scholarly journals Evaluation of the Most Current and Effective Methods in the Analysis of Chlorinated Dioxins in Ground Beef

2003 ◽  
Vol 3 ◽  
pp. 913-921 ◽  
Author(s):  
Ebere C. Anyanwu ◽  
Mohamed H. El-Saeid ◽  
Akpan I. Akpan ◽  
Mahmoud A. Saled
Keyword(s):  
Food Chain ◽  
Environmental Pollutants ◽  
Ground Beef ◽  
Extraction Methods ◽  
Sample Introduction ◽  
Animal Origin ◽  
Clear Understanding ◽  
Daily Consumption ◽  
Contaminated Food ◽  
Chlorinated Dioxins

Chlorinated dioxins are the group of environmental pollutants consisting of 210 chlorinated dibenzo-p-dioxins and dibenzofurans. They are highly toxic and persistent. They are lipophilic and can easily biomagnify in the food chain, hence posing a serious threat to human health. The daily consumption of low-level contaminated food, mainly of animal origin, leads to the accumulation of dioxins in the human body. The exposures of the general human population to dioxins and the specific issues of a risk assessment of dioxin pose serious concerns in public environmental and nutritional health. This paper reviews the analysis of chlorinated dioxins in ground beef. The sources of contamination of chlorinated dioxins in ground beef are first reviewed to form a basis for a clear understanding of the health implications of chlorinated dioxins in the human food chain and why it is necessary to monitor the level of dioxins in animal food products, especially ground beef. The methods of collection, sampling, and processing of ground beef, and the methods of sample clean up prior to the analysis, are reviewed. Emphasis is laid on the new techniques that are available and that might be effective in the analysis of chlorinated dioxins in ground beef. Among these new methods and techniques are: the synergistic combination of ELISA/GC/MS, direct sample introduction to /GC/MS-MS, automated clean-up method, and the supercritical fluid extraction methods. The possible treatments of results from each method and technique are discussed and their respective efficiencies are compared. Finally, quality control and quality assurance parameters are evaluated for levels of accuracy, reproducibility, and precision.

scholarly journals Natural Energy Bars With Protein Improvement From Animal Origin Foods

2021 ◽  
Author(s):  
M. A. Logroño Veloz ◽  
S. L. Betancourt Ortiz ◽  
J. G. Fonseca
Keyword(s):  
Protein Quality ◽  
Low Cost ◽  
Egg White ◽  
Animal Origin ◽  
Daily Consumption ◽  
Powdered Milk ◽  
Natural Energy ◽  
Product Acceptability ◽  
Technological Quality ◽  
Cereal Bars

The energy bars provide the consumer with nutritional and organoleptic quality, as well as a prolonged shelf life without the need to modify the temperature for storage, however, they do not satisfy the nutritional requirements of high-quality proteins since they are usually made from cereals, which are low cost ingredients and great energy contribution. The objective of this study was to create an energetic and nutritious bar, without diminishing the technological quality of the commercial bars and that, due to their sensory characteristics, are easily included in the daily consumption of people who exercise and want to control their weight. A product was formulated with a greater contribution and protein quality, and with a functional dose of macronutrients. The product was prepared in compliance with the regulations of the Ecuadorian Institute for Standardization (INEN) and the Food Codex, the premixing and roasting of ingredients was controlled and finally food with animal protein source such as egg white and powdered milk was added. For the premix oat flakes, nuts such as nuts, almonds and hazelnuts, chia seeds and honey were used, then added egg white and powdered milk. The protein content it reached was 29.01% on a wet basis, a fat value of 23.10% and carbohydrates of 25.24% on 100 g of sample on a wet basis. The energy distribution of macronutrients was balanced, and the sensory evaluation showed good product acceptability. Keywords: cereal bars, protein, immediate energy. Resumen Las barras energéticas proporcionan al consumidor calidad nutricional y organoléptica, así como una vida de anaquel prolongada sin necesidad de modificar la temperatura para su almacenamiento, sin embargo, no satisfacen los requerimientos nutricionales de proteínas de alta calidad ya que generalmente están elaboradas a base de cereales, que son ingredientes de bajo costo y gran aporte energético. El objetivo se este estudio fue crear una barra energética y nutritiva, sin disminuir la calidad tecnológica de las barras comerciales y que por sus características sensoriales sean fácilmente incluidas en el consumo diario de personas que se ejercitan y quieren controlar su peso. Se formuló un producto con un mayor aporte y calidad proteica, y con una dosis funcional de macronutrientes. El producto se elaboró cumpliendo las normativas del Instituto Ecuatoriano de Normalización (INEN) y del Codex alimentario, se controló la premezcla y tostado de ingredientes y finalmente se adicionó los alimentos con fuente proteica animal como la clara de huevo y leche en polvo. Para la premezcla se utilizaron hojuelas de avena, frutos secos como nueces, almendras y avellanas, semillas de chía y miel de abeja, luego se adicionó la clara de huevo y la leche en polvo. El contenido proteico que alcanzó fue de 29,01% en base húmeda, un valor en grasas de 23,10% y de carbohidratos de 25,24% de muestra en base húmeda. La distribución energética de macronutrientes fue equilibrada y la evaluación sensorial arrojó una buena aceptabilidad del producto. Palabras claves: barras de cereales, proteína, energía inmediata.

scholarly journals Incomplete sanitation of a meat grinder and ingestion of raw ground beef: contributing factors to a large outbreak of Salmonella Typhimurium infection

1997 ◽  
Vol 119 (2) ◽  
pp. 127-134 ◽  
Author(s):  
T. H. ROELS ◽  
P. A. FRAZAK ◽  
J. J. KAZMIERCZAK ◽  
W. R. MACKENZIE ◽  
M. E. PROCTOR ◽  
...  
Keyword(s):  
Ground Beef ◽  
The United States ◽  
Case Control ◽  
Animal Origin ◽  
Health Warnings ◽  
Contributing Factors ◽  
Case Control Studies ◽  
Large Outbreak ◽  
Butcher Shop ◽  
Control Study

Consumers in the United States continue to eat raw or undercooked foods of animal origin despite public health warnings following several well-publicized outbreaks. We investigated an outbreak of Salmonella serotype Typhimurium infection in 158 patients in Wisconsin during the 1994 Christmas holiday period. To determine the vehicle and source of the outbreak, we conducted cohort and case-control studies, and environmental investigations in butcher shop A. Eating raw ground beef purchased from butcher shop A was the only item significantly associated with illness [cohort study: relative risk=5·8, 95% confidence interval (CI)=1·5–21·8; case control study: odds ratio=46·2, 95% CI=3·8–2751]. Inadequate cleaning and sanitization of the meat grinder in butcher shop A likely resulted in sustained contamination of ground beef during an 8-day interval. Consumer education, coupled with hazard reduction efforts at multiple stages in the food processing chain, will continue to play an important role in the control of foodborne illness.

A computer model of cadmium metabolism in the sheep with regard to the human food chain

1999 ◽  
Vol 1999 ◽  
pp. 15-15
Author(s):  
S.H. Prankel
Keyword(s):  
Sewage Sludge ◽  
Food Chain ◽  
Animal Feed ◽  
Animal Origin ◽  
Kidney Cortex ◽  
Human Consumption ◽  
Toxic Heavy Metal ◽  
Animal Products ◽  
Human Food ◽  
Human Food Chain

Cadmium (Cd) is a non-essential toxic heavy metal which is added to soil in phosphorous fertilizers and sewage sludge. Cd enters the human food chain via animals fed on crops from these soils, food of vegetable origin and smoking. In chronic exposure situations Cd accumulates in the liver and kidney of animals and man. Intake resulting in a Cd concentration of over 200μg/g wet weight in the kidney cortex results in kidney damage in humans (Friberg et al., 1974). With the prohibition of disposal of sewage sludge at sea in 1998, it is likely that more Cd will be deposited on soils. Current limits for Cd in animal feed are intended to prevent food from animal origin from exceeding legal Cd limits. This work examines the behaviour of Cd in the ovine body and models organ Cd from given intakes to investigate whether current limits in feed are safe and result in animal products fit for human consumption.

scholarly journals A Proof of Principle for the Detection of Viable Brucella spp. in Raw Milk by qPCR Targeting Bacteriophages

2020 ◽  
Vol 8 (9) ◽  
pp. 1326
Author(s):  
Michaela Projahn ◽  
Jens A. Hammerl ◽  
Ralf Dieckmann ◽  
Sascha Al Dahouk
Keyword(s):  
Limit Of Detection ◽  
Raw Milk ◽  
Qpcr Assay ◽  
Animal Origin ◽  
Indicator Organisms ◽  
Food Of Animal Origin ◽  
Global Health Issue ◽  
Contaminated Food ◽  
Viable Bacteria ◽  
And Performance

Brucellosis is still a global health issue, and surveillance and control of this zoonotic disease in livestock remains a challenge. Human outbreaks are mainly linked to the consumption of unpasteurized dairy products. The detection of human pathogenic Brucella species in food of animal origin is time-consuming and laborious. Bacteriophages are broadly applied to the typing of Brucella isolates from pure culture. Since phages intracellularly replicate to very high numbers, they can also be used as specific indicator organisms of their host bacteria. We developed a novel real-time PCR (qPCR) assay targeting the highly conserved helicase sequence harbored in all currently known Brucella-specific lytic phages. Quality and performance tests determined a limit of detection of <1 genomic copy/µL. In raw milk artificially contaminated with Brucella microti, Izv phages were reliably detected after 39 h of incubation, indicating the presence of viable bacteria. The qPCR assay showed high stability in the milk matrix and significantly shortened the time to diagnosis when compared to traditional culture-based techniques. Hence, our molecular assay is a reliable and sensitive method to analyze phage titers, may help to reduce the hands-on time needed for the screening of potentially contaminated food, and reveals infection risks without bacterial isolation.

scholarly journals Quantitative LAMP and PCR Detection of Salmonella in Chicken Samples Collected from Local Markets around Pathum Thani Province, Thailand

2020 ◽  
Vol 2020 ◽  
pp. 1-6 ◽  
Author(s):  
Virun Vichaibun ◽  
Panan Kanchanaphum
Keyword(s):  
Efficient Method ◽  
Food Chain ◽  
Dna Detection ◽  
Pcr Detection ◽  
Isothermal Amplification ◽  
Artificial Infection ◽  
Conventional Pcr ◽  
Loop Mediated Isothermal Amplification ◽  
Contaminated Food ◽  
Pcr Method

Salmonella is a bacterium that infects people when they consume contaminated food or liquids. To prevent humans from becoming ill, it is useful to have an efficient method of detecting Salmonella before the disease is passed on through the food chain. In this research, the efficiency of Salmonella detection was compared using the following four methods: conventional loop-mediated isothermal amplification (LAMP), PCR, quantitative LAMP (qLAMP), and qPCR. The artificial infection of chicken samples started with incubating of 10 mL of 108 CFU of S. typhimurium for 6 hr. and enriching for 2 hr. to represent real contamination of the samples. The results show that the sensitivity of Salmonella DNA detection in PCR, qPCR, LAMP, and qLAMP were 50 ng, 5 ng, 50 pg, and and 500 fg, respectively. Thirty samples of 10 g chicken were collected from 10 markets in Pathum Thani, Thailand; then, the infection was detected. The conventional LAMP, qLAMP, and qPCR methods detected Salmonella in all the chicken samples. However, the conventional PCR method detected Salmonella infection in only eight of the samples. Overall, the qLAMP method had the highest sensitivity of Salmonella DNA detection.

Environmental pollutants and marine mammal health: The potential impact of hydrocarbons and halogenated hydrocarbons on immune system dysfunction

2020 ◽  
pp. 223-248
Author(s):  
David Busbee ◽  
Ian Tizard ◽  
Jeffrey Sroit ◽  
Davide Ferrirc ◽  
Ellen Orr-reeves
Keyword(s):  
Immune System ◽  
Food Chain ◽  
Marine Mammal ◽  
Marine Mammals ◽  
Large Scale ◽  
Environmental Pollutants ◽  
Halogenated Hydrocarbons ◽  
Mechanisms Of Toxicity ◽  
Terrestrial Mammals ◽  
Immune System Dysfunction

This paper provides a detailed review of the immunotoxicological effects of environmental pollutants on the health of marine mammals, particularly in relation to their impact on the immune system and mechanisms of toxicity. Environmental pollutants are increasingly implicated (both directly and indirectly) with the onset of infectious disease and related mortality incidents in marine mammals,. The release of chemicals into the marine environment and the subsequent bioaccumulation up the food chain may pose a serious threat to marine mammals inhabiting contaminated areas; this has been documented in various studies of pollutant concentrations in tissue samples and large scale mass mortalities. Data correlating pollutant residues with altered reproductive/developmental states, and immune system dysfunction in particular, are reported for terrestrial mammals and suggest a similar association in marine mammals. Immunology is emphasised as a tool for assessing marine mammal health using quantitative and qualitative techniques to establish the effects of chemical pollutants. This has become increasingly important in relation to the subsequent dangers that may be posed to humans through any indirect exposure via the food chain.

Accumulation of Polychlorinated Biphenyls in Mussels: A Proteomic Study

2018 ◽  
Vol 81 (2) ◽  
pp. 316-324 ◽  
Author(s):  
Letizia Ambrosio ◽  
Rosario Russo ◽  
Anna Maria Salzano ◽  
Francesco Paolo Serpe ◽  
Andrea Ariano ◽  
...  
Keyword(s):  
Polychlorinated Biphenyls ◽  
Protein Expression ◽  
Environmental Pollutants ◽  
Specific Protein ◽  
Animal Origin ◽  
Proteomic Approach ◽  
Food Of Animal Origin ◽  
Seafood Products ◽  
Living Organisms ◽  
Analytical Approaches

ABSTRACT Polychlorinated biphenyls (PCBs) are environmental pollutants of industrial origin that can contaminate food, mainly food of animal origin. Although production of PCBs has been banned in many countries since the 1980s, they are still present in the environment and are considered dangerous pollutants for human health. In fact, they can bioaccumulate in living organisms such as marine organisms because of their chemical and physical properties. New analytical approaches are useful to monitor the presence of such contaminants in seafood products and in the environment. In this work, we evaluate changes in protein expression of Mytilus galloprovincialis (Lam.) experimentally exposed to a PCB mixture and identify chemically specific protein expression signatures by using a proteomic approach. In particular, we identify 21 proteins whose levels of expression are sensibly modified after 3 weeks of exposure. The present work shows that a proteomic approach can be a useful tool to study alterations of protein expression in mussels exposed to PCBs and represents a first step toward the development of screening protocols to be used for biomonitoring surveys of fishery products.

scholarly journals Extraction methods and test techniques for detection of vegetable proteins in meat products:I. Qualitative detection of soya derivatives

1976 ◽  
Vol 76 (3) ◽  
pp. 329-336 ◽  
Author(s):  
N. ST G. Hyslop
Keyword(s):  
Sodium Dodecyl Sulphate ◽  
Vegetable Protein ◽  
Sodium Dodecyl ◽  
Meat Products ◽  
Extraction Methods ◽  
Soya Bean ◽  
Animal Origin ◽  
Polyacrylamide Gels ◽  
Migration Rates ◽  
Qualitative Detection

SUMMARYExtracts of 3 soya bean preparations, used commercially in certain countries to replace part of the meat in popular meat products, were made by treatment with (i) sodium dodecyl sulphate, (ii) Triton-X100 or (iii) n–Butanol. Similar extracts were made from beef and pork.All extracts were examined by electrophoretic and immunological techniques. Stained polyacrylamide gels revealed distinctive protein bands after electrophoresis. The migration rates of corresponding bands differed between beef and pork extracts. However, the migration rates of vegetable bands revealed certain similarities, but differed very greatly from those of animal origin. Characteristic fast-migrating S-bands were distinguishable only in extracts of vegetable protein. Immunodiffusion tests, using antisera produced in rabbits against each extract, revealed varying degrees of similarity between extracts of vegetable origin, but the antisera were specific for either vegetable or animal protein.

scholarly journals A Brief Survey for MicroRNA Precursor Identification Using Machine Learning Methods

2020 ◽  
Vol 21 (1) ◽  
pp. 11-25 ◽  
Author(s):  
Zheng-Xing Guan ◽  
Shi-Hao Li ◽  
Zi-Mei Zhang ◽  
Dan Zhang ◽  
Hui Yang ◽  
...  
Keyword(s):  
Machine Learning ◽  
Comparative Genomics ◽  
Experimental Methods ◽  
Extraction Methods ◽  
Clear Understanding ◽  
Learning Methods ◽  
Accurate Identification ◽  
Rna Molecules ◽  
Machine Learning Methods ◽  
Benchmark Datasets

MicroRNAs, a group of short non-coding RNA molecules, could regulate gene expression. Many diseases are associated with abnormal expression of miRNAs. Therefore, accurate identification of miRNA precursors is necessary. In the past 10 years, experimental methods, comparative genomics methods, and artificial intelligence methods have been used to identify pre-miRNAs. However, experimental methods and comparative genomics methods have their disadvantages, such as timeconsuming. In contrast, machine learning-based method is a better choice. Therefore, the review summarizes the current advances in pre-miRNA recognition based on computational methods, including the construction of benchmark datasets, feature extraction methods, prediction algorithms, and the results of the models. And we also provide valid information about the predictors currently available. Finally, we give the future perspectives on the identification of pre-miRNAs. The review provides scholars with a whole background of pre-miRNA identification by using machine learning methods, which can help researchers have a clear understanding of progress of the research in this field.

scholarly journals Methods to Detect Viruses in Foods: Testing and Interpretation of Results1

1983 ◽  
Vol 46 (4) ◽  
pp. 345-357 ◽  
Author(s):  
D. O. CLIVER ◽  
R. D. ELLENDER ◽  
M. D. SOBSEY
Keyword(s):  
Cell Cultures ◽  
Ground Beef ◽  
Raw Milk ◽  
Extraction Methods ◽  
Food Samples ◽  
Foodborne Disease ◽  
Bivalve Molluscs ◽  
Large Numbers ◽  
Commercial Species

Viruses that may be detected in foods should be considered pathogenic and treated with appropriate caution. In this discussion, specific procedures for extracting viruses from shellfish are presented for each of the major commercial species of bivalve molluscs. Other foods for which specific extraction methods are detailed include lettuce, frozen strawberries, ground beef and raw milk. Viruses that may be detected by the methods described are those which are capable of producing a perceptible effect while replicating in cultured primate cells. Both results that are apparently positive and those that are apparently negative require careful interpretation; one must be extremely skeptical if large numbers of food samples obtained at the market appear to yield viruses. The procedures that are now available have some important limitations, including inability to detect the viruses that cause most of the reported foodborne disease. Approaches to surmounting these limitations include use of serologic methods to detect viruses that do not cause perceptible effects in cell cultures and improvement of procedures for extracting all viruses from food samples.

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