scholarly journals Wide distribution of alternatively coded Lak megaphages in animal microbiomes

2021 ◽  
Author(s):  
Marco Alberto Crisci ◽  
Lin-Xing Chen ◽  
Audra E Devoto ◽  
Adair L Borges ◽  
Nicola Bordin ◽  
...  
Keyword(s):  
Faecal Sample ◽  
Phage Genome ◽  
Wide Distribution ◽  
Western Diet ◽  
Hypothetical Proteins ◽  
Diagnostic Pcr ◽  
Complete Genomes ◽  
Significant Enrichment

Lak phages with alternatively coded ~540 kbp genomes were recently reported to replicate in Prevotella in the gut microbiomes of humans that consume a non-western diet, baboons and some pigs. Here, we investigate the diversity and broader distribution of Lak phages in human and animal microbiomes using diagnostic PCR and genome-resolved metagenomics. Lak phages were detected in 13 different animal types and are particularly prevalent in pigs, with significant enrichment in the hindgut compared to foregut. We reconstructed 34 new Lak genomes, including six curated complete genomes, all of which are alternatively coded. The most deeply branched Lak is from a horse faecal sample and is the largest phage genome from an animal microbiome (~660 kbp). From the Lak genomes, we identified families of hypothetical proteins associated with specific animal types. Overall, we substantially expanded Lak phage diversity and demonstrate their occurrence in a variety of human and animal microbiomes.

scholarly journals Wide Distribution of Phage That Infect Freshwater SAR11 Bacteria

mSystems ◽  
2019 ◽  
Vol 4 (5) ◽  
Author(s):  
Lin-Xing Chen ◽  
Yanlin Zhao ◽  
Katherine D. McMahon ◽  
Jiro F. Mori ◽  
Gerdhard L. Jessen ◽  
...  
Keyword(s):  
Draft Genome ◽  
Wide Distribution ◽  
Freshwater Ecosystems ◽  
Metagenomic Data ◽  
Related Phage ◽  
Lake Mendota ◽  
Worldwide Distribution ◽  
Marine Habitats ◽  
Complete Genomes ◽  
Freshwater Habitats

ABSTRACT Fonsibacter (LD12 subclade) is among the most abundant bacterioplankton in freshwater ecosystems. These bacteria belong to the order Pelagibacterales (SAR11) and are related to Pelagibacter (marine SAR11), which dominates many marine habitats. Although a few Pelagibacter phage (Pelagiphage) have been described, no phage that infect Fonsibacter have been reported. In this study, we describe two groups of Podoviridae phage that infect Fonsibacter. A complete Fonsibacter genome containing a prophage was reconstructed from metagenomic data. A circularized and complete genome related to the prophage, referred to as uv-Fonsiphage-EPL (lysogenic strategy), shows high similarity to marine Pelagiphage HTVC025P. Additionally, we reconstructed three complete genomes and one draft genome of phage related to marine Pelagiphage HTVC010P and predicted a lytic strategy. The similarity in codon usage and cooccurrence patterns of HTVC010P-related phage and Fonsibacter suggested that these phage infect Fonsibacter. Similar phage were detected in Lake Mendota, Wisconsin, where Fonsibacter is also present. A search of related phage revealed the worldwide distribution of some genotypes in freshwater ecosystems, suggesting their substantial role in shaping indigenous microbial assemblages and influence on biogeochemical cycling. However, the uv-Fonsiphage-EPL and one group of HTVC010P-related phage have a more limited distribution in freshwater ecosystems. Overall, the findings provide insights into the genomic features of phage that infect Fonsibacter and expand understanding of the ecology and evolution of these important bacteria. IMPORTANCE Fonsibacter represents a significant microbial group of freshwater ecosystems. Although the genomic and metabolic features of these bacteria have been well studied, no phage infecting them has been reported. In this study, we reconstructed complete genomes of Fonsibacter and infecting phage and revealed their close relatedness to the phage infecting marine SAR11 members. Also, we illustrated that phage that infect Fonsibacter are widely distributed in freshwater habitats. In summary, the results contribute new insights into the ecology and evolution of Fonsibacter and phage.

Immune Electron Microscopy (IEM) of a Canine Adeno-Associated Virus

1974 ◽  
Vol 32 ◽  
pp. 256-257
Author(s):  
Gunter F. Thomas ◽  
M. David Hoggan
Keyword(s):  
Electron Microscopy ◽  
Cell Cultures ◽  
Complement Fixation ◽  
Hepatitis Virus ◽  
Wide Distribution ◽  
Immune Electron Microscopy ◽  
Adeno Associated Virus ◽  
Virus Like Particle ◽  
Dog Kidney ◽  
Green Monkeys

In 1968, Sugimura and Yanagawa described a small 25 nm virus like particle in association with the Matsuda strain of infectious canine hepatitis virus (ICHV). Domoto and Yanagawa showed that this particle was dependent on ICHV for its replication in primary dog kidney cell cultures (PDK) and was resistant to heating at 70°C for 10 min, and concluded that it was a canine adeno-associated virus (CAAV). Later studies by Onuma and Yanagawa compared CAAV with the known human serotypes (AAV 1, 2, 3) and AAV-4, known to be associated with African Green Monkeys. Using the complement fixation (CF) test, they found that CAAV was serologically related to AAV-3 and had wide distribution in the dog population of Japan.

Development of a new modified western diet to induce NASH with obesity and insulin resistance in mice

2015 ◽  
Vol 53 (12) ◽  
Author(s):  
J Henkel ◽  
CD Coleman ◽  
K Jöhrens ◽  
M Kuna ◽  
I Grüner ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Western Diet

RAPID IN VITRO PROPAGATION OF WESTERN GHATS CULTIVAR - COLOCASIA ESCULENTA FOR THE HIGH FREQUENCY OF PLANTLETS

2019 ◽  
Vol 7 (2) ◽  
Author(s):  
Jyothi R ◽  
Srinivasa Murthy K M ◽  
Hossein . ◽  
Veena .
Keyword(s):  
Tissue Culture ◽  
Wide Distribution ◽  
Colocasia Esculenta ◽  
Limiting Factor ◽  
Promising Alternative ◽  
Rapid Multiplication ◽  
Medical Plant ◽  
Total Fat ◽  
Planting Materials

Colocasia esculenta is commonly known as Taro, it is referred to as cocoyam in Nigeria. They are cherished for their rich taste, nutritional and medicinal properties. Every 100 g of taro corms possess 112 Kcal, 26.46 g carbohydrate, 1.50 g protein, 0.20 g total fat and 4.1g fiber (USDA National Nutrient Data Base). Besides its nutritional value, taro is used as a medical plant and provides bioactive compounds used as an anti-cancer drugs. Traditionally, cocoyams are vegetative propagated from tuber fragments, a practice that encourages pathogen distribution. Colocasia esculenta is a widely distributed food crop in the humid tropics and subtropics. Despite of its wide distribution, Taro plants are commonly infected with DsMV and other pathogens. This virus induces conspicuous mosaic, malformation, dwarfing or feathering on leaves in taro. As the results of infection, it reduces the quality and yield of taro production greatly. This virus is thus considered as a major limiting factor in the production of taro. Here plays the importance of  tissue culture plays a major role in producing the disease resistant plants round the year with high quality. For rapid multiplication and production of quality planting materials, tissue culture technology offers promising alternative compared to the traditional production methods. KEYWORDS: Colocasia esculenta, Virus, Pathogens, Conventional propagation, Micropropagation, Yield, Rapid multiplication, Quality

Ecology and biogeography in the introduction to “De bestiis marinis” by Georg Wilhelm Steller

2019 ◽  
Vol 46 (1) ◽  
pp. 63-74
Author(s):  
Stefano Mattioli
Keyword(s):  
Body Size ◽  
Phenotypic Plasticity ◽  
Geographical Distribution ◽  
Functional Traits ◽  
Marine Mammals ◽  
Main Part ◽  
Wide Distribution ◽  
Restricted Range ◽  
Direct Influence ◽  
Modern Biology

The rediscovery of the original, unedited Latin manuscript of Georg Wilhelm Steller's “De bestiis marinis” (“On marine mammals”), first published in 1751, calls for a new translation into English. The main part of the treatise contains detailed descriptions of four marine mammals, but the introduction is devoted to more general issues, including innovative speculation on morphology, ecology and biogeography, anticipating arguments and concepts of modern biology. Steller noted early that climate and food have a direct influence on body size, pelage and functional traits of mammals, potentially affecting reversible changes (phenotypic plasticity). Feeding and other behavioural habits have an impact on the geographical distribution of mammals. Species with a broad diet tend to have a wide distribution, whereas animals with a narrow diet more likely have only a restricted range. According to Steller, both sea and land then still concealed countless animals unknown to science.

Clinical, epidemiological and climatic factors related to the occurrence of cutaneous leishmaniasis in an endemic area in northeastern Brazil

2020 ◽  
Author(s):  
R. S. Oliveira ◽  
K. B. A. Pimentel ◽  
M. L. Moura ◽  
C. F. Aragão ◽  
A. S. Guimarães-e-Silva ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Climatic Factors ◽  
Wide Distribution ◽  
Northeastern Brazil ◽  
Public Health Issue ◽  
Air Humidity ◽  
Disease Occurrence ◽  
Relative Air Humidity ◽  
Age Range ◽  
And Control

Abstract Cutaneous leishmaniasis (CL) is a neglected tropical disease with a wide distribution in the Americas. Brazil is an endemic country and present cases in all states. This study aimed to describe the occurrence, the underlying clinical and epidemiological factors, and the correlation of climatic variables with the frequency of reported CL cases in the municipality of Caxias, state of Maranhão, Brazil. This is a retrospective and descriptive epidemiological study based on data extracted from the Brazilian Information System of Diseases Notification, from 2007 to 2017. Maximum and minimum temperature, precipitation, and relative air humidity data were provided by the Brazilian National Institute of Meteorology. A total of 201 reported autochthonous CL cases were analyzed. The predominance of cases was observed in males (70.1%). The age range between 31 and 60 years old was the most affected, with 96 cases (47.9%). Of the total number of registered cases, 38.8% of the affected individuals were engaged in agriculture-related activities. The georeferenced distribution revealed the heterogeneity of disease occurrence, with cases concentrated in the Western and Southern regions of the municipality. An association was detected between relative air humidity (monthly mean) and the number of CL cases per month (p = 0.04). CL continues to be a concerning public health issue in Caxias. In this context, there is a pressing need to strengthen measures of prevention and control of the disease through the network of health services of the municipality, considering local and regional particularities.

IDENTIFIKASI JENIS JAMUR PATOGEN UNTUK PENGENDALIAN RAYAP TANAH Coptotermes sp.

PERENNIAL ◽  
2010 ◽  
Vol 6 (1) ◽  
pp. 33
Author(s):  
Astuti Arif ◽  
. Syahidah ◽  
Sitti Nuraeni
Keyword(s):  
Key Words ◽  
Pathogenic Fungi ◽  
Wide Distribution ◽  
Subterranean Termite ◽  
The World ◽  
Identification Of Fungi ◽  
Screening And Identification ◽  
Termite Attack ◽  
Penicillium Brevicompactum ◽  
Aspergillus Sp

Fungi have a great diversity and wide distribution at the world. It can be used as the alternative technology for controlling of subterranean termite attack, particularly genus Coptotermes knowned as the wooden destructive organism, by using entomophatogenic fungi. For the purpose of the study, several isolate sources were taken from some locations. The results show that eight numbers of pathogenic fungi was founded by screening and identification of fungi taken from 19 numbers of isolate sources. The pathogenic fungi were Beauveria sp., Penicillium brevicompactum, P. rubrum, Paecilomyces fulvus, Fusarium verticolloides, Pythium sp., and Aspergillus sp. Key words: Jamur entomopatogen, Coptotermes sp.

0204 Effect of the total Western diet via direct or ancestral exposure on estrous cycling in third-generation offspring in mice

2016 ◽  
Vol 94 (suppl_5) ◽  
pp. 97-97
Author(s):  
K. Contreras ◽  
J. Cuthbert ◽  
S. Phatak ◽  
D. Larson ◽  
A. Benninghoff
Keyword(s):  
Western Diet ◽  
Third Generation ◽  
Estrous Cycling ◽  
Generation Offspring
Sign in / Sign up

Export Citation Format

Share Document