scholarly journals 130 Comparison of amino acid digestibility in fish meal, porcine plasma, porcine red blood cell, and blood meal between broiler chickens and young pigs

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 112-113
Author(s):  
Chan Sol Park ◽  
Victor Naranjo ◽  
John K Htoo ◽  
Olayiwola Adeola
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Blood Meal ◽  
Broiler Chickens ◽  
Fish Meal ◽  
Block Design ◽  
Latin Square ◽  
Experimental Period ◽  
High Concentration ◽  
Ileal Digestibility

Abstract The objective of this study was to compare the standardized ileal digestibility (SID) of AA in fish meal (FM), porcine plasma (PP), porcine red blood cell (RBC), and blood meal (BM) between broiler chickens and pigs. Five diets were prepared for both species including 4 diets containing test ingredients and a nitrogen-free diet. In Exp. 1, 416 male broiler chickens (initial BW = 705 ± 100.0 g) at d 18 post hatching were assigned to diets in a randomized complete block design with BW as a blocking factor. After 5 d of feeding, birds were euthanized for collection of ileal digesta. In Exp. 2, 10 barrows (initial BW = 22.1 ± 1.54 kg) surgically fitted with T-cannulas at the distal ileum were allotted to duplicate 5 × 4 incomplete Latin square design with 5 diets and 4 periods. Each experimental period consisted of 5-d adaptation and 2-d ileal digesta collection periods. Data for Exp. 1 and 2 were considered together as a 2 × 4 factorial arrangement with the effects of species (i.e., broiler chickens and pigs) and four diets (i.e., FM, PP, RBC, and BM). There were interactions (P < 0.05) between species and diets on the SID of AA except for Pro. The SID of Lys, Met, Thr, and Trp in FM, PP, and RBC for broiler chickens were not different from values for pigs; however, the SID of respective AA in BM for broiler chickens were less than values for pigs. The SID of indispensable AA in BM were less than in FM, PP, or RBC within each species, but the SID of indispensable AA in BM for broiler chickens was less than values for pigs. In conclusion, utilization of AA in animal protein ingredients containing high concentration of CP varies between broiler chickens and pigs.

scholarly journals 118 Digestibility of amino acids in hydrolyzed feather meal, flash dried poultry protein, poultry meal, and meat and bone meal fed to broiler chickens and pigs

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 65-65
Author(s):  
Chan Sol Park ◽  
Victor Naranjo ◽  
Ariane Helmbrecht ◽  
John K Htoo ◽  
Olayiwola Adeola
Keyword(s):  
Amino Acids ◽  
Broiler Chickens ◽  
Block Design ◽  
Latin Square ◽  
Experimental Period ◽  
Meat And Bone Meal ◽  
Feather Meal ◽  
Bone Meal ◽  
Ileal Digestibility ◽  
Randomized Complete Block Design

Abstract This study was conducted to determine the standardized ileal digestibility (SID) of amino acids (AA) in hydrolyzed feather meal (HFM), flash dried poultry protein (FDPP), poultry meal (PM), and meat and bone meal (MBM) fed to broiler chickens and pigs. Four semi-purified diets and a nitrogen-free diet were prepared. Exp. 1 was conducted with 416 male broiler chickens (initial body weight [BW] = 705 ± 100.0 g) at d 18 post-hatching in a randomized complete block design. Birds were fed diets for 5 d and then euthanized to collect ileal digesta. In Exp. 2, ten barrows (initial BW = 22.1 ± 1.59 kg) surgically fitted with T-cannulas at the distal ileum were assigned to duplicate 5 × 4 incomplete Latin square design with 5 diets and 4 periods. There were 5-d adaptation and 2-d ileal digesta collection periods in each experimental period. Data were analyzed as a 2 × 4 factorial arrangement with the effects of species (i.e., broiler chickens and pigs) and four experimental diets. There were interactions (P < 0.05) in the SID of His, Lys, Thr, Trp, and Val between diets and species. In broiler chickens, the SID of Lys in MBM was greater (P < 0.05) than HFM and FDPP but was not different from PM. Pigs fed MBM had greater (P < 0.05) SID of Lys than HFM, FDPP, and PM. In broiler chickens, the SID of His, Thr, and Trp in FDPP and PM were greater (P < 0.05) than HFM but was less (P < 0.05) than MBM. In pigs, the SID of His, Thr, and Trp in FDPP and PM were greater (P < 0.05) than HFM but was not different from MBM. In conclusion, differences in the SID of AA among animal protein sources were affected by species of monogastric animals.

scholarly journals Comparative amino acid digestibility between broiler chickens and pigs fed different poultry by-products and meat and bone meal

2020 ◽  
Vol 98 (7) ◽  
Author(s):  
Chan Sol Park ◽  
Victor Daniel Naranjo ◽  
John Kyaw Htoo ◽  
Olayiwola Adeola
Keyword(s):  
Broiler Chickens ◽  
Block Design ◽  
Latin Square ◽  
Sole Source ◽  
The Other ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Ileal Digestibility ◽  
Collection Data ◽  
By Products

Abstract The objective of this study was to compare the standardized ileal digestibility (SID) of amino acids (AA) in 3 poultry by-products including hydrolyzed feather meal (HFM), flash dried poultry protein (FDPP), and poultry meal (PM) and also a meat and bone meal (MBM) between broiler chickens and pigs. Experimental diets consisted of 4 diets containing each test ingredient as a sole source of nitrogen and a nitrogen-free diet. In experiment 1, 416 male broiler chickens with a mean initial body weight (BW) of 705 ± 100 g were allotted to 5 diets with 8 replicate cages per diet in a randomized complete block design with BW as a blocking factor at day 18 posthatching. After 5 d of feeding experimental diets, birds were euthanized by CO2 asphyxiation, and ileal digesta samples were collected from distal two-thirds of the ileum. In experiment 2, 10 barrows with a mean initial BW of 22.1 ± 1.59 kg were surgically fitted with T-cannulas at the distal ileum and allotted to a duplicate 5 × 4 incomplete Latin Square design with 5 diets and 4 periods. Each period lasted for 7 d including 5 d of adaptation and 2 d of ileal digesta collection. Data from experiments 1 and 2 were pooled together and analyzed as a 2 × 4 factorial arrangement with the effects of species (broiler chickens or pigs) and 4 experimental diets (HFM, FDPP, PM, or MBM). There were interactions (P &lt; 0.05) between experimental diets and species in the SID of His, Lys, Thr, Trp, Val, and all dispensable AA except Tyr. In broiler chickens, the SID of Lys in FDPP (73.3%) was greater (P &lt; 0.05) than in HFM (55.7%) but was lower (P &lt; 0.05) than in MBM (86.5%), which was not different from PM (78.7%). In pigs, however, the SID of Lys in FDPP and PM (70.0 and 70.1%, respectively) were greater (P &lt; 0.05) than in HFM (39.0%) but were lower (P &lt; 0.05) than in MBM (79.2%). Broiler chickens fed FDPP and PM had lower (P &lt; 0.05) SID of His, Thr, and Trp than those fed MBM; however, there was no difference in the SID of His, Thr, or Trp among pigs fed FDPP, PM, or MBM. The SID of Val in MBM was greater (P &lt; 0.05) than in the other test ingredients for broiler chickens, but there was no difference in the SID of Val among test ingredients for pigs. Pigs had greater (P &lt; 0.05) SID of Ile and Met than broiler chickens. In conclusion, the pattern of differences in the SID of His, Lys, Thr, Trp, and Val, but not the other indispensable AA, among poultry by-products and MBM were different between broiler chickens and pigs.

Variability of apparent ileal amino acid digestibility in different pea samples for growing-finishing pigs

1999 ◽  
Vol 79 (4) ◽  
pp. 467-475 ◽  
Author(s):  
M. Z. Fan ◽  
W. C. Sauer
Keyword(s):  
Amino Acid ◽  
Crude Protein ◽  
Corn Starch ◽  
Latin Square ◽  
Experimental Period ◽  
Trypsin Inhibitor Activity ◽  
Significant Finding ◽  
Finishing Pigs ◽  
Initial Body Weight ◽  
Ileal Digestibility

Six barrows, average initial body weight 32.5 kg, fitted with a simple T-can-nula at the distal ileum, were used to investigate factors responsible for the variability of apparent ileal amino acid (AA) digestibility values among six pea samples. The barrows were fed six corn-starch-based diets, formulated to contain 16.5% crude protein (CP) (as-fed) from six different pea samples, according to a 6 × 6 Latin square design. Chromic oxide (0.3%) was used as the digestibility marker. The pigs were fed twice daily, at 0800 and 2000 h. The dietary allowance was 1600 g−1. Each experimental period lasted 7 d. Ileal digesta were collected at 2-h intervals for a total of 24 h from 0800 h on day 6 to 0800 h on day 8. With the exception of arginine, cysteine, histidine, and methionine, there were differences (P < 0.05) in the apparent ileal digestibility values of the indispensable including semi-essential (+semi-) AA among the pea samples. Of the indispensable AA (+semi-) within each pea sample, the digestibility values of arginine and lysine were relatively high, ranging from 88.3 to 91.3% and from 78.7 to 85.2%, respectively, while the digestibility values of cysteine, methionine, and tryptophan were relatively low, ranging from 53.8 to 62.7%, from 69.4 to 75.4%, and from 53.1 to 70.4%, respectively. With the exception of arginine, cysteine, and tryptophan, the ileal digestibility values of the indispensable AA (+semi-) were negatively correlated (P < 0.05) with the neu-tral-detergent fiber content in the pea samples. Of all the AA, only the digestibility of tryptophan was negatively correlated (P < 0.05) with the trypsin inhibitor activity in the pea samples. The significant finding of this study was that with the exception of arginine, cysteine, and proline, differences in the digestibility values of all other AA in the pea samples were positively correlated (P < 0.05) with their respective dietary AA contents. This suggests that differences in AA levels in the assay diets were responsible for the variability of apparent ileal digestibility values of AA between pea samples. Therefore, to eliminate this methodological effect, true rather than apparent ileal AA digestibility values for peas should be determined. Key words: Amino acids, ileal digestibility, peas, pigs

scholarly journals PSI-12 Effect of a carbohydrase mixture on apparent and standardized ileal digestibility of amino acids in dry extruded-expelled soybean meal fed to growing pigs

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 292-293
Author(s):  
Jichen Song ◽  
Ahmed Aljuobori ◽  
C Martin Nyachoti ◽  
Gustavo A Mejicanos
Keyword(s):  
Soybean Meal ◽  
Mixed Model ◽  
Latin Square ◽  
Experimental Period ◽  
Sole Source ◽  
Antinutritional Factors ◽  
Nutrient Digestibility ◽  
Growing Pigs ◽  
Mean Values ◽  
Ileal Digestibility

Abstract Heat treatment could effectively decrease the antinutritional factors in soybean during meal processing. One such progress is the combination of extrusion with expelling, which produces the dry extruded-expelled soybean meal (DESBM), and there is a great interest in exploring its utilization as a dietary ingredient for swine. Thus, eight ileal-cannulated barrows (initial BW = 30 ± 1.3 kg) were used to determine the ileal amino acid (AA) digestibility of DESBM fed to growing pigs with or without multi-carbohydrase (MC) supplementation. Pigs were randomly assigned to 1 of 4 treatments in a replicated 4 × 4 Latin square design to give eight observations per treatment. The experimental diets were formulated to contain one of the two batches of DESBM (DESBM-1 and DESBM-2) as the sole source of protein. All diets contained titanium dioxide (0.3%) as an indigestible marker to calculate nutrient digestibility. Each experimental period lasted for seven days, and ileal digesta samples were collected on d 6 and d 7. The standardized ileal digestibility (SID) of AA were calculated using published mean values to estimate ileal endogenous AA losses. Data were analyzed using the mixed model procedures of SAS, and the final model had treatment, batch, and the interaction between treatment and batch as the main effects. In general, the SID of AA in DESBM-1 and DESBM-2 were not different, such as Ile, Lys, Met, Thr, and Val, which averaged 88.97%, 89.77%, 89.08%, 84.38%, and 86.85% respectively. There were no effects of MC supplementation on AID and SID of AA digestibility in DESBM except for the AID of Trp (P &lt; 0.05). Also, there were no interaction effects of MC supplementation and batch on AA digestibility in DESBM. In conclusion, enzyme supplementation did not improve the AID and SID of AA in DESBM fed to growing pigs.

scholarly journals Crystalline amino acids do not influence calculated values for standardized ileal digestibility of amino acids in feed ingredients included in diets for pigs

2020 ◽  
Vol 98 (11) ◽  
Author(s):  
Maryane S F Oliveira ◽  
Jerubella J Abelilla ◽  
Neil W Jaworski ◽  
John K Htoo ◽  
Hans H Stein
Keyword(s):  
Amino Acids ◽  
Latin Square ◽  
Experimental Period ◽  
Adaptation Period ◽  
Ileal Digestibility ◽  
Entire Experiment ◽  
Collection Period ◽  
Dietary Treatments ◽  
Feeding Diets ◽  
Crystalline Amino Acids

Abstract An experiment was conducted to test the hypothesis that addition of crystalline amino acids (AA) to diets during the adaptation or collection periods will not influence calculated values for apparent ileal digestibility (AID) or standardized ileal digestibility (SID) of AA in corn and soybean meal (SBM). Seven ileal-cannulated barrows (initial body weight: 77.9 ± 2.6 kg) were allotted to a 7 × 7 Latin Square design with 7 dietary treatments and 7 periods. Dietary treatments included feeding diets based on corn or SBM without or with crystalline AA for the entire 7-d period or with crystalline AA during the adaptation period, but without crystalline AA during the collection period. An N-free diet was also used. Each experimental period consisted of 5 d of adaptation to the diets followed by 2 d of ileal digesta collection, with digesta being collected for 9 hr/d starting after feeding the morning meal. Thus, the entire experiment lasted 49 d. The AID and SID of crude protein (CP) and AA were calculated using values determined in corn or in SBM without or with crystalline AA to determine if crystalline AA influenced calculated values for AID or SID of CP and AA. Results indicated that addition of crystalline AA to diets fed during the entire 7-d period increased (P &lt; 0.05) AID of some AA in corn and SBM. However, no differences in SID of CP and AA were observed between pigs fed the diets without crystalline AA and pigs fed the diets with crystalline AA if dietary crystalline AA were ignored in calculations of SID values. This indicates that crystalline AA were 100% absorbed before the distal ileum and did not affect calculated values for SID of AA if calculations were based only on the AA in corn or SBM. Therefore, it is concluded that crystalline AA may be added to experimental diets in digestibility experiments before and during collection periods without affecting results, if crystalline AA are disregarded in the calculation of AID or SID of AA in ingredients.

scholarly journals DUCK EGGWHITE POTENTIAL IN TREATING SUBACUTE LEAD POISONING

2017 ◽  
Vol 10 (14) ◽  
pp. 98
Author(s):  
Aulia Andi Mustika ◽  
Andriyanto Andriyanto ◽  
Lina Noviyanti Sutardi ◽  
Meilisa L Margarita
Keyword(s):  
Blood Cell ◽  
Lead Poisoning ◽  
Red Blood Cell ◽  
Clinical Signs ◽  
Egg White ◽  
High Concentration ◽  
Treatment Groups ◽  
Cell Counts ◽  
Duck Egg ◽  
Cell Profile

Objectives: The aim of this research is to study duck eggwhite potential in treating subacute lead poisoning.Methods: The potential of duck egg white was evaluated from the red blood cell profile and the clinical signs that emerged. The research used thirtymale rats which were divided into 6 groups and 5 replications (i.e. rats as control, rats dministrated only with lead, rats administrated with leadand antidote). Each of the four treatment groups were given one antidote (i.e. EDTA, 50% egg white, 75% egg white, and 100% egg white). Leadforce feeding was conducted for 15 days, followed by the administration of the antidote for the same duration of 15 days, and concluded with bloodsampling at the end of each treatment.Results: There was no significant effect on haemoglobin but lead decreased total red blood cells (p<0.05) in subacute lead poisoning. Rats that weregiven 75% and 100% duck egg white as an antidote showed an increase in total red blood cell counts in addition to a faster recovery.Conclusion: High concentration of duck egg white had shown positive results as an antidote for subacute lead poisoning. 

EVALUATION OF TWO ANIMAL PROTEIN SOURCES IN SINGLE PHASE FEEDING OF BROILER CHICKENS

2019 ◽  
Vol 18 (1) ◽  
pp. 159-168
Author(s):  
U. OKAH ◽  
G. C. OKEKE ◽  
A. I. UKANWOKO ◽  
T. B. ADEWOLE
Keyword(s):  
Weight Gain ◽  
Blood Meal ◽  
Broiler Chickens ◽  
Fish Meal ◽  
Control Diet ◽  
Cost Benefit ◽  
Diet Group ◽  
Animal Protein ◽  
Feed Conversion ◽  
Protein Sources

A study was carried out to compare the use of blood meal and fish meal as protein sources in single phase feeding of broiler chickens.  Three isonitrogenious and isocaloric diets containing between 20.5 to 21.1 % CP and 3440 to 3454 kcal/kg ME were formulated. The control diet contained none of the animal protein sources while the other two diets contained blood meal and fish meal at 6%, respectively. After six weeks of straight feeding, data were collected on feed intake, weight gain and feed conversion ratio was calculated. Carcass characteristics and cost benefit were also calculated. The results indicated that birds fed fish meal based diet  consumed more feed (p< 0.05) than those on control diet,  while intake of those on blood meal based diet  was an interphase between the control and fish meal based diet groups. The birds fed fish meal based diet also recorded higher (p< 0.05) daily weight gain than those on control and blood meal based diets. Feed conversion ratio was better in the group fed fish meal based diet than those fed the control diet, but similar (p> 0.05) to the group fed the blood meal based diet. The dressing percentage of the birds was similar (p>0.05) in the control and blood meal based diet groups, but they were higher (p<0.05) than the fish meal based diet group. The neck, back, drumstick and head were significantly (p<0.05) higher in the control group than in the groups containing animal protein. Cost benefit analysis showed that feed cost and cost per kg weight gain were higher (p< 0.05) in fish meal based diet group than in control and blood meal based diet groups. Revenue per bird and gross margin were higher (p< 0.05) in the blood meal based diet group than in the control diet and fish meal based diet groups. In conclusion, fast growing broiler chickens for eatery houses can be better reared with a single diet of 21 % CP and 3440 kcal/kg ME using blood meal as a protein source.   Keywords: , , , , , ,  

Haemoglobin H+ equilibria in lamprey (Lampetra fluviatilis) and hagfish (Myxine glutinosa)

1999 ◽  
Vol 202 (14) ◽  
pp. 1963-1968
Author(s):  
F.B. Jensen
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Carrying Capacity ◽  
Venous Blood ◽  
Evolutionary Divergence ◽  
High Concentration ◽  
High Co2 ◽  
Virtual Absence ◽  
Fixed Acid ◽  
Haldane Effect

Agnathans, comprising lamprey and hagfish species, have been reported to be practically devoid of HCO3-/Cl- exchange across the red blood cell membrane. This suggests that the capacity of their haemoglobin (Hb) to remove H+ is essential for obtaining a high CO2-carrying capacity in the blood. Hydrogen ion titrations were performed on oxygenated and deoxygenated composite Hbs from river lamprey and from Atlantic hagfish at 15 degrees C and an ionic strength of 0.1 (0.1 mol l-1 KCl). Lamprey Hb was characterised by very low buffer values when the degree of oxygenation was constant, whereas the fixed-acid Haldane effect was large (uptake of approximately 0.9 H+ per monomer upon deoxygenation). Hagfish Hb, in contrast, had large buffer values and a moderate fixed-acid Haldane effect. In deoxygenated Hb, the low buffer values in lamprey correlated with the presence of only 1–1.5 titratable ‘neutral’ groups (normally histidines and α -amino groups) per monomer, whereas there were 4–5 titratable ‘neutral’ groups per monomer in hagfish. The large differences in Hb/H+ equilibria between the two species reflect the early evolutionary divergence between lampreys and hagfish. With respect to CO2 transport, the special Hb/H+ equilibria and the high red blood cell pH in lamprey ensure a high concentration of free HCO3- inside the red cells in venous blood, which compensates for the absence of a shift of HCO3- to the plasma. The Hb/H+ equilibria in hagfish are less effective in ensuring a high CO2-carrying capacity given the virtual absence of a red blood cell HCO3-/Cl- exchange, and other adaptations may be involved.

scholarly journals Effect of T-2 and HT-2 Toxin during the Growing Period on Body Weight, Lipid Peroxide and Glutathione Redox Status of Broiler Chickens

2010 ◽  
Vol 79 (1) ◽  
pp. 27-31 ◽  
Author(s):  
Mária Weber ◽  
Krisztián Balogh ◽  
Judit Fodor ◽  
Márta Erdélyi ◽  
Zsolt Ancsin ◽  
...  
Keyword(s):  
Body Weight ◽  
Glutathione Peroxidase ◽  
Blood Cell ◽  
Blood Plasma ◽  
Red Blood Cell ◽  
Peroxidase Activity ◽  
Broiler Chickens ◽  
Reduced Glutathione ◽  
Liver Homogenate ◽  
Glutathione Peroxidase Activity

The effect of T-2 and HT-2 toxin using different doses in the starter (0-21 days: 1.04 mg T-2 toxin and 0.49 mg HT-2 toxin kg-1feed), and finisher diets (22-39 days: 0.12 mg T-2 toxin and 0.02 mg HT-2 toxin kg-1feed) was investigated in broiler chickens. Birds were divided into two groups fed with control and T-2 and HT-2 toxin contaminated diets. Pathological signs of toxicity were investigated on days 21 and 39 of the trial, individual liveweight was measured weekly. Five birds from each group were sacrificed on the 21st and 39th days of treatment, when blood plasma, red blood cell, liver and kidney samples were taken, in which malondialdehyde and reduced glutathione concentration and glutathione-peroxidase activity were determined. Pathological signs (lesions in the oral cavity and on the tongue, inflammation in the small intestine) were found in the group fed T-2 and HT-2 toxin contaminated feed on day 21 compared to control. Body weight was significantly lower as a result of feeding T-2 and HT-2 toxin contaminated diet. However, the contamination did not cause a significant increase of malondialdehyde content in the analysed tissues. Reduced glutathione content was significantly lower in the liver homogenate on day 39 than that of the control. Glutathione peroxidase activity also did not differ significantly in blood plasma, red blood cell haemolysates and kidney homogenates, while it was significantly higher in the liver homogenates of the mycotoxin-challenged birds. In conclusion, it can be stated that T-2 and HT-2 toxin exposure has long-term effects in broiler chickens.

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