Planting mixed forests of European beech and conifers: Variations in microbial responses with site conditions
AbstractTree - soil interactions depend on environmental context. Plantations of trees may impact soil microorganisms more strongly under unfavorable environmental conditions, compromising long-term ecosystem services. To contextually understand the effects of tree species composition on soil microorganisms, we quantified structural and functional responses of soil microorganisms to forest types across environmental gradients using substrate-induced respiration and phospholipid fatty acid analyses. Five forest types were studied including pure stands of native European beech (Fagus sylvatica), range expanding Norway spruce (Picea abies), and non-native Douglas-fir (Pseudotsuga menziesii), as well as the two conifer - beech mixtures. We found that microbial functioning strongly depends on environmental conditions, in particular on soil nutrients. At nutrient-poor sites, both pure and mixed coniferous forests, but especially Douglas-fir forests, stressed soil microorganisms compared to beech forests. By contrast, microbial structure and functional indicators in beech forests varied little with site conditions, likely because beech provided high amounts of root-derived resources for microbial growth. The results indicate that, at nutrient-poor sites, long-term effects of planting exotic Douglas-fir on ecosystem functioning need further attention, but planting Douglas-fir at nutrient-rich sites may be of little concern from the perspective of microbial communities. Overall, the results point to the importance of root-derived resources in determining the structure and functioning of soil microbial communities, and document the sensitivity of soil microorganisms to planting tree species that may differ in the provisioning of these resources.