scholarly journals Elevated temperature inhibits SARS-CoV-2 replication in respiratory epithelium independently of the induction of IFN-mediated innate immune defences

2020 ◽  
Author(s):  
Vanessa Herder ◽  
Kieran Dee ◽  
Joanna K. Wojtus ◽  
Daniel Goldfarb ◽  
Christoforos Rozario ◽  
...  
Keyword(s):  
Elevated Temperature ◽  
International Health ◽  
Tissue Temperature ◽  
Temperature Elevation ◽  
Microbial Infection ◽  
Health Crisis ◽  
Infection And Inflammation ◽  
Rna Accumulation ◽  
Natural Tissue ◽  
Immune Defences

AbstractThe pandemic spread of SARS-CoV-2, the etiological agent of COVID-19, represents a significant and ongoing international health crisis. A key symptom of SARS-CoV-2 infection is the onset of fever, with a hyperthermic temperature range of 38 to 41°C. Fever is an evolutionarily conserved host response to microbial infection and inflammation that can influence the outcome of viral pathogenicity and regulation of host innate and adaptive immune responses. However, it remains to be determined what effect elevated temperature has on SARS-CoV-2 tropism and replication. Utilizing a 3D air-liquid interface (ALI) model that closely mimics the natural tissue physiology and cellular tropism of SARS-CoV-2 infection in the respiratory airway, we identify tissue temperature to play an important role in the regulation of SARS-CoV-2 infection. We show that temperature elevation induces wide-spread transcriptome changes that impact upon the regulation of multiple pathways, including epigenetic regulation and lncRNA expression, without disruption of general cellular transcription or the induction of interferon (IFN)-mediated antiviral immune defences. Respiratory tissue incubated at temperatures >37°C remained permissive to SARS-CoV-2 infection but severely restricted the initiation of viral transcription, leading to significantly reduced levels of intraepithelial viral RNA accumulation and apical shedding of infectious virus. To our knowledge, we present the first evidence that febrile temperatures associated with COVID-19 inhibit SARS-CoV-2 replication. Our data identify an important role for temperature elevation in the epithelial restriction of SARS-CoV-2 that occurs independently of the induction of canonical IFN-mediated antiviral immune defences and interferon-stimulated gene (ISG) expression.

scholarly journals Elevated temperature inhibits SARS-CoV-2 replication in respiratory epithelium independently of IFN-mediated innate immune defenses

PLoS Biology ◽  
2021 ◽  
Vol 19 (12) ◽  
pp. e3001065
Author(s):  
Vanessa Herder ◽  
Kieran Dee ◽  
Joanna K. Wojtus ◽  
Ilaria Epifano ◽  
Daniel Goldfarb ◽  
...  
Keyword(s):  
Elevated Temperature ◽  
Three Dimensional ◽  
International Health ◽  
Tissue Temperature ◽  
Host Responses ◽  
Microbial Infection ◽  
Health Crisis ◽  
Immune Defenses ◽  
Natural Tissue ◽  
Air Liquid Interface

The pandemic spread of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), the etiological agent of Coronavirus Disease 2019 (COVID-19), represents an ongoing international health crisis. A key symptom of SARS-CoV-2 infection is the onset of fever, with a hyperthermic temperature range of 38 to 41°C. Fever is an evolutionarily conserved host response to microbial infection that can influence the outcome of viral pathogenicity and regulation of host innate and adaptive immune responses. However, it remains to be determined what effect elevated temperature has on SARS-CoV-2 replication. Utilizing a three-dimensional (3D) air–liquid interface (ALI) model that closely mimics the natural tissue physiology of SARS-CoV-2 infection in the respiratory airway, we identify tissue temperature to play an important role in the regulation of SARS-CoV-2 infection. Respiratory tissue incubated at 40°C remained permissive to SARS-CoV-2 entry but refractory to viral transcription, leading to significantly reduced levels of viral RNA replication and apical shedding of infectious virus. We identify tissue temperature to play an important role in the differential regulation of epithelial host responses to SARS-CoV-2 infection that impact upon multiple pathways, including intracellular immune regulation, without disruption to general transcription or epithelium integrity. We present the first evidence that febrile temperatures associated with COVID-19 inhibit SARS-CoV-2 replication in respiratory epithelia. Our data identify an important role for tissue temperature in the epithelial restriction of SARS-CoV-2 independently of canonical interferon (IFN)-mediated antiviral immune defenses.

The Role of Formyl Peptide Receptors in Microbial Infection and Inflammation

2003 ◽  
Vol 2 (1) ◽  
pp. 83-93 ◽  
Author(s):  
Yingying Le ◽  
Ronghua Sun ◽  
Guoguang Ying ◽  
Pablo Iribarren ◽  
Ji Wang
Keyword(s):  
Microbial Infection ◽  
Peptide Receptors ◽  
Formyl Peptide Receptors ◽  
Formyl Peptide ◽  
Infection And Inflammation

Synergising International Public Health Law and International Disaster Law

2020 ◽  
pp. 1-11
Author(s):  
Pratik DIXIT
Keyword(s):  
Public Health ◽  
International Health ◽  
Health Law ◽  
Global Public Health ◽  
Public Health Law ◽  
Health Crisis ◽  
Response Strategies ◽  
Public Health Crisis ◽  
International Public Health ◽  
Health Regulations

There is no time more opportune to review the workings of the International Health Regulations (IHR) than the present COVID-19 crisis. This article analyses the theoretical and practical aspects of international public health law (IPHL), particularly the IHR, to argue that it is woefully unprepared to protect human rights in times of a global public health crisis. To rectify this, the article argues that the IHR should design effective risk reduction and response strategies by incorporating concepts from international disaster law (IDL). Along similar lines, this article suggests that IDL also has a lot to learn from IPHL in terms of greater internationalisation and institutionalisation. Institutionalisation of IDL on par with IPHL will provide it with greater legitimacy, transparency and accountability. This article argues that greater cross-pollination of ideas between IDL and IPHL is necessary in order to make these disciplines more relevant for the future.

The Effects of Superficial Heating before 1-MHz Ultrasound on Tissue Temperature

2003 ◽  
Vol 12 (2) ◽  
pp. 95-103 ◽  
Author(s):  
William R. Holcomb ◽  
Chris Blank
Keyword(s):  
Athletic Training ◽  
Repeated Measures ◽  
Outcome Measure ◽  
Tissue Temperature ◽  
Temperature Elevation ◽  
Main Outcome Measure ◽  
Cooling Period ◽  
Dependent Variables ◽  
Within Subjects ◽  
Independent Variable

Context:Ultrasound significantly raises tissue temperature, but the time of temperature elevation is short.Objective:To assess the effectiveness of superficial preheating on temperature elevation and decline when using ultrasound.Design:Within-subjects design to test the independent variable, treatment condition; repeated-measures ANOVAs to analyze the dependent variables, temperature elevation and decline.Setting:Athletic training laboratory.Intervention:Temperature at a depth of 3.75 cm was measured during ultrasound after superficial heating and with ultrasound alone.Subjects:10 healthy men.Main Outcome Measure:Temperature was recorded every 30 s during 15 min of ultrasound and for 15 min afterward.Results:Temperature elevation with ultrasound was significantly greater with preheating (4.0 ± 0.21 °C) than with ultrasound alone (3.0 ± 0.22 °C). Temperature decline was not significantly different between preheating and ultrasound alone.Conclusions:Superficial preheating significantly increases temperature elevation but has no effect on temperature decline during a 15-min cooling period.

scholarly journals The New Reality of Bangladesh: A Critical Analysis of New Normal Challenges and Opportunities

2020 ◽  
Vol 1 (2) ◽  
pp. 126-146
Author(s):  
Umme Sayeda
Keyword(s):  
Policy Development ◽  
Human Life ◽  
International Health ◽  
Analysis Tool ◽  
Governance System ◽  
Health Crisis ◽  
New Normal ◽  
Secondary Sources ◽  
Grounded Theory Method ◽  
Challenges And Opportunities

The post-COVID-19 new normal will arise as a game-changer in the policy-making of the world states. Accordingly, this article highlights the post-pandemic Bangladesh that should integrate biology affirmatively in the policy development procedures to reshape the new normal challenges as opportunities. The grounded theory method is adopted as a quantitative analysis tool relying on the secondary sources of data to portray the significance of biopolitics as political rationality in new norm Bangladesh. The researcher has used the neo-realism approach to develop the ‘Biopolitical Rationale Theory’, which uncovers how evolving neo-realist security demands the prioritization of biopolitics in every sphere of decision making for governing the post-pandemic new standard of existence. The 2020 corona outbreak proved that human life and the environment are the ultimate means of survival rather than the traditional security arrangements and extreme economic growth which are inhumane (rationality of death and militarization), unhygienic, and destructive to the environment (exploitation of nature is profitable). The article recommends some alternative new normal policies such as non-discriminative health policy, bordering in line with International Health Regulations (IHR), digitalization with better cybersecurity, virtualization of the tourist industry (application of Extended Reality), application of Career Resilience (CR), and Strategic Flexibility Analysis tools in the re-employment and career development, greening the economy, special arrangements for emergency health crisis and undertaking actions considering the environment as a remedy rather than a crisis. The review research concludes that the inclusion of biopolitics in the Bangladesh governance system can redesign the challenges of new normal as new opportunities. But the reshaping of such a new reality will itself prevail as a considerable challenge for Bangladesh.

scholarly journals Adapting French COVID-19 vaccination campaign duration to variant dissemination

2021 ◽  
Author(s):  
Simon Pageaud ◽  
Nicolas Ponthus ◽  
Romain Gauchon ◽  
Catherine Pothier ◽  
Christophe Rigotti ◽  
...  
Keyword(s):  
Disease Transmission ◽  
Vaccination Campaign ◽  
Virus Transmission ◽  
International Health ◽  
Vaccination Strategy ◽  
Health Crisis ◽  
Population Immunity ◽  
Best Response ◽  
Rapid Dissemination ◽  
Vaccination Campaigns

Background The outbreak of SARS-CoV-2 virus has caused a major international health crisis with serious consequences in terms of public health and economy. In France, two lockdown periods were decided in 2020 to avoid the saturation of intensive care units (ICU) and an increase in mortality. The rapid dissemination of variant SARS-CoV-2 VOC 202012/01 has strongly influenced the course of the epidemic. Vaccines have been rapidly developed. Their efficacy against the severe forms of the disease has been established, and their efficacy against disease transmission is under evaluation. The aim of this paper is to compare the efficacy of several vaccination strategies in the presence of variants in controlling the COVID-19 epidemic through population immunity. Methods An agent-based model was designed to simulate with different scenarios the evolution of COVID-19 pandemic in France over 2021 and 2022. The simulations were carried out ignoring the occurrence of variants then taking into account their diffusion over time. The expected effects of three Non-Pharmaceutical Interventions (Relaxed-NPI, Intensive-NPI, and Extended-NPI) to limit the epidemic extension were compared. The expected efficacy of vaccines were the values recently estimated in preventing severe forms of the disease (75% and 94%) for the current used vaccines in France (Pfizer-BioNTech and Moderna since January 11, 2021, and AstraZeneca since February 2, 2021). All vaccination campaigns reproduced an advanced age-based priority advised by the Haute Autoritć de Santć. Putative reductions of virus transmission were fixed at 0, 50, 75 and 90%. The effects of four vaccination campaign durations (6-month, 12-month, 18-month and 24-month) were compared. Results In the absence of vaccination, the presence of variants led to reject the Relaxed-NPI because of a high expected number of deaths (170 to 210 thousands) and the significant overload of ICUs from which 35 thousand patients would be deprived. In comparison with the situation without vaccination, the number of deaths was divided by 7 without ICU saturation with a 6-month vaccination campaign. A 12-month campaign would divide the number of deaths by 3 with Intensive-NPI and by 6 with Extended-NPI (the latter being necessary to avoid ICU saturation). With 18-month and 24-month vaccination campaigns without Extended-NPI, the number of deaths and ICU admissions would explode. Conclusion Among the four compared strategies the 6-month vaccination campaign seems to be the best response to changes in the dynamics of the epidemic due to the variants. The race against the COVID-19 epidemic is a race of vaccination strategy. Any further vaccination delay would increase the need of strengthened measures such as Extended-NPI to limit the number of deaths and avoid ICU saturation.

scholarly journals On the interactions of the receptor-binding domain of SARS-CoV-1 and SARS-CoV-2 spike proteins with monoclonal antibodies and the receptor ACE2

2020 ◽  
Author(s):  
Carolina Corrêa Giron ◽  
Aatto Laaksonen ◽  
Fernando L. Barroso da Silva
Keyword(s):  
Monoclonal Antibodies ◽  
Receptor Binding ◽  
International Health ◽  
Binding Domain ◽  
Clinical Aspects ◽  
Receptor Binding Domain ◽  
Health Crisis ◽  
Structural Modifications ◽  
Constant Ph ◽  
Promising Target

ABSTRACTA new betacoronavirus named SARS-CoV-2 has emerged as a new threat to global health and economy. A promising target for both diagnosis and therapeutics treatments of the new disease named COVID-19 is the coronavirus (CoV) spike (S) glycoprotein. By constant-pH Monte Carlo simulations and the PROCEEDpKa method, we have mapped the electrostatic epitopes for four monoclonal antibodies and the angiotensin-converting enzyme 2 (ACE2) on both SARS-CoV-1 and the new SARS-CoV-2 S receptor binding domain (RBD) proteins. We also calculated free energy of interactions and shown that the S RBD proteins from both SARS viruses binds to ACE2 with similar affinities. However, the affinity between the S RBD protein from the new SARS-CoV-2 and ACE2 is higher than for any studied antibody previously found complexed with SARS-CoV-1. Based on physical chemical analysis and free energies estimates, we can shed some light on the involved molecular recognition processes, their clinical aspects, the implications for drug developments, and suggest structural modifications on the CR3022 antibody that would improve its binding affinities for SARS-CoV-2 and contribute to address the ongoing international health crisis.

scholarly journals Analisis Pergeseran Makna Partisipasi Politik Perempuan Berdasarkan Karakteristik Feminitas dan Maskulinitas dalam Kepemimpinan Perempuan: Studi Kasus Penanganan COVID-19 di Jerman, Selandia Baru, dan Taiwan

2020 ◽  
Vol 1 (2) ◽  
pp. 91-108
Author(s):  
Bella Aprilia ◽  
Florencia Maria Surya ◽  
Mentari Svarna Pertiwi
Keyword(s):  
Prime Minister ◽  
International Health ◽  
Political Leadership ◽  
Female Leaders ◽  
Health Crisis ◽  
The World ◽  
Femininity And Masculinity ◽  
Angela Merkel ◽  
New Perspective ◽  
The Impact

 ABSTRAK                COVID-19 merupakan salah satu permasalahan global yang sedang dihadapi oleh banyak negara di seluruh dunia. Selain menyebabkan krisis kesehatan internasional, pandemi COVID-19 juga mengancam dinamika kehidupan masyarakat secara luas, baik itu di tingkat lokal, regional, hingga internasional. Tak hanya memberikan ancaman multidimensional, besarnya dampak yang diakibatkan oleh pandemi ini juga turut menguji kepemimpinan para pemimpin negara dalam mengendalikan rantai penyebaran COVID-19 secara efektif. Lebih jauh, berbagai pihak pun mengaitkan kesuksesan seorang pemimpin negara melalui kacamata gender. Pasalnya, terdapat stigmatisasi pada karakteristik perempuan yang kerap kali dianggap tidak mumpuni untuk memimpin dan membuat keputusan secara rasional. Dengan menggunakan perspektif feminisme, tulisan ini akan membahas bagaimana karakteristik feminitas dan maskulinitas yang tercermin dalam preferensi kebijakan Kanselir Jerman Angela Merkel, Perdana Menteri Selandia Baru Jacinda Ardern, dan Presiden Taiwan Tsai Ing-wen, secara efektif dapat mengendalikan penyebaran COVID-19 dan pemulihan kondisi multidimensi negara. Keberhasilan ketiga pemimpin perempuan dalam menangani COVID-19 ini menunjukkan adanya perspektif baru dalam kepemimpinan politik yang tidak dapat dibatasi oleh stigma gender terhadap kemampuan perempuan. Kata Kunci: pemimpin perempuan; kepemimpinan politik; pandemi COVID-19; feminisme; kebijakan publik ABSTRACTCOVID-19 is a global problem that is being faced by many countries around the world. Apart from causing an international health crisis, the COVID-19 pandemic also threatens the dynamics of people's lives, be it at the local, regional, and international levels. Not only provide a multi-dimensional threat, but the impact caused by this pandemic also tests the leadership of state leaders in controlling the spread of COVID-19 effectively. Furthermore, various parties try to associate the success of a state leader through a gender perspective. This is due to the stigmatization of women's characteristics who are often considered incompetent to lead and make rational decisions. With a feminist perspective, this paper will discuss how the characteristics of femininity and masculinity reflected in the policy preferences of German Chancellor Angela Merkel, New Zealand Prime Minister Jacinda Ardern, and Taiwanese President Tsai Ing-wen, can effectively control the spread of COVID-19 and multi-dimensional recovery of the country. The success of the three female leaders in dealing with COVID-19 shows a new perspective in political leadership that cannot be limited by gender stigma on women's abilities.

Teaching and Re-Imagining the Role of Medical Sociology in South Africa During COVID-19

2021 ◽  
pp. 175-194
Author(s):  
Chinwe Obuaku-Igwe
Keyword(s):  
South Africa ◽  
South African ◽  
Medical Sociology ◽  
International Health ◽  
Higher Learning ◽  
Virus Family ◽  
Health Crisis ◽  
Face To Face ◽  
Institutions Of Higher Learning

The outbreak of COVID-19 of the SARS-COV virus family took the world by storm beginning February 2020 and became an international health crisis. Due to its unknown origins and manner of transmission, the South African government implemented lockdown measures to curtail the spread of the virus in March. These measures led to the closure of businesses across the country and sectors, including schools. The closure of schools resulted in the migration to online learning for most institutions of higher learning in South Africa. It brings with it challenges, opportunities for innovation, and reimagining pedagogical approaches, particularly in low resource settings. This chapter reflects on the nature and extent of the author's engagement with students enrolled in a health and medical sociology course during the COVID-19 lockdown in South Africa. Here, they reflect on the challenges encountered while moving a course that was designed to be delivered in person (face-to-face) to an online environment.

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