scholarly journals Direct multiplex recombinase polymerase amplification for rapid detection of Staphylococcus aureus and Pseudomonas aeruginosa in food

2021 ◽  
Author(s):  
Hong Diem Tran ◽  
Thi Hau Tran ◽  
Nguyen Minh Trang Pham ◽  
Huong Thi Thu Phung
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Foodborne Pathogens ◽  
Incubation Temperature ◽  
High Sensitivity ◽  
Recombinase Polymerase Amplification ◽  
Food And Beverage ◽  
Specialized Equipment ◽  
Efficient Alternative ◽  
Limit Detection

Foodborne illness undermines human health by causing fever, stomachache and even lethality. Among foodborne bacterial pathogens, Staphylococcus aureus and Pseudomonas aeruginosa are of extraordinary significance which drive reasons of food and beverage poisoning in numerous cases. Today, PCR has been widely used to examine the presence of different foodborne pathogens. However, PCR requires specialized equipment and skillful personnel which limit its application in the field. Recently, there is an emerging of isothermal PCR methods in which the reactions occur at low and constant temperatures, allowing their application in restricted-resource settings. In this work, multiplex Recombinase Polymerase Amplification (RPA) was used to simultaneously detect S. aureus and P. aeruginosa with high sensitivity and specificity. The limit detection of multiplex RPA was 10 and 30 fg/reaction of genomic DNAs of S. aureus and P. aeruginosa, respectively. Besides, the reaction time was reduced to only 25 minutes with a low incubation temperature of 39 °C. Markedly, multiplex RPA reactions succeeded to directly detect as low as 1 and 5 CFU/reaction of S. aureus and P. aeruginosa cells, respectively without the requirement of extracting DNA genome. Moreover, the multiplex RPA reliably detected the two foodborne bacteria in milk, fruit juice and bottled water samples. In general, the direct multiplex RPA described in this study is a rapid, simple, sensitive and efficient alternative tool that could be used to detect the presence of S. aureus and P. aeruginosa without the necessity of costly devices and high-trained staff.

scholarly journals Multiplex Recombinase Polymerase Amplification Assay for the Simultaneous Detection of Three Foodborne Pathogens in Seafood

Foods ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 278 ◽  
Author(s):  
Biao Ma ◽  
Jiali Li ◽  
Kai Chen ◽  
Xiaoping Yu ◽  
Chuanxin Sun ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Foodborne Pathogens ◽  
Vibrio Parahaemolyticus ◽  
Salmonella Enteritidis ◽  
Simultaneous Detection ◽  
Test Strip ◽  
Food Samples ◽  
Recombinase Polymerase Amplification ◽  
Quantitative Detection ◽  
Test Strip Reader

Foodborne pathogens can cause foodborne illness. In reality, one food sample may carry more than one pathogen. A rapid, sensitive, and multiple target method for bacteria detection is crucial in food safety. For the simultaneous detection of Staphylococcus aureus, Vibrio parahaemolyticus, and Salmonella Enteritidis, multi-objective recombinase polymerase amplification (RPA) combined with a lateral flow dipstick (LFD) was developed in this study. The whole process, including amplification and reading, can be completed in 15 min at 37 °C. The detection limits were 2.6 × 101 CFU/mL for Staphylococcus aureus, 7.6 × 101 CFU/mL for Vibrio parahaemolyticus, and 1.29 × 101 CFU/mL for Salmonella Enteritidis. Moreover, colored signal intensities on test lines were measured by a test strip reader to achieve quantitative detection for Staphylococcus aureus (R2 = 0.9903), Vibrio parahaemolyticus (R2 = 0.9928), and Salmonella Enteritidis (R2 = 0.9945). In addition, the method demonstrated good recoveries (92.00%–107.95%) in the testing of spiked food samples. Therefore, the multiplex LFD-RPA assay is a feasible method for the rapid, sensitive, and quantitative detection of bacterial pathogens in seafood.

scholarly journals Antibiogram of the Common Uropathogenic Bacteria among Yemeni Patients in Sana’a City: A Recent Report

2018 ◽  
pp. 1-10 ◽  
Author(s):  
Hafez Alsumairy ◽  
Tawfique K. AlZubiery ◽  
Talal Alharazi ◽  
Mufeed Baddah ◽  
Adel Al-Zubeiry
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Empirical Therapy ◽  
High Sensitivity ◽  
Resistance Rate ◽  
Beta Lactam ◽  
Cross Sectional ◽  
Sensitivity Pattern ◽  
Uropathogenic Bacteria

Aims: This study aimed to identify the prevalence and antimicrobial susceptibility of the commonly isolated uropathogens in Sana’a city, Yemen. Study Design:  A cross-sectional and descriptive study. Place and Duration of Study: The study was carried out at the hospitals and clinics of Sana'a city, Yemen between October 2016 and March 2017. Methodology: Clean-catch mid-stream urine samples were collected to detect the most common uropathogenic bacteria and their antibiotic susceptibility using Kirby Bauer standardized method. Results: Urine cultures yielded 170 significant bacterial growths of uropathogens. Escherichia coli was the most often isolated pathogen (43.5%), followed by Klebsiella pneumoniae (24.7%), Pseudomonas aeruginosa (20.0%) and Staphylococcus aureus (11.8%). The overall sensitivity was high to an excellent pattern for Carbapenems, Nitrofurantoin, Amikacin, and Piperacillin-Tazobactam. Escherichia coli shows an excellent sensitivity (88%) for Nitrofurantoin and Imipenem, followed by (85%) Ertapenem. Pseudomonas aeruginosa exhibited moderate resistance to Carbapenems, Moxifloxacin, and Piperacillin-Tazobactam in this study. Staphylococcus aureus was more vulnerable to all Quinolones except Nalidixic acid and it displays a high sensitivity pattern, 90% for both Nitrofurantoin and Gentamicin, 83% for Penicillin, 80% for both Minocycline. Antibiogram of isolated organisms revealed that there was resistance to two and more antimicrobials. Conclusion: In this study, we observe a high resistance rates to Beta-lactam, Quinolones, and Macrolides antibiotics. Nevertheless, most uropathogenic isolates were still sensitive to Nitrofurantoin, Imipenem, Ertapenem, and Amikacin, they considered as a proper antibiotics for empirical therapy of UTIs. Establishment of antibiogram of locally isolated organisms is necessary to avoid indiscriminating use of antibiotic and to decrease the resistance rate in our community.

scholarly journals Microbiological study of Otitis externa in Sheep in Diwaniya City

2011 ◽  
Vol 10 (1) ◽  
pp. 32
Author(s):  
S.A. Ali
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Otitis Externa ◽  
Bacterial Infections ◽  
High Sensitivity ◽  
Mannheimia Haemolytica ◽  
Isolation And Identification ◽  
Microbiological Study ◽  
Awassi Sheep ◽  
Sensitivity Tests

In this study, twenty-eight Awassi sheep suffering from otitis externa were examined for isolation and identification of bacterial infections.  The study showed 53.6% of the sheeps were positive for bacterical islates.The results showed that Pseudomonas aeruginosa was the most common bacteria (39,1%) followed by Staphylococcus aureus (26%), Mannheimia haemolytica and Staphylococcus epidermidiis (13%) for each, while E.coli and Streptococcus spp. were less in common (1%) for each. Sensitivity tests of these isolates were studied and show high sensitivity to ciprofloxacin and norfloxacin and highly resistant to Ampicillin.

scholarly journals Microbiological Profile of Infectious Keratitis in a Portuguese Tertiary Centre

2019 ◽  
Vol 2019 ◽  
pp. 1-6 ◽  
Author(s):  
Cláudia Oliveira-Ferreira ◽  
Mariana Leuzinger-Dias ◽  
João Tavares-Ferreira ◽  
Luís Torrão ◽  
F. Falcão-Reis
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibiotic Resistance ◽  
Streptococcus Pneumoniae ◽  
High Sensitivity ◽  
Infectious Keratitis ◽  
Adequate Treatment ◽  
Microbiological Profile ◽  
Tertiary Centre ◽  
Tertiary Center

The microbiological profile of infectious keratitis has shown great differences across the world. Due to the continuous shifting trends in microbiological profile and antibiotic resistance patterns reported in several studies, constant local updates are crucial to provide an adequate treatment. The propose of this study was to analyze the incidence of infectious keratitis, possible changing trends in microbiological profile, and bacteria sensitivity to commonly used antibiotics, in our tertiary center, in the last 10 years. A retrospective study was performed, based on the survey review of electronic medical records of all patients with presumed infectious keratitis, between January 1, 2009, and December 31, 2018. Microbial cultures were performed, and patients were treated according to an internal protocol. A total of 1360 samples were included. We obtained a 35.1% culture-positive rate. Bacteria accounted for 76.78% of all positive scrapes (53.34% were Gram positive and 23.44% were Gram negative), Acanthamoeba for 12.13%, fungi for 8.16%, and virus for 2.93%. The most frequent agent identified was Corynebacterium macginleyi (18.41%), followed by Staphylococcus aureus (17.78%), Streptococcus pneumoniae (9.41%), and Pseudomonas aeruginosa (9.00%). We identified at least one ophthalmologic risk factor in 410 patients (85.77%). Trauma and contact lens wear were the most common risk factors found, accounting for 34.94% (n = 167) and 33.47% (n = 160) of cases. Sensitivity to fluoroquinolones and aminoglycosides was tested in all bacterial isolates, presenting values of 96.66% and 98.12%. In our region, the most common bacteria are Staphylococcus aureus, Streptococcus pneumoniae, and Pseudomonas aeruginosa, and they showed high sensitivity rates to first-line antibiotics, without any modification or emergence of antibiotic resistance trends during the 10 years of the study. For this reason, we decided to maintain the same internal protocol in our tertiary centre.

Case of a diabetic man cured from rhinocerebral zygomycosis

2010 ◽  
Vol 151 (39) ◽  
pp. 1591-1596
Author(s):  
Botond Lakatos ◽  
Radka Nikolova ◽  
László Ocskay ◽  
Judit Csomor ◽  
Gyula Prinz
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Amphotericin B ◽  
Rhizopus Oryzae

A zygomycosis (mucormycosis) ritka, kifejezetten agresszív lefolyású opportunista infekció, amelyet a Zygomycota (Járomspórás gombák) törzs Zygomycetes osztályába tartozó gombák okoznak. Jellemzően immunhiány, neutropenia, vastúlterhelés, hematológiai betegség, illetve diabeteses ketoacidosis eseteiben fordul elő. Esetismertetés: A szerzők 18 éves, 9 éve inzulindependens diabetes mellitusban szenvedő, rossz compliance-szel rendelkező beteg kórtörténetét ismertetik. Súlyos állapotban, beszűkült tudattal, ketoacidosisban, orrmelléküreg-gyulladásra utaló egyoldali periorbitalis duzzanattal, bőrpírral került kórházba. Bár az endoszkópos sinusműtét során vett váladékból methicillinrezisztens Staphylococcus aureus és Pseudomonas aeruginosa tenyészett, a beteg anamnézise, klinikuma zygomycosis gyanúját keltette, emiatt amphotericin-B-kezelést indítottak. Szövettani, illetve mikrobiológiai vizsgálatok során Rhizopus oryzae okozta invazív infekció igazolódott. Megbeszélés: Az antifungális kezelés ismételt sebészeti beavatkozásokkal és adjuváns granulocytakolónia-stimuláló faktor alkalmazásával kiegészítve eredményesnek bizonyult. A beteg négy hónappal hazabocsátását követően él és panaszmentes. Orv. Hetil., 2010, 39, 1591–1596.

Antibacterial Activity of Desert Truffles from Saudi Arabia Against Staphylococcus aureus and Pseudomonas aeruginosa

2017 ◽  
Vol 19 (2) ◽  
pp. 121-125 ◽  
Author(s):  
Domenico Schillaci ◽  
Maria Grazia Cusimano ◽  
Stella Maria Cascioferro ◽  
Vita Di Stefano ◽  
Vincenzo Arizza ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Saudi Arabia ◽  
Desert Truffles

The Study of Bacillus Subtils Antimicrobial Activity on Some of the Pathological Isolates

2019 ◽  
Vol 9 (02) ◽  
Author(s):  
Hussein A Kadhum ◽  
Thualfakar H Hasan2
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Bacterial Growth ◽  
Broad Spectrum ◽  
Inhibitory Activity ◽  
Bacterial Pathogens ◽  
Inhibitory Ability ◽  
Selection Of

The study involved the selection of two isolates from Bacillus subtilis to investigate their inhibitory activity against some bacterial pathogens. B sub-bacteria were found to have a broad spectrum against test bacteria such as Staphylococcus aureus and Pseudomonas aeruginosa. They were about 23-30 mm and less against Klebsiella sp. The sensitivity of some antibodies was tested on the test samples. The results showed that the inhibitory ability of bacterial growth in the test samples using B. subtilis extract was more effective than the antibiotics used.

ATIVIDADE ANTIMICROBIANA IN VITRO DO EXTRATO AQUOSO, HIDROALCOÓLICO E ALCOÓLICO DE FOLHAS DE ESPÉCIES DA FAMÍLIA LAMIACEAE

2018 ◽  
Vol 4 ◽  
Author(s):  
Karlynne Freire Mendonça ◽  
José Klauber Roger Carneiro ◽  
Maria Auxiliadora Silva Oliveira
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Enterococcus Faecalis ◽  
Streptococcus Pyogenes ◽  
Mentha Arvensis ◽  
Ocimum Gratissimum ◽  
Mueller Hinton ◽  
Plectranthus Amboinicus

Objetivos: avaliar a atividade antimicrobiana em extrato aquoso, hidroalcoólico e alcoólico das folhas de espécies da família Lamiaceae frente a bactérias de interesse. Método: Foram escolhidas quatro espécies: Ocimum gratissimum, Plectranthus amboinicus, Mentha arvensis e Plectranthus barbatus. A partir das folhas foram confeccionados os extratos aquoso, hidroalcoólico e alcoólico nas concentrações 100mg/mL, 50mg/mL e 25mg/mL. Foram selecionadas as bactérias Streptococcus pyogenes, Enterococcus faecalis, Staphylococcus aureus e Pseudomonas aeruginosa para os ensaios de antibiose em Ágar Mueller-Hinton. Resultados: P. barbatus, em seu extrato hidroalcoólico mostrou ativo nas três concentrações para bactéria S. aureus, e ainda foi ativo para P. aeruginosa, demonstrando no extrato alcoólico atividade frente as bactérias. Para M. arvensis e P. amboinicus, seus extratos hidroalcoólico e alcoólico apresentaram atividade para S. aureus. Conclusão: Sugere-se que as espécies em questão apresentem boa atividade antimicrobiana, sendo necessária a realização de mais estudos para melhor entender esse mecanismo.

Activité antibactérienne de l’huile essentielle de Pelargonium x asperum et son potentiel synergique avec la nisine

2019 ◽  
Vol 17 (3) ◽  
pp. 140-148 ◽  
Author(s):  
A. Ouelhadj ◽  
L. Ait Salem ◽  
D. Djenane
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Nous Avons ◽  
Activité Antibactérienne

Ce travail vise l’étude de l’activité antibactérienne de l’huile essentielle (HE) de Pelargoniumx asperum et de la bactériocine, la nisine seul et en combinaison vis-à-vis de six bactéries dont quatre sont multirésistantes d’origine clinique. L’activité antibactérienne in vitro a été évaluée par la méthode de diffusion sur gélose. La concentration minimale inhibitrice (CMI) est aussi déterminée pour HE. Les résultats ont révélé une activité antibactérienne significative exercée par HE visà-vis de Staphylococcus aureus (ATCC 43300), Staphylococcus aureus et Escherichia coli avec des diamètres d’inhibition de 36,00 ; 22,50 et 40,00 mm, respectivement. Cependant, l’HE de Pelargonium asperum a montré une activité antibactérienne supérieure par rapport à la nisine. Les valeurs des CMI rapportées dans cette étude sont comprises entre 1,98–3,96 μl/ml. Les combinaisons réalisées entre HE et la nisine ont montré un effet additif vis-à-vis de Escherichia coli (ATCC 25922) avec (50 % HE Pelargonium asperum + 50 % nisine). Par contre, nous avons enregistré une synergie vis-à-vis de Klebsiella pneumoniae avec (75 % HE Pelargonium asperum + 25 % nisine) et contre Pseudomonas aeruginosa avec les trois combinaisons testées. Les résultats obtenus permettent de dire que l’HE de Pelargonium asperum possède une activité antibactérienne ainsi que sa combinaison avec la nisine pourrait représenter une bonne alternative pour la lutte contre l’antibiorésistance.

Chemical Composition and Antibacterial Activity of Essential Oils of Three Medicinal Plants from Algerian Semi-Arid Climatic Zone

2018 ◽  
Vol 16 (S1) ◽  
pp. S155-S163 ◽  
Author(s):  
S. Mehalaine ◽  
O. Belfadel ◽  
T. Menasria ◽  
A. Messaili
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Rosmarinus Officinalis ◽  
Salvia Officinalis ◽  
Thymus Algeriensis

The present study was carried out to determine, for the first time, the chemical composition and antibacterial activity of essential oils derived from the aerial parts of three aromatic plants Thymus algeriensis Boiss & Reut, Rosmarinus officinalis L., and Salvia officinalis L. growing under semiarid conditions. The essential oils were chemically analyzed and identified by gas chromatography (GC) and GC/ mass spectrometry (GC/MS) and their antimicrobial activity was individually evaluated against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa using both agar disk diffusion and agar dilution methods. The major constituents of Thymus algeriensis essential oil were identified as camphor (13.62%), 1,8-cineol (6.00%), borneol (5.74%), viridiflorol (4.00%), and linalool (3.93%). For Rosmarinus officinalis essential oil, 48 compounds were characterized, of which the main constituents were camphor (17.09%), Z-β-ocimene (10.88%), isoborneol (9.68%), α-bisabolol (7.89%), and borneol (5.11%). While, Salvia officinalis essential oil was characterized by β-thujone (16.44%), followed by viridiflorol (10.93%), camphor (8.99%), 1,8-cineol (8.11%), trans-caryophyllene (5.85%), and α-humulene (4.69%) as the major components. Notably, results from antibacterial screening indicated that Thymus algeriensis and Salvia officinalis essential oils exhibited a strong inhibitory effect against both Escherichia coli and Staphylococcus aureus compared to Rosmarinus officinalis essential oil. Further, less activity was recorded against Pseudomonas aeruginosa for the three tested essential oils.

Sign in / Sign up

Export Citation Format

Share Document