Coriander (Coriandrum sativum L.) essential oil: its antibacterial activity and mode of action evaluated by flow cytometry

2011 ◽  
Vol 60 (10) ◽  
pp. 1479-1486 ◽  
Author(s):  
Filomena Silva ◽  
Susana Ferreira ◽  
João A. Queiroz ◽  
Fernanda C. Domingues
Phytomedicine ◽  
2011 ◽  
Vol 19 (1) ◽  
pp. 42-47 ◽  
Author(s):  
Filomena Silva ◽  
Susana Ferreira ◽  
Andreia Duarte ◽  
Dina I. Mendonça ◽  
Fernanda C. Domingues

PLoS ONE ◽  
2014 ◽  
Vol 9 (6) ◽  
pp. e99086 ◽  
Author(s):  
Irlan de Almeida Freires ◽  
Ramiro Mendonça Murata ◽  
Vivian Fernandes Furletti ◽  
Adilson Sartoratto ◽  
Severino Matias de Alencar ◽  
...  

2021 ◽  
Vol 18 (15) ◽  
Author(s):  
Theera SRISAWAT ◽  
Narueparn SUKKASAM ◽  
Jirawadee UPPALA ◽  
Chananchita NILAKE ◽  
Kanokrat KEAWCHAI ◽  
...  

The present study aimed to investigate the activity of Hydnophytum formicarum Jack (Ant Plant) tuber extract against Staphylococcus aureus and Escherichia coli and the mode of action of the extract on bacterial responses. The antibacterial activity was determined using agar well diffusion method. Resazurin-based 96-well microdilution method was used to determine the Minimal Inhibitory Concentrations (MIC) of the extract. Mode of action on bacterial death and response patterns to the extract were assessed by flow cytometry using membrane integrity and granularity profiles. The results, based on serial dilution and zones of inhibition against the bacteria, showed that 2,000 µg/well was potent against S. aureus (16.33±1.53 mm) and E. coli (14.33±0.58 mm). For S. aureus, the MIC concentration was 8,000 µg/mL, while 4,000 µg/mL concentration exhibited activity against E. coli. Flow cytometric profiles confirmed loss of intracellular components, followed by death, for both bacteria. Bacterial responses to the extract had consistent dose- and time-dependent behavior. This is the first mode of action study on bacterial responses to H. formicarum tuber extract. The results suggest that the extract of H. formicarum tuber could serve as a source of an initial active ingredient for developing effective medicines to battle infections caused by pathogens. HIGHLIGHTS Ant plant tuber extract had a strong antibacterial activity against S. aureus and E. coli Intracellular component and bacterial membrane were the remarkable target for action of the extract Dose- and Time-response model for bacterial response to the extract was achieved using Flow cytometry


2010 ◽  
Vol 13 (3) ◽  
pp. 29-38
Author(s):  
Ha Bich Phan ◽  
Thach Ngoc Le

Coriandrum sativum L. belongs to the Apiaceae family, which is cultivated in Dong Nai province, in this paper its seed oil of was studied. The essential oil was extracted by hydrodistillation. We used two methods for activating of hydrodistillation: conventional heating and microwave irradiating. Its physical and chemical indexes were measured. The chemical composition of this oil was identified by GC/MS and quantified by GC/FID. Linalool (75.51-77.21 %), and geranyl acetate (15.64-12.79 %) were the main constituents of the oil which were obtained in 0,32-0.39 % yield. The biological activity of this oil was reported.


2018 ◽  
Vol 16 (S1) ◽  
pp. S48-S54
Author(s):  
Y. Ez zoubi ◽  
S. Lairini ◽  
A. Farah ◽  
K. Taghzouti ◽  
A. El Ouali Lalami

The purpose of this study was to determine the chemical composition and to evaluate the antioxidant and antibacterial effects of the Moroccan Artemisia herba-alba Asso essential oil against foodborne pathogens. The essential oil of Artemisia herba-alba was analyzed by gas chromatography coupled with mass spectroscopy. The antibacterial activity was assessed against three bacterial strains isolated from foodstuff and three bacterial strains referenced by the ATCC (American Type Culture Collection) using the disk diffusion assay and the macrodilution method. The antioxidant activity was evaluated using the DPPH (2, 2-diphenyl-1- picrylhydrazyl) method. The fourteen compounds of the Artemisia herba-alba essential oil were identified; the main components were identified as β-thujone, chrysanthenone, α-terpineol, α-thujone, α-pinene, and bornyl acetate. The results of the antibacterial activity obtained showed a sensitivity of the different strains to Artemisia herba-alba essential oil with an inhibition diameter of 8.50 to 17.00 mm. Concerning the MICs (minimum inhibitory concentrations), the essential oil exhibited much higher antibacterial activity with MIC values of 2.5 μl/ml against Bacillus subtilis ATCC and Lactobacillus sp. The essential oil was found to be active by inhibiting free radicals with an IC50 (concentration of an inhibitor where the response is reduced by half) value of 2.9 μg/ml. These results indicate the possible use of the essential oil on food systems as an effective inhibitor of foodborne pathogens, as a natural antioxidant, and for potential pharmaceutical applications. However, further research is needed in order to determine the toxicity, antibacterial, and antioxidant effects in edible products.


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