scholarly journals First Report of Cowpea Aphid-Borne Mosaic Potyvirus from Cowpeas Grown Commercially in the U.S.

Plant Disease ◽  
1997 ◽  
Vol 81 (8) ◽  
pp. 959-959 ◽  
Author(s):  
A. S. Kline ◽  
E. J. Anderson
Keyword(s):  
Chenopodium Quinoa ◽  
The United States ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mechanical Transmission ◽  
First Report ◽  
Chlorotic Mottle ◽  
Cowpea Aphid ◽  
Healthy Control ◽  
Systemic Infections ◽  
The U.S

Cowpea aphid-borne mosaic potyvirus (CABMV) is one of several seed-borne viruses known to limit cowpea (Vigna unguiculata (L.) Walp. subsp. unguiculata) production in Africa, Europe, and Asia, but CABMV has not been reported on commercially grown cowpeas in the United States (1). However, a sesame (Sesamum indicum L.)-infecting isolate of CABMV was recently characterized from plants growing near cowpea introduction plots in Georgia (2). In February 1997, we received samples of three seed lots of cowpea cv. Chinese Red that had been harvested in southern Texas during 1996. Approximately 28% of the plants grown from these seed lots expressed strong mosaic symptoms on primary and trifoliate leaves. Viruslike symptoms were reproduced following mechanical transmission to plants of Chinese Red cowpea, Nicotiana benthamiana, and soybean (Glycine max L.) cv. Lee. When Coronet and Pinkeye Purple Hull-BVR cowpeas were inoculated with sap extracts from symptomatic Chinese Red plants, chlorotic lesions developed on inoculated leaves, but only Coronet plants supported symptomless systemic infections. Similarly inoculated plants of Chenopodium quinoa (L.) and common bean (Phaseolus vulgaris L.) cvs. Pinto and Black Valentine developed localized chlorotic lesions. In Ouchterlony gel diffusion assays, extracts from symptomatic cowpea plants did not react with antisera to blackeye cowpea mosaic potyvirus (BlCMV), cucumber mosaic cucu-movirus (CMV), southern bean mosaic sobemovirus, cowpea mosaic comovirus, cowpea severe mosaic comovirus, or cowpea chlorotic mottle bromovirus. In the indirect enzyme-linked immunosorbent assay, sap extracts from symptomatic plants reacted with antiserum to CABMV, giving OD values at A405 of 0.10 to 0.25, and reacted weakly with antiserum to BlCMV, with OD values at A405 less than 0.035. Extracts from healthy control plants gave OD values at A405 less than 0.010. No positive reactions were obtained with antisera to bean yellow mosaic potyvirus, peanut mottle potyvirus, soybean mosaic potyvirus, or CMV. To our knowledge, this is the first report of CABMV in commercially grown cowpea from the U.S. References: (1) A. G. Gillaspie et al. Plant Dis. 79:388, 1995. (2) H. R. Pappu et al. Arch. Virol. 142:1, 1997.

scholarly journals First Report of Turnip mosaic virus in Pisum sativum in Spain

Plant Disease ◽  
2003 ◽  
Vol 87 (1) ◽  
pp. 103-103 ◽  
Author(s):  
E. Segundo ◽  
G. Martín-Bretones ◽  
L. Ruiz ◽  
L. Velasco ◽  
D. Janssen ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Pisum Sativum ◽  
Mosaic Virus ◽  
Chenopodium Quinoa ◽  
The United States ◽  
Amino Acid Identity ◽  
Turnip Mosaic Virus ◽  
First Report ◽  
Flower Abortion ◽  
Chlorotic Mottle

During 2001 and 2002, Pisum sativum var. vulgare plants grown as commercial crops in Almeria (southeast Spain) showed vein clearing and chlorotic mottle of leaves, leaf deformation, flower abortion, necrotic mottle and deformation of pods, and stunted plant growth. Crude sap of collected plants was mechanically inoculated on healthy pea plants which reproduced symptoms observed in the field; local necrotic lesions were produced on mechanically infected Chenopodium quinoa, C. amaranticolor, and Gomphrena globosa, systemic mosaic symptoms on Brassica napus and Nicotiana benthamiana, and local lesions plus systemic mosaic symptoms on N. clevelandii, which are all characteristic of Turnip mosaic virus (TuMV) (1). A reverse transcription-polymerase chain reaction assay using general primers for the extreme 3′ end of the potyvirus genome amplified products of 750 and 1,700 bp in nucleic acid extracts from naturally infected pea plants as well as from the mechanically infected test plants. The overlapping nucleotide sequences of the products (GenBank Accession No. AJ489259) had a nucleotide sequence identity of 86.5% and a derived amino acid identity of 95.0% with several published sequences of TuMV (1). This report cites the first partial nucleotide sequence of TuMV infecting pea crops, and although natural infections of this virus in pea have been reported in Morocco (1976) and in the United States (2), to our knowledge, this is the first report of TuMV in Spain. References: (1) P. Lehmann et al. Physiol. Mol. Plant Pathol. 51:195, 1997. (2) R. Provvidenti. Plant Dis. Rep. 62:482, 1978.

scholarly journals First Report of Xylella fastidiosa Associated with Leaf Scorch in Black Oak in Washington, D.C.

Plant Disease ◽  
2004 ◽  
Vol 88 (2) ◽  
pp. 224-224 ◽  
Author(s):  
Q. Huang
Keyword(s):  
Xylella Fastidiosa ◽  
The United States ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bacterial Suspension ◽  
White Oak ◽  
First Report ◽  
Leaf Petiole ◽  
Leaf Scorch ◽  
Pcr Product ◽  
Black Oak

Bacterial leaf scorch caused by Xylella fastidiosa has been reported in 17 species of oak including bur, pin, red, scarlet, shingle, and white oaks (3). In September 2002, a leaf scorch symptom characterized by marginal necrosis of leaves bordered by a darker brown band was observed in a mature black oak (Quercus velutina Lam.) at the U.S. National Arboretum in Washington, D.C. The leaf petiole of the black oak was processed in general extraction buffer (Agdia, Inc., Elkhart, IN) contained in a FastDNA lysing matrix tube using the FastPrep FP120 instrument (Qbiogene, Inc., Carlsbad, CA) (1). The leaf petiole extract reacted with an antiserum specific for X. fastidiosa (Agadia, Inc.) in an enzyme-linked immunosorbent assay (ELISA). A slow-growing bacterium was cultured from leaf petioles of the affected black oak tree by soaking the surface-sterilized, finely cut leaf petioles in sterile water for 30 min, followed by spreading the bacterial suspension on periwinkle wilt plates (1). When the cultured bacterium was subjected to polymerase chain reaction (PCR) with primers specific for X. fastidiosa (2), a 472-bp PCR product was detected. The PCR product was confirmed to be the predicted X. fastidiosa product by sequencing and sequence comparison with the reported genomic sequence of X. fastidiosa. ELISA and bacterial isolation from leaf petioles of a nearby symptomless white oak (Q. alba L.) tree were negative. To our knowledge, this is the first report of X. fastidiosa associated with leaf scorch in black oak in the United States, expanding the host range of the bacterium in economically important landscape tree species. References: (1) Q. Huang and J. L. Sherald. Curr. Microbiol. 48:73, 2004. (2) M. R. Pooler and J. S. Hartung. Curr. Microbiol. 31:377, 1995. (3) J. L. Sherald. Xylella fastidiosa, A bacterial pathogen of landscape trees. Page 191 in: Shade Tree Wilt Diseases, C. L. Ash, ed. The American Phytopathological Society, 2001.

scholarly journals First Report of Cucurbit yellow stunting disorder virus in Morocco

Plant Disease ◽  
2000 ◽  
Vol 84 (5) ◽  
pp. 596-596 ◽  
Author(s):  
C. Desbiez ◽  
H. Lecoq ◽  
S. Aboulama ◽  
M. Peterschmitt
Keyword(s):  
Important Change ◽  
Enzyme Linked Immunosorbent Assay ◽  
Yellow Leaf ◽  
Vegetable Crops ◽  
Specific Primers ◽  
First Report ◽  
Large Populations ◽  
Healthy Control ◽  
Symptomless Plant ◽  
Beet Pseudo Yellows Virus

In October, 1999, severe yellowing symptoms were observed in a melon (Cucumis melo L.) crop grown under plastic tunnels in the region of Agadir, Morocco. Large populations of whiteflies (Bemisia tabaci) were noticed during the early stages of the crop. At harvest, leaf samples were collected from two symptomatic plants and one symptomless plant. A mature yellow leaf was assayed from each symptomatic plant and for one of these two plants a younger leaf exhibiting only yellow spots. Cucurbit aphid-borne yellows virus, which causes similar symptoms in melons, was not detected by double-antibody sandwich enzyme-linked immunosorbent assay tests. Total RNA was extracted from fresh leaf tissues and submitted to reverse transcription and polymerase chain reaction with primers specific to two whitefly-transmissible viruses: Beet pseudo-yellows virus (BPYV) and Cucurbit yellow stunting disorder virus (CYSDV) (2). No amplification was obtained with BPYV-specific primers. In contrast, an expected 465-bp product was amplified in all samples from symptomatic plants with CYSDV-specific primers. No amplification was detected in samples from the symptomless plant nor from healthy control plants. B. tabaci-transmitted CYSDV has been reported in the Middle East, southwestern Europe, and North America (1,4). This is the first report of CYSDV in Morocco, and it follows the first report of another B. tabaci-transmitted virus, Tomato yellow leaf curl virus, in tomato (3), suggesting an important change in the viral pathosystem affecting vegetable crops in Morocco. References: (1) Kao et al. Plant Dis. 84:101, 2000. (2) Livieratos et al. Plant Pathol. 47:362, 1998. (3) Peterschmitt et al. Plant Dis. 83:1074, 1999. (4) Wisler et al. Plant Dis. 82:270, 1998.

scholarly journals First Report of Grapevine leafroll associated virus-3 in American Vitis spp. Grapevines in Washington State

Plant Disease ◽  
2006 ◽  
Vol 90 (11) ◽  
pp. 1461-1461 ◽  
Author(s):  
M. J. Soule ◽  
K. C. Eastwell ◽  
R. A. Naidu
Keyword(s):  
New York ◽  
Economic Impact ◽  
Virus Disease ◽  
The United States ◽  
Washington State ◽  
The State ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rt Pcr ◽  
First Report ◽  
Table Grapes

Washington State is the largest producer of juice grapes (Vitis labruscana ‘Concord’ and Vitis labrusca ‘Niagara’) and ranks second in wine grape production in the United States. Grapevine leafroll disease (GLD) is the most wide spread and economically significant virus disease in wine grapes in the state. Previous studies (2) have shown that Grapevine leafroll associated virus-3 (GLRaV-3) is the predominant virus associated with GLD. However, little is known about the incidence and economic impact of GLD on juice and table grapes. Because typical GLD symptoms may not be obvious among these cultivars, the prevalence and economic impact of GLD in Concord and Niagara, the most widely planted cultivars in Washington State, has received little attention from the grape and nursery industries. During the 2005 growing season, 32 samples from three vineyards and one nursery of ‘Concord’ and three samples from one nursery of ‘Niagara’ were collected randomly. Petiole extracts were tested by single-tube reverse transcription-polymerase chain reaction (RT-PCR; 3) with primers LC 1 (5′-CGC TAG GGC TGT GGA AGT ATT-3′) and LC 2 (5′-GTT GTC CCG GGT ACC AGA TAT-3′), specific for the heat shock protein 70 homologue (Hsp70h gene) of GLRaV-3 (GenBank Accession No. AF037268). One ‘Niagara’ nursery sample and eleven ‘Concord’ samples from the three vineyards tested positive for GLRaV-3, producing a single band of the expected size of 546 bp. The ‘Niagara’ and six of the ‘Concord’ RT-PCR products were cloned in pCR2.1 (Invitrogen Corp, Carlsbad, CA) and the sequences (GenBank Accession Nos. DQ780885, DQ780886, DQ780887, DQ780888, DQ780889, DQ780890, and DQ780891) compared with the respective sequence of a New York isolate of GLRaV-3 (GenBank Accession No. AF037268). The analysis revealed that GLRaV-3 isolates from ‘Concord’ and ‘Niagara’ share nucleotide identities of 94 to 98% and amino acid identities and similarities of 97 to 98% with the Hsp70h gene homologue of the New York isolate of GLRaV-3. Additional testing by double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) using antibodies specific to GLRaV-3 (BIOREBA AG, Reinach, Switzerland) further confirmed these results in the ‘Niagara’ and two of the ‘Concord’ isolates. GLRaV-3 has previously been reported in labrusca cvs. Concord and Niagara in western New York (4) and Canada (1), but to our knowledge, this is the first report of GLRaV-3 in American grapevine species in the Pacific Northwest. Because wine and juice grapes are widely grown in proximity to each other in Washington State and grape mealybug (Pseudococcus maritimus), the putative vector of GLRaV-3, is present in the state vineyards, further studies will focus on the role of American grapevine species in the epidemiology of GLD. References: (1) D. J. MacKenzie et al. Plant Dis. 80:955, 1996. (2) R. R. Martin et al. Plant Dis. 89:763, 2005. (3) A. Rowhani et al. ICGV, Extended Abstracts, 13:148, 2000. (4) W. F. Wilcox et al. Plant Dis. 82:1062, 1998.

scholarly journals First Report of Plum pox virus (Sharka Disease) in Prunus persica in the United States

Plant Disease ◽  
2000 ◽  
Vol 84 (2) ◽  
pp. 202-202 ◽  
Author(s):  
L. Levy ◽  
V. Damsteegt ◽  
R. Welliver
Keyword(s):  
Prunus Persica ◽  
Virus Disease ◽  
Sandwich Elisa ◽  
Pcr Amplification ◽  
The United States ◽  
Enzyme Linked Immunosorbent Assay ◽  
Plum Pox Virus ◽  
Rt Pcr ◽  
Peach Fruit ◽  
First Report

Plum pox (Sharka) is the most important virus disease of Prunus in Europe and the Mediterranean region and is caused by Plum pox potyvirus (PPV). In September 1999, PPV-like symptoms were observed in peach fruit culls in a packinghouse in Pennsylvania. All symptomatic fruit originated from a single block of peach (P. persica cv. Encore) in Adams County. Trees in the block exhibited ring pattern symptoms on their leaves. A potyvirus was detected in symptomatic fruit using the Poty-Group enzyme-linked immunosorbent assay (ELISA) test from Agdia (Elkhart, IN). Reactions for symptomatic peach fruit and leaves also were positive using triple-antibody sandwich ELISA with the PPV polyclonal antibody from Bioreba (Carrboro, NC) for coating, the Poty-Group monoclonal antibody (MAb; Agdia) as the intermediate antibody, and double-antibody sandwich ELISA with PPV detection kits from Sanofi (Sanofi Diagnostics Pasteur, Marnes-La-Coquette, France) and Agdia and the REAL PPV kit (Durviz, Valencia, Spain) containing universal (5B) and strain typing (4DG5 and AL) PPV MAbs (1). PPV also was identified by immunocapture-reverse transcription-polymerase chain reaction (IC-RT-PCR) amplification and subsequent sequencing of the 220-bp 3′ noncoding region (2) (>99% sequence homology to PPV) and by IC-RT-PCR amplification of a 243-bp product in the coat protein (CP) gene (1). The virus was identified as PPV strain D based on serological typing with strainspecific MAbs and on PCR-restriction fragment length polymorphism of the CP IC-RT-PCR product with Rsa1 and Alu1 (1). This is the first report of PPV in North America. References: (1) T. Candresse et al. Phytopathology 88:198, 1998. (2) L. Levy and A. Hadidi. EPPO Bull. 24:595, 1994.

scholarly journals First Report of Beet necrotic yellow vein virus Infecting Spinach in California

Plant Disease ◽  
2010 ◽  
Vol 94 (5) ◽  
pp. 640-640 ◽  
Author(s):  
H.-Y. Liu ◽  
B. Mou ◽  
K. Richardson ◽  
S. T. Koike
Keyword(s):  
Spinacia Oleracea ◽  
Sandwich Elisa ◽  
Chenopodium Quinoa ◽  
Yellow Vein ◽  
Mechanical Transmission ◽  
Polymyxa Betae ◽  
Rt Pcr ◽  
First Report ◽  
Vein Clearing ◽  
Rigid Rod

In 2009, plants from two spinach (Spinacia oleracea) experimental fields in Monterey County and one commercial spinach field in Ventura County of California exhibited vein-clearing, mottling, interveinal yellowing, and stunting symptoms. For experimental fields, up to 44% of spinach plants have symptoms. With a transmission electron microscope, rigid rod-shaped particles with central canals were observed from plant sap of the symptomatic spinach. Analysis with a double-antibody sandwich-ELISA assay for Beet necrotic yellow vein virus (BNYVV) showed that all 10 symptomatic plants we tested were positive and 5 asymptomatic plants were negative. Symptomatic spinach from both counties was used for mechanical transmission experiments. Chenopodium quinoa, Tetragonia expansa, and Beta vulgaris (sugar beet) showed chlorotic local lesions and B. macrocarpa and spinach showed vein-clearing, mottling, and systemic infections. To further confirm the presence of BNYVV, reverse transcription (RT)-PCR was conducted. Total RNA was extracted from field- and mechanically inoculated symptomatic spinach plants using an RNeasy Plant Kit (Qiagen Inc., Valencia, CA) and used as a template in RT-PCR. Forward and reverse primers specific to the BNYVV RNA-3 P25 protein gene from the beet isolate were used (2). Amplicons of the expected size (approximately 860 bp) were obtained. Four RT-PCR products were sequenced and the sequences were identical (GenBank Accession No. GU135626). Sequences from the spinach plants had 97 to 99% nucleotide and 94 to 100% amino acid identity with BNYVV RNA-3 P25 protein sequences available in the GenBank. On the basis of the data from electron microscopy, indicator plants, serology, and cDNA sequencing, the virus was identified as BNYVV. BNYVV has been reported from spinach fields in Italy (1). To our knowledge, this is the first report of BNYVV occurring naturally on spinach in California. Since BNYVV is transmitted by the zoospores of the soil-inhabiting plasmodiophorid Polymyxa betae, it could be a new threat to spinach production in the state. References: (1) C. R. Autonell et al. Inf. Fitopatol. 45:43, 1995. (2) H.-Y. Liu and R. T. Lewellen, Plant Dis. 91:847, 2007.

scholarly journals First Report on Natural Occurrence of Tomato Spotted Wilt Tospovirus in Basil (Ocimum basilicum)

Plant Disease ◽  
1999 ◽  
Vol 83 (10) ◽  
pp. 966-966 ◽  
Author(s):  
G. E. Holcomb ◽  
R. A. Valverde ◽  
J. Sim ◽  
J. Nuss
Keyword(s):  
Plant Viruses ◽  
Ocimum Basilicum ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mechanical Transmission ◽  
Summer Drought ◽  
Natural Occurrence ◽  
Thin Sections ◽  
First Report ◽  
Tomato Spotted Wilt ◽  
National University

Virus-like symptoms were observed on basil plants (Ocimum basilicum L. ‘Mrs. Burns Lemon’ [MBL]) growing in containers and a demonstration plot at the Louisiana State University Burden Research Plantation, Baton Rouge, during July 1998. Symptoms consisted of ring spots, leaf distortion, and severe mosaic. Mechanical transmission of the suspect virus by sap inoculation from infected MBL to basil cvs. MBL, Aussie Sweet, Cinnamon, Siam Queen, and Sweet Dani was successful. Symptoms were similar to those on infected MBL. Nicotiana benthamiana Domin. reacted with local chlorotic spots followed by severe yellows, necrosis, and death. Electron microscopy of thin sections of infected basil revealed virus inclusions but no virus particles. However, infected N. benthamiana revealed the presence of 82-nm membrane-bound particles in the cytoplasm. The virus was identified from basil and N. benthamiana as the common strain of tomato spotted wilt tospovirus (TSWV) by enzyme-linked immunosorbent assay (Agdia, Elkhart, IN). An outbreak of thrips insects during the summer drought in 1998 was probably responsible for the occurrence of TSWV in basil. This is the first report of the occurrence of TSWV in basil (1). Reference: (1) A. A. Brunt et al., eds. 1996. Plant Viruses Online: Descriptions and Lists from the VIDE Database. Published online by Australian National University, Canberra.

scholarly journals First Report of Tomato spotted wilt virus on Soybean in Iran

Plant Disease ◽  
2001 ◽  
Vol 85 (12) ◽  
pp. 1290-1290 ◽  
Author(s):  
A. R. Golnaraghi ◽  
N. Shahraeen ◽  
R. Pourrahim ◽  
Sh. Ghorbani ◽  
Sh. Farzadfar
Keyword(s):  
Glycine Max ◽  
Tomato Spotted Wilt Virus ◽  
Chenopodium Quinoa ◽  
Datura Stramonium ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mechanical Inoculation ◽  
First Report ◽  
Tomato Spotted Wilt ◽  
Soybean Leaves ◽  
Wilt Virus

During the summers of 1999 and 2000, 3,110 soybean (Glycine max) leaf samples were randomly collected from soybean fields in the Ardebil, Goletan, Khuzestan, Lorestan, and Mazandaran provinces of Iran. Tomato spotted wilt virus (TSWV) was detected in leaf samples by TSWV-specific polyclonal antibody (As-0526 and As-0580, DSMZ, Braunschweig, Germany) in double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). Mechanical inoculation of 26 plant species (10 plants per species) and cultivars with extracts of positive leaf samples produced necrotic local lesions in Beta vulgaris, Chenopodium quinoa, C. amaranticolor, Gomphrena globosa, Phaseolus vulgaris cv. Talash, Vicia faba, and Vigna unguiculata cv. Mashad; produced systemic chlorosis followed by necrosis in Datura stramonium, D. metel, Nicotiana rustica, N. tabacum cv. Samsun, N. glutinosa, N. bentamiana, and Glycine max cv. Hill; and produced chlorosis, stunting, and bud necrosis in Arachis hypogaea (peanut). Plants developing these symptoms following mechanical inoculation with extracts from original soybean leaves were positive in ELISA for TSWV. ELISA results indicate that the overall incidence of TSWV on soybean in the five provinces was 5.4%. TSWV has been reported in potato (2) and tomato (1) from Iran, but to our knowledge, this is the first report of the occurrence of TSWV on soybean in Iran. References: (1) K. Bananej et al. Iran. J. Plant Pathol. 34:30, 1998. (2) R. Pourrahim et al. Plant Dis. 85:442, 2001.

scholarly journals Smoking-Attributable Mortality by Cause of Death in the United States: An Indirect Approach

2019 ◽  
Author(s):  
Joseph T. Lariscy
Keyword(s):  
United States ◽  
Lung Cancer ◽  
Causes Of Death ◽  
Smoking Status ◽  
The United States ◽  
Current Evidence ◽  
Attributable Mortality ◽  
Death Rates ◽  
First Report ◽  
The U.S

More than 50 years after the U.S. Surgeon General's first report on cigarette smoking and mortality, smoking remains the leading cause of preventable death in the United States. The first report established a causal association between smoking and lung cancer, and subsequent reports expanded the list of smoking-attributable causes of death to include other cancers, cardiovascular diseases, stroke, and respiratory diseases. For a second level of causes of death, the current evidence is suggestive but not sufficient to infer a causal relationship with smoking. This study draws on 1980–2004 U.S. vital statistics data and applies a cause-specific version of the Preston-Glei-Wilmoth indirect method, which uses the association between lung cancer death rates and death rates for other causes of death to estimate the fraction and number of deaths attributable to smoking overall and by cause. Nearly all of the established and additional causes of death are positively associated with lung cancer mortality, suggesting that the additional causes are in fact attributable to smoking. I find 420,284 annual smoking-attributable deaths at ages 50+ for years 2000–2004, 14% of which are due to the additional causes. Results corroborate recent estimates of cause-specific smoking-attributable mortality using prospective cohort data that directly measure smoking status. The U.S. Surgeon General should reevaluate the evidence for the additional causes and consider reclassifying them as causally attributable to smoking.

scholarly journals First Report of Beet soilborne virus in Poland

Plant Disease ◽  
2006 ◽  
Vol 90 (1) ◽  
pp. 112-112 ◽  
Author(s):  
N. Borodynko ◽  
B. Hasiów ◽  
H. Pospieszny
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Sugar Beet ◽  
Amino Acid Sequence Identity ◽  
Chenopodium Quinoa ◽  
Enzyme Linked Immunosorbent Assay ◽  
First Report ◽  
Sequence Identity ◽  
Pcr Products ◽  
Soilborne Viruses

Beet necrotic yellow vein virus (BNYVV), the casual agent of rhizomania disease, was identified in sugar beet plants from several fields in the Wielkopolska Region of Poland (1). In greenhouse studies, sugar beets were grown in the soil from one of these fields to bait soilborne viruses. Of 200 sugar beet plants, three developed symptoms of vein clearing, vein banding, and mosaic. Crude sap from symptomatic plants was used for mechanical inoculation of various plants species. In Chenopodium quinoa, C. amaranticolor, and Tetragonia expansa only local lesions were observed. Electron microscope examination of negatively stained leaf-dip preparations from symptomatic sugar beet plants showed a mixture of rod-shape particles from 70 to 400 nm long. Using double-antibody sandwich enzyme-linked immunosorbent assay tests, two symptomatic sugar beet plants gave positive reactions with antiserum against BNYVV (Bio-Rad, Hercules, CA) and a third plant gave a positive reaction with antisera against BNYVV and Beet soilborne virus (BSBV). Total RNA was extracted from roots and leaves of the symptomatic plants and used in a multiplex reverse transcription-polymerase chain reaction (mRT-PCR) assay. Specific primers were designed to amplify a fragment of the RNA1 for BSBV and RNA2 for BNYVV and Beet virus Q (BVQ) (2). Two mRT-PCR products amplified with the primers specific to BNYVV and BSBV were obtained and sequenced. A 274-nt amplicon sequence (GenBank Accession No. DQ012156) had 98% nucleotide sequence identity with the German BNYVV isolate F75 (GenBank Accession No. AF19754) and a 376-nt amplicon sequence (GenBank Accession No. AY999690) had 98% nucleotide and 98% amino acid sequence identity with the German BSBV isolate (GenBank Accession No. Z97873). The Polish BSBV isolate had 88% nucleotide and 62% amino acid sequence identity with BVQ, another pomovirus (GenBank Accession No. AJ 223596 formerly known as serotype Wierthe of BSBV (2). In 2005, mRT-PCR was used on samples collected from two fields of the Wielkopolska Region. Of 15 tested sugar beet plants, 12 gave positive reactions with primers specific for BSBV and nine with primers specific to BNYVV. To our knowledge, this is first report of BSBV in Poland. In Europe, BSBV was previously reported in England, the Netherlands, Belgium, Sweden, Germany, France, and Finland (2,3). References: (1) M. Jezewska and J. Piszczek. Phytopathol. Polonica, 21:165, 2001. (2) A. Maunier et al. Appl. Environ. Microbiol. 69:2356, 2003. (3) C. M. Rush and G. B. Heidel. Plant Dis. 79:868, 1995.

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