scholarly journals Study of growth characteristics of callus, suspension and root crops Scutellaria baicalensis

2021 ◽  
Author(s):  
Anastasia Igorevna Dmitrieva ◽  
Anastasia Michailovna Fedorova ◽  
Violetta Mironovna Le
Keyword(s):  
Growth Index ◽  
Root Culture ◽  
Biologically Active ◽  
Scutellaria Baicalensis ◽  
Root Crops ◽  
Culture Growth ◽  
Nuclear Factor Kb ◽  
Active Substances ◽  
Intensive Growth

Scutellaria baicalensis is a popular traditional plant in Chinese medicine. Widely distributed biologically active substances of Scutellaria baicalensis are flavonoids (baicalin, baicalein, vogonin and vogonoside), which are responsible for the antitumor activity. Their antitumor effect is due to the absorption of oxidative radicals, the weakening of the activity of NF-kB (nuclear factor-kB), the suppression of the expression of the COX-2 gene and the regulation of the cell cycle. In addition, baicalein, baicalin, and vogonin showed strong antioxidant activity. The root culture in vitro of the medicinal plant Scutellaria baicalensis is characterized by intensive growth. The growth index at the end of the cultivation cycle was 40. The growth curve has a standard S-shape, with pronounced growth phases. The stationary phase was observed from 5-7 weeks of cultivation. The root culture growth index was 22.

scholarly journals Analysis of individual biologically active compounds from root crop extracts of purple alfalfa (medicago sativa) in vitro

2021 ◽  
Vol 7 (2) ◽  
pp. 564-574
Author(s):  
Irina Sergeevna Milentyeva ◽  
Anastasiya Igorevna Dmitrieva ◽  
Lyudmila Konstantinovna Asyakina ◽  
Yulia Vladimirovna Golubtsova
Keyword(s):  
Medicago Sativa ◽  
Growth Index ◽  
Root Culture ◽  
Growth Cycle ◽  
Biologically Active ◽  
Root Cultures ◽  
Degree Of Extraction ◽  
Degree Of Purification ◽  
Active Substances

The authors selected the parameters for obtaining root cultures in vitro Medicago sativa. The number of cell culture lines studied is 15. The duration of the growth cycle is less than 50 days. The number of processed explants is 75. The growth characteristics of root cultures of in vitro purple alfalfa (Medicago sativa) were studied. The growth index for the dry biomass of the in vitro root culture of alfalfa was 31. A qualitative and quantitative analysis of the composition of BAS in the biomass of the obtained root cultures of in vitro alfalfa (Medicago sativa) was carried out. Unique biologically active substances (not previously described for alfalfa) were identified.): apigenin, naringenin, myricetin, chrysoeriol, coumestrol, and scopoletin. Rational parameters of isolation of individual BAS from the extract of root cultures of in vitro alfalfa (Medicago sativa) were selected. The degree of extraction of individual BAS is 80%. Methods of purification of individual BAS Medicago sativa have been developed. The degree of purification of individual BAS is 95%.

scholarly journals Perspective processing of turnip root (Brassica rapa) as a source of sulforafan

2020 ◽  
Vol 164 ◽  
pp. 06028 ◽  
Author(s):  
Tatyana Belyakova ◽  
Olga Morozova ◽  
Ekaterina Antonceva ◽  
Lyudmila Zabodalova
Keyword(s):  
Brassica Rapa ◽  
Spectrophotometric Study ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Herbaceous Plant ◽  
Economic Point ◽  
Root Crop ◽  
Root Crops ◽  
Active Substances

Turnip (latin Brassica rapa) is a herbaceous plant from the genus Cruciferous. The root crop is a traditional product that has been producing in Russia since ancient times. This unpretentious herbaceous plant contains many different nutrients. The composition of turnips determines its value as a dietary product. From an economic point of view, the root crop, along with broccoli, can act as a source of biologically active substances like sulforaphane. Processing root crops using extraction will expand the scope of its application. The work presents a laboratory way for producing an extract of sulforaphane, followed by its quantitative determination from turnip varieties Petrovskaya-1. It was shown that the content of sulforaphane turnip extract (0.83 ± 0.03) mg in 1 cm3 extract. Next, the antioxidant ability of the extract was determined. A spectrophotometric study of antioxidant activity using a model adrenaline autooxidation reaction (in vitro) revealed that the aqueous extract has a pronounced antioxidant effect. It was registered that at an exposure time of 5 min, the AOA of the extract was 49.0%, at 10 min it was 13.0%. We can say that the processing of root crops is promising as a source of biologically active substances.

Optimization of the parameters of extraction of root cultures

2021 ◽  
pp. 82-85
Author(s):  
Людмила Константиновна Асякина ◽  
Анастасия Михайловна Федорова ◽  
Любовь Сергеевна Дышлюк
Keyword(s):  
Maximum Yield ◽  
Federal District ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Scutellaria Baicalensis ◽  
Extraction Temperature ◽  
Root Cultures ◽  
Optimal Parameters ◽  
Active Substances

Настоящая работа затрагивает тему оптимизации процесса экстракции биологически активных веществ из биомассы корневых культур in vitro лекарственных растений Сибирского федерального округа, а именно шлемника байкальского, шлемника обыкновенного и лапчатки белой. Параметры, которые влияли на ход процесса, были выбраны следующие: продолжительность процесса, температура экстракции и гидромодуль. В ходе эксперимента выяснилось, что для максимального выхода экстракта шлемника байкальского необходимо соблюдать следующие параметры: соотношение объема растворителя, наиболее эффективного в данном случае 70 %-ного этанола, к массе исходного сырья 1:10, продолжительность процесса 60 мин, температура 50 °С. Максимальный выход биологически активных веществ из шлемника обыкновенного будет достигаться при температуре экстракции 60 °С в течение 60 мин с использованием 70 %-ного этанола в качестве органического растворителя в соотношении к высушенному образцу 1:10. Оптимальные параметры извлечения активных веществ из лапчатки белой: соотношение экстрагента, в качестве которого выступает диэтиловый эфир, к исходному сырью 1:20, продолжительность 60 мин при температуре экстракции 40 °С. Извлечение экстрактов из культур in vitro позволит сохранить популяцию растений, произрастающих в естественных условиях. This work touches on the topic of optimization of the process of extraction of biologically active substances from the biomass of root cultures in vitro of medicinal plants of the Siberian Federal District, namely Scutellaria galericulata, Scutellaria Baicalensis, Potentilla alba . The parameters that influenced the course of the process were chosen as follows: process duration, extraction temperature and hydronic module. In the course of the experiment, it turned out that for the maximum yield of the Scutellaria Baicalensis extract, the following parameters must be observed: the ratio of the volume of the solvent, the most effective in this case 70% ethanol, to the weight of the feedstock 1:10, the duration of the process is 60 min, the temperature is 50 °C. The maximum yield of biologically active substances from Scutellaria galericulata will be achieved at an extraction temperature of 60 °C for 60 minutes using 70 % ethanol as an organic solvent in a ratio of 1:10 to the dried sample. The optimal parameters for the extraction of active substances from Potentilla alba are: the ratio of the extractant, which is diethyl ether, to the feedstock 1:20, lasting 60 minutes at an extraction temperature of 40 °C. The extraction of extracts from in vitro cultures will allow preserving the population of plants growing in natural conditions.

scholarly journals Determination of the Qualitative Composition of Biologically Active Substances of Extracts of In Vitro Callus, Cell Suspension, and Root Cultures of the Medicinal Plant Rhaponticum carthamoides

2021 ◽  
Vol 11 (6) ◽  
pp. 2555
Author(s):  
Lyudmila Asyakina ◽  
Svetlana Ivanova ◽  
Alexander Prosekov ◽  
Lyubov Dyshlyuk ◽  
Evgeny Chupakhin ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Cell Suspension ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Root Cultures ◽  
Qualitative Composition ◽  
Callus Cell ◽  
Rhaponticum Carthamoides ◽  
Active Substances

This work aims to study the qualitative composition of biologically active substance (BAS) extracts in vitro callus, cell suspension, and root cultures of the medicinal plant Rhaponticum carthamoides. The research methodology is based on high-performance liquid chromatography, and 1H nuclear magnetic resonance (NMR) spectra, to study the qualitative and quantitative analysis of BAS. The results of the qualitative composition analysis of the dried biomass extracts of in vitro callus, cell suspension and root cultures showed that the main biologically active substances in the medicinal plant Rhaponticum carthamoides are 2-deoxy-5,20,26-trihydroxyecdyson (7 mg, yield 0.12%), 5,20,26-trihydroxyecdyson 20,22-acetonide (15 mg, yield 0.25%), 2-deoxy-5,20,26-trihydroxyecdyson 20,22-acetonide (6 mg, yield 0.10%), 20,26-dihydroxyecdyson 20,22-acetonidecdyson 20,22-acetonide (5 mg, yield 0.09%), and ecdyson 20,22-acetonide (6 mg, yield 0.10%). In the future, it is planned to study the antimicrobial, antioxidant, and antitumor activity of BAS of extracts of in vitro callus, cell suspension, and root cultures of the medicinal plant Rhaponticum carthamoides, for the production of pharmaceuticals and dietary supplements with antitumor, antimicrobial and antioxidant effects.

scholarly journals Comparative investigation of sesquiterpene components of essential oils originating from intact plants and hairy root chamomile cultures

2021 ◽  
Vol 6 (2) ◽  
pp. 028-049
Author(s):  
Éva Szőke ◽  
Éva Lemberkovics
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Hairy Root ◽  
Folk Medicine ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Comparative Investigation ◽  
Biologically Active

The importance of chamomile (Chamomilla recutita) inflorescence is widely known in classical and folk medicine, with the largest group of its effective constituents forming the essential oil (chamazulene, a-bisabolol, α-farnesene, trans-β-farnesene, spathulenol, cis/trans-en-in-dicycloethers). Among cultivated species, the Hungarian BK-2 contains more chamazulene in its essential oil than the German Degumil type, which is mainly cultivated for its a-bisabolol. Both components have important antiinflammatory activities. Wild populations can be easily distinguished from cultivated ones by their high amount of bisaboloides, particularly the flower of Hungarian Szabadkígyós wild type, which contained on average 48 % of the biologically active (-)-a-bisabolol. The population of Szabadkígyós has good salt tolerance which is important owing to global warming, because the proportion of saline areas is increasing worldwide. To keep the genome of Szabadkígyós having high (-)-a-bisabolol content, Szőke and research team used biotechnological methods. Sterile plantlets, were infected by Agrobacterium rhizogenes strains #A-4, #15834, #R-1601. The hairy root clones possessing the best growing and biosynthetical potential were multiplied for phytochemical investigations. Pharmacologically important compounds of their essential oils were followed in great detail. The amount of in vitro cultured terpenoids and polyin compounds was compared with that of in vivo plants. GC-MS studies showed that sterile chamomile cultures generated the most important terpenoid and polyin compounds characteristics of the mother plant. Berkheyaradulene, geranyl-isovalerat and cedrol as new components were identified in these sterile cultures. The main component of hairy root cultures (D/400, D/1, D/100 and Sz/400) was tr-b-farnesene and in addition one new compound: a-selinene was identified. Hairy root culture originated from chamomile collected in Szabadkígyós was intensive increased the essential oil content and pharmacological active compounds: (-) -α-bisabolol and β-eudesmol was also synthetized in large quantity. Furthermore, in vitro organized cultures were made from this population to obtain propagation material containing numerous active substances.

scholarly journals Evaluation of in vitro immunomodulatory properties of biologically active substances and drugs

2019 ◽  
Vol 16 (2) ◽  
pp. 234-243
Author(s):  
A. V. Duzh ◽  
A. Y. Hancharou
Keyword(s):  
Biologically Active ◽  
Interleukin 12 ◽  
Oxygen Species ◽  
Combined Method ◽  
Hla Dr ◽  
Immunomodulatory Properties ◽  
Factor Α ◽  
Active Substances ◽  
Necrosis Factor

A new combined method for screening of immunomodulatory properties of drugs was developed, including: expression of CD80, HLA-DR, CD32, CD205 and CD197, interleukin-12 production and apoptosis by DCs; production of reactive oxygen species and evaluation of phagocytosis by neutrophils; expression of CD69 and production of tumor necrosis factor-α by Jurkat-tat cells; and CD80 and HLA-DR expression, viability and apoptosis using the Daudi cell line.

scholarly journals Biotechnological aspects of obtaining biologically active substances of Adonis vernalis

2021 ◽  
Vol 4 (1) ◽  
pp. 126-130
Author(s):  
V. R. Hamada ◽  
Keyword(s):  
Antioxidant Activity ◽  
Biological Activity ◽  
Raw Materials ◽  
Callus Cultures ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
In Vitro Cultivation ◽  
Plant Raw Materials ◽  
Active Substances

This article shows the possibility of using a biotechnological method to obtain biologically active compounds based on in vitro cultivation of callus cultures. The callus biomass of Adonis vernalis was obtained in vitro by using the biotechnological method. The extracts based on callus biomass of Adonis vernalis were obtained. The content of flavonoids and phenolic compounds was determined. The antioxidant activity of callus biomass of Adonis vernalis has also been studied. The research results show that the content of biologically active substances and biological activity in callus biomass does not differ from plant raw materials.

scholarly journals Usability of vegetable extracts in the protection against Alternaria alternata

2012 ◽  
pp. 113-116
Author(s):  
Csaba Tamás Tóth ◽  
Mária Csubák
Keyword(s):  
Biological Activity ◽  
Alternaria Alternata ◽  
Petri Dish ◽  
Effective Concentration ◽  
Ambrosia Artemisiifolia ◽  
Point Of View ◽  
Biologically Active ◽  
Minimum Effective Concentration ◽  
Active Substances

In our country, wormwood ragweed (Ambrosia artemisiifolia) may cause serious problems. Nearly 5 million hectares of agricultural area was infected with ragweed (Ambrosia artemisiifolia), which is believed useless weed. Allergological point of view, most problematic weeds adventive. However, many physiologically very beneficial compound also included, those with the effects have been known also by the Indians. On this basis, herbs can be thought of as ragweed. Our goal was to present that the ragweed contains antifungal active substances as well. In this paper we tested the biological activity of the extracts against Alternaria alternata F.00750 in vitro. We related based on our examination that ragweed contains biologically active agents, by which it is hampered the reproduction of the Alternaria alternata. The minimum effective concentration was 300 mg extract in a Petri dish, which was three days inhibited the growth of fungus. Full fungicidal effect was observed over dose 525 mg.

Studies on the generation of biologically active substances by Trypanosoma theileri in vitro

1980 ◽  
Vol 28 (2) ◽  
pp. 178-184 ◽  
Author(s):  
I.R. Tizard ◽  
J. Gorski ◽  
J. Sheppard ◽  
A. Mellors ◽  
P. Hambrey
Keyword(s):  
Biologically Active Substances ◽  
Biologically Active ◽  
Active Substances ◽  
Trypanosoma Theileri
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