Chemical Characterization and Antibacterial Activity of the Essential Oil of Coriandrum sativum Leaves in the West of Iran (Kermanshah)

Background: Fruit peels are considered as waste and contribute towards a major proportion of biomass. They might be a good source of various therapeutic benefits. Peels biomass of citrus fruits are usually considered as garbage. Such peels may have many important and valuable medicinal components with pharmacological activities. Citrus reticulata, (of family Rutaceae, local name tangerine) is a local seasonal fruit in Pakistan which is a very good example regarding wastage of its peels. Objective: The study is based on exploration of a citrus fruit peel derived essential oil, its chemical characterization, identification of various bioactive components and the exploration of pharmacological potentials (antibacterial and wound healing activity). Method: Essential oil was recovered by hydro-distillation of freshly collected peels. Chemical constituents of oil were determined by gas chromatography-mass spectroscopy (GC-MS) analysis. Antioxidant activities were evaluated by total phenolic contents, total flavonoid content, DPPH scavenging activity and reducing power assay. Antibacterial activity was determined using disc diffusion assay. In vivo wound healing potential was determined in rabbits after topical administration of oil. Wound scoring was calculated followed by histological study. Results: GC-MS analysis showed presence of various components with greatest proportion of D-Limonene (89.31%). Total flavonoid and phenolic contents were found to be 14.63 ± 0.95 mg CE/g and 17.03 ± 3.24 mg GAE/g respectively while DPPH activity was found to be 73.32%. Better antibacterial activity was shown against E. coli. In vivo studies showed significant reduction in wound diameter in essential oil treatment groups. Further the essential oil was found non-irritant in draize scoring. Conclusion: The study concluded that essential oil of this fruit peel might be used for antibacterial and wound healing purposes.

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Brocchia cinerea essential oil from Brezina (Algerian Sahara): Chemical characterization and antibacterial activity

10.47890/jcsce/2020/acheriti/10082113 ◽

2020 ◽

Vol 1 (1) ◽

Author(s):

Cheriti A ◽

Boukhobza Z ◽

Boulenouar N ◽

Djeradi H ◽

M Kacimi El Hassani ◽

...

Keyword(s):

Antibacterial Activity ◽

Essential Oil ◽

Chemical Characterization ◽

Klebsiella Pneumonia ◽

Gram Positive Bacteria ◽

Oxygenated Monoterpenes ◽

Algerian Sahara ◽

Total Oil ◽

Diffusion Assay ◽

And Staphylococcus Aureus

The essential oil of wild-growing medicinal specie Brocchia cinerea (Asteraceae) from Brezina (Algerian Sahara) was studied for chemical composition by GC/MS analysis and antibacterial activity. Twenty-nine identified components constituted 94.07% of the oil. The most important major components in the oil were trans-Thujone (36.11 %), Camphor (12.08 %), Santolina triene (11.25 %), 1,8-Cineol (6.98 %) and Cis-Verbenyl acetate (6.33 %), together reaching 72.75% of the total oil which is characterized by the presence of high content of oxygenated monoterpenes. The antibacterial activity of the essential oil was tested using the disc diffusion assay. The results showed that the essential oil of Brocchia cinerea had antibacterial activity against all tested Gram-positive bacteria (Enterococcus faecalis and Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli, Klebsiella pneumonia, and Pseudomonas aereginosa). Keywords: Essential Oil; Brocchia Cinerea; GC/MS; Oxygenated Monoterpenes; Antibacterial activity; Sahara;

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Physico-chemical characterization and antibacterial activity of inclusion complexes of Hyptis martiusii Benth essential oil in β-cyclodextrin

Biomedicine & Pharmacotherapy ◽

10.1016/j.biopha.2017.01.158 ◽

2017 ◽

Vol 89 ◽

pp. 201-207 ◽

Cited By ~ 26

Author(s):

Tatianny A. Andrade ◽

Thiago S. Freitas ◽

Francielly O. Araújo ◽

Paula P. Menezes ◽

Grace Anne A. Dória ◽

...

Keyword(s):

Antibacterial Activity ◽

Essential Oil ◽

Inclusion Complexes ◽

Chemical Characterization ◽

Physico Chemical

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Microencapsulation, chemical characterization, and antibacterial activity of Citrus hystrix DC (Kaffir Lime) peel essential oil

Monatshefte für Chemie - Chemical Monthly ◽

10.1007/s00706-017-2015-8 ◽

2017 ◽

Vol 148 (7) ◽

pp. 1229-1234 ◽

Cited By ~ 7

Author(s):

Apinya Raksa ◽

Panida Sawaddee ◽

Phathaitep Raksa ◽

Arunee Kongdee Aldred

Keyword(s):

Antibacterial Activity ◽

Essential Oil ◽

Chemical Characterization ◽

Lime Peel

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Chemical characterization and antimicrobial activity of Campomanesia aurea against three strains of Listeria monocytogenes

Brazilian Journal of Biology ◽

10.1590/1519-6984.219889 ◽

2020 ◽

Author(s):

L. A. Pacheco ◽

E. M. Ethur ◽

T. Sheibel ◽

B. Buhl ◽

A. C. Weber ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Listeria Monocytogenes ◽

Essential Oil ◽

Essential Oils ◽

Chemical Characterization ◽

Culture Collection ◽

American Type Culture Collection ◽

Antibiofilm Activity ◽

Bacteriostatic Effect

Abstract The essential oil (EO) composition of leaves of Campomanesia aurea was analyzed by GC-MS. The plant was collected in April (EOA) and October (EOO) in São Francisco de Assis/RS. The essential oils were obtained by hydrodistillation in a modified Clevenger apparatus. The yields were 4.44% in April, and 6.15%, in October. Thirty-one compounds were identified in EOA, accounting for 96.87% of the total, and twenty-eight compounds were identified in EOO, accounting for 99.46% of the total. For EOA, the major identified monoterpene was p-cymene (8.33%) and the major identified sesquiterpene was α-cadinol (10.72%), while regarding EOO the major identified monoterpene was repeatedly Terpinolene (3.43%), whereas the major identified sesquiterpene was α-cadinol (12.79%). The antibacterial activity of the essential oil was evaluated against the three Listeria monocytogenes from the American Type Culture Collection (strains ATCC 7644, 19114 and 13932). The essential oil showed a MIC of 10 mg/mL against the strains ATCC 7644 and 19114, with bacteriostatic effect of 20 mg/mL and a MIC of 20 mg/mL for ATCC 13932. Regarding the antibiofilm activity, o EOO showed the best results with an inhibition of 90% in the sessile growth with an applied concentration of 4.0 mg/mL.

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TLC-Direct Bioautography for Determination of Antibacterial Activity of Artemisia adamsii Essential Oil

Journal of AOAC International ◽

10.5740/jaoacint.sgehorvath ◽

2013 ◽

Vol 96 (6) ◽

pp. 1209-1213 ◽

Cited By ~ 11

Author(s):

Györgyi Horváth ◽

Kamilla Ács ◽

Béla Kocsis

Keyword(s):

Antibacterial Activity ◽

Essential Oil ◽

Pathogenic Bacteria ◽

Agar Plate ◽

Chemical Characterization ◽

Medicinal Value ◽

Microbiological Methods ◽

Artemisia Adamsii ◽

Direct Bioautography ◽

Main Component

Abstract The aim of the present study was the chemical characterization of the essential oil of a Mongolian medicinal plant, Artemisia adamsii Besser, and the investigation of the antibacterial effect of its oil on different human pathogenic bacteria (Staphylococcus aureus, methicillin-resistant S. aureus, and S. epidermidis). The chemical composition of the oil was established by GC and GC/MS. Direct bioautography was used for detecting the antibacterial activity of the essential oil. The result of GC experiments showed that α-thujone was the main component (64.4%) of the oil, while the amount of β-thujone was 7.1%. 1,8-Cineole seemed to be the other relevant component (15.2%). The antibacterial activity of the A. adamsii essential oil against all three investigated bacteria was observed in the bioautographic system, but this effect was not proportional to the concentrations of α- or β-thujone; therefore, from a microbiological aspect, thujone content does not determine the medicinal value of this oil. On the whole, the combination of TLC separation with biological detection is an appropriate method for evaluating multicomponent and hydrophobic plant extracts, for instance, essential oils, and it provides more reliable results than traditional microbiological methods (e. g., disc diffusion and agar plate techniques).

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Chemical Composition and In Vitro Antibacterial Activity of the Essential Oil of Phthirusa adunca from Venezuelan Andes

Natural Product Communications ◽

10.1177/1934578x1100600731 ◽

2011 ◽

Vol 6 (7) ◽

pp. 1934578X1100600 ◽

Cited By ~ 1

Author(s):

Flor D. Mora ◽

Nurby Ríos ◽

Luis B. Rojas ◽

Tulia Díaz ◽

Judith Velasco ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Essential Oil ◽

Chemical Characterization ◽

Salmonella Typhi ◽

Germacrene D ◽

In Vitro Antibacterial Activity

In this paper, preliminary studies on the chemical characterization of Phthirusa adunca Meyer essential oil, obtained by hydrodistillation, is presented. The separation of the components was performed by GC-MS. Twenty-three compounds (94.5% of the sample) were identified of which the three major ones (76% of the sample) were β-phellandrene (38.1%), germacrene D (26.8%) and β-pinene (11.5%). The essential oil showed a broad spectrum of activity against Salmonella Typhi CDC 57 (100 μg/mL), Staphylococcus aureus ATCC 25923 (200 μg/mL), Enterococcus faecalis ATCC 29212 (250 μg/mL), Escherichia coli ATCC 25922 y Klebsiella pneumoniae ATCC 23357 (500 μg/mL). This is the first report on the composition and activity of the essential oil of this species.

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Chemical Profiling, Antioxidant, and Antimicrobial Activity against Drug-Resistant Microbes of Essential Oil from Withania frutescens L.

Applied Sciences ◽

10.3390/app11115168 ◽

2021 ◽

Vol 11 (11) ◽

pp. 5168

Author(s):

Abdelfattah EL Moussaoui ◽

Mohammed Bourhia ◽

Fatima Zahra Jawhari ◽

Ahmad Mohammad Salamatullah ◽

Riaz Ullah ◽

...

Keyword(s):

Antibacterial Activity ◽

Essential Oil ◽

Antioxidant Activities ◽

Chemical Characterization ◽

Multidrug Resistant ◽

Gas Chromatography Mass Spectrometry ◽

Resistant Bacteria ◽

Free Radical Damage ◽

Antibacterial And Antifungal Activities ◽

Β Carotene

This work was conducted to study the chemical composition, antioxidant, antibacterial, and antifungal activities of essential oil and hydrolat from Withania frutescens. The essential oil was extracted by hydrodistillation. The chemical characterization was performed using gas chromatography-mass spectrometry (GC/MS). The antioxidant activity was studied using four different assays (DPPH, TAC, FRAP, and β-carotene bleaching). The antibacterial activity test was carried out on multidrug-resistant bacteria including Gram-negative and Gram-positive strains. Antifungal activity was tested on Candida albicans and Saccharomyces cerevisiae. The yield of essential oil (EO) obtained by hydrodistillation of W. frutescens was 0.31% majorly composed of camphor, α-thujone, carvacrol, and thymol. Regarding the antioxidant activities, the concentration of the sample required to inhibit 50% of radicals (IC50) of EO and hydrolat were 14.031 ± 0.012 and 232.081 ± 3.047 µg/mL (DPPH), 4.618 ± 0.045 and 8.997 ± 0.147 µg/mL (FRAP), 0.091 ± 0.007 and 0.131 ± 0.004 mg AAE/mg (TAC), 74.141 ± 1.040% and 40.850 ± 0.083% (β-carotene), respectively. Concerning the antibacterial activity of essential oil and hydrolat, the minimum inhibitory concentration (MIC) values found were 0.006 ± 0.001 and 6.125 ± 0.541 µg/mL (Escherichia coli 57), 0.003 ± 0.001 and 6.125 ± 0.068 µg/mL (Klebsiella pneumoniae), 0.001 ± 0.0 and 6.125 ± 0.046 µg/mL (Pseudomonas aeruginosa) and 0.012 ± 0.003 and 6.125 ± 0.571 µg/mL (Staphylococcus aureus), respectively. MIC values of essential oil and hydrolat vs. both C. albicans and S. cerevisiae were lower than 1/20,480 µg/mL. Based on the findings obtained, essential oils of Withania frutescens can be used as promising natural agents to fight free radical damage and nosocomial antibiotic-resistant microbes.

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