scholarly journals Microbiological study of Otitis externa in Sheep in Diwaniya City

2011 ◽  
Vol 10 (1) ◽  
pp. 32
Author(s):  
S.A. Ali
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Otitis Externa ◽  
Bacterial Infections ◽  
High Sensitivity ◽  
Mannheimia Haemolytica ◽  
Isolation And Identification ◽  
Microbiological Study ◽  
Awassi Sheep ◽  
Sensitivity Tests

In this study, twenty-eight Awassi sheep suffering from otitis externa were examined for isolation and identification of bacterial infections.  The study showed 53.6% of the sheeps were positive for bacterical islates.The results showed that Pseudomonas aeruginosa was the most common bacteria (39,1%) followed by Staphylococcus aureus (26%), Mannheimia haemolytica and Staphylococcus epidermidiis (13%) for each, while E.coli and Streptococcus spp. were less in common (1%) for each. Sensitivity tests of these isolates were studied and show high sensitivity to ciprofloxacin and norfloxacin and highly resistant to Ampicillin.

scholarly journals Pseudomonas aeruginosa and Staphylococcus aureus virulence factors as biomarkers of infection

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Artur J. Sabat ◽  
Daniele Pantano ◽  
Viktoria Akkerboom ◽  
Erik Bathoorn ◽  
Alexander W. Friedrich
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacterial Infections ◽  
Clinical Status ◽  
Clinical Samples ◽  
Pathogenic Microorganisms ◽  
Bacterial Identification ◽  
Accurate Identification ◽  
Culture Independent ◽  
And Staphylococcus Aureus

Abstract The gold standard for the diagnosis of bacterial infections in clinical samples is based on culture tests that are time-consuming and labor-intense. For these reasons, an extraordinary effort has been made to identify biomarkers as the tools for sensitive, rapid and accurate identification of pathogenic microorganisms. Moreover, biomarkers have been tested to distinguish colonization from infection, monitor disease progression, determine the clinical status of patients or predict clinical outcomes. This mini-review describes Pseudomonas aeruginosa and Staphylococcus aureus biomarkers, which contribute to pathogenesis and have been used in culture-independent bacterial identification directly from patient samples.

scholarly journals Optimization of a High-Throughput 384-Well Plate-Based Screening Platform with Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 15442 Biofilms

2020 ◽  
Vol 21 (9) ◽  
pp. 3034 ◽  
Author(s):  
Shella Gilbert-Girard ◽  
Kirsi Savijoki ◽  
Jari Yli-Kauhaluoma ◽  
Adyary Fallarero
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
High Throughput ◽  
High Throughput Screening ◽  
Bacterial Infections ◽  
Rapid Screening ◽  
Bacterial Biofilms ◽  
Main Concern ◽  
Assay Optimization ◽  
Screening Platform

In recent years, bacterial infections have become a main concern following the spread of antimicrobial resistance. In addition, bacterial biofilms are known for their high tolerance to antimicrobials and they are regarded as a main cause of recalcitrant infections in humans. Many efforts have been deployed in order to find new antibacterial therapeutic options and the high-throughput screening (HTS) of large libraries of compounds is one of the utilized strategies. However, HTS efforts for anti-biofilm discovery remain uncommon. Here, we miniaturized a 96-well plate (96WP) screening platform, into a 384-well plate (384WP) format, based on a sequential viability and biomass measurements for the assessment of anti-biofilm activity. During the assay optimization process, different parameters were evaluated while using Staphylococcus aureus and Pseudomonas aeruginosa as the bacterial models. We compared the performance of the optimized 384WP platform to our previously established 96WP-based platform by carrying out a pilot screening of 100 compounds, followed by the screening of a library of 2000 compounds to identify new repurposed anti-biofilm agents. Our results show that the optimized 384WP platform is well-suited for screening purposes, allowing for the rapid screening of a higher number of compounds in a run in a reliable manner.

scholarly journals Multicentre clinical trial to evaluate microbial spectrum in acute diffuse otitis externa in the residents of Ukraine

2021 ◽  
pp. 55-61
Author(s):  
Vasyl I. Popovych
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Otitis Externa ◽  
Healthy Population ◽  
External Ear ◽  
Ear Canal ◽  
Microbial Composition ◽  
Gram Negative ◽  
External Ear Canal ◽  
Microbial Spectrum

Introduction: Acute diffuse otitis externa is a spread skin inflammation of the external ear canal of the bacterial origin. It is reported at an incidence of up to 10% among healthy population of all age groups. Treatment is known to be based on the empirical approach for prescribing topical antibacterials. Therefore studying the bacterial spectrum of otitis externa causative agents is of great value in terms of relevant antimicrobial therapy. Aim: The objective of the study was to evaluate microbial spectrum in diffuse otitis externa in patients – residents of Ukraine. Materials and methods: Four hundred and ninety-three out-patients diagnosed with acute diffuse otitis externa were enrolled. Microbial composition of the external ear canal microflora was tested and aetiologically relevant titres of colony-forming units (CFU) were determined. Criteria for evaluation: titres of 104 to 106 CFU and >106 CFU were considered aetiologically relevant. titres of <104 CFU were considered aetiologically nonrelevant. Results and discussion: In the majority of cases of acute diffuse otitis externa, infection with Staphylococcus aureus in aetiologically relevant titres is reported in 53.2% and Pseudomonas aeruginosa in 23.6%. Cases of multimicrobial infection predominantly involving Gram-negative flora were also reported in 27.9%; each of them results in no more than 2 to 3% of otitis externa. Titres of Candida spp. (3.2%) and Staphylococcus epidermidis (2.6%) were measured as aetiologically non-relevant. Conclusion: common microbial pathogens in acute diffuse otitis externa in patients of the Ukrainian population are Staphylococcus aureus and Pseudomonas aeruginosa. Multimicrobial associations predominantly involving Gram-negative flora, which do not exceed 2 to 3% of cases. Fungal infections are a rare cause of otitis externa.

scholarly journals Monitoring of bacterial agents isolated from small cattle on the territory of certain regions of the Russian Federation

2021 ◽  
Author(s):  
P.N. Shastin ◽  
A.V. Kapustin ◽  
E.A. Yakimova ◽  
E.V. Ivanov ◽  
A.I. Laishevtsev
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Clostridium Perfringens ◽  
Russian Federation ◽  
Pasteurella Multocida ◽  
Mannheimia Haemolytica ◽  
Sheep Breeding ◽  
Trueperella Pyogenes ◽  
The Russian Federation ◽  
Bibersteinia Trehalosi

The paper presents the results of bacterial screening of goat and sheep breeding enterprises in certain regions of Russia (Tver, Moscow, Smolensk regions, as well as the Republic of Mari-El and Tatarstan), conducted in the period from 2018 to 2021. In the course of this work, 556 samples of sectional material (heart, lungs, gastrointestinal tract, spleen, kidneys, liver, lymph nodes, breast, flushes from the genitourinary system, as well as exudate from purulent lesions) were subjected to a comprehensive bacteriological study. As a result of the conducted studies, 1223 isolates belonging to 25 families (111 bacterial species) were isolated and identified (by the method of time-of-flight mass spectrometry MALDI-ToF). According to the data obtained, the incidence of Escherichia coli isolation was 10.95%, Trueperella pyogenes – 5.47%, Staphylococcus aureus – 5.31%, Proteus mirabilis – 4.08%, Mannheimia haemolytica – 4.00%, Enterococcus faecalis – 3.76%, Enterobacter cloacea and Staphylococcus haemolyticus – 3.59%, Streptococcus dysgalactiae – 3.51%, Pasteurella multocida – 3.27%, Acinetobacter lwoffii – 2.78%, Staphylococcus cohnii – 2.61%, Bibersteinia trehalosi – 2.29%, Pseudomonas aeruginosa – 2.12%, Bacillus cereus and Micrococcus luteus – 1.96%, Corynebacterium pseudotuberculosis and Staphylococcus equorum – 1.88%, Aerococcus viridans – 1.80%, Corynebacterium xerosis – 1.72%, Clostridium perfringens, Streptococcus mitis and Streptococcus pyogenes – 1.39%, Staphylococcus chromogenes and Streptococcus entericus – 1.14%, respectively. The incidence of isolation of other types of microorganisms was below 1%. The data obtained indicate the circulation of a wide range of bacteria in goat and sheep breeding enterprises of the Russian Federation, some of which should be positioned as pathogenic flora (for example, Pasteurella multocida, Listeria monocytogenes, Salmonella enteritidis, Salmonella typhimurium, Clostridium perfringens, etc.), some as conditionally pathogenic (Trueperella pyogenes, Staphylococcus aureus, Bibersteinia trehalosi, Mannheimia haemolytica, Pseudomonas aeruginosa, Moraxella bovis, Moraxella bovoculi, etc.), as well as the normal flora of the animal body. It is worth focusing on these data when conducting a survey of livestock enterprises in order to establish an objective epizootic situation, including taking into account the possibility of circulating pathogens of factor diseases.

scholarly journals Organoselenium Coating on Cellulose Inhibits the Formation of Biofilms by Pseudomonas aeruginosa and Staphylococcus aureus

2009 ◽  
Vol 75 (11) ◽  
pp. 3586-3592 ◽  
Author(s):  
Phat L. Tran ◽  
Adrienne A. Hammond ◽  
Thomas Mosley ◽  
Janette Cortez ◽  
Tracy Gray ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Biofilm Formation ◽  
Bacterial Infections ◽  
Antimicrobial Agents ◽  
Bacterial Attachment ◽  
Wound Dressings ◽  
Aqueous Environment ◽  
Burn Victims ◽  
Surgical Wounds

ABSTRACT Among the most difficult bacterial infections encountered in treating patients are wound infections, which may occur in burn victims, patients with traumatic wounds, necrotic lesions in people with diabetes, and patients with surgical wounds. Within a wound, infecting bacteria frequently develop biofilms. Many current wound dressings are impregnated with antimicrobial agents, such as silver or antibiotics. Diffusion of the agent(s) from the dressing may damage or destroy nearby healthy tissue as well as compromise the effectiveness of the dressing. In contrast, the antimicrobial agent selenium can be covalently attached to the surfaces of a dressing, prolonging its effectiveness. We examined the effectiveness of an organoselenium coating on cellulose discs in inhibiting Pseudomonas aeruginosa and Staphylococcus aureus biofilm formation. Colony biofilm assays revealed that cellulose discs coated with organoselenium completely inhibited P. aeruginosa and S. aureus biofilm formation. Scanning electron microscopy of the cellulose discs confirmed these results. Additionally, the coating on the cellulose discs was stable and effective after a week of incubation in phosphate-buffered saline. These results demonstrate that 0.2% selenium in a coating on cellulose discs effectively inhibits bacterial attachment and biofilm formation and that, unlike other antimicrobial agents, longer periods of exposure to an aqueous environment do not compromise the effectiveness of the coating.

scholarly journals SENSIBILIDADE DA COLORAÇÃO DE GRAM NO DIAGNÓSTICO PRÉVIO DAS INFECÇÕES EM SÍTIOS CORPORAIS ESTÉREIS

2004 ◽  
Vol 5 (2) ◽  
Author(s):  
A. V. Daur ◽  
G. D. Botao ◽  
L. M. Dalla Costa ◽  
F. Klimak Jr. ◽  
C. L. B. Monteiro
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Staining Method ◽  
Biological Fluids ◽  
Etiologic Agent ◽  
Isolation And Identification ◽  
E Coli ◽  
Gram Staining ◽  
Early Diagnostic

O isolamento e identificação do agente etiológico causador de infecção a partir de líquidos biológicos podem ser um fator crítico para a recuperação da saúde do paciente, pois estas infecções geralmente são graves e deixam seqüelas. Neste trabalho foram analisadas 82 amostras de diferentes líquidos biológicos, comparando os resultados da coloração de Gram com os das culturas, além de verificar incidência dos microrganismos isolados. O Gram apresentou sensibilidade e especificidade de 62,5% e 93,9% respectivamente. Pseudomonas aeruginosa, Escherichia coli e Staphylococcus aureus foram as bactérias mais isoladas. Concluiu-se que a Coloração de Gram pode ser uma ferramenta útil na análise dos líquidos biológicos, contribuindo no diagnóstico preliminar destas infecções. SENSITIVITY OF GRAM STAINING FOR EARLY DIAGNOSTIC OF INFECTIONS IN STERILE BODY SITES Abstract Isolation and identification of an etiologic agent from biological fluids can be a critic factor for the clinical outcome of the patient, because this infection can generally be severe and cause sequels. Eighty-two different samples of biological fluids were analyzed through the Gram staining method. The results of Gram were compared with those obtained with culture, and the incidence of isolated microorganisms was also analyzed. The Gram staining presented 62.5% and 93.9% of sensitivity and specificity, respectively. Among all types of isolated and identified bacteria, P. aeruginosa, E. coli and S. aureus were the commonest ones. It was concluded that the Gram staining could be a useful tool on analysis of biological fluids, contributing for the previous diagnostic of these infections.

scholarly journals Methionine Limitation Impairs Pathogen Expansion and Biofilm Formation Capacity

2019 ◽  
Vol 85 (9) ◽  
Author(s):  
A. Jochim ◽  
T. Shi ◽  
D. Belikova ◽  
S. Schwarz ◽  
A. Peschel ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Human Serum ◽  
Bacterial Infections ◽  
Bacterial Species ◽  
Biosynthesis Pathway ◽  
Methionine Biosynthesis ◽  
Content Type

ABSTRACTMultidrug-resistant bacterial pathogens are becoming increasingly prevalent, and novel strategies to treat bacterial infections caused by these organisms are desperately needed. Bacterial central metabolism is crucial for catabolic processes and provides precursors for anabolic pathways, such as the biosynthesis of essential biomolecules like amino acids or vitamins. However, most essential pathways are not regarded as good targets for antibiotic therapy since their products might be acquired from the environment. This issue raises doubts about the essentiality of such targets during infection. A putative target in bacterial anabolism is the methionine biosynthesis pathway. In contrast to humans, almost all bacteria carry methionine biosynthesis pathways which have often been suggested as putative targets for novel anti-infectives. While the growth of methionine auxotrophic strains can be stimulated by exogenous methionine, the extracellular concentrations required by most bacterial species are unknown. Furthermore, several phenotypic characteristics of methionine auxotrophs are only partly reversed by exogenous methionine. We investigated methionine auxotrophic mutants ofStaphylococcus aureus,Pseudomonas aeruginosa, andEscherichia coli(all differing in methionine biosynthesis enzymes) and found that each needed concentrations of exogenous methionine far exceeding that reported for human serum (∼30 µM). Accordingly, these methionine auxotrophs showed a reduced ability to proliferate in human serum. Additionally,S. aureusandP. aeruginosamethionine auxotrophs were significantly impaired in their ability to form and maintain biofilms. Altogether, our data show intrinsic defects of methionine auxotrophs. This result suggests that the pathway should be considered for further studies validating the therapeutic potential of inhibitors.IMPORTANCENew antibiotics that attack novel targets are needed to circumvent widespread resistance to conventional drugs. Bacterial anabolic pathways, such as the enzymes for biosynthesis of the essential amino acid methionine, have been proposed as potential targets. However, the eligibility of enzymes in these pathways as drug targets is unclear because metabolites might be acquired from the environment to overcome inhibition. We investigated the nutritional needs of methionine auxotrophs of the pathogensStaphylococcus aureus,Pseudomonas aeruginosa, andEscherichia coli. We found that each auxotrophic strain retained a growth disadvantage at methionine concentrations mimicking those availablein vivoand showed that biofilm biomass was strongly influenced by endogenous methionine biosynthesis. Our experiments suggest that inhibition of the methionine biosynthesis pathway has deleterious effects even in the presence of external methionine. Therefore, additional efforts to validate the effects of methionine biosynthesis inhibitorsin vivoare warranted.

scholarly journals High-Sensitivity Monoclonal Antibodies Specific for Homoserine Lactones Protect Mice from Lethal Pseudomonas aeruginosa Infections

2013 ◽  
Vol 80 (2) ◽  
pp. 462-469 ◽  
Author(s):  
Soumya Palliyil ◽  
Christina Downham ◽  
Ian Broadbent ◽  
Keith Charlton ◽  
Andrew J. Porter
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Monoclonal Antibodies ◽  
Bacterial Infections ◽  
Recombinant Antibody ◽  
Cell Communication ◽  
High Sensitivity ◽  
Therapeutic Benefit ◽  
Cross Reactivity ◽  
Content Type ◽  
Homoserine Lactones

ABSTRACTA number of bacteria, including pathogens likePseudomonas aeruginosa, utilize homoserine lactones (HSLs) as quorum sensing (QS) signaling compounds and engage in cell-to-cell communication to coordinate their behavior. Blocking this bacterial communication may be an attractive strategy for infection control as QS takes a central role inP. aeruginosabiology. In this study, immunomodulation of HSL molecules by monoclonal antibodies (MAbs) was used as a novel approach to preventP. aeruginosainfections and as tools to detect HSLs in bodily fluids as a possible first clue to an undiagnosed Gram-negative infection. Using sheep immunization and recombinant antibody technology, a panel of sheep-mouse chimeric MAbs were generated which recognized HSL compounds with high sensitivity (nanomolar range) and cross-reactivity. These MAbs retained their nanomolar sensitivity in complex matrices and were able to recognize HSLs inP. aeruginosacultures grown in the presence of urine. In a nematode slow-killing assay, HSL MAbs significantly increased the survival of worms fed on the antibiotic-resistant strain PA058. The therapeutic benefit of these MAbs was further studied using a mouse model ofPseudomonasinfection in which groups of mice treated with HSL-2 and HSL-4 MAbs survived, 7 days after pathogen challenge, in significantly greater numbers (83 and 67%, respectively) compared with the control groups. This body of work has provided early proof-of-concept data to demonstrate the potential of HSL-specific, monoclonal antibodies as theranostic clinical leads suitable for the diagnosis, prevention, and treatment of life-threatening bacterial infections.

Ceramide in bacterial infections and cystic fibrosis

2008 ◽  
Vol 389 (11) ◽  
Author(s):  
Heike Grassmé ◽  
Katrin Anne Becker ◽  
Yang Zhang ◽  
Erich Gulbins
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacterial Infections ◽  
De Novo ◽  
Acid Sphingomyelinase ◽  
Temporal Organization ◽  
Host Cells ◽  
Host Responses ◽  
Mammalian Host ◽  
Membrane Domains

AbstractCeramide is formed by the activity of sphingomyelinases, by degradation of complex sphingolipids, reverse ceramidase activity orde novosynthesized. The formation of ceramide within biological membranes results in the formation of large ceramide-enriched membrane domains. These domains serve the spatial and temporal organization of receptors and signaling molecules. The acid sphingomyelinase-ceramide system plays an important role in the infection of mammalian host cells with bacterial pathogens such asNeisseria gonorrhoeae,Escherichia coli,Staphylococcus aureus,Listeria monocytogenes,Salmonella typhimuriumandPseudomonas aeruginosa. Ceramide and ceramide-enriched membrane platforms are also involved in the induction of apoptosis in infected cells, such as in epithelial and endothelial cells after infection withPseudomonas aeruginosaandStaphylococcus aureus, respectively. Finally, ceramide-enriched membrane platforms are critical regulators of the release of pro-inflammatory cytokines upon infection. The diverse functions of ceramide in bacterial infections suggest that ceramide and ceramide-enriched membrane domains are key players in host responses to many pathogens and thus are potential novel targets to treat infections.

scholarly journals Isolation and Identification of Microorganisms Growing on the Root of Leguminous Plants (Groundnut, Soya bean and Pea)

2019 ◽  
pp. 1-7
Author(s):  
Bukola Catherine Akin-Osanaiye ◽  
Oluwatobi Olaife Arowolo ◽  
Ifeyomi Wilfred Olobayotan
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Bacillus Cereus ◽  
Soya Bean ◽  
Leguminous Plant ◽  
Isolation And Identification ◽  
Leguminous Plants ◽  
And Staphylococcus Aureus

Study on the isolation and identifications of bacteria associated with the root of legumes were conducted using Spread Plate Technique. The frequencies of occurrences of the bacteria isolate showed that a total of sixteen (16) bacteria belonging to three genera and four species were isolated from the leguminous plants. Maximum number recovered from sample collected from the root of groundnut was seven (7) followed by Soya bean with five (5) while Pea recorded the least number of four (4). Role of Bacillus subtilis in the soil around the leguminous plant was the highest, which covered about 37.50% of the total isolates. Other bacteria that were also isolated from the soil around the legumes root include Bacillus cereus and Staphylococcus aureus which covered about four (4) each representing 25.0% of the total isolates while Pseudomonas aeruginosa recorded the least value of 12.50%. The bacteria isolated from the root of the legumes were not significantly different (P < 0.05). The bacteria have Nitrogen-fixing potential, having isolated from three leguminous plants which include Soya bean, Groundnut and Pea.

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