scholarly journals PSX-26 Levels of forage and narasin inclusion on rumen fermentation in feedlot lambs

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 456-457
Author(s):  
Lairana A Sardinha ◽  
Daniel M Polizel ◽  
Alexandre A Miszura ◽  
Arnaldo C Limede ◽  
José P R Barroso ◽  
...  

Abstract The objective of this study was to determine the effects of forage levels and narasin inclusion in diets on the rumen parameters in feedlot lambs. Forty-four lambs were allotted in a randomized complete block design, defined by initial body weight, in a 2x2 factorial arrangement. The first factor was forage inclusion (10 or 20% of coastcross hay, DM basis) and the second factor was narasin (0 or 13 ppm). The experimental diets were isonitrogenous (17.4%, DM basis). At the end of the experimental period, the lambs were slaughtered without fasting, and the rumen fluid was collected to determinate pH and short chain fatty acid (SCFA). Data were analyzed using the MIXED procedure of SAS and the LSMEANS option was used to obtain the means. The effects were considered significant when P < 0.05. There was an interaction between hay and narasin on the molar proportion of propionate (P = 0.02) and the acetate:propionate ratio (P < 0.01). Narasin inclusion in diets containing 20% forage increased propionate (25.4 vs 37.1 mM/100mM; P = 0.02); however, there was no effect in diets containing 10% forage (35.9 ± 1.75 mM/100Mm). Consequently, the narasin decreased acetate:propionate ratio in diets containing 20% forage (2.57 vs 1.42; P < 0.01), and no effect was observed 10% forage diets (1.40 ± 0.14). The inclusion of narasin increased the total SCFA (86.2 vs 115.4 mM; P < 0.01), ruminal pH (6.26 vs 6.49; P = 0.05) and decreased acetate (55.6 vs 47.6 mM/100mM; P < 0.01). The inclusion of 20% forage increased acetate when compared with 10% hay (54.99 vs 48.19 mM/100mM; P < 0.01) and the pH ruminal (6.25 vs 6.49; P = 0.04). The experimental diets did not affect the butyrate. In summary, the narasin inclusion and forage levels content change rumen parameters in feedlot lambs.

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 456-456
Author(s):  
Lairana A Sardinha ◽  
Daniel M Polizel ◽  
Marcelo Baggio ◽  
Arnaldo C Limede ◽  
José P R Barroso ◽  
...  

Abstract Narasin is an antibiotic ionophore that may change rumen fermentation; however, the effects on the post-rumen are unknown. The objective of this study was to determinate the effects of different levels of hay and narasin inclusion on the cecal parameters of lambs. Forty-four lambs were allotted in a randomized complete block design, defined by initial BW, in a 2x2 factorial treatment arrangement. The first factor was forage (coastcross hay) inclusion (10 or 20%, DM basis) and the second factor was narasin (0 or 13 ppm). The experimental diets were isonitrogenous (17.4%, DM basis) and the experiment lasted 112 days. At the end of the experimental period, the lambs were slaughtered without fasting, and the cecal fluid was collected to determinate pH and short chain fatty acid (SCFA). Data were analyzed using the MIXED procedure of SAS. The effects were considered significant when P < 0.05. There was no interaction between forage content and narasin inclusion. Narasin increased the molar concentration of propionate (N0: 19.4; N13: 21.4 mM/100mM; P = 0.02) and decreased isobutyrate (N0: 1.6; N13: 0.7 mM/100mM; P = 0.05) and acetate:propionate ratio (N0: 3.4; N13: 2.9; P = 0.03). The valerate was greater in diets with 10% hay when compared of diets 20% (H10: 2.0; H20: 1.3 mM/100mM; P < 0.01). The experimental diets not affect the molar proportion of acetate (62.7±1.3 mM/100mM), butyrate (12.9 ± 0.7 mM/100mM), isovalerate (1.3 ± 0.5 mM/100mM) total SCFA concentration (68.6 ± 8.5 mM) and cecal pH (6.2 ± 0.14). Therefore, the inclusion of 13 ppm of narasin changes the cecal fermentation in high concentrate diets. In addition, the inclusion of 10 or 20% of hay has a little influence on cecal fermentation.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 424-425
Author(s):  
Evandro M Ferreira ◽  
Nathalia R Eckermann ◽  
Janaina S Biava ◽  
Matheus Avelino ◽  
Letícia Pasqualino ◽  
...  

Abstract Fifty-eight Dorper x Santa Inês ram lambs (initial BW 20.7 ± 1.19 kg and 75 ± 10.83 d old) were assigned to a randomized complete block design to evaluate the effects of fiber content on ruminal and cecal pH and SCFA. The treatments were defined by the fiber (“Coastcross” hay) content in diets (CP: 16,12% ± 0.92): 0F: no forage diet (NDF: 10.4%); 5F: 5% of forage (NDF: 12.1%); 10F: 10% of forage (NDF: 15%); 15F: 15% of forage (NDF: 15.5%); 20F: 20% of forage (NDF: 20.8%) and 25F: 25% of forage (NDF: 23.5%). The experiment lasted 89 days, at the end of the experiment the lambs were slaughtered without fasting. The forage inclusion linear decreased ruminal concentration of propionate (0F: 45.4; 5F: 39.7; 10F: 41.5; 15F: 39.4; 20F: 35.3; 25F: 30.5 mM/100mM; P < 0.01) and total SCFA (0F: 106.6; 5F: 96.4; 10F: 84.2; 15F: 90.6; 20F: 63.0; 25F: 71.2 mM; P = 0.01) and increased the ruminal concentration of acetate (0F: 43.4; 5F: 45.3; 10F: 47.8; 15F: 49.8: 20F: 51.8; 25F: 57.2 mM/100mM; P < 0.01). However, there was a quadratic effect on ruminal pH (0F: 5.61; 5F: 5.66; 10F: 5.98; 15F: 6.27; 20F: 6.05; 25F: 5.74; P = 0.01). There was no effect of fiber levels on cecal concentration of acetate, propionate, butyrate and acetate:propionate ratio. Nevertheless, there was a linear decrease in cecal concentration of valerate (0F: 3.28; 5F: 2.35; 10F: 2.39; 15F: 2.64: 20F: 2.12; 25F: 1.80 mM/100mM; P < 0.01) and total SCFA (0F: 173.9; 5F: 165.7; 10F: 153.2; 15F: 155.2; 20F: 139.9; 25F: 128.9 mM; P < 0.01). Similarly to rumen, cecal pH presented a quadratic response (0F: 5.92; 5F: 6.12; 10F: 6.15; 15F: 6.23; 20F: 6.30; 25F: 5.83; P < 0.01). In conclusion, cecal fermentation was different from rumen fermentation. However, pH variation was similar, with higher values for 15F diet.


2018 ◽  
Vol 31 (1) ◽  
pp. 1-8
Author(s):  
MARCOS DA SILVA BRUM ◽  
THOMAS NEWTON MARTIN ◽  
VINÍCIUS SANTOS DA CUNHA ◽  
LUIZ FERNANDO TELEKEN GRANDO ◽  
ALEX TAGLIAPIETRA SCHONELL

ABSTRACT The objective of this study was to evaluate the phytomorphological parameters of soybean in a crop-livestock system inoculated with Azospirillum brasilense. The experiment was conducted in Santa Maria, Brazil, for two agricultural years (2 Paper extracted from the doctoral thesis of the first author. 012/2013 and 2013/2014) in a randomized complete block design with three replications. In the winter, the black oat and ryegrass pasture was managed with sheep under different grazing systems: (I) - a conventional grazing (CG) system, where the animals remained in the pasture throughout the experimental period without any pasture height control; (II, III, and IV) - systems with post-grazing pasture heights of 10, 20, and 30 cm, respectively; and (NG) - one control without grazing. All treatments received two nitrogen doses (50 and 100 kg ha-1) and inoculation or no inoculation (A. brasilense). The soybean cultivar BMX Potência RR was sown using no-tillage on 16 November 2012 and 1 December 2013. In each plot, seven rows of plants with spacing of 0.45 m were used, and in four rows, the seeds were inoculated with the bacterium A. brasilense. The phytomorphological variables and grain productivity were evaluated. In a croplivestock system, soybean has better productivity when established on black oat and ryegrass pasture managed with post-grazing pasture heights of between 20 and 30 cm. Areas under conventional grazing in the winter led to smaller soybean plants with lower first and last pod heights. Inoculation with A. brasilense should not be used alone in soybean cultivation.


2017 ◽  
Vol 69 (4) ◽  
pp. 1039-1046 ◽  
Author(s):  
B.C. Pedreira ◽  
P.L. Barbosa ◽  
L.E.T. Pereira ◽  
M.A. Mombach ◽  
L.F. Domiciano ◽  
...  

ABSTRACT The objective of this study is to verify the population density and the dynamics of tillering in the Marandu palisade grass sward subjected to nitrogen (N) fertilization strategies, characterized by the N supply via urea or bacterial inoculant (Azospirillum brasilense). The treatments comprised of four nitrogen fertilization strategies: (A) Without fertilization, (B) 80 kg N/ha, (C) inoculant (A. brasilense), and (D) 80 kg N/ha + inoculant, distributed in a randomized complete block design, with three replications. The nitrogen supply strategies were evaluated during six periods: October, November, and December (2012) as well as January, March, and April (2013). The nitrogen dose or inoculant had no effect on the tiller appearance rate (TAR), tiller mortality rate (TMR), tiller survival rate (TSR), or tiller population density (TPD). However, these variables were influenced by the season. The TAR and TSR were higher at the beginning of the experimental period (October) and lower towards the end of the period (March-April), whereas, TMR and TPD exhibited the opposite behavior, with lower values in October and higher from January onward. Neither the nitrogen nor the inoculant influenced the population dynamics of the tillers in Marandu palisade grass.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 455-455
Author(s):  
Lairana A Sardinha ◽  
Daniel M Polizel ◽  
Alexandre A Miszura ◽  
Arnaldo C Limede ◽  
José P R Barroso ◽  
...  

Abstract The objective of this study was to determinate the effects of narasin on carcass characteristics of lambs fed a diet containing different levels. Forty-four lambs were allotted in a randomized complete block design, defined by initial body weight (BW), in a 2x2 factorial arrangement. The first factor was forage (coastcross hay) inclusion (10 or 20%, DM basis) and the second factor was narasin (0 or 13 ppm). The experimental diets were isonitrogenous (17.4%, DM basis). The experiment lasted 112 days. At the end of the experiment, the lambs were slaughtered to evaluate the carcass characteristics and meat composition. Data were analyzed using the MIXED procedure of SAS and the LSMEANS option was used to obtain the means. The effects were considered significant when P < 0.05. There was no interaction between forage levels and narasin inclusion. The experimental diets did not affect the slaughter BW (54.8 ± 1.6 kg), hot carcass weight (29.3 ± 0.96 kg), dressing percentage (53.5 ± 0.67%) and LM area (18.8 ± 0.89 cm2). The inclusion of narasin increased 12th-rib fat (2.10 vs 2.75 mm; P < 0.01) and BW thickness (16.9 vs 20.2 mm; P < 0.01). The inclusion of 20% of hay decreased BW thickness (19.21 vs 17.9 mm; P = 0.03). The experimental diets did not affect the moisture (74.2 ± 0.53%) and ash content in meat (1.27 ± 0.04%). Narasin inclusion increased the fat (2.81 vs 3.62%; P < 0.01) and decreased the CP (21.3 vs 20.7%; P = 0.02) in meat composition. The inclusion of 10% of hay increased the fat in meat (3.5 vs 2.9%; P = 0.03), however, did not affect the CP content. In conclusion, the narasin inclusion and levels of hay improve the carcass characteristics and change the meat composition.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 424-424
Author(s):  
Evandro M Ferreira ◽  
Nathalia R Eckermann ◽  
Janaina S Biava ◽  
Jamile Comelli ◽  
Thamires U Sturion ◽  
...  

Abstract High-grain diets have been used with great success in the feedlot systems for lambs production in Brazil. Then it became important to define the ideal dietary fiber content in the diet when the goal is to produce heavy lambs for early slaughter. The objective in this trial was to evaluate the levels of fiber on performance of lambs feed high-grain diet. Fifty-eight Dorper x Santa Inês ram lambs (initial BW 20.7 ± 1.19 kg and 75 ± 10.83 d old) were assigned to a randomized complete block design. The treatments were defined by the fiber (“Coastcross” hay) content in diets (CP: 16,12% ± 0.92): 0F: no forage diet (NDF: 10.4%); 5F: 5% of forage (NDF: 12.1%); 10F: 10% of forage (NDF: 15%); 15F: 15% of forage (NDF: 15.5%); 20F: 20% of forage (NDF: 20.8%) and 25F: 25% of forage (NDF: 23.5%). The experiment lasted 89 days and lambs were weighted after 16 hours fasting on days 0, 31, 57 and 89. DMI, ADG and FE were determined in each period. There was no interaction effect between diets and periods. There was a quadratic effect in DMI (0F: 0.96; 5F: 0.97; 10F: 1.04; 15F: 1.00; 20F: 1.02; 25F: 0.88 kg/d; P = 0.02), ADG (0F: 0.27; 5F: 0.26; 10F: 0.28; 15F: 0.28; 20F: 0.26; 25F: 0.21 kg/d; P = 0.01) and FE (0F: 0.28; 5F: 0.28; 10F: 0.28; 15F: 0.29; 20F: 0.26; 25F: 0.25; P = 0.05). Consequently, the increased levels of forage result in quadratic effect on final BW (0F: 44.5; 5F: 44.7; 10F: 46.1; 15F: 45.5; 20F: 43.9; 25F: 39.7 kg; P < 0.01). In conclusion, the inclusion of 10–15% of forage improves the performance of feedlot lambs feed high-concentrate diets, but it was proved the real possibility of finishing lambs with diet without forage source.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 71-71
Author(s):  
Nicole T Briggs ◽  
Bayissa Hatew ◽  
Michael A Steele

Abstract Inorganic sources of trace minerals are commonly supplemented in dairy cow diets; however, there has been an increase in the supplementation of minerals complexed with organic compounds. These organic trace minerals are thought to have greater bioavailability which may enhance rumen fermentation and absorption. The objective of this study was to assess the effects of dietary concentration and source of supplemental trace minerals on serum trace mineral status and rumen fermentation. Six lactating Holstein cows were used in a 6 x 6 Latin square design with a 23-day adaptation and 5-day experimental period. Cows were fed the same basal diet daily except for the difference in source [organic (ORG) versus inorganic (INO)] and concentration (50%, 100%, and 200% based on NRC recommendations) of trace mineral supplemented. During the experimental period feed intake and blood were collected daily. Rumen fluid was collected on the final two days of the experimental period. Data was analyzed with PROC MIXED in SAS 9.4. Dry matter intake (18.1 ± 0.70 kg), serum mineral concentrations (Cu, Mn, Se, and Zn), and rumen pH (6.5 ± 0.64) did not differ among the treatments. However, serum concentration of Co was higher in 200% ORG compared to 50% and 100% INO and 50% ORG. Ruminal concentration of acetate was higher in 50% and 100% ORG compared to 200% ORG. Butyrate ruminal concentration was higher at 50% ORG compared to 200% ORG. Ruminal propionate concentration was higher in 50% INO and 50% ORG compared to 100% INO and 200% ORG. These findings demonstrate serum trace mineral status and ruminal pH are not tightly controlled by the source of trace minerals when supplemented at 50%, 100% and 200% of the NRC recommendations, however rumen fermentation may be affected by the dietary concentration of trace minerals in the diet.


Author(s):  
Danung Nur Adli ◽  
Osfar Sjofjan

The aim of this study was to assess the effects of dietary probiotic enhanced liquid acidifier combined with mannan-rich fraction on growth performance, nutrients digestibility in growing pigs. Thirty [(Duroc×Yorkshire)×Landrace] pigs with the average initial BW of 36.75±1.57 kg were allocated into three treatments by a randomized complete block design. There were five pens per treatment with six pigs per pen. Dietary treatments include: 1) CON (basal diet); 2) T1 (basal diet+probiotic 0.1%) and 3) T2 (basal diet+probiotic+mannan rich fraction 0.2%). the data were analyzed as a randomized complete block design using of SAS University Version 4.0. The model included the effects of block (replication) and treatment. Pen served as the experimental unit. During the entire experimental period of 6 weeks, results showed that addition of complex probiotic at the level of 0.2% to diet increased ADG significantly (p<0.05). Also, digestibility of DM and N tended to increase. To sum up, results in this experiment indicated that dietary [(probiotik×acidifier)×mannan-rich-fraction]  supplementation had a positive effect on growing pigs performance and nutrient digestibility


Author(s):  
Luiz Guilherme Mezzena Gobato ◽  
Daniel Montanher Polizel ◽  
Marcos Vinícius de Castro Ferraz Júnior ◽  
Alexandre Arantes Miszura ◽  
José Paulo Roman Barroso ◽  
...  

Abstract: The objective of this work was to evaluate the effect of narasin inclusion in mineral and protein supplements on the performance and supplement intake of grazing beef yearlings. One hundred and fifty Nellore yearlings with 17.0±0.06 months of age and 219.7±1.62 kg initial body weight (BW) were allocated into 30 experimental units (EUs), with five animals each. The EUs were assigned to 16 paddocks, which were continuously stocked for 28 days, followed by 28 days of rest. A randomized complete block design was used. The experiment lasted 140 days, divided into five periods of 28 days each. The assessed treatments were: control mineral supplement (CON); CON + 1,800 (N1800) or 2,750 (N2750) mg narasin per kilogram of supplement; and protein mineral supplement (PROT) or PROT + 360 mg narasin per kilogram of supplement (PROT 360). The inclusion of narasin in the mineral supplement increased average daily gain (ADG) in the first period and decreased supplement intake in the first, second, and third periods, when compared with the CON treatment. The ADG and supplement intake were similar between the N1800 and N2750 treatments. Narasin has a positive effect on ADG up to 28 days of supplementation and controls mineral supplement intake, without compromising this gain.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 422-423
Author(s):  
Janaina S Biava ◽  
Nathalia R Eckermann ◽  
Thamires U Sturion ◽  
Daniel M Polizel ◽  
Paulo Dias Junior ◽  
...  

Abstract The objectives of this trial were to evaluate the effects of levels of fiber in high-grain diets on carcass characteristics of feedlot lambs. Fifty-eight Dorper x Santa Inês ram lambs (initial BW 20.7 ± 1.19 kg and 75 ± 10.83 d old) were assigned to a randomized complete block design. The treatments were defined by the fiber (“Coastcross” hay) content in the diets (CP: 16,12% ± 0.92): 0F: no forage diet (NDF: 10.4%); 5F: 5% of forage (NDF: 12.1%); 10F: 10% of forage (NDF: 15.0%); 15F: 15% of forage (NDF: 15.5%); 20F: 20% of forage (NDF: 20.8%) and 25F: 25% of forage (NDF: 23.5%). The experiment lasted 89 days, at the end of this time, the animals were slaughtered (169 ± 10.83 d old) to evaluate carcass characteristics. There was a quadratic response for slaughter BW (0F: 46.3; 5F: 48.1; 10F: 49.4; 15F: 50.3; 20F: 48.5; 25F: 43.3 kg; P &lt; 0.01), hot carcass weight (0F: 24.8; 5F: 25.6; 10F: 26.0; 15F: 25.7; 20F: 24.3; 25F: 21.8 kg; P = 0.01), cold carcass weight (0F: 24.3; 5F: 25.1; 10F: 25.4; 15F: 25.1; 20F: 23.8; 25F: 21.2 kg; P = 0.01) and longissimus dorsis area (0F: 16.3; 5F: 16.5; 10F: 17.4; 15F: 16.5; 20F: 16.0; 25F: 15.1 cm2; P = 0.04). There was a linear decrease in the hot carcass yield (0F: 53.0; 5F: 53.3; 10F: 52.5; 15F: 50.9; 20F: 50.1; 25F: 50.2%; P &lt; 0.01), cold carcass yield (0F: 52.0; 5F: 52.2; 10F: 51.4; 15F: 49.8; 20F: 49.0; 25F: 48.9%; P &lt; 0.01) and body wall thickness (0F: 16.2; 5F: 17.0; 10F: 16.3; 15F: 16.3; 20F: 15.1; 25F: 13.6 kg; P = 0.01). There was no effect on subcutaneous fat thickness. In conclusion, the diet with up to 5% of forage allowed higher carcass yield; however, the heavier carcasses were produced by the lambs that received 10F diet.


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