scholarly journals PSXIII-23 Dietary glutamine, glutamate, and aspartate supplementation improves morphology and intercellular junction of small intestine in piglets

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 472-474
Author(s):  
Jing Wang ◽  
Bi E Tan ◽  
Jianjun Li ◽  
Ming Qi ◽  
Wenkai Ren ◽  
...  
Keyword(s):  
Small Intestine ◽  
Basal Diet ◽  
Low Energy ◽  
Intestinal Morphology ◽  
Control Group ◽  
Mrna Levels ◽  
Villus Height ◽  
Final Body Weight ◽  
Weaning Stress ◽  
Low Energy Diet

Abstract Weaning-stress decreases the digestive and absorptive capacity of small intestine in piglets, resulting in reduction in energy intake for intestine cells and defects in epithelial structure. As glutamine (Gln), glutamate (Glu), and aspartate (Asp) are major energy sources for small intestine; thus, this study was conducted to test the hypotheses that supplementation with Gln, Glu, and Asp in diets will improve the intestinal morphology and tight junction in weaning piglets. 198 weaned piglets were assigned to the following treatments: i) Control (Basal diet + 1.59% L-Alanine); ii) T1 (Basal diet + 1% L-Glutamine + 0.5% L-Glutamate + 0.1% L-Aspartate); iii) T2 (Low energy diet + 1% L-Glutamine + 0.5% L-Glutamate + 0.1% L-Aspartate). The small intestinal samples were obtained on 5 or 21-day-post-weaning. The results showed that basal dietary supplementation with Gln, Glu, and Asp in basal diet improved the final body weight (BW), average daily gain (ADG) of piglets on 21-day of post-weaning. Supplementation with Gln, Glu, and Asp in diet with low energy decreased the villus height and crypt depth in ileum of piglets on 5-day-post-weaning, but increased villus height and goblet cell number in jejunum on 21-day post-weaning compared with those in control group. On 5-day-post-weaning, high mRNA levels of voltage-gated potassium (Kv) 1.1 in ileum and Kv 1.5 in jejunum were observed in T 1 and T 2 groups, respectively. Other indicators were higher than those in control piglets on day 5 or 21 post-weaning, including protein abundances of claudin-1, laudin-3 and occluding, and the percentage of proliferating cell nuclear antigen (PCNA)-positive cells in jejunum and ileum in T 1 or T 2 groups. Collectively, these findings indicated that Gln, Glu, and Asp can alleviate the intestinal barrier injury in piglets induced by weaning stress even under low energy diet.

scholarly journals Glutamine, Glutamate, and Aspartate Improves Morphology and Energy Production of Small Intestine in Piglets with Different Energy Levels Diets

2021 ◽  
Author(s):  
Jing Wang ◽  
Nan Wang ◽  
Ming Qi ◽  
Jianjun Li ◽  
Bie Tan ◽  
...  
Keyword(s):  
Small Intestine ◽  
Energy Metabolism ◽  
Energy Intake ◽  
Energy Homeostasis ◽  
Basal Diet ◽  
High Energy ◽  
Low Energy ◽  
Intestinal Morphology ◽  
Small Intestinal ◽  
Low Energy Diet

Abstract Background: Weaning-stress may cause reduced energy intake for maintenance of mucosal structure. Glutamine (Gln), glutamate (Glu), and aspartate (Asp) are major energy sources for small intestine. This study investigated whether Gln, Glu, and Asp improve the intestinal morphology via regulating the energy metabolism in weaning-piglet. A total of 198 weaned-piglets were assigned to the following treatments: Control (Basal diet + 1.59% L-Alanine); T1 (Basal diet + 1% L-Gln + 0.5% L-Glu + 0.1% L-Asp); T2 (Low energy diet + 1% L-Gln + 0.5% L-Glu + 0.1% L-Asp). Jejunum and ileum were obtained on d-5 or d-21 post-weaning. Results: Improved growth performance on d-21 post-weaning were observed in T1 treatment. Both T1 and T2 treatments improved small intestinal morphology by increasing villus height, goblet cell number and decreasing crypt depth on d 5 or d-21 post-weaning. Gln, Glu, and Asp could restore the small intestinal energy homeostasis via replenishing the Krebs’ cycle and down-regulate the AMPK pathway. However, when piglets fed by a low energy diet, Gln, Glu, and Asp are not sufficient to maintain the intestinal energy balance on d-5 post-weaning so that the AMPK, beta-oxidation, and mitochondrial biogenesis are activated to meet the high energy demand of enterocytes. Conclusion: These data indicated that Gln, Glu, and Asp could restore the energy homeostasis of intestinal mucosa of piglets. And the mucosal energy metabolism showed different responses to the intervention of Gln, Glu, and Asp in piglets with a low energy diet between d5 and d21 post-weaning. These findings provide new information on the nutritional intervention for the insufficient energy intake in weaning-piglet.

scholarly journals PSVIII-18 Dietary supplementation with trihexanoin enhances intestinal function of weaned piglets

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 302-303
Author(s):  
Tao Wu
Keyword(s):  
Fat Metabolism ◽  
Dietary Supplementation ◽  
Basal Diet ◽  
Intestinal Morphology ◽  
Mucosal Barrier ◽  
Mrna Levels ◽  
Intestinal Function ◽  
Weaned Piglets ◽  
Villus Height ◽  
And Function

Abstract Trihexanoin is a short-chain triglyceride (SCT). Many studies have reported that SCTs play important roles in the maintenance of intestinal epithelial structure and function. The present work was to investigate the effects of trihexanoin on growth performance, carbohydrate and fat metabolism, as well as intestinal morphology and function in weaned piglets. Twenty weaned piglets (21 ± 2 d) were randomly allocated to one of two treatment groups: the control group (basal diet supplemented with 0.5% soya oil); the TH group (basal diet supplemented with 0.5% trihexanoin). Dietary trihexanoin supplementation significantly reduced diarrhea rate (P < 0.05); increased the concentrations of LDL, HDL and total protein, decreased cholesterol concentrations (CHOL) and glutamyl transpeptidase (GGT) activity in plasma (P < 0.05); increased villus height, surface area, and the ratio of villus height to crypt depth (P < 0.05); altered the mRNA levels and abundances of proteins related to glycogen and fat metabolism (gene LIPE, LPL, PPARG, ACACA, FASN, SLC27A2, INSR, PCK1 and ASS1), mucosal barrier function (protein claudin-1, and occludin), antioxidant capacity (protein HSP70 and gene Nrf2, NOX2 and GSTO2) and water transport capacity (protein AQP3 and gene AQP8 and AQP10) (P < 0.05); altered the gene abundance of intestinal bacteria (Enterobacteriaceae, Enterococcus, Clostridium, Lactobacillus and Bifidobacterium) (P < 0.05). In conclusion, dietary supplementation of trihexanoin improved the intestinal function and health of weaned piglets by regulating nutrient metabolism, improving intestinal function of mucosal barrier, transport, absorption and antioxidant, and altering the community of microbiota.

scholarly journals Dietary Supplementation With Bacillus subtilis Promotes Growth and Gut Health of Weaned Piglets

2021 ◽  
Vol 7 ◽  
Author(s):  
Zhilong Tian ◽  
Xiaodan Wang ◽  
Yehui Duan ◽  
Yue Zhao ◽  
Wenming Zhang ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Dietary Supplementation ◽  
Basal Diet ◽  
Intestinal Morphology ◽  
Control Group ◽  
Gut Health ◽  
Weaned Piglets ◽  
Villus Height ◽  
Expression Of Genes ◽  
Group A

This study was conducted to investigate the effects of dietary supplementation with different types of Bacillus subtilis (B. subtilis) on the growth and gut health of weaned piglets. A total of 160 piglets were randomly assigned into four groups: control group (a basal diet), BS-A group (a basal diet supplemented with B. subtilis A at 1 × 106 CFU/g feed), BS-B group (a basal diet supplemented with B. subtilis B at 1 × 106 CFU/g feed), and BS-C group (a basal diet supplemented with B. subtilis C at 1 × 106 CFU/g feed). All groups had five replicates with eight piglets per replicate. On days 7, 21, and 42 of the trial, blood plasma and intestinal tissues and digesta samples were collected to determine plasma cytokine concentrations, intestinal morphology, gut microbiota community and metabolic activity, and the expression of genes related to gut physiology and metabolism. The results showed that dietary B. subtilis supplementation improved (P < 0.05) the body weight and average daily gain (in BS-B and BS-C groups) of weaned piglets and decreased (P < 0.05) the diarrhea rates (in BS-A, BS-B, and BS-C groups). In the intestinal morphology analysis, B. subtilis supplementation improved (P < 0.05) the size of villus height and villus height to crypt depth ratio in the ileum of weaned piglets. Firmicutes, Bacteroidetes, and Tenericutes were the most dominant microflora in piglets' colon whatever the trial group and time of analysis. Dietary BS-C supplementation increased (P < 0.05) the relative abundances of Anaerovibrio and Bulleidia and decreased (P < 0.05) the relative abundances of Clostridium and Coprococcus compared with the control group. In addition, dietary B. subtilis supplementation increased (P < 0.05) the indicators of intestinal health, including plasma levels of interleukin (IL)-2 and IL-10, as well as the colonic levels of short-chain fatty acids. Furthermore, dietary B. subtilis supplementation also up-regulated (P < 0.05) the expression of genes involved in metabolic pathways related to intestinal microbiota maturation. In conclusion, these findings suggest that a diet containing BS-B or BS-C can efficiently promote growth performance, decrease diarrhea incidence, and ameliorate several indicators of intestinal health through the modulation of gut microbiota composition and metabolic activity in weaned piglets.

scholarly journals Effects of Xylo-Oligosaccharides on Growth and Gut Microbiota as Potential Replacements for Antibiotic in Weaning Piglets

2021 ◽  
Vol 12 ◽  
Author(s):  
Yuxia Chen ◽  
Yining Xie ◽  
Ruqing Zhong ◽  
Lei Liu ◽  
Changguang Lin ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Basal Diet ◽  
Intestinal Morphology ◽  
Short Chain ◽  
Control Group ◽  
Rrna Gene ◽  
Weaned Piglets ◽  
Villus Height ◽  
Crypt Depth ◽  
Weaning Piglets

Xylo-oligosaccharides (XOS) is a well-known kind of oligosaccharide and extensively applied as a prebiotic. The objective of this study was to investigate the effect of XOS supplementation substituting chlortetracycline (CTC) on growth, gut morphology, gut microbiota, and hindgut short chain fatty acid (SCFA) contents of weaning piglets. A total of 180 weaned piglets were randomly allocated to three treatments for 28 days, as follows: control group (basal diet, CON), basal diet with 500 mg/kg (XOS500) XOS, and positive control (basal diet with 100 mg/kg CTC). Compared with the CON group, the piglets in the XOS500 group improved body weight (BW) on days 28, average daily gain (ADG) and reduced feed: gain ratio during days 1–28 (P < 0.05). The XOS500 supplementation increased Villus height and Villus height: Crypt depth ratio in the ileum (P < 0.05). Villus Height: Crypt Depth of the ileum was also increased in the CTC treatment group (P < 0.05). Meanwhile, the XOS500 supplementation increased significantly the numbers of goblet cells in the crypt of the cecum. High-throughput 16S rRNA gene sequencing revealed distinct differences in microbial compositions between the ileum and cecum. XOS500 supplementation significantly increased the bacterial diversity. However, CTC treatment markedly reduced the microbial diversity (P < 0.05). Meanwhile, XOS500 supplementation in the diet significantly increased the abundance of Lactobacillus genus compared to the CON and CTC group in the ileum and cecum (P < 0.01), whereas the level of Clostridium_sensu_stricto_1, Escherichia-Shigella, and Terrisporobacter genus in the XOS500 group were markedly lower than the CON and CTC group (P < 0.05). In addition, dietary supplementation with XOS500 significantly increased the total short-chain fatty acids, propionate and butyrate concentrations and decreased the acetate concentration compared to the CON group in the cecum (P < 0.05). In summary, dietary supplemented with XOS500 could enhance specific beneficial microbiota abundance and decrease harmful microbiota abundance to maintain the structure of the intestinal morphology and improve growth performance of weaned piglets. Thus, XOS may potentially function as an alternative to in-feed antibiotics in weaned piglets in modern husbandry.

scholarly journals Influence of Bacterial Organic Selenium on Blood Parameters, Immune Response, Selenium Retention and Intestinal Morphology of Broiler Chickens

2020 ◽  
Author(s):  
A. M. Dalia ◽  
T. C. Loh ◽  
A. Q. Sazili ◽  
Anjas Asmara Samsudin
Keyword(s):  
Broiler Chickens ◽  
Hematological Parameters ◽  
Basal Diet ◽  
Blood Parameters ◽  
Intestinal Morphology ◽  
Control Group ◽  
Villus Height ◽  
Organic Selenium ◽  
Significant Difference ◽  
Immunity Response

Abstract Background: Several studies indicated that dietary organic selenium (Se) usually absorbed better than an inorganic source, with high retention and bioavailability. Dietary Se as an antioxidant element affects the immune system and hematological status in animals. Therefore, the aim of this study was to evaluate the effect of dietary supplementation of bacterial selenium as an organic source on hematology, immunity response, selenium retention, and gut morphology in broiler chickens. Results: The present results revealed that supplementation of inorganic Se was associated with the lowest level of RBC, HB, and PCV with significant difference than ADS18-Se. In the starter stage, both T2 and T5 were associated with the significantly highest IgG level compared to the basal diet, while all supplemented groups showed higher IgM levels compared to the control group. In the finisher phase, all Se supplemented groups showed significant (P ˂ 0.05) increases in IgG, IgA, and IgM levels compared to T1. Birds fed bacterial-Se showed high intestinal villus height and better Se retention more than sodium selenite. The organic selenium of ADS18 had a superior action in improving Se retention compared to ADS1 and ADS2 bacterial Se. Conclusion: Bacterial organic Se had a beneficial effect on the villus height of small intestine led to high Se absorption and retention. Thus, it caused a better effect of Se on hematological parameters and immunity response.

scholarly journals Intestinal Morphology in Broiler Chickens Supplemented with Propolis and Bee Pollen

Animals ◽  
2019 ◽  
Vol 9 (6) ◽  
pp. 301 ◽  
Author(s):  
Ivana Prakatur ◽  
Maja Miskulin ◽  
Mirela Pavic ◽  
Ksenija Marjanovic ◽  
Valerija Blazicevic ◽  
...  
Keyword(s):  
Beneficial Effect ◽  
Broiler Chickens ◽  
Dietary Supplementation ◽  
Basal Diet ◽  
Intestinal Morphology ◽  
Control Group ◽  
Villus Height ◽  
Bee Pollen ◽  
Crypt Depth ◽  
Surface Areas

The aim of this study was to determine the influence of dietary supplementation with propolis and bee pollen on the intestinal morphology and absorptive surface areas of chickens. Two hundred day-old Ross 308 chickens (100 male and 100 female) were equally allocated into five groups. Throughout the whole study, the control group of chickens was fed with a basal diet, while the experimental groups of chickens were fed with the same diet supplemented with propolis and bee pollen: P1 = 0.25 g of propolis/kg + 20 g of bee pollen/kg; P2 = 0.5 g of propolis/kg; P3 = 1.0 g of propolis/kg; P4 = 20 g of bee pollen/kg. The duodenal villi of chickens from all experimental groups were significantly higher and wider (p < 0.001), while their duodenal villi crypts were significantly deeper (p < 0.001) in comparison with these parameters in chickens from the control group. The villus height to crypt depth ratio, as well as the absorptive surface areas of broiler chickens, were significantly increased (p < 0.001) in experimental groups of chickens in comparison with the control group. These findings suggest that dietary supplementation with propolis and bee pollen has a beneficial effect on broilers chickens’ intestinal morphophysiology.

scholarly journals Influence of Bacterial Organic Selenium on Blood Parameters, Immune Response, Selenium Retention and Intestinal Morphology of Broiler Chickens

2020 ◽  
Author(s):  
A. M. Dalia ◽  
T. C. Loh ◽  
A. Q. Sazili ◽  
Anjas Asmara Samsudin
Keyword(s):  
Broiler Chickens ◽  
Hematological Parameters ◽  
Basal Diet ◽  
Blood Parameters ◽  
Intestinal Morphology ◽  
Control Group ◽  
Villus Height ◽  
Organic Selenium ◽  
Significant Difference ◽  
Immunity Response

Abstract Background: Several studies indicated that dietary organic selenium (Se) usually absorbed better than an inorganic source, with high retention and bioavailability. Dietary Se as an antioxidant element affects the immune system and hematological status in animals. Therefore, the aim of this study was to evaluate the effect of dietary supplementation of bacterial selenium as an organic source on hematology, immunity response, selenium retention, and gut morphology in broiler chickens. Results: The present results revealed that supplementation of inorganic Se was associated with the lowest level of RBC, HB, and PCV with significant difference than ADS18-Se. In the starter stage, both T2 and T5 were associated with the significantly highest IgG level compared to the basal diet, while all supplemented groups showed higher IgM levels compared to the control group. In the finisher phase, all Se supplemented groups showed significant (P ˂ 0.05) increases in IgG, IgA, and IgM levels compared to T1. Birds fed bacterial-Se showed high intestinal villus height and better Se retention more than sodium selenite. The organic selenium of ADS18 had a superior action in improving Se retention compared to ADS1 and ADS2 bacterial Se. Conclusion: Bacterial organic Se had a beneficial effect on the villus height of small intestine led to high Se absorption and retention. Thus, it caused a better effect of Se on hematological parameters and immunity response.

scholarly journals Effects of Plotytarya strohilacea Sieb. et Zuce Tannin on the Growth Performance, Oxidation Resistance, Intestinal Morphology and Cecal Microbial Composition of Broilers

2022 ◽  
Vol 8 ◽  
Author(s):  
Zhenkai Tong ◽  
Fuhong Lei ◽  
Lixuan Liu ◽  
Fei Wang ◽  
Aiwei Guo
Keyword(s):  
Growth Performance ◽  
Basal Diet ◽  
Intestinal Morphology ◽  
Control Group ◽  
Villus Height ◽  
Microbial Composition ◽  
Antioxidant Function ◽  
Total Antioxidant ◽  
Quadratic Relationship ◽  
Addition Amount

The purpose of this experiment was to study the effects of Plotytarya strohilacea Sieb. et Zuce tannin on broilers growth performance, antioxidant function, intestinal development, intestinal morphology and the cecal microbial composition. In this experiment, a total of 360 1-day-old Arbor Acres male broilers were randomly divided into 4 treatment groups, with 6 replicates in each group and 15 broilers in each replicate. The control group (Control) was fed the basal diet, and the broilers were fed a basal diet supplemented with 0 (Control), 100 (PT1), 400 (PT2), and 800 (PT3) mg/kg Plotytarya strohilacea Sieb. et Zuce tannins for 42 days, respectively. The results showed that the average daily feed intake (ADFI) of the PT1 group was significantly lower than that of the control group, and there was a significant quadratic relationship between the ADFI and the concentration of tannin (P &lt; 0.05). Compared with the control group, the F/G of broilers during the 22–42 days phase in the PT1 group showed a decreasing trend (P = 0.063). The serum catalase (CAT) activity in the PT1 group was significantly higher than those of the other three groups, and the effect was significantly quadratically related to the dosage (P &lt; 0.05). The glutathione peroxidase (GSH-Px) activity in the PT1, PT2 and control groups were significantly higher than that of the PT3 group, and the effect was significantly quadratically related to the addition amount (P &lt; 0.05). The serum total antioxidant capacity (T-AOC) activity in the PT1 group was significantly higher than that in the control group, and the effect was significantly quadratically related to the addition amount (P &lt; 0.05). Compared to the control group, the villus height of jejunum in the PT1, PT2 and PT3 groups were significantly higher, and there was a significant quadratic relationship between the villus height of jejunum and the addition amount (P &lt; 0.05). In addition, adding tannins to diets significantly increased Parabacteroides in the dominant genus (P &lt; 0.05). In conclusion, dietary supplementation with Plotytarya strohilacea Sieb. et Zuce tannin improved the growth performance, antioxidant function, and intestinal morphology along with an increased abundance of Parabacteroides in the cecum, and the recommended dosage of tannin in broiler diets was 100 mg/kg.

scholarly journals Influence of bacterial organic selenium on blood parameters, immune response, selenium retention and intestinal morphology of broiler chickens

2020 ◽  
Vol 16 (1) ◽  
Author(s):  
A. M. Dalia ◽  
T. C. Loh ◽  
A. Q. Sazili ◽  
A. A. Samsudin
Keyword(s):  
Broiler Chickens ◽  
Hematological Parameters ◽  
Basal Diet ◽  
Blood Parameters ◽  
Intestinal Morphology ◽  
Control Group ◽  
Villus Height ◽  
Organic Selenium ◽  
Significant Difference ◽  
Immunity Response

Abstract Background Several studies indicated that dietary organic selenium (Se) usually absorbed better than an inorganic source, with high retention and bioavailability. Dietary Se as an antioxidant element affects the immune system and hematological status in animals. Therefore, the aim of this study was to evaluate the effect of dietary supplementation of bacterial selenium as an organic source on hematology, immunity response, selenium retention, and gut morphology in broiler chickens. Results The present results revealed that supplementation of inorganic Se was associated with the lowest level of RBC, HB, and PCV with significant difference than ADS18-Se. In the starter stage, both T2 and T5 were associated with the significantly highest IgG level compared to the basal diet, while all supplemented groups showed higher IgM levels compared to the control group. In the finisher phase, all Se supplemented groups showed significant (P ˂ 0.05) increases in IgG, IgA, and IgM levels compared to T1. Birds fed bacterial-Se showed high intestinal villus height and better Se retention more than sodium selenite. The organic selenium of ADS18 had a superior action in improving Se retention compared to ADS1 and ADS2 bacterial Se. Conclusions Bacterial organic Se had a beneficial effect on the villus height of small intestine led to high Se absorption and retention. Thus, it caused a better effect of Se on hematological parameters and immunity response.

scholarly journals Algae-Derived Polysaccharides Promote Growth Performance by Improving Antioxidant Capacity and Intestinal Barrier Function in Broiler Chickens

2020 ◽  
Vol 7 ◽  
Author(s):  
Wen-Chao Liu ◽  
Yan Guo ◽  
Zhi-Hui Zhao ◽  
Rajesh Jha ◽  
Balamuralikrishnan Balasubramanian
Keyword(s):  
Antioxidant Capacity ◽  
Growth Performance ◽  
Average Daily Gain ◽  
Intestinal Morphology ◽  
Intestinal Barrier Function ◽  
Control Group ◽  
Gut Health ◽  
Feed Conversion ◽  
Villus Height ◽  
Final Body Weight

This study aimed to determine the efficacy of dietary algae-derived polysaccharides (ADPs) from Enteromorpha on growth performance, intestinal morphology, intestinal permeability, and antioxidant capacity in serum, liver, and intestinal mucosa of broilers. Three hundred and ninety six day-old male chicks were randomly assigned to six dietary treatments containing 0 (Control), 1,000, 2,500, 4,000, 5,500, and 7,000 mg ADP/kg basal diet in a 35 day feeding trial. During day 1–21, compared with the control group, dietary 1,000–7,000 mg/kg ADP supplementation improved the average daily gain (ADG) and feed conversion ratio (p &lt; 0.05). Overall (day 1–35), dietary inclusion of 1,000 mg/kg ADP increased the final body weight and ADG (p &lt; 0.05). Besides, on day 21, dietary 2,500 mg/kg ADP supplementation increased the serum catalase (CAT) and liver total superoxide dismutase (T-SOD) activities (p &lt; 0.05), whereas dietary 1,000–5,500 mg/kg ADP supplementation decreased malondialdehyde (MDA) contents in serum and liver (p &lt; 0.05). On day 35, supplementation of 1,000 mg/kg ADP increased the serum glutathione peroxidase and CAT activities and liver T-SOD activities (p &lt; 0.05). It decreased the MDA level of serum and liver (p &lt; 0.05). Also, dietary 2,500 mg/kg ADP increased the villus height of jejunum and ileum on day 21 (p &lt; 0.05), and dietary 4,000 mg/kg ADP increased the villus height of duodenum and ileum on day 35 (p &lt; 0.05). On day 21, dietary 4,000 mg/kg ADP increased the CAT activities of the duodenum and T-SOD activities of jejunum and ileum and decreased the MDA contents in the duodenum, jejunum, and ileum (p &lt; 0.05). On day 35, dietary inclusion of 1,000–7,000 mg/kg ADP reduced MDA contents of duodenum and jejunum (p &lt; 0.05). Furthermore, dietary inclusion of ADP at 1,000–7,000 mg/kg decreased serum DAO activities at day 21 and day 35 (p &lt; 0.05), and the serum D-lactic acid concentration was reduced by dietary supplementation of 1,000, 2,500, and 7,000 mg/kg ADP on day 21. In conclusion, dietary ADP exerted beneficial effects on growth performance, antioxidant capacity, and gut health in broilers; based on the studied parameters, the appropriate recommended dose is 1,000–4,000 mg/kg. These findings provided new insights into the potential application of ADP as natural growth promoters in broilers.

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