scholarly journals Coronatine contributes to Pseudomonas cannabina pv. alisalensis virulence by overcoming both stomatal and apoplastic defenses in dicot and monocot plants

2021 ◽  
Author(s):  
Nanami Sakata ◽  
Takako Ishiga ◽  
Shunsuke Masuo ◽  
Yoshiteru Hashimoto ◽  
Yasuhiro Ishiga
Keyword(s):  
Virulence Factors ◽  
Causative Agent ◽  
Green Manure ◽  
Bacterial Blight ◽  
Host Plants ◽  
Deletion Mutants ◽  
Tomato Dc3000 ◽  
Wide Range ◽  
Infection Processes ◽  
Green Manure Crops

Pseudomonas cannabina pv. alisalensis (Pcal) is a causative agent of bacterial blight of crucifer including cabbage, radish, and broccoli. Importantly, Pcal can infect not only a wide range of Brassicaceae, but also green manure crops such as oat. However, Pcal virulence mechanisms have not been investigated and are not fully understood. We focused on coronatine (COR) function, which is one of the well-known P. syringae pv. tomato DC3000 virulence factors, in Pcal infection processes on both dicot and monocot plants. Cabbage and oat plants dip-inoculated with a Pcal KB211 COR mutant (ΔcmaA) exhibited reduced virulence compared to Pcal WT. Moreover, ΔcmaA failed to reopen stomata on both cabbage and oat, suggesting that COR facilitates Pcal entry through stomata into both plants. Furthermore, cabbage and oat plants syringe-infiltrated with ΔcmaA also showed reduced virulence, suggesting that COR is involved in overcoming not only stomatal-based defense, but also apoplastic defense. Indeed, defense related genes, including PR1 and PR2, were highly expressed in plants inoculated with ΔcmaA compared to Pcal WT, indicating that COR suppresses defense-related genes of both cabbage and oat. Additionally, SA accumulation increases after ΔcmaA inoculation compared to Pcal WT. Taken together, COR contributes to cause disease by suppressing stomatal-based defense and apoplastic defense in both dicot and monocot plants. Here, we investigated COR functions in the interaction of Pcal and different host plants (dicot and monocot plants) using genetically and biochemically defined COR deletion mutants.

scholarly journals Coronatine contributes Pseudomonas cannabina pv. alisalensis virulence by overcoming both stomatal and apoplastic defenses in dicot and monocot plants

2020 ◽  
Author(s):  
Nanami Sakata ◽  
Takako Ishiga ◽  
Shunsuke Masuo ◽  
Yoshiteru Hashimoto ◽  
Yasuhiro Ishiga
Keyword(s):  
Pseudomonas Syringae ◽  
Virulence Factors ◽  
Bacterial Blight ◽  
Host Plants ◽  
Disease Outbreaks ◽  
Brown Spot ◽  
Wide Range ◽  
Infection Processes ◽  
Cruciferous Plants ◽  
Green Manure Crops

AbstractP. cannabina pv. alisalensis (Pcal) is a causative agent of bacterial blight of crucifer including cabbage, radish, and broccoli. Importantly, Pcal can infect not only a wide range of Brassicaceae, but also green manure crops such as oat. However, Pcal virulence mechanisms have not been investigated and are not fully understood. We focused on coronatine (COR) function, which is one of the well-known P. syringae pv. tomato DC3000 virulence factors, in Pcal infection processes on both dicot and monocot plants. Cabbage and oat plants dip-inoculated with a Pcal KB211 COR mutant (ΔcmaA) exhibited reduced virulence compared to Pcal WT. Moreover, ΔcmaA failed to reopen stomata on both cabbage and oat, suggesting that COR facilitates Pcal entry through stomata into both plants. Furthermore, cabbage and oat plants syringe-infiltrated with ΔcmaA also showed reduced virulence, suggesting that COR is involved in overcoming not only stomatal-based defense, but also apoplastic defense. Indeed, defense related genes, including PR1 and PR2, were highly expressed in plants inoculated with ΔcmaA compared to Pcal WT, indicating that COR suppresses defense-related genes of both cabbage and oat. Additionally, SA accumulation increases after ΔcmaA inoculation compared to Pcal WT. Taken together, COR contributes to cause disease by suppressing stomatal-based defense and apoplastic defense in both dicot and monocot plants. This is the first study to investigate COR functions in the interaction of Pcal and different host plants (dicot and monocot plants) using genetically and biochemically defined COR deletion mutants.Author summaryDisease outbreaks caused by new Pseudomonas syringae isolates are problems worldwide. P. cannabina pv. alisalensis (Pcal) causes bacterial blight on a wide range of cruciferous plants and bacterial brown spot on oat plants. Although P. syringae deploys a variety of virulence factors, Pcal virulence factors have not been investigated. We focused on coronatine (COR) function, which is one of the well-known P. syringae virulence factors. COR is a non-host-specific phytotoxin and contributes to P. syringae growth and lesion formation or expansion in several host plants. COR function has been mainly studied in the model pathogen P. syringae pv. tomato DC3000 and model the plant Arabidopsis thaliana. Thus, COR roles in Pcal infection especially on monocot plants have not been well studied. Therefore, we investigated COR role in Pcal interaction with both dicot and monocot plants. Here, we revealed that COR functions as a multifunctional suppressor to manage Pcal virulence on both plants.

scholarly journals PERSPECTIVES OF BREEDING OF VEGETABLE CROPS OF FABACEAE FAMILY TO BEAN YELLOW MOSAIC VIRUS (Potyvirus, Potyviridae) RESISTANCE IN THE CONDITIONS OF MOSCOW REGION

2018 ◽  
pp. 77-83
Author(s):  
I. A. Engalycheva ◽  
E. G. Kozar ◽  
A. A. Antoshkin ◽  
E. P. Pronina ◽  
Y. G. Volkov ◽  
...  
Keyword(s):  
Causative Agent ◽  
Host Plants ◽  
Far East ◽  
Diagnostic Methods ◽  
Moscow Region ◽  
Kidney Bean ◽  
Yellow Mosaic Virus ◽  
Distribution Area ◽  
Sources Of Resistance ◽  
Wide Range

In the context of climate change phytomonitoring of the prevalence of the most common viral pathogens on the crops becomes even more important, because during the last decades the harmfulness of those pathogens, in particular Bean yellow mosaic (BYMV) has grown in intensity. The causative agent as the most members of Рotyvirus genus, has a wide range of host plants belonging to various families including Fabaceae. In Russia the virus was for the first time identified in the south of the Far East, where in the middle of XX century the massive damage of clove, lupine, sweet pea, pea, bean and Russian bean was observed. The distribution area of BYMV considerably expanded after advancement of heat-loving leguminous crops towards north. During the last years epiphytotics were reported in the planted crops of kidney bean (Phasйolus vulgбris L.), and Russian bean (Faba bona Medik. var. major Harz.) under conditions of temperate continental climate of nonchernozem belt in the RF. It is not feasible to eradicate natural BYMV foci, while the control of the causative agent carriers as a preventive measure is not very effective. There fore at present the search for the sources of resistance to BYMV and creation of parent selection material is a priority area of our phytopatologic research. At the present stage the tasks included: identification and study of the properties of BYMV Moscow isolates; integral assessment of resistance and other economically valuable characteristics of collection material and breeding stock material of kidney bean (810 specimens) and Russian bean (40 specimens) generated in the Laboratory of Legume Selection and Seed Production, Federal State Budgetary Scientific Institution “Federal Scientific Vegetable Center”. To achieve the goal visual, serological diagnostic methods were used together with biotesting and electron microscopy. The research revealed special physical-chemical characteristics of BYMV Moscow isolates characterized by 100% harmfulness when occurred as co-infection with the other viruses. The main symptoms caused by the above isolates in indicator plants and host plants under conditions of Moscow Region have been described. Evaluation of collection and selection materials of kidney bean and Russian bean formed the basis for identification of the sources of resistance to BYMV exhibiting integrated economically valuable properties (early ripeness, bean shape and color, productivity, etc.). These specimens have been included into selection program of the Federal Research Vegetable Center which is aimed on creation of high-productive varieties of the kidney bean meeting the modern market demands.

Xylophilus ampelinus (canker of grapevine).

2021 ◽  
Author(s):  
Tanja Dreo
Keyword(s):  
Vitis Vinifera ◽  
Causative Agent ◽  
Bacterial Blight ◽  
Host Plants ◽  
Surrounding Area ◽  
Planting Material ◽  
Xylophilus Ampelinus ◽  
Slow Growing ◽  
Natural Dispersal

Abstract The causative agent of bacterial blight of grapevine, X. ampelinus, is a slow-growing bacterium. It is thought that only European grapevines (Vitis vinifera) are susceptible (Panagopoulos, 1988b). Natural dispersal from infected plants is limited to the vineyard and the immediately surrounding area and is mostly associated with pruning. Dispersal over large distances is expected to occur only associated with host plants, most likely during trading of planting material. It is difficult to control as outbreaks are sporadic and infections are often latent thus hindering detection.

scholarly journals Transposon mutagenesis reveals Pseudomonas cannabina pv. alisalensis optimizes its virulence factors for pathogenicity on different hosts

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7698 ◽  
Author(s):  
Nanami Sakata ◽  
Takako Ishiga ◽  
Haruka Saito ◽  
Viet Tru Nguyen ◽  
Yasuhiro Ishiga
Keyword(s):  
Virulence Factors ◽  
Type Iii Secretion ◽  
Bacterial Blight ◽  
Membrane Transporters ◽  
Plant Host ◽  
Inoculation Test ◽  
Bacterial Interaction ◽  
Dicotyledonous Plants ◽  
Blight Disease ◽  
Green Manure Crops

Pseudomonas cannabina pv. alisalensis (Pcal), which causes bacterial blight disease of Brassicaceae, is an economically important pathogen worldwide. To identify Pcal genes involved in pathogenesis, we conducted a screen for 1,040 individual Pcal KB211 Tn5 mutants with reduced virulence on cabbage plants using a dip-inoculation method. We isolated 53 reduced virulence mutants and identified several potential virulence factors involved in Pcal virulence mechanisms such as the type III secretion system, membrane transporters, transcription factors, and amino acid metabolism. Importantly, Pcal is pathogenic on a range of monocotyledonous and dicotyledonous plants. Therefore, we also carried out the inoculation test on oat plants, which are cultivated after cabbage cultivation as green manure crops. Interestingly among the 53 mutants, 31 mutants also exhibited reduced virulence on oat seedlings, indicating that Pcal optimizes its virulence factors for pathogenicity on different host plants. Our results highlight the importance of revealing the virulence factors for each plant host-bacterial interaction, and will provide new insights into Pcal virulence mechanisms.

scholarly journals Selected Legumes Used As Summer Cover Crops

EDIS ◽  
1969 ◽  
Vol 2003 (16) ◽  
Author(s):  
Jim Rich ◽  
David Wright ◽  
Jim Marois ◽  
Dick Sprenkel
Keyword(s):  
Cover Crops ◽  
Cover Crop ◽  
Green Manure ◽  
Cooperative Extension Service ◽  
Extension Service ◽  
Publication Date ◽  
University Of Florida ◽  
Wide Range ◽  
Pest Suppression ◽  
Green Manure Crops

Cover crops are generally planted after a primary (cash) crop for one or more of the following reasons: erosion control, organic matter accumulation, improved soil tilth, pest suppression (weed, disease, nematode, and insect), and nitrogen production. Optimally, a cover crop will provide a wide range of most or all of the previously mentioned benefits, even if the main reason for planting it was more specific. The eight cover/green manure crops described herein have been shown to provide several benefits to a succeeding crop. This document is ENY-688 (IN483), one of a series of the Department of Entomology and Nematology, Florida Cooperative Extension Service, Institute of Food and Agricultural Sciences, University of Florida, Publication Date: August 2003.

scholarly journals Characterization and Genetic Diversity of Listeria monocytogenes Isolated from Cattle Abortions in Latvia, 2013–2018

2021 ◽  
Vol 8 (9) ◽  
pp. 195
Author(s):  
Žanete Šteingolde ◽  
Irēna Meistere ◽  
Jeļena Avsejenko ◽  
Juris Ķibilds ◽  
Ieva Bergšpica ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Listeria Monocytogenes ◽  
Virulence Factors ◽  
Causative Agent ◽  
Animal Species ◽  
Clonal Complex ◽  
The Third ◽  
Wide Range ◽  
Sequence Types

Listeria monocytogenes can cause disease in humans and in a wide range of animal species, especially in farm ruminants. The aim of the study was to determine the prevalence and genetic diversity of L. monocytogenes related to 1185 cattle abortion cases in Latvia during 2013–2018. The prevalence of L. monocytogenes among cattle abortions was 16.1% (191/1185). The seasonality of L. monocytogenes abortions was observed with significantly higher occurrence (p < 0.01) in spring (March–May). In 61.0% of the cases, the affected cattle were under four years of age. L. monocytogenes abortions were observed during the third (64.6%) and second (33.3%) trimesters of gestation. Overall, 27 different sequence types (ST) were detected, and four of them, ST29 (clonal complex, CC29), ST37 (CC37), ST451 (CC11) and ST7 (CC7), covered more than half of the L. monocytogenes isolates. Key virulence factors like the prfA-dependent virulence cluster and inlA, inlB were observed in all the analyzed isolates, but lntA, inlF, inlJ, vip were associated with individual sequence types. Our results confirmed that L. monocytogenes is the most important causative agent of cattle abortions in Latvia and more than 20 different STs were observed in L. monocytogenes abortions in cattle.

scholarly journals Evaluation of Peanut and Four Green Manure Crops as Host Plants for Scepticus uniformis（Coleoptera: Curculionidae）in a Frame Field

2019 ◽  
Vol 63 (4) ◽  
pp. 189-197 ◽  
Author(s):  
Hiroshi Oida ◽  
Hiroshi Fukuda ◽  
Kosuke Hotchi ◽  
Toshio Shimizu ◽  
Kenji Suzuki
Keyword(s):  
Green Manure ◽  
Host Plants ◽  
Frame Field ◽  
Green Manure Crops

scholarly journals Virulence factors of Actinobacillus actinomycetemcomitans: other putative factors

2000 ◽  
Vol 14 (1) ◽  
pp. 05-11
Author(s):  
Mario Julio AVILA-CAMPOS ◽  
Maria Regina Lorenzetti SIMIONATO ◽  
Silvana CAI ◽  
Márcia Pinto Alves MAYER ◽  
José Luiz DE LORENZO ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Periodontal Disease ◽  
Virulence Factors ◽  
Causative Agent ◽  
Tissue Repair ◽  
Bacteriocin Production ◽  
Actinobacillus Actinomycetemcomitans ◽  
Blood Types ◽  
Wide Range ◽  
Localized Juvenile Periodontitis

Actinobacillus actinomycetemcomitans is implicated as the causative agent of localized juvenile periodontitis. This organism possesses a large number of virulence factors with a wide range of activities and also interfere with tissue repair. Fifty isolates of A. actinomycetemcomitans from 20 periodontal patients were examined to evaluate other putative virulence factors. In this study, the capsule, DNase, coagulase, fibrinolysin, proteolytic, haemolysin and bacteriocin production, haemagglutination, serum sensitivity, epithelial cells attachment, hydrophobicity and virulence of the A. actinomycetemcomitans isolates were evaluated. All the isolates were resistant to the different tested sera. 70% to 94% were alpha-haemolytics and agglutinated all blood types. Most of isolates produced antagonistic substances and they had a low hydrophobicity. None of the isolates was pathogenic for mice. Little is known as to wether these factors may act in the development of periodontal disease, and further studies are required for an application in pathogenic and systematic terms.

The efficiency of the crop rotations with short rotation with different levels of their saturation of cereals and crops in the conditions of Western Forest-Steppe

2017 ◽  
Vol 1 (92) ◽  
pp. 62-68
Author(s):  
R. Holod ◽  
О. Bilinska ◽  
H. Shubala
Keyword(s):  
Crop Rotation ◽  
Green Manure ◽  
Water Regime ◽  
Crop Rotations ◽  
Forest Steppe ◽  
Field Crop ◽  
Soil Water Regime ◽  
Short Rotation ◽  
Arable Farming ◽  
Green Manure Crops

There were analyzed and disclosed the basic components of arable farming systems and their Meaning, the current state and scientific principles in the context of the further development of field crop cultivation in the conditions of Western Forest-Steppe. The purpose of research. To study an effect of alternation of crop in crop rotation in conditions of brief rotation on the soil water regime, productivity and economic efficiency. Methods. Field, laboratory, comparative and analytical. Results. The results of researches on study of productivity of four-field crop rotations with short rotation depending on their saturation by the grain and tilled cultures, of various use of mineral fertilizers, green manure crops and collateral products which were conducted during 2014-2015 in the stationary experiment of the scientific and technological department of plant growing and arable farming, of the TDSGDS of the IKSGP of NAAN are resulted In the article. The elements of the biologization of farming are the basis of our development of crop rotations with short rotation. The study of the effect of green manure crops and collateral products in four-field crop rotations with a different set of crops on the change of soil fertility and productivity of crop rotations as a whole was carried out to this purpose. According to the results of the research, is provided the information on the effectiveness of improving the field crop rotations with short rotation with varying degrees of saturation by grain and tilled crops, that ensure the production of environmentally friendly products, reducing the cost of grain, improving the quality of marketable products. The study of the effect of alternation of crop in crop rotation in conditions of brief rotation on the soil water regime, productivity and economic efficiency showed that an increase in crop rotation productivity is observed in short-rotation crop rotations, if they are saturated by grain crops up to 100%, cereals crops reduction to 50% in crop rotations contributes to a decrease in crop productivity. Conclusion. Thus, the results of the research showed that with the correct construction of short rotational crop rotations, such problems as rational use of nutrients and soil moisture, control of weeds and pests of agricultural crops, improvement of the physical and chemical properties of the soil, increased efficiency in the use of fertilizers and equipment, Cheapening of the received agricultural product may be solved.

scholarly journals Pseudomonas aeruginosa PA80 is a cystic fibrosis isolate deficient in RhlRI quorum sensing

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Syed A. K. Shifat Ahmed ◽  
Michelle Rudden ◽  
Sabrina M. Elias ◽  
Thomas J. Smyth ◽  
Roger Marchant ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Quorum Sensing ◽  
Virulence Factors ◽  
Genomic Islands ◽  
Clinical Strain ◽  
Whole Genome ◽  
Synonymous Mutations ◽  
Wide Range

AbstractPseudomonas aeruginosa uses quorum sensing (QS) to modulate the expression of several virulence factors that enable it to establish severe infections. The QS system in P. aeruginosa is complex, intricate and is dominated by two main N-acyl-homoserine lactone circuits, LasRI and RhlRI. These two QS systems work in a hierarchical fashion with LasRI at the top, directly regulating RhlRI. Together these QS circuits regulate several virulence associated genes, metabolites, and enzymes in P. aeruginosa. Paradoxically, LasR mutants are frequently isolated from chronic P. aeruginosa infections, typically among cystic fibrosis (CF) patients. This suggests P. aeruginosa can undergo significant evolutionary pathoadaptation to persist in long term chronic infections. In contrast, mutations in the RhlRI system are less common. Here, we have isolated a clinical strain of P. aeruginosa from a CF patient that has deleted the transcriptional regulator RhlR entirely. Whole genome sequencing shows the rhlR locus is deleted in PA80 alongside a few non-synonymous mutations in virulence factors including protease lasA and rhamnolipid rhlA, rhlB, rhlC. Importantly we did not observe any mutations in the LasRI QS system. PA80 does not appear to have an accumulation of mutations typically associated with several hallmark pathoadaptive genes (i.e., mexT, mucA, algR, rpoN, exsS, ampR). Whole genome comparisons show that P. aeruginosa strain PA80 is closely related to the hypervirulent Liverpool epidemic strain (LES) LESB58. PA80 also contains several genomic islands (GI’s) encoding virulence and/or resistance determinants homologous to LESB58. To further understand the effect of these mutations in PA80 QS regulatory and virulence associated genes, we compared transcriptional expression of genes and phenotypic effects with isogenic mutants in the genetic reference strain PAO1. In PAO1, we show that deletion of rhlR has a much more significant impact on the expression of a wide range of virulence associated factors rather than deletion of lasR. In PA80, no QS regulatory genes were expressed, which we attribute to the inactivation of the RhlRI QS system by deletion of rhlR and mutation of rhlI. This study demonstrates that inactivation of the LasRI system does not impact RhlRI regulated virulence factors. PA80 has bypassed the common pathoadaptive mutations observed in LasR by targeting the RhlRI system. This suggests that RhlRI is a significant target for the long-term persistence of P. aeruginosa in chronic CF patients. This raises important questions in targeting QS systems for therapeutic interventions.

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