PSIX-22 Assessment of the influence of IGF2 gene polymorphism in boars on economically significant traits

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 499-499
Author(s):  
Tatiana V Karpushkina ◽  
Nadezhda A Svegentseva ◽  
Margaret S Fornara ◽  
Nikolay V Bardukov ◽  
Olga V Kostyunina
Keyword(s):  
Average Daily Gain ◽  
Animal Husbandry ◽  
Productivity Index ◽  
Large White ◽  
Igf2 Gene ◽  
Productivity Data ◽  
Federal Research ◽  
Factor Effect ◽  
Resource Collection ◽  
Factor Interaction

Abstract Studies of polymorphism in the IGF2 gene are of interest due to their association with economically useful traits in pigs. The aim of this work was to study the effect of IGF2 polymorphism on the variability of meat and fattening traits in pigs and to control the preservation of high reproductive qualities. The genotyping was performed using RT-PCR approaches were generated on the genetic resource collection of the L.K. Ernst Federal Research Center for Animal Husbandry. The total of 272 Large White (LW) and 301 Landrace (L) boars was investigated. Genotypes have been identified: at LW AA 73.5%, AG 23.2%, GG 3.3%; for L AA 10.3%, AG 35.2%, GG 54.5%. Reproductive performance evaluated on 22082 farrows from these boars. Analysis of productivity data was performed taking advantage of the model: y=μ+Breed+IGF2+Breed×IGF2+e, where y is the productivity index (for traits: weight at start of the test (BWs), weight at the end of the test (BWe), age of reaching 100 kg (AGE100), average daily gain (ADG), fat thickness (BF), litter size (LS), live-born (LB) and stillborn piglets (SB), the number of weaned piglets (Wean), the weaning weight (WWT), µ is the total average for a sample of n animals; Breed - the influence of the breed factor; IGF2 - gene factor effect; Breed×IGF2 - factor interaction effect; e - error. A significant effect of the IGF2 gene on variability was revealed at P < 0.001 BWe, ADG, LS, LB, Wean, WWT, at P < 0.01 on BWs, AGE100; when factors interact at P < 0.001 on ADG, AGE100, Wean, WWT, at P < 0.05 on LB. Revealed animals with genotype AA showed the best results in terms of meat and feeding qualities, as well as reproductive qualities. This research was supported by the Ministry of science and higher education No 0445-2021-0008.

THE USE OF BIFID-CONTAINING FEED PROBIOTIC ADDITIVE IN THE FEEDING OF YOUNG CATTLE

2021 ◽  
pp. 3-3
Author(s):  
Р.В. НЕКРАСОВ ◽  
М.Г. ЧАБАЕВ ◽  
Е.Ю. ЦИС ◽  
Б.А. КАРЕТКИН ◽  
Е.А. ТЕРЕШКОВА ◽  
...  
Keyword(s):  
Average Daily Gain ◽  
Live Weight ◽  
Animal Husbandry ◽  
Bifidobacterium Bifidum ◽  
Feed Additive ◽  
Control Group ◽  
Federal Research ◽  
Newborn Calves ◽  
Young Cattle ◽  
The Moment

Для уточнения эффективности скармливания бифидосодержащей кормовой пробиотической добавки молодняку крупного рогатого скота был организован научно-хозяйственный эксперимент в опытно-экспериментальном хозяй- стве Московского региона и в научных подразделениях Федерального государственного бюджетного научного уч- реждения. Новорожденные телята были распределены на 2 группы по 13 голов с учетом живой массы. Изучаемая пробиотическая бифидосодержащая кормовая добавка включала в себя лиофилизированную микробную массу жи- вых антагонистически активных бактерий вида Bifidobacterium bifidum (не менее 1×109 КОЕ/мл бифидобактерий). Скармливание добавки осуществлялось в количестве 10 мл на 1 голову в сутки молодняку опытной группы и спо- собствовало увеличению среднесуточных приростов живой массы на 14,9% по отношению к контрольной группе. Установлено, что с момента рождения до 3-месячного возраста ее использование способствовало 100% сохранности телят, тогда как в контроле этот показатель составил 92,3%. Включение в рацион телят молочного периода про- биотика обеспечило оптимальный гомеостатический и гематологический фон при улучшении морфологических и биохимических показателей крови. Показатели иммунитета, в том числе лизоцимная и бактерицидная активности были выше по отношению к контролю на 11,8 и 4,23% соответственно. Включение в рацион телят бифидодобавки обеспечивало рост лакто- и бифидобактерий в кишечнике животных, соответственно на 10,9 и 8,5%, на фоне по- давления нежелательной микрофлоры. Таким образом, скармливание с первых дней жизни бифидосодержащей добавки телятам способствует повышению у них защитных свойств, профилактирует заболеваемость, что ведет к улучшению зоотехнических показателей производства. To clarify the effectiveness of feeding probiotic bifid-containing feed probiotic additives to young cattle, a scientific and economic experiment was organized in the experimental farm of the Moscow region, and in the scientific divisions of the L.K. Ernst Federal Research Center for Animal Husbandry. Newborn calves were divided into 2 groups of 13 heads, taking into account the live weight. The probiotic bifid-containing feed additive under study contained a lyophilized microbial mass of live antagonistically active bacteria of the Bifidobacterium bifidum species (at least 1×109 CFU / ml of bifidobacteria). Feeding of the supplement was carried out in an amount of 10 ml per 1 head per day to young animals of the experimental group and contributed to an increase in the average daily gain of live weight by 14.9% compared to the control group. It was found that from the moment of birth to 3 months of age, the use contributed to 100% of the safety of calves, while in the control this indicator was 92.3%. The inclusion of probiotic in the diet of calves of the dairy period provided an optimal homeostatic and hematological background while improving the morphological and biochemical parameters of the blood. The indicators of immunity, including lysozyme and bactericidal activity, were higher in relation to the control by 11.8 and 4.23%, respectively. The inclusion of bifid supplements in the calves ' diet ensured the growth of lacto-and bifidobacteria in the intestines of animals, respectively, by 10.9 and 8.5%, against the background of suppression of undesirable microflora. Thus, feeding bifid-containing additives to calves from the first days of life increases their protective properties, prevents morbidity, which leads to an improvement in zootechnical production indicators.

scholarly journals Drone brood fed supplement impacts on the folliculogenesis in growing gilts

2020 ◽  
Vol 90 (6) ◽  
pp. 583-592
Author(s):  
Elena Kistanova ◽  
◽  
Elena Zdoroveva ◽  
Michail Nevitov ◽  
Aleksey Nosov ◽  
...  
Keyword(s):  
Growth Promotion ◽  
Average Daily Gain ◽  
Animal Husbandry ◽  
Cumulus Cells ◽  
Biological Properties ◽  
White Female ◽  
Large White ◽  
Primordial Follicles ◽  
Drone Brood ◽  
The Impact

The biological properties of bee drone brood make it an ideal additive for growth promotion in animal husbandry instead of banned hormonal anabolics and antibiotics. However, the drone brood action on mammalian ovaries has not been well studied. The present study analyzes the impact of drone brood homogenate (DBH) in the diet of growing gilts on folliculogenesis. Large White female pigs at the age of 35 days were randomly divided into two groups of 10 animals each, and fed with the same basal diets. The experimental group was supplemented with 25 mg/kg forage of DBH for 180 days, after which the animals were slaughtered and morphometric, histological and immunohistochemical evaluation of their ovaries was performed. In addition, the expression of ovarian growth factors BMP15 and GDF9 in oocytes and cumulus cells was analyzed by RT-PCR. A significant increase in body weight and average daily gain at day 145 in the DBH-supplemented group was established. The length of the ovaries in the treated animals also was enhanced. More pools of primordial follicles, involved in intensive growth, as well as a larger diameter of primary and tertiary follicles were found in the ovaries of DBH-supplemented animals. These findings corresponded with an increase in the expression of GDF9 mRNA in the oocytes and cumulus cells. At the same time, signs of atresia in the Graafian follicles of treated animals were observed. The supplementation with DBH stimulates the early stages of folliculogenesis in gilts, but provokes atresia in the last stage of follicular development.

The use of whole wheat grain in feeding of broiler chickens

2020 ◽  
pp. 3-8
Author(s):  
V. Khamitova ◽  
A. Osmanyan
Keyword(s):  
Broiler Chickens ◽  
Average Daily Gain ◽  
Live Weight ◽  
Economic Effectiveness ◽  
Metabolic Energy ◽  
Productivity Index ◽  
Feed Consumption ◽  
Meat Production ◽  
Wheat Grain ◽  
Whole Wheat

An experiment has been conducted to determine the effectiveness of growing broiler chickens when whole wheat grain has been included in the compound feed. The purpose of the research was to develop an appropriate scheme and dose for feeding whole wheat grain depending on the age of broilers, while observing the normative content of metabolic energy and nutrients in the diets, and to determine the economic effectiveness of rearing of broilers. To determine the effectiveness, data on live weight, growth rate, livability, uniformity of broiler population by live weight and variability of live weight of chickens have been studied. In the course of the work, the livability, average daily gain, feed conversion, homogeneity and variability coefficients, and the productivity index have been calculated. An increase in the live weight of broilers, as well as the rate of growth in pre-slaughter age when using whole grains in poultry diets, as well as a decrease in feed consumption per unit of live weight gain has been revealed. In conclusion, the economic effectiveness of broiler meat production has determined when whole grain has been included in diets. The use of whole wheat grain as an additive to the main diet in broiler feeding allowed to increase profits and increase the level of profitability. It has been found as a result of research that it is advisable to add whole wheat grain to the main diet for broilers at the age of 8–14 days in an amount of 5 % of the feed weight, at the age of 15–21 days – 15 %, at the age of 22–28 days – 20 %, at the age of 21–29 days – 30 % of grain without reducing the overall nutritional value of the diet.

scholarly journals The Population Structure and Selection Signal Analysis of Shenxian Pigs Based on Genome Resequencing Technology

2021 ◽  
Author(s):  
Diao Liu ◽  
Chunlian Lu ◽  
Shang Li ◽  
Mengyu Jia ◽  
Yutao Miao ◽  
...  
Keyword(s):  
Candidate Genes ◽  
Meat Quality ◽  
Genetic Relationships ◽  
Animal Husbandry ◽  
Hebei Province ◽  
Whole Genome ◽  
Genome Resequencing ◽  
Large White ◽  
Bioinformatics Analyses ◽  
Pig Breeds

Abstract Shenxian pigs are the only local black pig of Hebei Province, and were listed in the Genetics of Livestock and Poultry Resources of China in 2016. This breed of pig is considered to be a valuable local pig germplasm genetic resource in China. When compared with other introduced pig breeds, the Shenxian pig breed is characterized with early sexual maturity, short oestrus intervals, large litter sizes, and good meat quality, which are all of good research significance. However, the Shenxian pig variety was previously declared extinct in 2004 due to the introduction of a large number of foreign pig breeds. In order to preserve and study the Shenxian pig breed, the Hebei Zhengnong Animal Husbandry Co., Ltd. was established in Hebei Province for the purpose of preserving the purified Shenxian pig strain. In the present study, in order to understand the genetic variations of Shenxian pigs, identify selected regions related to superior traits, and accelerate the breeding processes of Shenxian pigs, the whole genome of the Shenxian pigs was resequenced and compared with that of large white pigs. The goal was to explore the germplasm characteristics of Shenxian pigs.The results obtained in this research investigation revealed that the genetic relationships of the Shenxian pig breed were complex, and that sub-populations could be identified within the general population. A total of 23M SNP sites were obtained by whole genome resequencing, and 1,509 selected sites were obtained via bioinformatics analyses. It was determined after annotation that a total of 19 genes were enriched in three items of bioengineering, molecular function, and cell composition.During this research investigation, the aforementioned 19 genes were subjected to GO and KEGG analyses. Subsequently, the candidate genes related to cell proliferation were obtained (DMTF1 and WDR5), which were considered to possibly be related to the slow growth and development of Shenxian pigs. In addition, the candidate genes related to lactation were obtained (CSN2 and CSN3). However, no genes related to meat quality traits were successfully screened.

scholarly journals The pattern of genetic diversity of different breeds of pigs based on microsatellite analysis

2020 ◽  
Vol 24 (7) ◽  
pp. 747-754
Author(s):  
V. R. Kharzinova ◽  
N. A. Zinovieva
Keyword(s):  
Genetic Diversity ◽  
Genetic Relationships ◽  
Molecular Genetic ◽  
Genetic Material ◽  
Animal Husbandry ◽  
Molecular Genetic Analysis ◽  
Fixation Index ◽  
Large White ◽  
Black And White ◽  
The Republic

One of the main tasks of genetics and animal breeding is the assessment of genetic diversity and the study of genetic relationships between different breeds and populations using molecular genetic analysis methods. We analysed the polymorphism of microsatellites and the information on the state of genetic diversity and the population structure of local breeds in Russia: the Kemerovo, the Berkshire, the Liven, the Mangalitsa, and the Civilian; in the Republic of Belarus: the Large White and the Black-and-White; and in Ukraine: the White Steppe, as well as commercial breeds of imported origin of domestic reproduction: the Large White, the Landrace, and the Duroc. The materials used for this study were the tissue and DNA samples extracted from 1,194 pigs and DNA of the UNU “Genetic material bank of domestic and wild animal species and birds” of the L.K. Ernst Federal Research Center for Animal Husbandry. Polymorphisms of 10 microsatellites (S0155, S0355, S0386, SW24, SO005, SW72, SW951, S0101, SW240, and SW857) were determined according to the previously developed technique using DNA analyser ABI3130xl. To estimate the allele pool of each population, the average number of alleles (NA), the effective number of alleles (NE ) based on the locus, the rarified allelic richness (AR), the observed (HO ) and expected (HE ) heterozygosity, and the fixation index (FIS) were calculated. The degree of genetic differentiation of the breeds was assessed based on the pairwise values of FST and D. The analysis of the allelic and genetic diversity parameters of the local breeds showed that the maximum and minimum levels of polymorphism were observed in pigs of the Ukrainian White Steppe breed (NA = 6.500, NE = 3.709, and AR = 6.020) and in pigs of the Duroc breed (NA = 4.875, NE = 2.119, and AR = 3.821), respectively. The highest level of genetic diversity was found in the Large White breed of the Republic of Belarus (HO = 0.707 and NE = 0.702). The minimum level of genetic diversity was found in pigs of the imported breeds – the Landrace (HO = 0.459, HE = 0.400) and the Duroc (HO = 0.480, HE = 0.469) – indicating a high selection pressure in these breeds. Based on the results of phylogenetic analysis, the genetic origin of Large White pigs, the breeds, from which the Berkshire pigs originated, and the genetic detachment of the Landrace from the Mangalitsa breeds were revealed. The cluster analysis showed a genetic consolidation of the Black-and-White, the Berkshire, and the Mangalitsa pigs. Additionally, the imported breeds with clustering depending on the origin were characterised by a genetic structure different from that of the other breeds. The information obtained from these studies can serve as a guide for the management and breeding strategies of the pig breeds studied, to allow their better use and conservation.

EFFECT OF VARIOUS FORMS OF SELENIUM ON THE PRODUCTIVITY AND METABOLISM OF FATTENED YOUNG PIGS IN PRODUCTION CONDITIONS

2021 ◽  
pp. 55-65
Author(s):  
M.G. CHABAEV ◽  
◽  
E.YU. TSIS ◽  
R.V. NEKRASOV ◽  
M.I. KLEMENTEV
Keyword(s):  
Blood Cells ◽  
Average Daily Gain ◽  
White Blood Cells ◽  
Live Weight ◽  
Growing Pigs ◽  
Control Group ◽  
Protein Activity ◽  
Large White ◽  
Organic Selenium ◽  
Young Pigs

Selenium plays an important role for maintaining complex and adequate nutrition of fattened young pigs. It also has a big biological importance to maintain growth and health, as well as for biochemical and physiological processes This study presents the results of a production experiment to determine the effect of organic and inorganic forms of selenium on growth indicators, immunohematological blood parameters and slaughter qualities of fattened pigs in period I and II. Test feeding with various forms of selenium has been implemented with two groups of analogs (N = 60; n = 30), taking into account age and live weight. The experiment was conducted on castrates of large white breed, which were divided into two groups of 30 heads each following the principle of analogues (taking into account their age, live weight). The studied fattened pigs received standard complete feeds of the SK-5 and SK-6 types for a period of 105 days. Growing pigs were fed with organic selenium for 20 mg/kg as part of the feed 64 mixture per head in the first and second period of fattening, while the animals of the control group were fed with 0.3 mg/kg of the inorganic form of selenium. Including a chelated form of selenium in the complete feed of castrates at the rate established in the course of scientific and economic studies has led to 757 g of average daily gain, which is 8.1% more as compared to the control group. The concentration of red blood cells in the blood of growing pigs fed with 20 mg/kg of selenium chelate increased by 6.3%, hemoglobin – by 2.4%, and white blood cells – by 7.1% as compared to the control. Feeding organic selenium to fattened young pigs contributed to a significant increase in specific units of protein activity by 38.65% (p < 0.01), BASC – by 7.63%, as well as lysis – by 40.06% (p < 0.05), and phagocytic activity by 3.33% in blood serum as compared to the control. The half-carcasses of hogs who had received fed rations including selenium chelate fetured slaughter weight and slaughter yield, respectively, higher – by 6.4 and 0.6% as compared with the control analogues. The use of the established norm of organic selenium in the diets of fattened young pigs according to the results of production testing is economically profitable and contributes to a per-head profit of 420 rubles.

scholarly journals 19 Effects of glutathione on growth performance and intestinal health of piglets

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 19-19
Author(s):  
Xianyong Ma ◽  
Zhimei Tian ◽  
Yiyan Cui ◽  
Zhichang Liu ◽  
Huijie Lu
Keyword(s):  
Growth Performance ◽  
Average Daily Gain ◽  
Control Group ◽  
Standard Diet ◽  
Intestinal Health ◽  
Large White ◽  
Crypt Depth ◽  
Mda Content ◽  
Villous Height ◽  
Mucosal Morphology

Abstract Object: The aim of this experiment was to study the effects of different levels of glutathione (GSH) on growth performance and intestinal health of piglets. Methods: A total of 180 piglets of 21-day-old (Duroc × Landrace × Large White) were randomly divided into 5 groups. The control group was fed a standard diet based on NRC 2012. The treatment group 1 - 4 was supplemented with 50, 100, 200 and 400mg/kg GSH. The experiment lasted 28 days. Results: Compared with the control group, 50mg/kg GSH significantly increased the piglet’s skin and gross color, average daily gain (ADG) and decreased feed gain ratio (F/G) (P &lt; 0.05), while the effect of 100, 200, 400 mg/kg GSH was contrary. The supplementation of 50mg/kg GSH significantly increased the mucosal villi height, the ratio of villous height to crypt depth (VH/CD) and decreased the crypt depth (P &lt; 0.05) in duodenum, jejunum and ileum (P &lt; 0.05), while the effect of 100, 200, 400 mg/kg GSH was contrary; The supplementation of 50mg/kg GSH decreased the concentration of triglyceride in plasma (P &lt; 0.05); The supplementation of 50, 100, 200, 400mg/kg GSH had no effect on the antioxidative indexes of blood, but 400mg/kg GSH increased the activities of T-AOC, GSH, SOD and decreased MDA content in jejunum and ileum; 400mg/kg GSH increased the content of T-SOD in liver (P &lt; 0.05), while 50mg/kg GSH had no effect on SOD, GSH-PX, GSH-SOD in intestine and liver (P &gt; 0.05). 50mg/kg GSH significantly increased T-AOC activity and decreased MDA content in muscle (P &lt; 0.05). Conclusion: Supplementation of 50mg/kg GSH in piglets` diet was the most appropriate and it could significantly improve skin and gross color, growth performance and intestinal mucosal morphology, it also increased the antioxidation ability in muscle of piglets.

Growth performance and carcass traits of Large White, Mukota and Large White ✕ Mukota F1crosses given graded levels of maize cob meal

2004 ◽  
Vol 78 (1) ◽  
pp. 61-66 ◽  
Author(s):  
A. T. Kanengoni ◽  
K. Dzama ◽  
M. Chimonyo ◽  
J. Kusina ◽  
S. M. Maswaure
Keyword(s):  
Crude Protein ◽  
Average Daily Gain ◽  
Metabolizable Energy ◽  
Backfat Thickness ◽  
Food Conversion ◽  
Large White ◽  
Daily Food ◽  
Maize Cob ◽  
Rate Of Decline ◽  
Daily Gain

AbstractA study was conducted to compare the performance of Mukota, Large White (LW) and LW ✕ Mukota F1pigs given increasing levels of maize cob meal. Sixteen female weaners of each genotype were given, ad libitum, diets containing 0, 100, 200 and 300 g maize cob meal per kg of diet for 14 weeks. The diets were designed to contain similar levels of protein (ca. 160 g crude protein per kg) and energy (ca. 9 MJ metabolizable energy per kg). Average daily food intake per unit metabolic body weight (ADFI per kg M0·75), average daily gain (ADG) and food conversion ratio (FCR) were determined. The pigs were slaughtered and cold dressed mass (CDM) and backfat thickness were determined for each pig. The ADFI per kg M0·75was similar among the four diets for the three genotypes. The rate of decline in ADG was higher (P < 0·05) in the LW than in the other two genotypes. The FCR in the Mukota was poorer (P < 0·05) than that in the LW and the F1crosses. The Mukota had the highest (P < 0·05) backfat thickness (K5 and K7·5) values of the three genotypes across the four diets, followed by the F1crosses. The CDM values for the LW and the F1crosses were similar and were higher (P < 0·05) than those for the Mukota. The findings indicate that F1crosses and the Mukota were better able to utilize diets containing high levels of maize cob meal than LW pigs.

The effect of genotype and castration method on the eating quality characteristics of pork from male pigs

2003 ◽  
Vol 77 (1) ◽  
pp. 67-72 ◽  
Author(s):  
D. N. D’Souza ◽  
B. P. Mullan
Keyword(s):  
Average Daily Gain ◽  
Subcutaneous Fat ◽  
Quality Characteristics ◽  
Taste Panel ◽  
Eating Quality ◽  
Large White ◽  
Overall Acceptability ◽  
Daily Gain ◽  
Genotype A

AbstractSixty crossbred (Large White × Landrace × Duroc) pigs were used to compare the growth performance, carcass and pork quality characteristics of entire, surgically castrated and immunologically castrated male pigs from two Western Australian commercial genotypes (genotype A : ‘lean’ genotype and genotype B : propensity for increased subcutaneous fat deposition). Pigs from genotype B had a higher average daily gain and a superior food conversion ratio compared with pigs from genotype A. Entire males had lower backfat compared with surgically and immunologically castrated male pigs. Pork from entire males was drier, tougher and had lower overall acceptability scores compared with surgically or immunologically castrated male pigs. Pork from genotype A pigs had a lower m. longissimus thoracis (LT) ultimate pH and higher drip loss compared with pork from genotype B pigs. However, consumer taste panel assessment indicated that pork from genotype A pigs was more tender, had higher juiciness and overall acceptability scores compared with pork from genotype B pigs. Within genotype A, the consumer taste panel preferred pork from surgically castrated pigs compared with either entire or immunologically castrated pigs. Within genotype B, the consumer taste panel preferred pork from immunologically castrated pigs compared with either entire or surgically castrated male pigs. These results indicate that interactions between genotype and castration method can significantly influence eating quality of pork.

scholarly journals The visfatin (NAMPT; PBEF1) gene polymorphisms and associations with meat performance traits in three pig breeds kept in the Czech Republic

2009 ◽  
Vol 54 (No. 10) ◽  
pp. 443-447 ◽  
Author(s):  
J. Zrůstová ◽  
A. Knoll ◽  
T. Urban ◽  
S. Čepica
Keyword(s):  
Average Daily Gain ◽  
Backfat Thickness ◽  
Large White ◽  
Nicotinamide Phosphoribosyltransferase ◽  
The Czech Republic ◽  
Lean Meat ◽  
Meat Content ◽  
The Difference ◽  
Daily Gain

Visfatin encoded by the <I>NAMPT</I> gene <I>(nicotinamide phosphoribosyltransferase,</I> formerly also known as <I>PBEF – pre-B cell colony-enhancing factor</I>) is suggested to play a role in lipid metabolism and pathophysiology of diabetes mellitus type 2. A new microsatellite <I>SCZ004</I> was detected within intron 9 of the <I>NAMPT</I> gene. In Czech Large White (<I>n</I> = 95) frequencies of alleles <I>282, 286, 287, 299</I>, and <I>304</I> were 0.02, 0.39, 0.07, 0.04, and 0.48, respectively. Allele <I>286</I> was predominant also in Landrace (<I>n</I> = 11) and Black Pied Prestice (<I>n</I> = 11) breeds. Association analysis was carried out between previously reported SNP AM999341:g.669T>C in intron 9 of the <I>NAMPT</I> gene and backfat thickness, average daily gain and lean meat content in Czech Large White (<I>n</I> = 215), Black Pied Prestice (<I>n</I> = 96) and Landrace (<I>n</I> = 105). The <I>CC</I> genotype was associated with higher backfat thickness (<I ≤ 0.01) in Black Pied Prestice, however in Czech Large White <I>CC</I> was associated with lower backfat thickness when compared to <I>TT</I> and <I>CT</I> genotypes (<I ≤ 0.05). In Czech Large White, <I>CC</I> genotype was associated with higher lean meat content when compared to <I>CT</I> (not significant) and <I>TT</I> genotype (<I ≤ 0.05) while in Landrace <I>CC</I> had the lowest lean meat content when compared to <I>CT</I> and <I>TT</I> genotypes but only the difference between <I>CC</I> and <I>CT</I>T was statistically significant (<I ≤ 0.05). In Black Pied Prestice no association with lean meat content was found. Average daily gain was not associated with the SNP in any breed.

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