Advanced technology in the hospital laboratory: Equipment for clinical chemistry and its evaluation

1973 ◽  
Vol 184 (1077) ◽  
pp. 351-359 ◽  

The last 20 years have seen the introduction of automation into clinical chemistry on a large scale and much of the once familiar equipment - burettes, pipettes, test tubes etc., have largely disappeared. Manual error can be minimized and complex procedures for quality control successfully applied. A wide range of instruments are now available from simple dispensers and diluters to complex, fast, computer controlled twenty channel analysers producing twenty results every 12 s. The value of the most expensive item of equipment in the laboratory has increased from £30 to £100 000 and it is important that expensive items are fully evaluated for cost effectiveness and efficiency so that the right purchase is made for each situation.

F1000Research ◽  
2021 ◽  
Vol 10 ◽  
pp. 581
Author(s):  
Pavel Kodytek ◽  
Alexandra Bodzas ◽  
Petr Bilik

The wood industry is facing many challenges. The high variability of raw material and the complexity of manufacturing processes results in a wide range of visible structure defects, which have to be controlled by trained specialists. These manual processes are not only tedious and biased, but also less effective. To overcome the drawbacks of the manual quality control processes, several automated vision-based systems have been proposed. Even though some conducted studies achieved a higher recognition rate than trained experts, researchers have to deal with a lack of large-scale databases and authentic data in this field. To address this issue, we performed a data acquisition experiment set in the industrial environment, where we were able to acquire an extensive set of authentic data from a production line. For this purpose, we designed and implemented a complex technical solution suitable for high-speed acquisition during harsh manufacturing conditions. In this data note, we present a large-scale dataset of high-resolution sawn timber surface images containing more than 43 000 labelled surface defects and covering 10 types of the most common wood defects. Moreover, with each image record, we provide two types of labels allowing researchers to perform semantic segmentation, as well as defect classification, and localization.


Author(s):  
Mary Kay Gugerty ◽  
Dean Karlan

Deworm the World serves millions of school children every year. Monitoring on such a large scale can amplify the difficulty of developing a right-fit system: How can an organization ensure credible data collection across a wide range of sites and prioritize actionable information that informs implementation? How can such a large-scale system rapidly respond to issues once identified? This case illustrates the challenge of finding credible and actionable activity tracking measures. How does Deworm the World apply the credible, actionable, and responsible principles to determine the right amount of data to collect and the right time and place at which to collect it?


2020 ◽  
Vol 34 (5) ◽  
pp. 790-817
Author(s):  
Charlotte Wagnsson ◽  
Eva-Karin Olsson ◽  
Isabella Nilsen

Gender differences have been observed regarding many political and social issues, yet we lack comprehensive evidence on differences in perceptions on a wide range of security issues increasingly important to voters: military threats, criminality, and terrorism. Previous research suggests that when women are highly politically mobilized, as they are in Sweden, gender differences in political opinion are large. On the other hand, Swedish politicians have worked hard to reduce gender stereotypical thinking. This prompts the question: Are there gender differences in attitudes on security issues in Sweden, and if so, in what ways do the attitudes differ? This study is based on comprehensive data from focus groups and a large-scale survey. The results show that women were more prone to respond with an “ethic of care,” across security issues. Women were more inclined to understand security problems as structural, explained by macho culture, segregation, and injustice. Women tend to support preventive measures that provide individuals with opportunities to choose “the right path,” such as education and economic investment in deprived areas. When asked about national security, women believe more in diplomacy and dialogue. In general, women are less inclined to support various repressive solutions.


2021 ◽  
Vol 11 ◽  
Author(s):  
Mariangela Arca ◽  
Tristan Mary-Huard ◽  
Brigitte Gouesnard ◽  
Aurélie Bérard ◽  
Cyril Bauland ◽  
...  

Genebanks harbor original landraces carrying many original favorable alleles for mitigating biotic and abiotic stresses. Their genetic diversity remains, however, poorly characterized due to their large within genetic diversity. We developed a high-throughput, cheap and labor saving DNA bulk approach based on single-nucleotide polymorphism (SNP) Illumina Infinium HD array to genotype landraces. Samples were gathered for each landrace by mixing equal weights from young leaves, from which DNA was extracted. We then estimated allelic frequencies in each DNA bulk based on fluorescent intensity ratio (FIR) between two alleles at each SNP using a two step-approach. We first tested either whether the DNA bulk was monomorphic or polymorphic according to the two FIR distributions of individuals homozygous for allele A or B, respectively. If the DNA bulk was polymorphic, we estimated its allelic frequency by using a predictive equation calibrated on FIR from DNA bulks with known allelic frequencies. Our approach: (i) gives accurate allelic frequency estimations that are highly reproducible across laboratories, (ii) protects against false detection of allele fixation within landraces. We estimated allelic frequencies of 23,412 SNPs in 156 landraces representing American and European maize diversity. Modified Roger’s genetic Distance between 156 landraces estimated from 23,412 SNPs and 17 simple sequence repeats using the same DNA bulks were highly correlated, suggesting that the ascertainment bias is low. Our approach is affordable, easy to implement and does not require specific bioinformatics support and laboratory equipment, and therefore should be highly relevant for large-scale characterization of genebanks for a wide range of species.


Author(s):  
Mariangela Arca ◽  
Tristan Mary-Huard ◽  
Brigitte Gouesnard ◽  
Aurélie Bérard ◽  
Cyril Bauland ◽  
...  

ABSTRACTGenebanks harbor original landraces carrying many original favorable alleles for mitigating biotic and abiotic stresses. Their genetic diversity remains however poorly characterized due to their large within genetic diversity. We developed a high-throughput, cheap and labor saving DNA bulk approach based on SNP Illumina Infinium HD array to genotype landraces. Samples were gathered for each landrace by mixing equal weights from young leaves, from which DNA was extracted. We then estimated allelic frequencies in each DNA bulk based on fluorescent intensity ratio (FIR) between two alleles at each SNP using a two step-approach. We first tested either whether the DNA bulk was monomorphic or polymorphic according to the two FIR distributions of individuals homozygous for allele A or B, respectively. If the DNA bulk was polymorphic, we estimated its allelic frequency by using a predictive equation calibrated on FIR from DNA bulks with known allelic frequencies. Our approach: (i) gives accurate allelic frequency estimations that are highly reproducible across laboratories, (ii) protects against false detection of allele fixation within landraces. We estimated allelic frequencies of 23,412 SNPs in 156 landraces representing American and European maize diversity. Modified Roger’s genetic Distance between 156 landraces estimated from 23,412 SNPs and 17 SSRs using the same DNA bulks were highly correlated, suggesting that the ascertainment bias is low. Our approach is affordable, easy to implement and does not require specific bioinformatics support and laboratory equipment, and therefore should be highly relevant for large-scale characterization of genebanks for a wide range of species.


2019 ◽  
Vol 16 (16) ◽  
pp. 10-25
Author(s):  
Oksana Tsuranova

Background. The modern system of national education, including music, is on the path of reorganization and reformation. Creating new educational models, it is useful to refer to the samples, time-tested, created by people whose names are permanently inscribed in the European cultural and historical fund. This confirms the life and work of Stepan Vasilyevich Smolensky (1848–1909) – teacher, medievalist, composer, regent, reformer of the music education system, public and cultural figure, ideologist of the New Direction of Orthodox Music of the late XIX – first half of the XX centuries. The formation of the ideology of the musician-teacher, the approval of his convictions became possible in many ways thanks to the support of two of his contemporaries, outstanding pedagogical figures – N. Ilminsky and S. Rachinsky. Objectives. The purpose of the article is to reveal the personal interaction of S. Smolensky with N. Ilminsky, S. Rachinsky, to appraise the contribution of the latter to the formation and development of his ideological positions, which determined the direction of further professional activity. Methods. The article uses the method of historicism, which allows us to consider the phenomena of artistic culture, enlightenment and education in the dynamics of their formation. Results. The formation of S. Smolensky took place in the Kazan period of life under the influence of Nikolai Ilminsky and Sergey Rachinsky. Nikolai Ivanovich Ilminsky (1822–1891) – orientologist, mission temissionary-teacher, biblical scholar, takes a special place in the biography of Stepan Vasilyevich Smolensky. The scientific works of N. Ilminsky cover a wide area of knowledge, like that: theology, linguistics, foreign translation, pedagogy and missionary work. His scientific studies, their practical implementation, which have not lost their relevance even nowadays, put Nikolai Ivanovich in a row of prominent figures of the Orthodox enlightenment of small peoples of the Volga region, Ural region and Siberia. The merits of N. Ilmisnky belongs to the founding of the first schools for small nations of the Volga region, as well as the teachers’ seminary in Kazan, where S. Smolensky was invited to the post of teacher of singing, history and geography. Church singing was considered in the missionary policy of the government as an important strategic element of introducing baptized aliens to orthodoxy. To this end, S. Smolensky was involved in a large-scale project of translating religious chants into the languages of the national small peoples of the Volga region, which determined the direction of his entire musical and singing work. Fully sharing the beliefs of N. Ilminsky, the young teacher focused on teaching church singing, in the moral and educational significance of which he infinitely believed. The lack of a methodical program for this discipline in public schools made S. Smolensky delve into this area of knowledge, as a result of which he developed the author’s system of teaching the named subject. In his pedagogical activity, S. Smolensky made extensive use of the methodological manuals created by him, which became an indispensable teaching material for future teachers. Here in Kazan, with the assistance of N. Ilminsky was opened a new page in the life of S. Smolensky, his deep immersion in the field of paleographic research. In Kazan, in the period of close cooperation with N. Ilminsky, typical features of S. Smolensky’s future activity were outlined, which received its brilliant application in the next Moscow period of life, during his leadership and reforming the Synodal School of Church Singing and Choir. S. Smolensky called his last teacher Sergei Alexandrovich Rachinsky (1833–1902) – professor and founder of the Department of Plant Physiology, Moscow University, a teacher, corresponding member of the Imperial St.-Petersburg Academy of Sciences. The acquaintance of S. Smolensky and S. Rachinsky occurred on the basis of the folk soil, based on Orthodox ideals. Foresight of judgment and deep knowledge of ancient church chants gave S. Rachinsky the right to take an active part in the scientific and educational activities of S. Smolensky. This confirms the extensive work carried out by S. Smolensky on the harmonization of the main Orthodox chants, undertaken at the insistence of his elder friend. The reforms carried out by S. Smolensky in Moscow and St.-Petersburg were fundamentally based on the education system of S. Rachinsky, aimed at developing the national element. Conclusions. A powerful monolith in the face of the polyglot and the manager N. Ilminsky, set off by the elegance of the artistic, but at the same time «meekly obstinate» nature of the educator-creator S. Rachinsky multiplied to the personality of Stepan Vasilyevich. In turn, the example of the life and work of S. Smolensky set a high tone and indicated a movement vector for many respectable professionals of musicians, teachers, choir masters, and scientists. Faith S. Smolensky, by lifeblood of the folk song and znamenny chant, inspired a wide range of composers, including P. Chesnokov, A. Kastalsky, S. Rachmaninov, A. Grechaninov, A. Nikolsky, N. Golovanov, K. Shvedov, Vik. Kalinnikov and others. Becoming one of the founders of medievalism in the area of church music, S. Smolensky outlined the main components of a scientific search in the history and theory of ortodox church singing, in the course of which A. Preobrazhensky, A. Nikolsky and others. A gifted teacher and organizer, S. Smolensky showed an example of the work of exemplary musical institutions whose school was attended by the greatest choirmaster of the last century: P. Chesnokov, N. Golovanov, N. Danilin, S. Zharov, A. Egorov and others. What has been said gives the right to assert that we can be fruitful in history, provided, like S. Smolensky, we will with intense effort learn from our forefathers, carefully looking at the value of their professional and life experience.


2020 ◽  
pp. 68-81
Author(s):  
К. О. Хохлова

The tincture is a traditional liquid dosage form for the pharmaceutical market of Ukraine. Today, the range of the tinctures of Ukrainian manufacturers has 21 different items, while only six monographs on different tinctures are included in the State Pharmacopoeia of Ukraine. For standardization of tinctures, different combinations of specific and non-specific physical and chemical methods are used. The high-performance thin-layer chromatography (HPTLC) is a modern and specific chromatographic method, that can be used for the identification of polyphenolic substances responsible for a wide range of pharmacological activities of the tinctures. The purpose of the study was to identify and compare chromatographic fingerprints (images of chromatograms and peak profiles from images) of flavonoids and phenylpropanoids of 10 tinctures of Ukrainian production in the harmonized mobile phases, to select for each tincture the specific marker zones, to determine the suitability of the specified chromatographic conditions for standardization of the tincture and the possibility to perform in these conditions the quality control for several different tinctures in-parallel. The objects of the investigation were Hawthorn tincture, Peppermint tincture, Sage tincture, Motherwort tincture, Calendula tincture, Sophora tincture, Echinacea purpurea tincture, Valerian tincture, Peony tincture, Eucalyptus tincture. The research was carried out using the HPTLC method in the HPTLC automatic herbal system (CAMAG, Switzerland) on the base of two laboratories. For each investigated tincture, the determination and comparison of HPTLC chromatographic fingerprints (images and peak profiles of images) of flavonoids and phenylpropanoids obtained in different harmonized mobile phases were performed. In the harmonized chromatographic conditions the difference in separation of the bioactive substances was determined; the characteristic marker substances of the tinctures were identified; the suitability of the mobile phase for individual analysis of the tincture or in-parallel analysis of different tinctures on the same plate were determined; the possibility to replace one mobile phase by another, if needed, was determined. Proposed HPTLC methods were developed using modern procedure and approach. These allow to reduce of the scope and time of analysis, optimize the economical and labor costs. The findings are of special importance for the pharmaceutical manufacturers and quality control laboratories that perform analysis on a large scale.


Author(s):  
Claudia Condemi ◽  
Loretta Mastroeni ◽  
Pierluigi Vellucci

AbstractStorage hydropower generation plays a crucial role in the electric power system and energy transition because it is the most widespread power generation with low greenhouse gas emissions and, moreover, it is relatively cheap to ramp up and down. As a result, it provides flexibility to the grid and helps mitigate the short-term production uncertainty that affects most green energy technologies. However, using water in reservoirs represents an opportunity cost, which is related to the evolution of plant production capacity and production profitability. As the latter is related to a wide range of types of variables, in order to incorporate it in a large-scale prediction model it is important to select the variables that impact most on storage hydropower generation. In this paper, we investigate the impact of the variables influencing the choices of price maker producers, and, in particular we study the impact of Clean Spark Spread expectations on storage hydroelectric generation. In this connection, using entropy and machine learning tools, we present a method for embedding this expectations in a model to predict storage hydropower generation, showing that, for some time horizon, expectations on CSS have a greater impact than expectations on power prices. It is shown that, if the right mix of power price and CSS expectations is considered, the prediction error of the model is drastically reduced. This implies that it is important to incorporate CSS expectations into the storage hydropower model.


2019 ◽  
Author(s):  
Max Lam ◽  
Swapnil Awasthi ◽  
Hunna J. Watson ◽  
Jackie Goldstein ◽  
Georgia Panagiotaropoulou ◽  
...  

AbstractMotivationGenome-wide association study (GWAS) analyses, at sufficient sample sizes and power, have successfully revealed biological insights for several complex traits. RICOPILI, an open sourced Perl-based pipeline was developed to address the challenges of rapidly processing large scale multi-cohort GWAS studies including quality control, imputation and downstream analyses. The pipeline is computationally efficient with portability to a wide range of high-performance computing (HPC) environments.SummaryRICOPILI was created as the Psychiatric Genomics Consortium (PGC) pipeline for GWAS and has been adopted by other users. The pipeline features i) technical and genomic quality control in case-control and trio cohorts ii) genome-wide phasing and imputation iv) association analysis v) meta-analysis vi) polygenic risk scoring and vii) replication analysis. Notably, a major differentiator from other GWAS pipelines, RICOPILI leverages on automated parallelization and cluster job management approaches for rapid production of imputed genome-wide data. A comprehensive meta-analysis of simulated GWAS data has been incorporated demonstrating each step of the pipeline. This includes all of the associated visualization plots, to allow ease of data interpretation and manuscript preparation. Simulated GWAS datasets are also packaged with the pipeline for user training tutorials and developer work.Availability and ImplementationRICOPILI has a flexible architecture to allow for ongoing development and incorporation of newer available algorithms and is adaptable to various HPC environments (QSUB, BSUB, SLURM and others). Specific links for genomic resources are either directly provided in this paper or via tutorials and external links. The central location hosting scripts and tutorials is found at this URL:https://sites.google.com/a/broadinstitute.org/RICOPILI/[email protected] informationSupplementary data are available.


1981 ◽  
Vol 70 (01) ◽  
pp. 1-10 ◽  
Author(s):  
Peter Fisher

"All substances are poisons; there is none which is not a poison. The right dose differentiates a poison and a remedy."ParacelsusToxicology has made great advances in recent times. Despite this, modern Homœopaths have made less use of it than did their predecessors. Lead poisoning has a long history. An important recent development is the large scale use of lead-containing petrol additives. Some of the biochemical effects of lead are discussed. Lead can cause a wide range of disorders, including psychological abnormalities in children. It can also simulate motor neurone disease and certain porphyrias. The use of lead in homœopathy should be developed on the basis of these findings.


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