scholarly journals PSI-26 Effects of low-moisture, sugarcane molasses-based block supplementation on feed intake and ingestive behavior of Nellore steers fed low-quality forage

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 250-250
Author(s):  
Andresa L Feliciano ◽  
Sérgio A G Pereira-Junior ◽  
Yury Granja-Salcedo ◽  
Livia Maria Sampaio Ferraz Sepini de Souza Grilo ◽  
Luís Felipe Arelaro Artioli ◽  
...  
Keyword(s):  
Feeding Behavior ◽  
Random Effect ◽  
Latin Square ◽  
Experimental Period ◽  
Ingestive Behavior ◽  
Feeding Time ◽  
Sugarcane Molasses ◽  
Standard Value ◽  
Trained Observers ◽  
Corn Grain

Abstract We evaluated the effects of low-moisture, sugarcane molasses-based block supplementation (LMB) on dry matter and nutrients intake and measurements of feeding behavior of steers fed low quality forage. Six rumen cannulated Nellore steers (23 months, 350 ± 10 kg) were distributed in a 3 × 3 double Latin square design. The treatments were Brachiaria brizantha cv. Marandu hay ad libtum as an exclusive source of bulky and supplements: complete mineral blend with urea [UR, (urea, salt, mineral-vitamin premix)], a commercial protein supplement [PS, (corn grain, soybean meal, urea, salt and mineral-vitamin premix)] or low-moisture, cooked sugarcane molasses-based protein block [LMB, (cane molasses, cottonseed meal, soybean oil, urea, salt and mineral-vitamin premix)]. Each experimental period (3) lasted 21 days (14 days of adaptation and 7 days of data collection). The animals were fed twice a day (7:00 a.m. and 3:00 p.m.) and the offered and refused hay were weighed every morning to calculate DM and NDF intake. On feeding behavior measurements, trained observers assesed the animals behavior at 5-minute intervals for 24 hours. We evaluated the feeding time, water intake, rumination, idle and others. The rumination parameters and the average chewing time were observed using digital timer. The data were analyzed using Software R, having as fixed effect the treatments and as animal random effect, period, Latin square and error. The hay (P = 0.024), Total DM (g/day, P = 0.001 and % BW, P = 0.001), NDF (P = 0.027) and supplement (P < 0.001) intake was greater for animals supplemented with PS, besides the feeding time (P < 0.001). While no effect was observed on the other measurements. In summary, none of the supplements were able to increase DMI to its standard value of around 2 % of BW. Supplementation with LMB did not alter the feeding behavior in Nellore steers.

scholarly journals PSI-27 Excretion of purine derivatives and rumen microbial protein synthesis in Nelore steers supplemented with low-moisture, sugarcane molasses-based block and fed low-quality forage

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 249-250
Author(s):  
Andresa L Feliciano ◽  
Sérgio A G Pereira-Junior ◽  
Yury Granja-Salcedo ◽  
Livia Maria Sampaio Ferraz Sepini de Souza Grilo ◽  
Luís Felipe Arelaro Artioli ◽  
...  
Keyword(s):  
Random Effect ◽  
Latin Square ◽  
Experimental Period ◽  
Sugarcane Molasses ◽  
Microbial Protein ◽  
Purine Derivatives ◽  
Microbial Nitrogen ◽  
Protein Supply ◽  
Protein Block ◽  
Corn Grain

Abstract The objective of this study was to determine the effects of supplementation of low-moisture, sugarcane molasses-based block (LMB) on steers fed low quality forage in the excretion of purine derivatives and in the synthesis of ruminal microbial protein. Six rumen cannulated Nellore steers steers (23 months, 350 ± 10 kg) were distributed in a 3 × 3 double Latin square design. The treatments were composed of Brachiaria brizantha ‘Marandu’ hay ad libtum as an exclusive source of bulky (93.65% DM, 3.97% CP and 81.76% NDF) and supplements: complete mineral blend with urea [UR, (urea, salt, mineral-vitamin premix)], a commercial protein supplement [PS, (corn grain, soybean meal, urea, salt and mineral-vitamin premix)] or low-moisture, cooked sugarcane molasses-based protein block [LMB, (cane molasses, cottonseed meal, soybean oil, urea, salt and mineral-vitamin premix)]. Each experimental period lasted 21 days (14 days of adaptation and 7 days of data collection). The total urinary volume was measured for five days in each experimental period. The urine was collected in rubber funnels fixed by elastic loops on the backs of the animals. The urine was conduct through hoses connected to a 20 L polyethylene bucket containing 250 mL of 20% H2SO4 solution. Every 24 hours, the collected urine was homogenized and the total excreted volume was measured. The data were analyzed using Software R, having as fixed effect the treatments and as animal random effect, period, Latin square and error. Supplementation with LMB lead to greater excretion of allantoin (P = 0.046), microbial nitrogen flow (P = 0.023) and higher microbial crude protein (P = 0.023) into the intestine compared to UR and PS. While no effect was observed on total purines and purines absorbed (P > 0.05). Thus, LMB supplementation for rumen cannulated Nellore steers fed low quality forage was effective to improve metabolizable protein supply.

scholarly journals PSI-28 Effect of supplementation with low-moisture, sugarcane molasses-based block on intake and ruminal volatile fatty acid profile on steers fed low quality forage

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 250-251
Author(s):  
Andresa L Feliciano ◽  
Sérgio A G Pereira-Junior ◽  
Yury Granja-Salcedo ◽  
Livia Maria Sampaio Ferraz Sepini de Souza Grilo ◽  
Luís Felipe Arelaro Artioli ◽  
...  
Keyword(s):  
Rumen Fluid ◽  
Random Effect ◽  
Daily Intake ◽  
Latin Square ◽  
Experimental Period ◽  
Adaptation Period ◽  
Sugarcane Molasses ◽  
Block Based ◽  
Protein Block ◽  
Corn Grain

Abstract We evaluated the effects of supplementation of molasses based on low moisture molasses (LMB) on steers fed low quality forage in DM intake and ruminal VFA. Six rumen cannulated Nellore steers (23 months, 350 ± 10 kg) were distributed in a 3 × 3 double Latin square design. The treatments were composed of Brachiaria brizantha cv. Marandu ad libtum as an exclusive source of bulks (93.65% DM, 3.97% CP and 81.76% NDF) and supplements: complete mineral blend with urea [UR, (urea, salt, mineral-vitamin premix)], a commercial protein supplement [PS, (corn grain, soybean meal, urea, salt, and mineral- vitamin premix)] or protein block based on low-moisture cooked sugarcane molasses [LMB, (cane molasses, cottonseed meal, soybean oil, urea, salt and mineral-vitamin premix)]. Before subsequent feeding, samples of orts were collected to monitor the daily intake. After 14-d adaptation period, rumen fluid samples were collected in each experimental period at 0, 2, 4, 6, 8, 10, 12 and 14 h after feeding, to evaluate concentrations of VFA, which was determined by gas chromatography. The data were analyzed using Software R, with measures repeated in time for VFA and having as fixed effect the treatments and as animal random effect, period, Latin square and error. The intake of hay (P = 0.024), total DM (g/day, P = 0.001), NDF (P = 0.027) and supplement (P < 0.001) was higher for animals supplemented with PS. The total VFA and acetate ratio were not affected by the treatment (P > 0.05). The propionate ratio was higher for LMB (P = 0.016). The butyrate ratio (P = 0.005), valerate (P = 0.010) and A:P ratio (P = 0.017) was higher when the animals were supplemented with PS. The results indicate that the use of LMB improves propionate ratio and decrease the acetate:propionate ratio for steers fed low quality forage.

scholarly journals Feeding behavior of lambs fed castor mealom farelo de mamona

2016 ◽  
Vol 37 (3) ◽  
pp. 1451 ◽  
Author(s):  
Hellenn Cardoso Oliveira ◽  
Rasmo Garcia ◽  
Vitor Visintin Silva de Almeida ◽  
Aline Cardoso Oliveira ◽  
Aureliano José Vieira Pires ◽  
...  
Keyword(s):  
Feeding Behavior ◽  
Soybean Meal ◽  
Feed Efficiency ◽  
Dry Matter ◽  
Feeding Activity ◽  
Experimental Period ◽  
Feeding Time ◽  
Adaptation Period ◽  
Four Levels ◽  
Castor Meal

The experiment aimed to evaluate the feeding behavior of Santa Inês lambs fed diets containing different levels of detoxified castor meal in the concentrate. Twenty-four sheep with an average body weight of 18.5± 2.26 kg, at four months of age, were distributed in a randomized complete design with four treatment and six replications. Treatments consisted of four levels of castor meal (0, 33, 67, and 100%) replacing soybean meal. Animals underwent a 15-day adaptation period and an experimental period of 84 days. The diet was composed of 60% sugarcane silage and 40% concentrate, on a dry matter basis. Animal behavior (idle, rumination, and feeding activities) were observed visually for two 24-h periods with 5-min intervals, and recorded. The number of rumination chews and the time taken to ruminate each cud per day were counted using a digital stopwatch. Dry matter (DM) intake was not affected by inclusion of castor meal in the concentrate, averaging 884.02 g day?1. The feeding activity was not affected by addition of castor meal. Rumination time increased linearly, whereas the idle and rumination times decreased linearly with addition of castor meal. Feeding time, expressed in min per kg of DM and NDF, was not influenced by the inclusion of the meal in the diet, averaging 373.3 and 880.0 min, respectively. The number of chews and the time taken to ruminate each cud were not changed by inclusion of castor meal in the diet. Feed efficiency, expressed in grams DM and NDF per hour, was not influenced by castor meal inclusion in the diet. Rumination efficiency expressed in g DM h?1 decreased, but was not affected when expressed in g NDF h?1. Total replacement of soybean meal by castor meal in the concentrate does not affect the feeding time or feed efficiency of feedlot sheep, but leads to a small reduction of their rumination efficiency. Thus, we recommend the inclusion of castor meal in sheep diets at the levels tested in this study.

scholarly journals Use of sugar cane to feed lactating dairy goats

2020 ◽  
Vol 72 (6) ◽  
pp. 2297-2307
Author(s):  
A.M.D. Cabral ◽  
F.F.R. Carvalho ◽  
G.C.L. Santos ◽  
J.C. Ferreira ◽  
M.J.M.S. Silva ◽  
...  
Keyword(s):  
Milk Yield ◽  
Sugar Cane ◽  
Dry Matter ◽  
Latin Square ◽  
Neutral Detergent Fiber ◽  
Ingestive Behavior ◽  
Feeding Time ◽  
Critical Periods ◽  
Dairy Goats ◽  
The Difference

ABSTRACT The aim of the present study was to assess the effects of replacing corn silage with sugarcane in the diet of lactating Saanen goats and to determine their intake and digestibility of nutrients, ingestive behavior, milk yield and composition. The experimental diets were composed of increasing levels (0, 33, 66 and 100%) of substitution in dry matter (DM). Twelve multiparous Saanen goats, with an average body weight of 45.2kg, average milk yield of 3.0kg day-1, distributed in a triple latin square experimental design (4 × 4) were used. The dry matter intake (DMI) and other nutrients were estimated through the difference between the total nutrient in the food offered and its total in the leftovers. The DMI, crude protein, neutral detergent fiber (NDF) and total digestible nutrients were not influenced, but the apparent digestibility of DM and NDF decreased. Feeding time and feeding efficiency were not influenced, the rumination and total chewing times increased, and the leisure time decreased, both linearly. Milk yield was not influenced by substitution levels, but corrected milk yield to 3.5% fat decreased. Sugar cane represents a dietary alternative for goats with medium milk yield in critical periods of forage, since it does not change the consumption of DM and milk yield, even with the apparent declining digestibility of some nutrients, influencing the ingestive behavior of the animals.

scholarly journals Feeding behavior of dairy cows fed different levels of xiquexique (Pilosocereus gounellei)

2016 ◽  
Vol 38 (4) ◽  
pp. 417 ◽  
Author(s):  
Rafael Nogueira Furtado ◽  
Maria Socorro de Souza Carneiro ◽  
Elzânia Sales Pereira ◽  
Emilson Costa Moreira Filho ◽  
Eranildo Brasil da Silva
Keyword(s):  
Feeding Behavior ◽  
Dairy Cows ◽  
Latin Square ◽  
Experimental Period ◽  
Linear Increase ◽  
Lactating Dairy Cows ◽  
Behavioral Variables ◽  
The Times ◽  
Different Levels ◽  
Average Body

This study evaluated the replacement of Tifton hay with xiquexique (0, 12, 24 and 36%) on feeding behavior of lactating dairy cows. To this end, eight cows with average milk production of 15 kg milk day-1 and average body weight of 465.2 ± 39.4 kg were distributed in a double 4x4 Latin square. Each experimental period lasted 16 days, ten for adaptation and six for data collection. The record of behavioral variables was made every five min. for 24 hours. The times spent in feeding, ruminating and total chewing, expressed in hours day-1, as well as feeding (FE) and rumination (RE) efficiencies, expressed in g NDF h-1, the number of cuds and cud chews per day showed a linear decrease according to the levels of xiquexique in the diet. There was a linear increase in time in idleness. Xiquexique levels in the diet for dairy cows did not influence FE and RE, expressed in g DM h-1, number and time spent in chewing per cud. The replacement of Tifton hay with xiquexique in the diet alters the feeding behavior of dairy cows due to the reduction in fiber content. 

scholarly journals Use of docosahexaenoic acid for lamb diet

2020 ◽  
Vol 12 ◽  
pp. 1-8
Author(s):  
Maria Júlia Pereira Araújo ◽  
Erica Beatriz Schultz ◽  
Thauane Ariel Valadares Jesus ◽  
Marina Elizabeth Barbosa Andrade ◽  
Luciana Melo Sousa ◽  
...  
Keyword(s):  
Metabolic Profile ◽  
Dry Matter ◽  
Low Density Lipoprotein ◽  
Latin Square ◽  
Density Lipoprotein ◽  
Experimental Period ◽  
Corn Silage ◽  
Ingestive Behavior ◽  
Feed Water ◽  
Animal Category

The purpose of this study was to evaluate the effects of docosahexaenoic in the concentrated acid on intake, digestibility, metabolic profile and ingestive behavior of lambs diets. Five crossbred Dorper x Santa Inês lambs with initial age and body weight average of six months and 33 kg, respectively, were used in the trial. The animals were assigned into a 5×5 Latin square design and they received one of the following treatments: 0, 1.5, 3, 4.5 and 6 % of microalgal product (Aurantiochytrium limacinum algae) or ALL-G Rich® in the concentrate. The diets were composed of corn silage and concentrate offered twice daily. During the experimental period, feed, water, feces, blood, and urine were sampled to calculate the intake and digestibility and to characterize the metabolic profile. The ingestive behavior was measured during 24 hours on the last day each experimental period. The time spent on feeding, ruminating and idle activities was measured. Regression analyses were performed considering 5 % of significance. There were no difference on nutrients intake, digestibility, and ingestive behavior with the inclusion of ALL-G Rich® in the diet (p>0.05). There was a significant effect on serum concentrations of cholesterol, low density lipoprotein, alkaline phosphatase and gammaglutamyl transferase, however, all metabolites remained within the range recommended for the animal category. Thus, ALL-G Rich® can be used up to 6% in the concentrate, on dry matter basis, of lambs diet without affect intake, digestibility, metabolic profile, and ingestive behavior.

Feeding time and sequence of forage rape and maize silage does not affect digestibility and rumen parameters in sheep

2009 ◽  
Vol 49 (4) ◽  
pp. 318 ◽  
Author(s):  
R. Kaur ◽  
S. C. Garcia ◽  
W. J. Fulkerson
Keyword(s):  
Rumen Fluid ◽  
Latin Square ◽  
Sampling Period ◽  
Experimental Period ◽  
Ammonia Concentration ◽  
Feeding Time ◽  
Adaptation Period ◽  
Maize Silage ◽  
Forage Rape

The objective of this study was to evaluate the effect of time and sequence of feeding forage rape in relation to maize silage on whole tract digestibility and rumen parameters. Three rumen-fistulated castrated male sheep (45 ± 1.2 kg) were individually housed in metabolic crates and fed a diet comprising 25% forage rape, 25% maize silage, 10% concentrate and 40% short rotation ryegrass. The experimental design was a 3 × 3 Latin-square design and treatments were: forage rape fed after maize silage in the morning; forage rape fed before maize silage in the morning; and forage rape fed after maize silage in the afternoon. As the three treatments were identical in composition, the first experimental period comprised a 15-day adaptation period whereas the other two periods had a 7-day adaptation, each followed by 6-day sampling periods for measurement of feed intake and faeces and urine output (day 1–5). Rumen fluid was collected at day 6 of each sampling period and analysed for pH and ammonia concentration. In a subsequent experiment in sacco rumen degradation characteristics of all feeds were measured by incubating nylon bags in the rumen of each sheep. Neither dry matter (DM) intake (mean = 21.2 g/kg liveweight.day) nor whole tract in vivo DM (mean = 0.74) and neutral detergent fibre digestibility coefficients (mean = 0.69) were different (P > 0.05) among treatments. Similarly, neither rumen pH (6.19 ± 0.2) nor ammonia levels (27.2 ± 4.5 mg/100 mL) were affected (P > 0.05) by time of feeding forage rape in relation to maize silage. The calculated efficiency of microbial protein synthesis was similar (P = 0.73) for all treatments [mean = 17 g/day of microbial nitrogen (N) per kg of apparently digested organic matter in the rumen], although the excretion of urinary N was relatively high for all treatments due to high N intake and high degradation of N in forage rape. In conclusion, this study has revealed no effects of time and sequence of feeding forage rape with maize silage on rumen parameters and efficiency of feed utilisation. These results suggest that farmers using these forages can allocate these to animals based on practical convenience.

scholarly journals Intake, performance, and feeding behavior of Holstein and Holstein x Gyr heifers grazing intensive managed tropical grasses during the rainy season

2021 ◽  
Author(s):  
Daiana F Quirino ◽  
Marcos I Marcondes ◽  
Luciana N Renno ◽  
Camila S Cunha ◽  
Alex L Silva ◽  
...  
Keyword(s):  
Feeding Behavior ◽  
Dry Matter ◽  
Average Daily Gain ◽  
Fiber Material ◽  
Experimental Period ◽  
Nutrient Digestibility ◽  
Neutral Detergent Fiber ◽  
Ingestive Behavior ◽  
Panicum Maximum ◽  
And Performance

Holstein × Gyr and Holstein are the primary dairy breed used in tropical systems, but when rearing under pasture, feed intake, behavior, and performance might differ between them. This study aimed to evaluate the voluntary intake, nutrient digestibility, performance, and ingestive behavior of Holstein and Holstein × Gyr (½ Holstein × ½ Gyr) heifers managed in an intermittent grazing system of Guinea grass (Panicum maximum Jacq. cv. Mombaca). The experiment was conducted during the summer season throughout four periods of 21 d. Two 8-heifers (four Holstein and four Holstein × Gyr) groups, averaging 258.6 ± 24.8 kg and 157.1 ± 24.99 kg body weight, were used. Each group grazed a separate set of 16 paddocks, and all heifers received a concentrate supplement daily. Heifers were weighed at the beginning and end of the experiment. Fecal, forage and concentrate samples were evaluated for their dry matter (DM), crude protein (CP), crude fat, ash, neutral detergent fiber (NDF), and indigestible NDF. Feeding behavior was evaluated through 24 h of live observation for 48 h of each experimental period. Grazing, ruminating, resting, and intake of concentrate times were recorded, and rumination criteria, bout criteria, mealtime, meal frequency, and meal duration were estimated. There was no difference in dry matter intake (DMI). The Holstein × Gyr heifers had greater NDF intake and average daily gain (ADG), and feed efficiency tended to show greater CP and NDF digestibilities. The forage DMI of Holstein × Gyr was 11.70% greater than the Holstein heifers. Holstein grazed less than Holstein × Gyr heifers in the afternoon. Ruminating time was 18.43% lower for Holstein than Holstein × Gyr heifers, and rumination criteria were greater for Holstein heifers. Holstein heifers presented more prolonged rumination bouts and resting time than Holstein × Gyr heifers. Holstein × Gyr can ingest and ruminate greater amounts of fibrous material. Holstein heifers select lower fiber material, and they need to spend more time ruminating small portions of feed. Overall, we do not recommend using young Holstein heifers in tropical pasture conditions because their ADG is low because of its lower adaptability to fibrous feed and heat stress. However, this management condition is appropriate for Holstein × Gyr heifers and results in an adequate performance.

scholarly journals Intake, digestibility, and performance of lambs fed spineless cactus cv. Orelha de Elefante Mexicana

2020 ◽  
Vol 33 (8) ◽  
pp. 1284-1291
Author(s):  
Levi Auto Lopes ◽  
Marcelo de Andrade Ferreira ◽  
Ângela Maria Vieira Batista ◽  
Michel do Vale Maciel ◽  
Rodrigo Barbosa de Andrade ◽  
...  
Keyword(s):  
Experimental Period ◽  
Neutral Detergent Fiber ◽  
Ingestive Behavior ◽  
Average Weight ◽  
Feeding Time ◽  
Randomized Design ◽  
Significant Difference ◽  
Ultrasound Measurements ◽  
Total Carbohydrates ◽  
And Performance

Objective: To evaluate the effects of the carmine cochineal-resistant spineless cactus genotypes cv. Orelha de Elefante Mexicana (<i>Opuntia</i>) and Miúda (<i>Nopalea</i>) on the intake and digestibility of nutrients, ingestive behavior, performance, and ultrasound measurements of growing lambs.Methods: Thirty-six male (non-castrated) Santa Inês lambs were used, with an average age of 6 months and an initial average weight of 22.0±2.9 kg. They were distributed in a completely randomized design with 3 treatments (Tifton hay, <i>Nopalea</i> and <i>Opuntia</i>) and 12 replications, using initial weight as a covariate. The experimental period was 86 days, with the first 30 days used for the adaptation of the animals to the facilities, diets and management, and the remaining 56 days used for evaluation and data collection.Results: The intake and apparent digestibility of dry matter (DM), organic matter (OM), crude protein (CP), neutral detergent fiber (NDF), total carbohydrates (TC), non-fibrous carbohydrates (NFC), and total digestible nutrients (TDN) showed a significant difference (p<0.05) as a function of the diets, with the <i>Nopalea</i> treatment (p<0.05) increasing DM intake (g/kg and % body weight [BW]), CP, TDN, and TC digestibility, whereas the Tifton hay diet led to a high (p<0.001) neutral detergent fiber corrected for ash and protein (NDFap) g/d intake, NDFap (BW %) and digestibility of said nutrient. There was no effect of treatments (p>0.05) on feeding time, however, rumination time and total chewing time were higher (p<0.05) for animals fed Tifton hay. The performance of the animals was similar (p>0.05). For the ultrasound measurements, <i>Nopalea</i> promoted an increase in the final loin eye area, compared to Tifton hay.Conclusion: The use of spineless cactus variety Miúda leads to the greater intake and digestibility of nutrients. The evaluated carmine cochineal-resistant spineless cactus genotypes are alternatives for semi-arid regions as they do not negatively affect the performance of growing lambs.

scholarly journals Trehalose and glucose levels regulate feeding behavior of the phloem-feeding insect, the pea aphid Acyrthosiphon pisum Harris

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Guang Wang ◽  
Jing-Jiang Zhou ◽  
Yan Li ◽  
Yuping Gou ◽  
Peter Quandahor ◽  
...  
Keyword(s):  
Feeding Behavior ◽  
Growth And Development ◽  
Acyrthosiphon Pisum ◽  
Pea Aphid ◽  
Feeding Time ◽  
Electrical Penetration Graph ◽  
Glucose Content ◽  
Glucose Levels ◽  
Phloem Feeding ◽  
Trehalose Content

AbstractTrehalose serves multifarious roles in growth and development of insects. In this study, we demonstrated that the high trehalose diet increased the glucose content, and high glucose diet increased the glucose content but decreased the trehalose content of Acyrthosiphon pisum. RNA interference (RNAi) of trehalose-6-phosphate synthase gene (ApTPS) decreased while RNAi of trehalase gene (ApTRE) increased the trehalose and glucose contents. In the electrical penetration graph experiment, RNAi of ApTPS increased the percentage of E2 waveform and decreased the percentage of F and G waveforms. The high trehalose and glucose diets increased the percentage of E2 waveform of A. pisum red biotype. The correlation between feeding behavior and sugar contents indicated that the percentage of E1 and E2 waveforms were increased but np, C, F and G waveforms were decreased in low trehalose and glucose contents. The percentage of np, E1 and E2 waveforms were reduced but C, F and G waveforms were elevated in high trehalose and glucose contents. The results suggest that the A. pisum with high trehalose and glucose contents spent less feeding time during non-probing phase and phloem feeding phase, but had an increased feeding time during probing phase, stylet work phase and xylem feeding phase.

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