scholarly journals Effects of Temperature and Wetness Duration on the Sporulation Rate of Phomopsis viticola on Infected Grape Canes

Plant Disease ◽  
2013 ◽  
Vol 97 (5) ◽  
pp. 579-589 ◽  
Author(s):  
D. J. Anco ◽  
L. V. Madden ◽  
M. A. Ellis

Controlled-environment studies were conducted to examine effects of temperature (T) and wetness duration (W) on the sporulation rate of Phomopsis viticola on infected grape canes and to determine effects of interrupted wetness duration (IWD) on sporulation. A split-plot design was used to determine T and W effects, with T (5, 12, 15, 18, 20, 22, 25, 28, and 35°C) as the whole-plot and W (11, 23, 35, 47, and 71 h) as the subplot. Linear and nonlinear mixed models were fitted to the data. Lower and upper limits of sporulation were estimated to be 4 and 36°C, respectively, based on the modeling results, optimum sporulation was near 21°C, and sporulation increased monotonically with increasing wetness duration. Of the examined models, a generalization of the Analytis Beta model fit the data best, based on a collection of goodness-of-fit statistical criteria. To determine effects of IWD, a split-plot was used, with T (12, 15, and 20°C) as the whole-plot and IWD (0, 2, 4, 8, 12, and 24 h) as the subplot. Generally, sporulation declined with increasing IWD. An IWD of 8 h or more resulted in significantly and substantially less sporulation compared to the control (0 h IWD) (P < 0.01). Temporal patterns of spore density in the field were determined using a repeated-measures design, in which spore density and environmental data were measured in the vineyard during and following individual rain events over 3 years. The developed model from the controlled-environment study, coupled with a time-of-season weight function and a dispersal index (based on total rain per rain episode), predicted the trend in spore density over time reasonably well, although the total magnitude of spore density could not be predicted because the density of lesions was not known. Results can be used for improving the accuracy of a disease warning system that currently only considers infection of grapes by P. viticola.

2012 ◽  
Vol 13 (1) ◽  
pp. 27
Author(s):  
Daniel J. Anco ◽  
Laurence V. Madden ◽  
Michael A. Ellis

In 2008, research was initiated to examine effects of temperature and wetness duration on the sporulation of Phomopsis viticola on infected grape canes and to determine effects of interrupted wetness duration on sporulation. To determine effects of temperature (T) and wetness duration (WD) on sporulation, a split-plot experimental design was used, with T (5, 12, 15, 18, 20, 22, 25, 28, and 35°C) assigned to whole-plots and WD (11, 23, 35, 47, and 71 h) assigned to sub-plots. Linear and nonlinear mixed models were fitted to the data. Lower and upper limits of sporulation were found to be at 5 and 35°C, respectively. Optimum sporulation was near 22°C, and sporulation increased with increasing WD. Of the examined models, a generalized Analytis Beta model fit the data best. To determine effects of wetness interruption (IWD), a split-plot was used, with T (12, 15, and 20°C) assigned to whole-plots and IWD (0, 2, 4, 8, 12, and 24 h) assigned to sub-plots. Generally, sporulation declined with increasing IWD. An IWD of 12 h or more resulted in significantly and substantially less sporulation compared to the control (0 h IWD). Using a repeated-measures design, spore density and environmental data were measured in the vineyard during and following individual rain events; a preliminary model predicted the temporal trend in spore density within the vineyard fairly well (R2 = 0.719), although absolute magnitude of sporulation could not be predicted. Accepted for publication 27 April 2012. Published 23 July 2012.


Methodology ◽  
2017 ◽  
Vol 13 (1) ◽  
pp. 9-22 ◽  
Author(s):  
Pablo Livacic-Rojas ◽  
Guillermo Vallejo ◽  
Paula Fernández ◽  
Ellián Tuero-Herrero

Abstract. Low precision of the inferences of data analyzed with univariate or multivariate models of the Analysis of Variance (ANOVA) in repeated-measures design is associated to the absence of normality distribution of data, nonspherical covariance structures and free variation of the variance and covariance, the lack of knowledge of the error structure underlying the data, and the wrong choice of covariance structure from different selectors. In this study, levels of statistical power presented the Modified Brown Forsythe (MBF) and two procedures with the Mixed-Model Approaches (the Akaike’s Criterion, the Correctly Identified Model [CIM]) are compared. The data were analyzed using Monte Carlo simulation method with the statistical package SAS 9.2, a split-plot design, and considering six manipulated variables. The results show that the procedures exhibit high statistical power levels for within and interactional effects, and moderate and low levels for the between-groups effects under the different conditions analyzed. For the latter, only the Modified Brown Forsythe shows high level of power mainly for groups with 30 cases and Unstructured (UN) and Autoregressive Heterogeneity (ARH) matrices. For this reason, we recommend using this procedure since it exhibits higher levels of power for all effects and does not require a matrix type that underlies the structure of the data. Future research needs to be done in order to compare the power with corrected selectors using single-level and multilevel designs for fixed and random effects.


2000 ◽  
Vol 78 (5) ◽  
pp. 706-712 ◽  
Author(s):  
Shannon D Martin ◽  
David A Gray ◽  
William H Cade

The effects of temperature on the structure of cricket calling song were examined in the trilling field cricket Gryllus integer. A repeated-measures design was used to assess the effects of temperature and individual differences among males simultaneously. Temperature affected most aspects of calling song, specifically pulse length, interpulse length, peak frequency, trill length, intertrill length, and pulse duty cycle. Unaffected by temperature were the number of pulses per trill, the proportion of pulses missed within a trill, and the trill duty cycle. After controlling for temperature, significant individual differences among crickets were found in peak frequency, number of pulses per trill, trill length, and intertrill interval. Both the environmental and individual, that is, possibly genetic, influences on calling song may influence sexual communication in this species.


2019 ◽  
Vol 3 (2) ◽  
Author(s):  
F. A. Ribeiro ◽  
S. K. Lau ◽  
N. Herrera ◽  
M. Henriott ◽  
N. Bland ◽  
...  

ObjectivesThere has been an increased interest in merchandising dry-aged steaks at the retail level. Further understanding of the influence of the dry aging process on meat color and lipid stability is needed to ensure dry-aged beef products can be merchandised without adverse impacts on retail display life. Therefore, this study aimed to determine color and lipid stability of steaks from dry-aged beef loins over 7 d (d) of retail display.Materials and MethodsSixteen USDA low Choice boneless strip loins were assigned to one of four aging treatments: vacuum (Wet), dry-aging at 50% relative humidity (RH) (RH50), dry-aging at 70% RH (RH70), or dry-aging at 85% RH (RH85). Dry-aged loins were placed in each assigned dry aging chamber, while wet aged counterparts were aged in vacuum bags in the same cooler. Loins were aged for 42 d at 1°C. After aging, loins were trimmed of dehydrated lean/fat and fabricated into steaks. Steaks were trimmed of subcutaneous fat, and placed on foam trays, overwrapped with oxygen permeable film and placed under retail display (RD) conditions for 7 d at 2°C. Objective color measurements were taken once daily from d 0 to 7 of RD. Trained visual color panelists (n = 6) evaluated surface discoloration from d 0 to 7 of RD once daily. Lipid oxidation was measured via thiobarbituric acid reactive substance assay (TBARS) at 0, 4, and 7 d of RD. Color data were analyzed as a split-plot repeated measures design with treatment as the whole-plot and RD time as the repeated measures. TBARS data were analyzed as a split-plot design with aging treatment as the whole-plot, and RD time as the split-plot. In this study, chamber (loin) was considered the experimental unit. Data were analyzed using the PROC GLIMMIX procedure of SAS with α = 0.05.ResultsFor all three-color scales, a RD effect was found (P < 0.001). In general, L*, a* and b* values decreased as RD time increased, regardless of the aging treatment. Wet-aged steaks had higher L* (P < 0.05), a* (P < 0.05), and b* values (P < 0.001) than any other dry-aged treatment. No differences in L*, a*, and b* values among dry aging treatments were found (P < 0.05). A 2-way interaction between treatment and RD for discoloration was observed (P < 0.05). No differences were found among treatments over the first 2 d of RD (P > 0.05). Samples began to diverge on Day 3 of RD. Dry-aged steaks had greater discoloration scores (P < 0.05) than wet-aged steaks at 4, 5, 6, and 7 d of RD. However, no differences in discoloration scores among RH treatments were found. There was a RD effect on TBARS values (P < 0.001). Greater TBARS values were found as RD progressed from d 0 to d 4 and d 7, regardless of the aging treatment. A treatment effect was observed for lipid oxidation (P < 0.05). Dry-aged steaks had higher TBARS values than wet-aged steaks. No differences in TBARS values among dry aging treatments were found.ConclusionDry aging of beef resulted in decreased lightness and redness values and increased lipid oxidation compared to wet aging. Results suggest that with prolonged RD dry aging of beef has the potential to reduce color and lipid stability compared to wet-aging and thus reduce display life. Dry-aged steaks met the 20% discoloration threshold and overcame the acceptability threshold of 2.28 mg of malonaldehyde/kg at d 4 of RD, indicating that dry-aged steaks can be merchandised in the retail level for 3 d without detrimental effects on color and lipid oxidation.


2020 ◽  
Author(s):  
Resti Tito Villarino ◽  
Christopher Arcay ◽  
Maria Concepcion Temblor

BACKGROUND Hypertension is a serious health issue and a major cardiovascular disease and stroke risk factor. In hypertensive patients, various health educational models have been used to improve their lifestyle, but the findings are inconsistent. OBJECTIVE The study assessed the effects of a lifestyle intervention program using modified Beliefs, Attitude, Subjective Standards, Enabling Factors (BASNEF) model among non-adherent hypertensive respondents in relation to the introduction of a lifestyle intervention program in the management of hypertension. METHODS This is a quantitative quasi-experimental research particularly utilizing a repeated-measures design of within-subjects approach on the 50 non-adherent patients diagnosed with essential hypertension at Moalboal, Cebu, Philippines in 2019. The respondents received five sessions of trainings based on modified BASNEF model. The Morisky Medication Adherence instrument was used. The first phase included a demographic questionnaire and the last phase comprised the evaluation of the program. Frequencies, percentages, means and standard deviations for descriptive statistics while t-test, repeated measures, ANOVA, and Pearson product moment correlation for inferential statistics. RESULTS The result indicated that the phase 1 mean (146.5) of the systolic readings differ significantly from the phase 4 mean (134.92) of the systolic readings. However, since these two means came from phases that were not consecutive, the result, as a whole, did not show a significant decrease or change when analyzed chronologically from one phase to the next. CONCLUSIONS The study has established that BASNEF model approach can be an effective BP management technique.


2020 ◽  
pp. 104973152098235
Author(s):  
Kuei-Min Chen ◽  
Hui-Fen Hsu ◽  
Li-Yen Yang ◽  
Chiang-Ching Chang ◽  
Yu-Ming Chen ◽  
...  

Purpose: This study aimed to test the effectiveness of High-Need Community-Dwelling Older Adults Care Delivery Model (HCOACDM) in Taiwan. Methods: A cluster randomized controlled trial with repeated measures design was conducted in eight community care centers, involving 145 high-need older adults who were assigned to the intervention group or comparison group. The HCOACDM was provided over 6 months. Functional ability, quality of life, depressive symptoms, and health care and social service utilizations were measured at baseline, at 3 months, and 6 months into the intervention. The participants’ satisfaction was measured at the end of 6-month intervention. Results: Positive effects were shown on all variables in the intervention group at both the 3-month and 6-month intervals (all p < .05). The intervention group had a higher satisfaction with care delivery than the comparison group ( p < .05). Discussion: The promising findings supported a long-term implementation of the HCOACDM as applicable and beneficial.


Author(s):  
Nuno Batalha ◽  
Jose A. Parraca ◽  
Daniel A. Marinho ◽  
Ana Conceição ◽  
Hugo Louro ◽  
...  

The purpose of this study was to analyze the acute effects of a standardized water training session on the shoulder rotators strength and balance in age group swimmers, in order to understand whether a muscle-strengthening workout immediately after the water training is appropriate. A repeated measures design was implemented with two measurements performed before and after a standardized swim session. 127 participants were assembled in male (n = 72; age: 16.28 ± 1.55 years, height: 174.15 ± 7.89 cm, weight: 63.97 ± 6.51 kg) and female (n = 55; age: 15.29 ± 1.28 years, height: 163.03 ± 7.19 cm, weight: 52.72 ± 5.48 kg) cohorts. The isometric torque of the shoulder internal (IR) and external (ER) rotators, as well as the ER/IR ratios, were assessed using a hand-held dynamometer. Paired sample t-tests and effect sizes (Cohen’s d) were used (p ≤ 0.05). No significant differences were found on the shoulder rotators strength or balance in males after training. Females exhibited unchanged strength values after practice, but there was a considerable decrease in the shoulder rotators balance of the non-dominant limb (p < 0.01 d = 0.366). This indicates that a single practice seems not to affect the shoulders strength and balance of adolescent swimmers, but this can be a gender specific phenomenon. While muscle-strengthening workout after the water session may be appropriate for males, it can be questionable regarding females. Swimming coaches should regularly assess shoulder strength levels in order to individually identify swimmers who may or may not be able to practice muscle strengthening after the water training.


2017 ◽  
Vol 3 (3) ◽  
pp. 88-93 ◽  
Author(s):  
Maureen Anne Jersby ◽  
Paul Van-Schaik ◽  
Stephen Green ◽  
Lili Nacheva-Skopalik

BackgroundHigh-Fidelity Simulation (HFS) has great potential to improve decision-making in clinical practice. Previous studies have found HFS promotes self-confidence, but its effectiveness in clinical practice has not been established. The aim of this research is to establish if HFS facilitates learning that informs decision-making skills in clinical practice using MultipleCriteria DecisionMaking Theory (MCDMT).MethodsThe sample was 2nd year undergraduate pre-registration adult nursing students.MCDMT was used to measure the students’ experience of HFS and how it developed their clinical decision-making skills. MCDMT requires characteristic measurements which for the learning experience were based on five factors that underpin successful learning, and for clinical decision-making, an analytical framework was used. The study used a repeated-measures design to take two measurements: the first one after the first simulation experience and the second one after clinical placement. Baseline measurements were obtained from academics. Data were analysed using the MCDMT tool.ResultsAfter their initial exposure to simulation learning, students reported that HFS provides a high-quality learning experience (87%) and supports all aspects of clinical decision-making (85%). Following clinical practice, the level of support for clinical decision-making remained at 85%, suggesting that students believe HFS promotes transferability of knowledge to the practice setting.ConclusionOverall, students report a high level of support for learning and developing clinical decision-making skills from HFS. However, there are no comparative data available from classroom teaching of similar content so it cannot be established if these results are due to HFS alone.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yanzhi Bi ◽  
Xin Hou ◽  
Jiahui Zhong ◽  
Li Hu

AbstractPain perception is a subjective experience and highly variable across time. Brain responses evoked by nociceptive stimuli are highly associated with pain perception and also showed considerable variability. To date, the test–retest reliability of laser-evoked pain perception and its associated brain responses across sessions remain unclear. Here, an experiment with a within-subject repeated-measures design was performed in 22 healthy volunteers. Radiant-heat laser stimuli were delivered on subjects’ left-hand dorsum in two sessions separated by 1–5 days. We observed that laser-evoked pain perception was significantly declined across sessions, coupled with decreased brain responses in the bilateral primary somatosensory cortex (S1), right primary motor cortex, supplementary motor area, and middle cingulate cortex. Intraclass correlation coefficients between the two sessions showed “fair” to “moderate” test–retest reliability for pain perception and brain responses. Additionally, we observed lower resting-state brain activity in the right S1 and lower resting-state functional connectivity between right S1 and dorsolateral prefrontal cortex in the second session than the first session. Altogether, being possibly influenced by changes of baseline mental state, laser-evoked pain perception and brain responses showed considerable across-session variability. This phenomenon should be considered when designing experiments for laboratory studies and evaluating pain abnormalities in clinical practice.


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