scholarly journals SLALOM: A Simple and Rapid Method for Enzymatic Synthesis of CRISPR-Cas9 sgRNA Libraries

2020 ◽  
Author(s):  
Joshua D Yates ◽  
Robert C Russell ◽  
H Joseph Yost ◽  
Jonathon T Hill
Keyword(s):  
New Technologies ◽  
Technical Difficulty ◽  
Solid Supports ◽  
Vast Number ◽  
Chromatin Dynamics ◽  
E Coli ◽  
Time Requirements ◽  
Novel Method ◽  
The Cost ◽  
Robust Variant

ABSTRACTCRISPR-Cas9 sgRNA libraries have transformed functional genetic screening and have enabled innovative CRISPR-based methods, such as the visualization of chromatin dynamics in living cells. These libraries have the potential to be applied to a vast number of biological systems and aid in the development of new technologies, but their synthesis is hindered by the cost, time requirements, and technical difficulty of current sgRNA library generation methods. Here, we describe SLALOM—a rapid enzymatic method for generating robust, variant-matched sgRNA libraries from any source of DNA in under 3 hours. This method utilizes a custom sgRNA scaffold sequence and a novel method for detaching oligonucleotides from solid supports using a strand displacing polymerase. Using this method, we have constructed libraries targeting the E. coli genome and the transcriptome of developing zebrafish hearts, demonstrating its potential to expand the reach of CRISPR technology and facilitate methods requiring custom sgRNA libraries.

Highly Active Modified Variants of Recombinant Phospholipase А2 from Streptomyces violaceoruber for Effective Biosynthesis in Yeasts

2019 ◽  
pp. 30-41 ◽  
Author(s):  
E.P. Sannikova ◽  
A.V. Malysheva ◽  
F.A. Klebanov ◽  
D.G. Kozlov
Keyword(s):  
New Technologies ◽  
Specific Activity ◽  
Point Mutations ◽  
Egg Yolk ◽  
Enzyme Preparations ◽  
Highly Active ◽  
High Calcium ◽  
Pla2 Enzyme ◽  
Streptomyces Violaceoruber ◽  
The Cost

The capacity of yeast to produce the highly active variants of PLA2 has been confirmed. The high-active variants were based on the original enzyme from the strain А-2688 of Streptomyces violaceoruber. To reduce the enzyme toxicity and to increase its expression, various approaches were tested including point mutations, construction of artificial N- and/or C-end pro-regions, hybridization with other proteins and engineering or inactivation of glycosylation sites. As a main result, the modified PLA2 enzymes were obtained which have the same secretion level as their low-active predecessors, but specific activity of which was at least tenfold higher. As the main feature, the selected mutants were characterized by a lower affinity for Ca2+ that probably accounts for their low toxicity (and high expression capacity) at the stage of biosynthesis and their ability to activate under special conditions, e.g. during the egg yolk fermentation. The data obtained can provide a basis for the cost reduction of highly active PLA2 enzyme preparations in industries where the application of high calcium concentrations is allowed. recombinant phospholipase А2, Streptomyces violaceoruber, yeasts, secretion, producer strain The work was initiated by the Innovation Center Biriuch - New Technologies, Ltd., and was supported within the framework of the State Assignment no. 595-00004-18 PR.

scholarly journals Design and Adoption of Low-Cost Point-of-Care Diagnostic Devices: Syrian Case

Micromachines ◽  
2021 ◽  
Vol 12 (8) ◽  
pp. 882
Author(s):  
M. Munzer Alseed ◽  
Hamzah Syed ◽  
Mehmet Cengiz Onbasli ◽  
Ali K. Yetisen ◽  
Savas Tasoglu
Keyword(s):  
Civil Wars ◽  
Healthcare Workers ◽  
New Technologies ◽  
Point Of Care ◽  
Low Cost ◽  
Healthcare Services ◽  
Efficient Solutions ◽  
Humanitarian Crises ◽  
Technology Experience ◽  
The Cost

Civil wars produce immense humanitarian crises, causing millions of individuals to seek refuge in other countries. The rate of disease prevalence has inclined among the refugees, increasing the cost of healthcare. Complex medical conditions and high numbers of patients at healthcare centers overwhelm the healthcare system and delay diagnosis and treatment. Point-of-care (PoC) testing can provide efficient solutions to high equipment cost, late diagnosis, and low accessibility of healthcare services. However, the development of PoC devices in developing countries is challenged by several barriers. Such PoC devices may not be adopted due to prejudices about new technologies and the need for special training to use some of these devices. Here, we investigated the concerns of end users regarding PoC devices by surveying healthcare workers and doctors. The tendency to adopt PoC device changes is based on demographic factors such as work sector, education, and technology experience. The most apparent concern about PoC devices was issues regarding low accuracy, according to the surveyed clinicians.

scholarly journals Embracing Industry 4.0: Empirical Insights from Malaysia

Informatics ◽  
2021 ◽  
Vol 8 (2) ◽  
pp. 30
Author(s):  
Mansoor Ahmed Soomro ◽  
Mohd Hizam-Hanafiah ◽  
Nor Liza Abdullah ◽  
Mohd Helmi Ali ◽  
Muhammad Shahar Jusoh
Keyword(s):  
Industry 4.0 ◽  
New Technologies ◽  
Descriptive Study ◽  
Digital Transformation ◽  
Quantitative Approach ◽  
Survey Questionnaire ◽  
Digital Revolution ◽  
Leadership Strategy ◽  
The Right ◽  
The Cost

Industry 4.0 revolution, with its cutting-edge technologies, is an enabler for businesses, particularly in reducing the cost and improving the productivity. However, a large number of organizations are still too in their infancy to leverage the true potential of Industry 4.0 and its technologies. This paper takes a quantitative approach to reveal key insights from the companies that have implemented Industry 4.0 technologies. For this purpose, 238 technology companies in Malaysia were studied through a survey questionnaire. As technology companies are usually the first in line to adopt new technologies, they can be studied better as leaders in adopting the latest technologies. The findings of this descriptive study surfaced an array of insights in terms of Industry 4.0 readiness, Industry 4.0 technologies, leadership, strategy, and innovation. This research paper contributes by providing 10 key empirical insights on Industry 4.0 that can be utilized by managers to pace up their efforts towards digital transformation, and can help the policymakers in drafting the right policy to drive the digital revolution.

scholarly journals A Novel Method for the Complex Tube System Reconstruction and Measurement

Sensors ◽  
2021 ◽  
Vol 21 (6) ◽  
pp. 2207
Author(s):  
Xiang Guo ◽  
Xin Su ◽  
Yingtao Yuan ◽  
Tao Suo ◽  
Yan Liu
Keyword(s):  
Neural Network ◽  
Minimum Distance ◽  
Technical Difficulty ◽  
Industry Structure ◽  
Tube System ◽  
Simulation Experiments ◽  
Pipe System ◽  
System Reconstruction ◽  
Novel Method ◽  
Assembly Error

Pipe structures are at the base of the entire industry. In the industry structure, heat and vibration are transmitted in each pipe. The minimum distance between each pipe is significant to the security. The assembly error and the deformation of the pipeline positions after multiple runs are significant problems. The reconstruction of the multi-pipe system is a critical technical difficulty in the complex tube system. In this paper, a new method for the multi-pipes structure inspection is presented. Images of the tube system are acquired from several positions. The photogrammetry technology calculates positions, and the necessary coordination of the structure is reconstructed. A convolution neural network is utilized to detect edges of tube-features. The new algorithm for tube identification and reconstruction is presented to extract the tube feature in the image and reconstruct the 3D parameters of all tubes in a multi-pipes structure. The accuracy of the algorithm is verified by simulation experiments. An actual engine of the aircraft is measured to verify the proposed method.

scholarly journals Cost Analysis of a Novel Method for Ecological Compensation—A Study of the Translocation of Dead Wood

2021 ◽  
Vol 13 (11) ◽  
pp. 6075
Author(s):  
Ola Lindroos ◽  
Malin Söderlind ◽  
Joel Jensen ◽  
Joakim Hjältén
Keyword(s):  
Cost Efficiency ◽  
Dead Wood ◽  
Road Transportation ◽  
Ecological Value ◽  
Ecological Compensation ◽  
Work Time ◽  
Delivery Times ◽  
Novel Method ◽  
Cost Efficient ◽  
The Cost

Translocation of dead wood is a novel method for ecological compensation and restoration that could, potentially, provide a new important tool for biodiversity conservation. With this method, substrates that normally have long delivery times are instantly created in a compensation area, and ideally many of the associated dead wood dwelling organisms are translocated together with the substrates. However, to a large extent, there is a lack of knowledge about the cost efficiency of different methods of ecological compensation. Therefore, the costs for different parts of a translocation process and its dependency on some influencing factors were studied. The observed cost was 465 SEK per translocated log for the actual compensation measure, with an additional 349 SEK/log for work to enable evaluation of the translocation’s ecological results. Based on time studies, models were developed to predict required work time and costs for different transportation distances and load sizes. Those models indicated that short extraction and insertion distances for logs should be prioritized over road transportation distances to minimize costs. They also highlighted a trade-off between costs and time until a given ecological value is reached in the compensation area. The methodology used can contribute to more cost-efficient operations and, by doing so, increase the use of ecological compensation and the benefits from a given input.

A Novel Method for Preparation of Antimicrobial Cellulose Textile by Surface Carbamatization and N-Chlorination

2011 ◽  
Vol 399-401 ◽  
pp. 1088-1092
Author(s):  
Liu Yang Wang ◽  
Fang Ying Wang ◽  
Biao Bing Wang
Keyword(s):  
Nitrogen Content ◽  
Shake Flask ◽  
Antimicrobial Activities ◽  
Sodium Hypochlorite Solution ◽  
E Coli ◽  
Cellulose Fabric ◽  
Carbamate Group ◽  
Novel Method ◽  
Cellulose Fabrics ◽  
Temperature Time

Surface carbamatization of cellulose fabric was accomplished through co-heating with urea. The carbamate group on textile surface was transformed to N-chlorocarbamate after exposure to sodium hypochlorite solution. Effect of carbamatization temperature, time on Nitrogen content of fabrics was investigated, and the effect of bleach technology on chlorine content on fabrics was also studied. Using shake flask method antimicrobial activities against E. coli and S. aureus were studied. The results showed that when carbamatization temperature was 138°C and the time was 3 hours, nitrogen content of cellulose fabric was the highest: 1.47%. Antibacterial ability of cellulose fabrics with 0.81mg/g chlorine against E. coli and S. aureus was 99.9999% and 100%, respectively.

scholarly journals Color Metallography

2005 ◽  
Vol 13 (6) ◽  
pp. 22-27 ◽  
Author(s):  
George F. Vander Voort
Keyword(s):  
Digital Imaging ◽  
Color Images ◽  
Vast Number ◽  
Human Eye ◽  
Black And White ◽  
Color Metallography ◽  
The Cost

Color has historically seen limited use in metallography, mainly due to the cost of film and prints and the difficulty and cost of reproducing images in publications. However, with the growth of digital imaging, capturing color images is much simpler and cheaper. Also, printing images in color is inexpensive for in-house reports, and can be distributed cheaply on CDs, although reproduction in journals is still expensive. Color does have many advantages over black and white. First, the human eye is sensitive to only about forty shades of gray from white to black, but is sensitive to a vast number of colors. Tint etchants reveal features in the microstructure that often cannot be revealed using standard black and white etchants.

Review of recent developments on improvement of estimated regulatory documentation for road works

2021 ◽  
Vol 2021 (23) ◽  
pp. 31-42
Author(s):  
Anatolii Tsynka ◽  
◽  
Andrii Hrinchuk ◽  
Ivan Rakovych ◽  
◽  
...  
Keyword(s):  
Work Performance ◽  
New Technologies ◽  
Operation Time ◽  
Road Construction ◽  
Labor Costs ◽  
The Road ◽  
Regulatory Documents ◽  
Road Work ◽  
Investment Process ◽  
The Cost

Introduction. In the modern conditions of the pricing system in the construction of Ukraine, the key value have the estimated norms, which are presented in separate collections, the main purpose of which is to determine the standard quantity of resources required to perform a particular type of work as a basis for the transition to the cost indicators. In order to determine the cost of road work objectively and accurately, it is essential that the indicators of estimated norms meet the requirements of regulatory documents and modern methods of execution of road works.Problematics. In connection with the active improvement of the regulatory base of the road construction industry, permanent upgrade of road organizations technique fleet, the introduction of new technologies and materials in construction and operation of roads, improving the methods of work there is a discrepancy between the existing resource element of estimated norms and the actual conditions of work in terms of built-up labor costs, the operation time of machines and mechanisms and the range of materials. Accordingly, it affects the reliability and accuracy of determining the cost of road works.Purpose. Improvement and harmonization with actual working conditions and requirements of regulatory documents of industry regulatory and estimate base of resource elementary estimated norms for work performed during construction, reconstruction, repair and operational maintenance of roads and bridges to ensure reliable and valid technical, economic and estimated calculations.Materials and methods. During work performance the analysis of the regulatory documentation, establishing the requirements to the technology of road works and materials has been carried out. A number of chronometric observations with the measurement of time of road works in full-scale conditions was carried out. The obtained data were summarized, averaged and on their basis the indicators of the resource element of the estimated norms were estimated.Results. A review of the main amendments and additions, which came into force after the approval of Amendment No 2 SOU 42.1-37641918-035:2018 [1] and Amendment No 2 SOU 42.1-37641918-071:2018 [2], which were developed to improve the regulatory-estimate base for the calculation of road works, taking into account the requirements and provisions of existing regulatory documents taking into account the current state of scientific and technological progress in the road sector.Conclusions. The above analysis of the amendments that came into force with the approval of [1]. and [2] will systematize the innovations provided by the requirements of current regulatory documents on the calculation of the cost of road works and the development of relevant documents at all stages of the investment process, planning and organization of road works, as well as the writing-off of material resources. Review and systematization of recent improvements in the estimated regulatory documents for road works will provide an opportunity to optimally plan the use of available material, labor and financial resources, often limited.Keywords: public road, operational maintenance, investment documentation, overhaul and current repairs, machine, mechanism, regulatory document, resource element estimate norm, Standard of Organization of Ukraine.

Preliminary Estimates of Costs of Foodborne Disease in the United States

1989 ◽  
Vol 52 (8) ◽  
pp. 595-601 ◽  
Author(s):  
EWEN C. D. TODD
Keyword(s):  
United States ◽  
Food Industry ◽  
The United States ◽  
Control Measures ◽  
Effective Control ◽  
Foodborne Diseases ◽  
Hemorrhagic Colitis ◽  
E Coli ◽  
The Cost ◽  
Total Impact

Although the full economic impact of foodborne diseases has yet to be measured, preliminary studies show that the cost of illness, death, and business lost is high indeed. This impact is probably greatest in developing countries, but few facts are known. For the United States, preliminary estimates are 12.6 million cases costing $8.4 billion. These may seem excessive but other authors have postulated even higher case and dollar figures. Microbiological diseases (bacterial and viral) represent 84% of the United States' costs, with salmonellosis and staphylococcal intoxication being the most economically important diseases (annually $4.0 billion and $1.5 billion, respectively). Other costly types of illnesses are toxoplasmosis ($445 million), listeriosis ($313 million), campylobacteriosis ($156 million), trichinosis ($144 million), Clostridium perfringens enteritis ($123 million), and E. coli infections including hemorrhagic colitis ($223 million). Botulism has a high cost per case ($322,200), but its total impact is only $87 million because relatively few cases occur (270). This is because the food industry has been able to introduce effective control measures. Salmonellosis, however, is much more widespread (2.9 million cases) and affects all sectors of the food industry.

scholarly journals Embedding Undersampling Rotation Forest for Imbalanced Problem

2018 ◽  
Vol 2018 ◽  
pp. 1-15 ◽  
Author(s):  
Huaping Guo ◽  
Xiaoyu Diao ◽  
Hongbing Liu
Keyword(s):  
Imbalanced Data ◽  
Feature Space ◽  
Original Data ◽  
Training Set ◽  
Data Set ◽  
Minority Class ◽  
Rotation Forest ◽  
Novel Method ◽  
Individual Classifier ◽  
The Cost

Rotation Forest is an ensemble learning approach achieving better performance comparing to Bagging and Boosting through building accurate and diverse classifiers using rotated feature space. However, like other conventional classifiers, Rotation Forest does not work well on the imbalanced data which are characterized as having much less examples of one class (minority class) than the other (majority class), and the cost of misclassifying minority class examples is often much more expensive than the contrary cases. This paper proposes a novel method called Embedding Undersampling Rotation Forest (EURF) to handle this problem (1) sampling subsets from the majority class and learning a projection matrix from each subset and (2) obtaining training sets by projecting re-undersampling subsets of the original data set to new spaces defined by the matrices and constructing an individual classifier from each training set. For the first method, undersampling is to force the rotation matrix to better capture the features of the minority class without harming the diversity between individual classifiers. With respect to the second method, the undersampling technique aims to improve the performance of individual classifiers on the minority class. The experimental results show that EURF achieves significantly better performance comparing to other state-of-the-art methods.

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