scholarly journals PSVII-10 Xylanase improved the nutrient and energy digestibility of diets high in insoluble corn fiber fed to swine following a 36-d dietary adaptation period

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 216-217
Author(s):  
Amy L Petry ◽  
Nichole F Huntley ◽  
Mike R Bedford ◽  
John F Patience

Abstract The efficacy of xylanase in practical swine diets is inconsistent and poorly understood. The experimental objective was to investigate the efficacy of xylanase in growing pigs fed a diet high in insoluble corn fiber and afforded a longer adaptation period than typically reported in the literature. Sixty gilts (25.43 ± 0.88 kg BW; L337 X Camborough, PIC, Hendersonville, TN; n = 15 per treatment), were blocked by weight, housed individually, and randomly assigned to one of four dietary treatments: a low-fiber control (LF; 8.45% NDF), a 30% corn bran high-fiber control (HF; 24.5% NDF), HF + 100 mg/kg of xylanase (HF+XY; Econase XT 25P; AB Vista, Marlborough, UK), and HF + 50 mg/kg of arabinoxylan-oligosaccharide (HF+AX; 3-7 degrees of polymerization). Diets contained 0.5% chromium (III) oxide. Gilts were fed ad libitum for 36 d, followed by a 7-d period of adaptation to limit feeding (80% of average ad libitum intake) and housing in metabolism crates, followed by 3 d of urine and fecal collection. Data were analyzed using PROC MIXED (SAS; 9.4) as a randomized complete block design with pig as the experimental unit, block and replicate as random effects, and treatment as a fixed effect. Compared to the LF diet, the HF diet reduced the apparent total tract digestibility (ATTD) of DM (88.7 vs. 75.6%), GE (89.0 vs. 77.4%), CP (87.2 vs. 81.8%), NDF (63.3 vs. 44.4%), ADF, and hemicellulose (P = 0.01). Xylanase supplementation, but not arabinoxylan-oligosaccharide, when compared to the HF control diet, improved the ATTD of DM by 3.3% (78.2 vs. 75.6%), GE by 2.2% (79.1 vs. 77.4%), CP by 2.9% (84.2 vs. 81.8%), NDF by 17.5% (53.9 vs. 44.4%), ADF by 16.3% (52.1 vs. 42.9%), and hemicellulose by 16.4% (P < 0.05). These data indicate insoluble fiber reduced nutrient and energy digestibility, but xylanase was effective in partially mitigating that effect.

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 52-52
Author(s):  
Amy Petry ◽  
Nichole Huntley ◽  
Mike R Bedford ◽  
R T Zijlstra ◽  
John Patience

Abstract Xylanase should increase arabinoxylan digestibility in swine, but the responses in corn-based diets are inconsistent. The experimental objective was to investigate xylanase efficacy in growing pigs fed a diet high in insoluble corn fiber and afforded a longer adaptation period than typically reported in the literature. Sixty gilts (25.4 ± 0.9 kg BW; L337 × Camborough), were blocked by weight, housed individually, and randomly assigned to one of four dietary treatments: a low-fiber control [LF; 6.9% non-starch polysaccharides (NSP)], a 30% corn bran high-fiber control (HF; 17.6% NSP), HF + 100 mg xylanase/kg (HF+XY; Econase XT 25P; AB Vista, Marlborough, UK), and HF + 50 mg arabinoxylan-oligosaccharide/kg (HF+AX; 3-7 degrees of polymerization). Diets contained 0.5% CrO3. Gilts were fed ad libitum for 36 d, followed by 10-d of limit feeding (80% of average ad libitum intake) and were housed in metabolism crates. On d 46, pigs were necropsied and ileal, cecal, and colonic digesta were collected; ileal digesta was pooled within replicate for NSP analysis (n=6 per treatment). Data were analyzed as a linear mixed model using replicate and block as random effects, and treatment as a fixed effect. Compared to LF, the HF diet reduced (P&lt; 0.01) apparent ileal digestibility (AID) of total NSP (32.7 vs. 16.3%), insoluble NSP (50.0 vs. 21.5%), and insoluble arabinoxylan (29.8 vs. 15.4%). Xylanase (HF+XY vs. HF) increased (P&lt; 0.01) AID of total NSP by 39.8% (27.1 vs. 16.3%) and insoluble NSP by 34.0%. This may be attributed to xylanase increasing AID of total arabinoxylan by 54.5% over HF, 44.5% over LF, and 56.9% over HF+AX (33.0, 15.1, 18.3, and 14.2%, respectively; P=0.02). In conclusion, increasing insoluble fiber reduces NSP digestibility, but xylanase can increase AID of arabinoxylan and NSP. Supplementing an arabinoxylan-oligosaccharide, a potential release product of the xylanase, did not increase NSP digestibility.


2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 89-90
Author(s):  
Amy L Petry ◽  
Nichole F Huntley ◽  
Mike R Bedford ◽  
John F Patience

Abstract Xylanase may improve the utilization of insoluble fiber by the pig, but its mode of action (MOA) is incompletely understood. The experimental objective was to investigate xylanase MOA in vivo in growing pigs fed a diet higher in insoluble fiber. Sixty gilts (n = 15 pigs/treatment; 25.43 ± 0.88 kg BW; L337 X Camborough, PIC, Hendersonville, TN) were blocked by weight, housed individually, and randomly assigned to one of four dietary treatments: a low-fiber control (LF; NDF=8.45%), a 30% corn bran higher-fiber control (HF; NDF=24.5%), HF + 100 mg xylanase/kg (HF+XY; Econase XT 25P; AB Vista, Marlborough, UK), and HF + 50 mg arabinoxylan-oligosaccharide/kg (HF+AX; 3–7 degrees of polymerization). Gilts were fed ad libitum for 36 d, followed by a 10-d period of being limit fed (80% of average ad libitum intake) and housed in metabolism crates for use in a related study. Pigs and feeders were weighed on d 0 and 36. On d 46, pigs were necropsied and ileal, cecal, and colonic digesta pH were measured; serum was collected for malondialdehyde (MDA) and total antioxidant capacity (TAC) analysis. Data were analyzed using PROC MIXED (SAS; 9.4) as a randomized complete block design with pig as the experimental unit, block and replicate as random effects, and treatment as a fixed effect. Insoluble fiber reduced ADG (858 vs. 698 g/d; LF vs. HF, P < 0.001) and GF (0.433 vs. 0.353; LF vs. HF, P < 0.001), but not ADFI (P > 0.05). Xylanase, but not arabinoxylan-oligosaccharide, when compared to HF, improved ADG (762 vs. 698 g/d; P < 0.05), GF (0.382 vs. 0.353; P < 0.05), and final BW (P < 0.05). Cecal and colonic pH did not differ (P > 0.05), but LF and HF+XY reduced ileal pH (P < 0.05). Xylanase supplementation increased serum TAC (P < 0.05), and tended to reduce MDA (P = 0.098). In conclusion, xylanase supplementation improved insoluble fiber utilization. The MOA could include mitigation of reactive oxygen species.


2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 61-62
Author(s):  
Woongbi Bi Kwon ◽  
Jose A Soto ◽  
Hans H Stein

Abstract An experiment was conducted to test the hypothesis that addition of Val, Ile, or Trp alone or in combination will reduce the negative effects of excess Leu in diets for growing pigs. A total of 288 growing pigs (28.6 ± 2.5 kg) were randomly assigned to 1 of 9 dietary treatments in a randomized complete block design. There were 2 barrows and 2 gilts in each pen and 8 replicate pens per treatment. A control diet based on corn and soybean meal and 8 diets based on corn and a high-protein corn product (48% crude protein) with 2 levels of crystalline L-Val (0 or 0.10%), L-Ile (0 or 0.10%), and L-Trp (0 or 0.05%) were formulated. The crystalline L-Val, L-Ile, and L-Trp increased standardized ileal digestible (SID) Val:Lys from 70 to 80%, SID Ile:Lys from 53 to 63%, and SID Trp:Lys from 18 to 23%, respectively. All diets were formulated to contain 1.00% SID Lys and the 8 diets containing corn protein contained 171% SID Leu:Lys. Individual pig weights were recorded at the beginning and at the conclusion of the 28-d experiment. Data were analyzed using the PROC MIXED of SAS with a pen as the experimental unit. Diet was the fixed effect and block and replicate within block were random effects. Results indicated that final body weight and average daily gain were not different between pigs fed the control diet and pigs fed the diet with Val and Trp addition, but greater (P &lt; 0.001) than for pigs fed the diet with Val addition, Ile addition, Trp addition, Val and Ile addition, Ile and Trp addition, or Val, Ile, and Trp addition (Table 1). In conclusion, addition of Val and Trp to diets with excess Leu may prevent negative effects of excess Leu in diets for growing pigs.


2011 ◽  
Vol 40 (2) ◽  
pp. 370-376 ◽  
Author(s):  
Valéria Vânia Rodrigues ◽  
Vinícius de Souza Cantarelli ◽  
Nikolas de Oliveira Amaral ◽  
Márcio Gilberto Zangeronimo ◽  
Jeronimo Ávito Gonçalves de Brito ◽  
...  

Two experiments were conducted to evaluate the performance, the energy and nutrient balance and economic viability of production of growing pigs fed diets with reduced levels and phytase. In experiment 1, 60 barrows and gilts (initial weight 25 ± 1.72 kg) were housed in a randomized block design with three diets, ten replicates and two animals per experimental unit (one male and one female). One of the diets was formulated with a reduced level with phytase and evaluated in comparison to a positive-control diet that was formulated according to the nutritional recommendations for animals of this breed, and a negative-control diet without phytase. The different diets did not affect feed intake, but the diet with phytase promoted better final weight, daily weight gain and feed conversion, although it did not differ from the positive control diet. In experiment 2, 27 barrows (initial weight 49 ± 3.67 kg) were housed in random blocks with three diets and nine replicates with experimental plot represented by one animal. The diets did not affect the balance of energy and nutrients but had a significant effect on the efficiency of utilization of calcium and phosphorus, which was the lowest in animals that received the positive-control diet. Economic viability improved when nutrients were reduced and phytase was added in the ration. The addition of phytase (500 ftu/kg) in rations formulated with nutritional levels reduced for growing swines improves rearing productivity and it is economical viable.


2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 80-80
Author(s):  
Jinlong Zhu ◽  
Gerald C Shurson ◽  
Lynsey Whitacre ◽  
Ignacio R Ipharraguerre ◽  
Pedro E Urriola

Abstract The objective of this study was to determine the effects of an Aspergillus oryzae prebiotic (AOP, Amaferm®) on nutrient digestibility in growing pigs fed high fiber diets. Eighteen growing barrows (initial BW = 50.60 ± 4.90 kg) were surgically equipped with a T-cannula at the distal ileum. Three diets were formulated by including 29.65% corn-distillers dried grains with solubles (DDGS), 36.65% rice bran (RB) or 24.59% wheat middlings (WM) in corn and soybean meal-based diets to meet nutrient requirements for 50 to 75 kg growing pigs. Three additional diets were formulated by supplementing 0.05% AOP at the expense of corn in DDGS (DDGS + AOP), RB (RB + AOP), and wheat middlings (WM + AOP) diets. Pigs were allotted randomly to a triplicated 6 × 2 Youden square design with 6 diets and 2 successive periods. Feces and ileal digesta were collected for 2 d after a 21 d adaptation period, and nutrient content was analyzed to calculate apparent total tract digestibility (ATTD) and apparent ileal digestibility (AID). Standardized ileal digestibility (SID) of amino acids was calculated by correcting AID with basal endogenous amino acid losses determined from the same set of pigs. Supplementation of 0.05% AOP increased (P &lt; 0.05) ATTD of DM, GE, CP, NDF, and ash in DDGS, RB, and WM diets. Diet DE was 35 kcal/kg greater (P &lt; 0.05) in pigs fed AOP supplemented diets compared with those fed diets without AOP. Pigs fed DDGS+AOP diet had greater (P &lt; 0.05) AID of ether extract compared to those fed DDGS diet. However, supplementation of AOP did not (P &gt; 0.05) affect AID of GE, DM, CP, NDF, ash or SID of amino acids. In conclusion, supplementation of AOP in high fiber diets containing DDGS, RB, or WM increased total tract energy value and nutrient digestibility.


Author(s):  
Amy L Petry ◽  
Nichole F Huntley ◽  
Michael R Bedford ◽  
John F Patience

Abstract In theory, supplementing xylanase in corn-based swine diets should improve nutrient and energy digestibility and fiber fermentability, but its efficacy is inconsistent. The experimental objective was to investigate the impact of xylanase on energy and nutrient digestibility, digesta viscosity, and fermentation when pigs are fed a diet high in insoluble fiber (&gt;20% neutral detergent fiber; NDF) and given a 46-d dietary adaptation period. Three replicates of 20 growing gilts were blocked by initial body weight, individually housed, and assigned to 1 of 4 dietary treatments: a low-fiber control (LF) with 7.5% NDF, a 30% corn bran high-fiber control (HF; 21.9% NDF), HF+100 mg xylanase/kg [HF+XY, (Econase XT 25P; AB Vista, Marlborough, UK)] providing 16,000 birch xylan units/kg; and HF+50 mg arabinoxylan-oligosaccharide (AXOS) product/kg [HF+AX, (XOS 35A; Shandong Longlive Biotechnology, Shandong, China)] providing AXOS with 3-7 degrees of polymerization. Gilts were allowed ad libitum access to fed for 36-d. On d 36, pigs were housed in metabolism crates for a 10-d period, limit fed, and feces were collected. On d 46, pigs were euthanized and ileal, cecal, and colonic digesta were collected. Data were analyzed as a linear mixed model with block and replication as random effects, and treatment as a fixed effect. Compared with LF, HF reduced the apparent ileal digestibility (AID), apparent cecal digestibility (ACED), apparent colonic digestibility (ACOD), and apparent total tract digestibility (ATTD) of dry matter (DM), gross energy (GE), crude protein (CP), acid detergent fiber (ADF), NDF, and hemicellulose (P&lt;0.01). Relative to HF, HF+XY improved the AID of GE, CP, and NDF (P&lt;0.05), and improved the ACED, ACOD, and ATTD of DM, GE, CP, NDF, ADF, and hemicellulose (P&lt;0.05). Among treatments, pigs fed HF had increased hindgut DM disappearance (P=0.031). Relative to HF, HF+XY improved cecal disappearance of DM (162 vs. 98g; P=0.008) and NDF (44 vs. 13g; P&lt;0.01). Pigs fed xylanase had a greater proportion of acetate in cecal digesta and butyrate in colonic digesta among treatments (P&lt;0.05). Compared with LF, HF increased ileal, cecal, and colonic viscosity, but HF+XY decreased ileal viscosity compared with HF (P&lt;0.001). In conclusion, increased insoluble corn-based fiber decreases digestibility, reduces cecal fermentation, and increases digesta viscosity, but supplementing xylanase partially mitigated that effect.


2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 394-395
Author(s):  
Jongkeon Kim ◽  
Yun Yeong Jo ◽  
Beob Gyun G Kim

Abstract The objective of this study was to determine the digestible energy (DE) and metabolizable energy (ME) concentrations in high-fiber ingredients fed to growing pigs. Twelve barrows with an initial body weight of 57.5 kg (SD = 5.7) were individually housed in metabolism crates. A replicated 6 × 3 incomplete Latin square design with 12 animals, 6 experimental diets and 3 periods was employed. A basal diet was composed of 75.0% corn and 22.7% soybean meal (SBM) as the sole energy sources. Four experimental diets were prepared by replacing 40% of corn and SBM with soybean hulls (SH), corn gluten feed (CGF), wheat bran (WB), or rice bran (RB). An additional diet was prepared by replacing 10% of corn and SBM with cashew nut hulls (CNH). Each period consisted of a 4-d adaptation period and a 4-d collection period, and the marker-to-marker procedure was used for total collection of feces and urine. The DE and ME values in RB (3,969 and 3,936 kcal/kg DM) were greater (P &lt; 0.05) than those in CGF (2,654 and 2,520 kcal/kg DM) and SH (2,492 and 2,541 kcal/kg DM) and the energy values in WB (3,162 and 3,118 kcal/kg DM) were not different from those in RB, CGF, or SH. The DE and ME values in CNH (350 and 572 kcal/kg DM) were less (P &lt; 0.05) than those in all other test ingredients. In conclusion, energy concentrations in RB were greatest among the high-fiber test ingredients, whereas CNH had the lowest values.


1985 ◽  
Vol 36 (3) ◽  
pp. 483 ◽  
Author(s):  
JK Egan ◽  
PT Doyle

Six mature Merino sheep received three treatments in a randomized block design experiment. The treatments were: chopped oaten hay diet at 90% of ad libitum intake without urea (L); the ciet offered at the same level as for L with urea infused into the rumen at 11.5 g kg-1 dry matter intake (LU); and the diet offered at 90% of the ad libitum intake achieved with urea infused at 11.5 g kg-1 dry matter intake (HU). Sheep given HU consumed 37% more (P < 0.01) organic matter (OM) than those fed L or LU, but the apparent digestibility of OM did not vary (59.2-61.8%) between treatments. The addit onal food consumption was associated with c. 20% increase (P < 0.05) in the weight of OM in the reticulorumen and significantly higher (by 10-35%; P < 0.05) fractional outflow rates of most dietary and microbial constituents of digesta. The fractional digestion rate of potentially digestible plant cell walls was not affected by urea, but the flow of microbial non-ammonia nitrogen from the abomasum was enhanced (L, 7.0; LU, 8.2; HU, 12.5 g day-1; P < 0.05). The results of this study suggest that the stimulatory effect of urea upon food intake was associated with the provision of additional microbial protein for digestion in the intestines, rather than changes in the rate or extent of organic matter fermentation in the reticulorumen.


2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 95-95
Author(s):  
Cecilia Winfrey ◽  
Merlin D Lindemann ◽  
James Matthews

Abstract The hypotheses tested were that (a) summer-long (84 d) grazing of endophyte-infected tall fescue (TE) would alter the count of circulating leukocytes (WBC) and WBC expression of key prolactin and NF-kB pathway genes and (b) that ad libitum intake of an 1:1 blend (MIX) sodium selenite (ISe) and SELPLEX vs ISe in a vitamin-mineral mix (V-M, 27 ppm Se) would ameliorate negative TE effects. Sixteen TE-naïve beef steers depleted of Se were randomly assigned to ad libitum consumption of ISe vs MIX for 35 d. Within Se-form treatments, 2 steers (ISe = 316±37 kg, MIX = 314±39 kg) were randomly assigned to graze each of 4, 2-acre TE paddocks for 84 d and had ad libitum access to their respective V-M. Paddock was the experimental unit (n = 4). The MIXED procedure of SAS was used to assess effect of grazing TE (d 0 vs 84), Se-form (ISe vs MIX), and their interaction on clinical parameters WBC, and relative mRNA content (RT-PCR) in blood. Whole blood Se was 24% greater (P &lt; 0.01) for MIX steers and decreased (P &lt; 0.01) 34% by d 84. Serum prolactin decreased 88% by d 84 and was 98% greater (P &lt; 0.01) for MIX steers on d 84. Monocytes decreased (P &lt; 0.01) 47% with TE and were 67% lower (P = 0.04) in MIX steers. Grazing of TE altered mRNA content of CISH, STA5B, PPIB, AGAP2, and SPRLR; and IL-8, RELA, NFKBIA, TLR, IKBKG, ZAP70, and LCK; of the prolactin (P ≤ 0.05) and NF-kB (P ≤ 0.04) pathways, respectively. In contrast, Se form had no effect (P ≥ 0.07) on mRNA content. Summer-long consumption of TE by steers altered monocyte numbers and expression of prolactin and NF-kB genes, but ad libitum intake of MIX Se forms did not ameliorate these effects.


2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 198-198
Author(s):  
Ricardo M Garcia ◽  
Rhuan Chaves ◽  
Gabriel Arruda ◽  
Maíra Resende ◽  
Jéssica Barbosa ◽  
...  

Abstract Prebiotics has been used in sow diets as an alternative to minimize the impacts of hyperprolificity, such as variability coefficient and poor litter performance. However, the additive inclusion is generally high and costly. The objective of this study was to verify the effect of the low inclusion of yeast bioactive compounds (YBC), in gestation and lactation diets, on sows reproductive performance. Five hundred sows were assigned in two treatments in a block design considering parity as a random factor, using each sow as an experimental unit with for gestation (n = 500) and lactation period (n = 80). The treatments were control diet (CON) and diet with 0.036% of YBC inclusion (YBC). Back fat thickness was measured at 30 and 70 days of gestation, at farrowing day, at 14 days of lactation and weaning day. Sows were weighted at the farrowing barn entry day and at weaning. The corporal mobilization was accessed following the equation: Body change = Weight at weaning - [Weight at lactation entry - (Litter weight at birth + Placenta weight)]. After cross-fostering and at weaning the litters were weighed to calculate the daily weight gain. Differences were considered significant when p ≤ 0.05 and tendencies were considered when p > 0.05 and p < 0.10. No difference was found regarding the reproductive performance (total born, born alive, mummified and stillborns) (P > 0.05). YBC tended to have greater litter final weight than CON group, 59.08 kg versus 56.70 kg (P = 0.057). Backfat thickness tended to be lower (5.20%) in YBC (16.03 mm) than CON group (16.91 mm) during gestation period (P = 0.075). However, at weaning day no difference for backfat thickness was found between treatments (P > 0.05). YBC had lower loss weight during lactation than CON group, 0.0 kg versus 4.99 kg, respectively (P = 0.011). Low inclusion of YBC enhances litter performance and improves body condition of lactating sows.


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