scholarly journals Sucrose transport and Sucrose Synthase activity control the source-sink relationship for biomass and grain yield in rice

2020 ◽  
Author(s):  
Jyotirmaya Mathan ◽  
Anuradha Singh ◽  
Aashish Ranjan

AbstractThe source-sink relationship is key to overall crop performance. Detailed understanding of the factors that determine source-sink dynamics is imperative for the balance of biomass and grain yield in a crop plant. We investigated the differences in the source-sink relationship between a cultivated rice Oryza sativa cv. Nipponbare and a wild rice Oryza australiensis that show striking differences in biomass and grain yield. The wild rice, accumulating higher biomass, was not only photosynthetically efficient but also had efficient sucrose export from leaves. However, sucrose mobilization to the grains was limited due to impaired vasculature and sucrose transporter functions at the reproductive tissues of the wild rice. High cleavage activity of Sucrose Synthase followed by higher expression of Cellulose Synthase genes in the wild rice stem efficiently utilized photosynthates for the synthesis of structural carbohydrates, resulting in high biomass. In contrast, the source-sink relationship favored high grain yield in Nipponbare via accumulation of transitory starch in the stem, due to higher expression of starch biosynthetic genes, which is mobilized to panicles at the grain filling stage. Thus, sucrose transport along with functions of key sugar metabolic enzymes explained the differences in the source-sink relationship between the selected cultivated and wild rice.

Author(s):  
Jyotirmaya Mathan ◽  
Anuradha Singh ◽  
Aashish Ranjan

Abstract The source-sink relationship is key to overall crop performance. Detailed understanding of the factors that determine source-sink dynamics is imperative for the balance of biomass and grain yield in crop plants. We investigated the differences in the source-sink relationship between a cultivated rice Oryza sativa cv. Nipponbare and a wild rice Oryza australiensis that show striking differences in biomass and grain yield. Oryza australiensis, accumulating higher biomass, not only showed higher photosynthesis per unit leaf area but also exported more sucrose from leaves than Nipponbare. However, grain features and sugar levels suggested limited sucrose mobilization to the grains in the wild rice due to vasculature and sucrose transporter functions. Low cell wall invertase activity and high sucrose synthase cleavage activity followed by higher expression of cellulose synthase genes in Oryza australiensis stem utilized photosynthates preferentially for the synthesis of structural carbohydrates, resulting in high biomass. In contrast, the source-sink relationship favored high grain yield in Nipponbare via accumulation of transitory starch in the stem, due to higher expression of starch biosynthetic genes, which is mobilized to panicles at the grain filling stage. Thus, vascular features, sucrose transport, and functions of sugar metabolic enzymes explained the differences in the source-sink relationship between Nipponbare and Oryza australiensis.


2016 ◽  
Vol 36 (5) ◽  
Author(s):  
李玉 LI Yu ◽  
丁焕新 DING Huanxin ◽  
丁秀文 DING Xiuwen ◽  
殷毅凡 YIN Yifan ◽  
孙影 SUN Ying ◽  
...  

2006 ◽  
Vol 57 (2) ◽  
pp. 227 ◽  
Author(s):  
Daniel F. Calderini ◽  
M. P. Reynolds ◽  
G. A. Slafer

Source limitation during grain filling is important for both management and breeding strategies of grain crops. There is little information on the sensitivity of grain weight of temperate cereals to variations in source–sink ratios, and no studies are available on the comparative behaviour of temperate cereals growing together in the same experiment. The objective of the current study was to evaluate, under field conditions, the response of grain weight to different source–sink ratios during grain filling in high-yielding cultivars of bread wheat, durum wheat, and triticale at 2 contrasting locations. Two experiments were carried out at C. Obregon and El Batan in Mexico. In each location, 6 genotypes (2 bread wheat, 2 durum wheat, 2 triticale) were evaluated. A week after anthesis, 2 source–sink (control and halved spikes) treatments were imposed. Location and genotype significantly (P < 0.01) affected grain yield and components. Significant grain weight increases (P < 0.05) were found only in 2 cases in El Batan. The highest response of 17% was found in triticale, with less than 10% in most of the other genotypes. The effect of genotype and location is discussed.


Biology ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 42
Author(s):  
Cong Zhang ◽  
Bangyou Zheng ◽  
Yong He

Improving plant net photosynthetic rates and accelerating water-soluble carbohydrate accumulation play an important role in increasing the carbon sources for yield formation of wheat (Triticum aestivum L.). Understanding and quantify the contribution of these traits to grain yield can provide a pathway towards increasing the yield potential of wheat. The objective of this study was to identify kernel weight gap for improving grain yield in 15 winter wheat genotypes grown in Shandong Province, China. A cluster analysis was conducted to classify the 15 wheat genotypes into high yielding (HY) and low yielding (LY) groups based on their performance in grain yield, harvest index, photosynthetic rate, kernels per square meter, and spikes per square meter from two years of field testing. While the grain yield was significantly higher in the HY group, its thousand kernel weight (TKW) was 8.8% lower than that of the LY group (p < 0.05). A structural equation model revealed that 83% of the total variation in grain yield for the HY group could be mainly explained by TKW, the flag leaf photosynthesis rate at the grain filling stage (Pn75), and flag leaf water-soluble carbohydrate content (WSC) at grain filling stage. Their effect values on yield were 0.579, 0.759, and 0.444, respectively. Our results suggest that increase of flag leaf photosynthesis and WSC could improve the TKW, and thus benefit for developing high yielding wheat cultivars.


2020 ◽  
Vol 48 (5) ◽  
pp. 2127-2137
Author(s):  
Matthew J. Paul ◽  
Amy Watson ◽  
Cara A. Griffiths

The domestication and breeding of crops has been a major achievement for mankind enabling the development of stable societies and civilisation. Crops have become more productive per unit area of cultivated land over the course of domestication supporting a current global population of 7.8 billion. Food security crops such as wheat and maize have seen large changes compared with early progenitors. Amongst processes that have been altered in these crops, is the allocation of carbon resources to support larger grain yield (grain number and size). In wheat, reduction in stem height has enabled diversion of resources from stems to ears. This has freed up carbon to support greater grain yield. Green revolution genes responsible for reductions in stem height are known, but a unifying mechanism for the active regulation of carbon resource allocation towards and within sinks has however been lacking. The trehalose 6-phosphate (T6P) signalling system has emerged as a mechanism of resource allocation and has been implicated in several crop traits including assimilate partitioning and improvement of yield in different environments. Understanding the mode of action of T6P through the SnRK1 protein kinase regulatory system is providing a basis for a unifying mechanism controlling whole-plant resource allocation and source-sink interactions in crops. Latest results show it is likely that the T6P/SnRK1 pathway can be harnessed for further improvements such as grain number and grain filling traits and abiotic stress resilience through targeted gene editing, breeding and chemical approaches.


Author(s):  
Adnan Al-ghawry ◽  
Attila Yazar ◽  
Mustafa Unlu ◽  
Celaleddin Barutcular ◽  
Yeşim Bozkurt Çolak

Abstract A field experiment was carried out to evaluate the effect of different conventional and supplemental irrigation strategies on leaf stomatal conductance (gs) and chlorophyll content (SPAD) yield and irrigation water productivity (IWP) of wheat using sprinkler line source in 2014 and 2015 in the Mediterranean region. The irrigation strategies were, supplemental irrigation (SI) during flowering and grain filling (SIFG), SI during grain filling (SIG), SI during flowering (SIF) and conventional irrigation (CI). These strategies were conducted under four irrigation levels 25, 50, 75, 100% and a rain-fed as control. The results indicated that CI100 and CI75 produced the greater grain yield and IWP, respectively. CI100 resulted in the increased chlorophyll content by 8.8% over rain-fed. The results confirmed that the SPAD and stomatal conductance values were not equally sensitive to water stress during growth stages. The wheat crop suffered a greater SPAD and gs reductions when the water stress occurred during the grain filling stage (SIF strategy) compared to other strategies, which means that the grain filling stage is more sensitive and effective to decrease the yield of winter wheat. The higher grain yields were achieved when the seasonal mean gs reached 207.4 mmol/m2s in CI and 169.2 mmol/m2s in SI, and the stomatal closure responded well to low, moderate and severe drought treatments. The leaf stomatal conductance (gs) was correlated linearly with grain yield. These relations could be used as a physiological indicator to evaluate water stress effect on the growth and productivity of wheat.


2010 ◽  
Vol 61 (10) ◽  
pp. 852 ◽  
Author(s):  
Heping Zhang ◽  
Neil C. Turner ◽  
Michael L. Poole

Grain yield depends on the number of grains per unit area (sink) and the availability of assimilates (source) to fill these grains. The aim of the current work was to determine whether wheat yield in the high-rainfall zone of south-western Australia is limited in current cultivars by the size of the sink or by the assimilates available for grain filling. Three wheat cultivars (Calingiri, Chara and Wyalkatchem) and two breeding lines (HRZ216 and HRZ203) were grown in four replicates in the field from 2005 to 2007. Dry matter and water soluble carbohydrates (WSC) at anthesis and maturity were measured and used to determine the source and sink balance of the crop. In 2007, three further treatments were applied to manipulate the sink–source relationships: (i) spikelets were removed on main stems to increase the source : sink ratio; (ii) incoming solar radiation was reduced by 40% by shading after anthesis to reduce the availability of assimilates to grains; and (iii) supplemental irrigation was used to maintain the capacity for photosynthesis by an improved water supply during grain filling. The source–sink balance of the crops showed that the potential source was 25% greater than the actual grain yield in average and above-average seasons (2005 and 2007), suggesting that sink size, represented by the number of grain per unit area, was a limiting factor to yield potential. However, the source may have become a limiting factor in a drought season (2006). The grain yield increased with increased number of grains/m2 and kernel weight remained relatively stable even when grain number increased from 7000 to 16 000 per m2. The removal of half of the spikelets on the main stem did not increase kernel mass of the remaining grains and an additional 33 mm of irrigation water did not increase grain yield, but significantly (P < 0.05) increased WSC left in stems and leaf sheaths at maturity. Shading after anthesis did not significantly reduce grain yield of the current cultivars Calingiri and Wyalkatchem, but it reduced grain yield by 23–25% (P < 0.05) in Chara and HRZ203. The source–sink balance over three seasons and three independent experiments in 2007 suggested that the yield of the current wheat cultivars is more sink- than source-limited and that breeding wheat with a larger sink size than in the current cultivars may lift the yield potential of wheat in the high-rainfall zone of south-western Australia.


MAUSAM ◽  
2021 ◽  
Vol 67 (1) ◽  
pp. 205-222
Author(s):  
RAM NIWAS ◽  
M. L. KHICHAR

Wheat and mustard crop is highly vulnerable, particularly in the semi-arid and arid regions of India. The climate is warming through the processes such as CO2 and changed pattern of temperature and precipitation resulting in heat and drought stresses, respectively. The effect of increasing temperature during grain filling stage of wheat causes substantial reduction in grain yield. The effect of low temperature (frost) during podding and seed development stage in mustard causes freezing injury in seeds and sizable reduction in seed yield.  In this review paper response of wheat and mustard crop to weather extremes and management practices such as time of sowing, selection of resistance cultivars, mulching, seed priming, foliar spray of salts, use of extra irrigation water, foliar spray of micronutrients, sprinkler, wind barrier etc. to mitigate the temperature and moisture stress effect on the productivity of wheat and mustard crop  have been discussed. Above ground dry weight of wheat and its rate decreased with increasing water stress at each stage. The averaged values of damage threshold temperatures  compiled from the literature were 31 °C for flowering and 35 °C for grain filling of wheat. Changes in average daily maximum temperature during flowering and grain filling had a negative effect on grain yield of 518 kg/ha and 1140 kg/ha, respectively for every 1 degree increase in average maximum temperature in South Australia. Temperature rise would be most harmful for the crop in eastern region, followed by central and northern India, where winter season temperature is comparatively higher than northern region. Rainfed mustard was less vulnerable to temperature rise in northern India as compared to other two central and eastern India. Rise in atmospheric temperature reduced leaf area index, grain number as well as weight of grains which was in turn reflected in yield of mustard crop. Seed yield reduction occurred by low water availability during stem elongation, flowering and pod development in mustard. Priming with moringa water extract and ascorbate substantially improved the tissue water status, membrane stability, gas exchange, water productivity of the plant. Late sown wheat crop faces high temperature stress during ripening phase. Delayed sowing reduces the tillering period and hot weather during critical period of grain filling lead to forced maturity thereby reduces the grain yield. Application of mulches in wheat produced higher grain yield over without much wheat. Organic mulches provided better soil water status and improved plant canopy in terms of biomass, root growth, leaf area index and grain yield as compared to inorganic mulch. The foliar spray of KNO3 (0.5%) at 50 per cent flowering stage, 1.0 per cent KNO3 during anthesis stage, 2.5 mM of arginine, spray of zinc, extra irrigation water during grain filling stage increased the productivity of wheat under high temperature stress.  Light irrigation in mustard crop one day before frost occurrence protects from frost damage by improving heat transfer and heat capacity.  Plastic mulch raises the surface temperature of the soil nearly 10 °C over   bare soil.  Smoke particles are usually less than 1 µm in size, reflect visible radiation but trap the long wave radiation and so are effective in preventing rapid cooling of surface near ground. Mixing air and liquid materials in the right proportion to create many small bubbles is the secret to generate foam with low thermal conductivity. Organic mulches (straw and saw dust) provided better soil water status over ash mulch.  


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