460
Author(s):  
Md. Hasan Waliullah ◽  
Taihua Mu ◽  
Mengmei Ma ◽  
Jingwang Chen
Keyword(s):  
Particle Size ◽  
Adsorption Capacity ◽  
Functional Properties ◽  
Ultrafine Particles ◽  
Water Solubility ◽  
In Vitro Digestibility ◽  
Particle Sizes ◽  
Water Holding ◽  
Physicochemical And Functional Properties

The structural, physicochemical, and functional properties of potato residue with different mesh sizes (<180 µm, <150 µm, <106 µm, <75 µm, ultrafine) obtained from starch isolation were investigated, and its effects on the shear strength, tensile strength, cooking time, and in vitro digestibility of 5% potato residue-based starch noodles were further compared. The results showed that the protein, ash, dietary fiber, water holding capacity, and water solubility of fractionated potato residue were decreased with decreasing particle size, while the starch, fat, polyphenols, and α-amylase activity inhibition ratio were increased. Ultrafine particles exhibited the highest phenolic content (2.26 mg chlorogenic acid equivalent/g), glucose adsorption capacity (7.03 mmol/g), cholesterol adsorption capacity (16.54%), and better performance on starch noodles formulation. However, oil holding capacity and microstructure did not show any significant differences. Therefore, potato residues with desired particle sizes could be successfully used to develop fiber-enriched food products for reducing food waste.

scholarly journals Effect of thyme essential oil on rumen parameters, nutrient digestibility, and nitrogen balance in wethers fed high concentrate diets

2020 ◽  
Vol 72 (2) ◽  
pp. 573-580 ◽  
Author(s):  
A.D.B. Ribeiro ◽  
M.V.C. Ferraz Junior ◽  
D.M. Polizel ◽  
A.A. Miszura ◽  
J.P.R. Barroso ◽  
...  
Keyword(s):  
Nitrogen Balance ◽  
Nutrient Intake ◽  
Dry Matter ◽  
Nitrogen Retention ◽  
Short Chain Fatty Acids ◽  
Nutrient Digestibility ◽  
Feed Additive ◽  
Apparent Digestibility ◽  
Ruminal Ph ◽  
Feedlot Lambs

ABSTRACT This trial aimed to evaluate the effects of thyme essential oils (EO) on rumen parameters, nutrient digestibility and nitrogen balance in wethers fed with high-concentrate diet. Twenty rumen-cannulated wethers were blocked according to body weight (BW= 64.0±2.1kg), and received one of the following treatments: 25mg of monensin/kg of dry matter (DM; MON) or doses of thyme EO (1.25, 2.50 or 3.75g/kg of DM). The diet was composed of 90% concentrate. Thyme EO was composed mainly by thymol (46.6% of DM) and p-cymene (38.9% of DM). The nutrient intake and apparent digestibility were similar among treatments. The inclusion of 3.75g of thyme EO tended (P= 0.07) to increase butyrate compared to MON and 1.25OE and wethers fed with 1.25g of thyme EO tended (P= 0.07) to decrease ruminal pH on the 14th day compared to MON. The treatments did not affect acetate:propionate ratio, total short chain fatty acids (SCFA) and nitrogen retention. Results from this study suggest that adding thyme EO to high-concentrate diets may be used as an alternative to monensin as feed additive in feedlot lambs.

Effects of fibre level and particle size on rumen microbial fermentation and protein metabolism using liquid and solid associated bacteria

2001 ◽  
Vol 2001 ◽  
pp. 149-149
Author(s):  
M. Rodríguez ◽  
S. Calsamiglia ◽  
A. Ferret
Keyword(s):  
Particle Size ◽  
Microbial Fermentation ◽  
Ruminal Fermentation ◽  
Associated Bacteria ◽  
Dietary Nitrogen ◽  
Ruminal Ph ◽  
Fermentation Profile ◽  
N Metabolism

The effects of fibre level (F) and forage particle size (S) on ruminal fermentation profile is often mediated through changes in feed intake, rates of digestion or passage, ruminal pH and/or bacteria population. Therefore, most in vivo studies have confounded the direct effect of F or S with changes in the rumen environment. In vitro systems allow to control several fermentation conditions independently (pH, flow rates, intake). Total, bacterial and dietary nitrogen (N) flows are generally calculated using liquid associated bacteria (LAB), although solid associated bacteria (SAB) represent about 80% of total bacterial population in the rumen (Olobobokun and Craig, 1988). The objective of this experiment was to study the effects of F and S on microbial fermentation and N metabolism using LAB or SAB values in a dual flow continuos culture system.

scholarly journals PSXII-23 Effects of a recombinant fibrolytic enzyme on fiber digestion, ruminal fermentation, nitrogen balance and total tract digestibility of heifers fed a high forage diet

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 419-420
Author(s):  
Tao Ran ◽  
Atef Saleem ◽  
YiZhao Shen ◽  
Gabriel Ribeiro ◽  
Adrian Tsang ◽  
...  
Keyword(s):  
Nitrogen Balance ◽  
Fixed Effects ◽  
Barley Straw ◽  
Ruminal Fermentation ◽  
Sample Collection ◽  
Beef Heifers ◽  
Endoglucanase Activity ◽  
Fiber Digestion ◽  
Ruminal Ph ◽  
High Forage

Abstract The objective of this study was to investigate the effects of a recombinant fibrolytic enzyme (RFE; xylanase XYL10C) on ruminal pH and fermentation characteristics, total tract digestibility and nitrogen balance in beef heifers. The experiment was a cross-over design with two treatments and two periods, using eight ruminal cannulated beef heifers. The treatments were a basal diet containing 60% barley silage, 30% barley straw and 10% supplement (DM basis) without (control) or with RFE. The RFE XYL10C was selected specifically for ruminants using a high throughput in vitro micro assay and was sprayed onto the barley straw at a rate of 6.6 × 104 IU/kg DM 24 h before feeding. The diet was prepared daily using a data ranger. Each period was 21 d, with a 14-d adaptation and 7-d sample collection period. The digestibility and nitrogen balance were measured by total fecal and urine collection. Data were analyzed using the PROC MIXED procedure of SAS with fixed effects of treatment, and random effects of period and heifer. Feed intake (8.9 kg/d) and total tract digestibility of DM (65.5%), NDF (57.2%) and ADF (51.2%) were not affected by RFE. Ruminal pH including mean (6.52), minimum (5.79), maximum (7.05), and duration of pH &lt; 5.8 (0.64, h/d) did not differ between treatments. No treatment effects were observed for total VFA concentration (106 mM), molar proportion of acetate (68.2%) and propionate (17.5%) and acetate to propionate ratio (3.94). However, adding RFE vs. control tended (P &lt; 0.08) to increase ruminal NH3-N concentration (5.75 vs. 5.23 mM) and endoglucanase activity (44.3 vs. 40.7 µmol glucose/min/mL wet rumen contents). There were no differences in nitrogen utilization and rumen microbial protein synthesis (90.7 g/d) between treatments. The results indicate that RFE XYL10C did not improve fiber digestion in heifers fed a high forage diet.

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