scholarly journals Investigating lead removal at trace concentrations from water by inactive yeast cells

2021 ◽  
Author(s):  
Patritsia M. Stathatou ◽  
Christos E. Athanasiou ◽  
Marios Tsezos ◽  
John W Goss ◽  
Camron Blackburn ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Cell Wall ◽  
Emerging Contaminants ◽  
Mass Transfer Process ◽  
Yeast Cells ◽  
Lead Removal ◽  
Toxic Heavy Metals ◽  
Yeast Saccharomyces Cerevisiae ◽  
Efficient Manner ◽  
Wall Stiffness

Traces of heavy metals found in water resources, due to mining activities and e-waste discharge, pose a global threat. Conventional treatment processes fail to remove toxic heavy metals, such as lead, from drinking water in a resource-efficient manner when their initial concentrations are low. Here, we show that by using the yeast Saccharomyces cerevisiae we can effectively remove trace lead from water via a rapid mass transfer process, achieving an uptake of up to 12 mg lead per gram of biomass in solutions with initial lead concentrations below 1 part per million. We found that the yeast cell wall plays a crucial role in this process, with its mannoproteins and β-glucans being the key potential lead adsorbents. Furthermore, we discovered that biosorption is linked to a significant increase in cell wall stiffness. These findings open new opportunities for using environmentally friendly and abundant biomaterials for advanced water treatment targeting emerging contaminants.

scholarly journals Purification of profilin from Saccharomyces cerevisiae and analysis of profilin-deficient cells.

1990 ◽  
Vol 110 (1) ◽  
pp. 105-114 ◽  
Author(s):  
B K Haarer ◽  
S H Lillie ◽  
A E Adams ◽  
V Magdolen ◽  
W Bandlow ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Wall ◽  
Actin Polymerization ◽  
Yeast Cells ◽  
Predicted Amino Acid Sequence ◽  
Temperature Sensitive ◽  
Wild Type ◽  
Yeast Saccharomyces Cerevisiae ◽  
Ellipsoidal Shape ◽  
Hydrolysis Of

We have isolated profilin from yeast (Saccharomyces cerevisiae) and have microsequenced a portion of the protein to confirm its identity; the region microsequenced agrees with the predicted amino acid sequence from a profilin gene recently isolated from S. cerevisiae (Magdolen, V., U. Oechsner, G. Müller, and W. Bandlow. 1988. Mol. Cell. Biol. 8:5108-5115). Yeast profilin resembles profilins from other organisms in molecular mass and in the ability to bind to polyproline, retard the rate of actin polymerization, and inhibit hydrolysis of ATP by monomeric actin. Using strains that carry disruptions or deletions of the profilin gene, we have found that, under appropriate conditions, cells can survive without detectable profilin. Such cells grow slowly, are temperature sensitive, lose the normal ellipsoidal shape of yeast cells, often become multinucleate, and generally grow much larger than wild-type cells. In addition, these cells exhibit delocalized deposition of cell wall chitin and have dramatically altered actin distributions.

scholarly journals The Activity of Cell-Wall Modifying β-1,3-Glucanases in Soybean Grown in Presence of Heavy Metals

2016 ◽  
Vol 15 (2) ◽  
pp. 114-121
Author(s):  
Monika Bardáčová ◽  
Marína Maglovski ◽  
Zuzana Gregorová ◽  
Yevheniia Konotop ◽  
Miroslav Horník ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Cell Wall ◽  
Cell Walls ◽  
Regulation Mechanism ◽  
Soybean Variety ◽  
Toxic Heavy Metals ◽  
Metal Sequestration ◽  
Enzyme Isoforms ◽  
Wall Permeability ◽  
Cell Wall Permeability

AbstractCell walls represent the first barrier that can prevent the entrance of toxic heavy metals into plants. The composition and the flexibility of the cell wall are regulated by different enzymes. The ß-1,3-glucanases control the degradation of the polysaccharide callose as a flexible regulation mechanism of cell wall permeability and/or its ability to bind metals under stress conditions. The profile and activity of ß-1,3-glucanases in the presence of heavy metals, however, has rarely been studied. Here we studied these enzymes in four soybean varieties (Glycine max) grown in the presence of cadmium ions. These analyses revealed three acidic and one basic enzyme isoforms in each soybean variety, but only two of the acidic isoforms in the variety Moravians were substantially responsive to the presence of Cd2+. Since the responses of certain glucanases were detected mainly in the varieties sensitive to metal and accumulating high amounts of metals, we assume their role in the defense rather than strategic metal sequestration.

Combining mussel and seaweed hydrogel-inspired strategies to design novel ion-imprinted sorbents for ultra-efficient lead removal from water

2019 ◽  
Vol 43 (14) ◽  
pp. 5495-5502 ◽  
Author(s):  
Wei Shen ◽  
Xiao Jiang ◽  
Qing-Da An ◽  
Zuo-Yi Xiao ◽  
Shang-Ru Zhai ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Human Health ◽  
Lead Removal ◽  
Toxic Heavy Metals ◽  
Ion Imprinted

Lead(ii) is one of the most toxic heavy metals and is a serious threat to the environment and human health.

The influence of heavy metals on the production of extracellular polymer substances in the processes of heavy metal ions elimination

2005 ◽  
Vol 52 (10-11) ◽  
pp. 151-156 ◽  
Author(s):  
J. Mikes ◽  
M. Siglova ◽  
A. Cejkova ◽  
J. Masak ◽  
V. Jirku
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Cell Wall ◽  
Metal Ions ◽  
Cell Walls ◽  
Heavy Metal Ions ◽  
Extracellular Polymeric Substances ◽  
Low Cost ◽  
Toxic Heavy Metals ◽  
Metal Interactions

Wastewaters from a chemical industry polluted by heavy metal ions represent a hazard for all living organisms. It can mean danger for ecosystems and human health. New methods are sought alternative to traditional chemical and physical processes. Active elimination process of heavy metals ions provided by living cells, their components and extracellular products represents a potential way of separating toxic heavy metals from industrial wastewaters. While the abilities of bacteria to remove metal ions in solution are extensively used, fungi have been recognized as a promising kind of low-cost adsorbents for removal of heavy-metal ions from aqueous waste sources. Yeasts and fungi differ from each other in their constitution and in their abilities to produce variety of extracellular polymeric substances (EPS) with different mechanisms of metal interactions. The accumulation of Cd(2+), Cr(6+), Pb(2+), Ni(2+) and Zn(2+) by yeasts and their EPS was screened at twelve different yeast species in microcultivation system Bioscreen C and in the shaking Erlenmayer's flasks. This results were compared with the production of yeast EPS and the composition of yeast cell walls. The EPS production was measured during the yeast growth and cell wall composition was studied during the cultivations in the shaking flasks. At the end of the process extracellular polymers and their chemical composition were isolated and amount of bound heavy metals was characterized. The variable composition and the amount of the EPS were found at various yeast strains. It was influenced by various compositions of growth medium and also by various concentrations of heavy metals. It is evident, that the amount of bound heavy metals was different. The work reviews the possibilities of usage of various yeast EPS and components of cell walls in the elimination processes of heavy metal ions. Further the structure and properties of yeasts cell wall and EPS were discussed. The finding of mechanisms mentioned above is necessary to identify the functional groups entered in the metals elimination processes.

scholarly journals Significant quantities of the glycolytic enzyme phosphoglycerate mutase are present in the cell wall of yeast Saccharomyces cerevisiae

2003 ◽  
Vol 369 (2) ◽  
pp. 357-362 ◽  
Author(s):  
Precious MOTSHWENE ◽  
Wolf BRANDT ◽  
George LINDSEY
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Wall ◽  
Cell Walls ◽  
Glycolytic Enzyme ◽  
Yeast Cells ◽  
Phosphoglycerate Mutase ◽  
Elevated Concentration ◽  
Yeast Saccharomyces Cerevisiae ◽  
Normal Morphology ◽  
Immunocytochemical Analysis

NaOH was used to extract proteins from the cell walls of the yeast Saccharomyces cerevisiae. This treatment was shown not to disrupt yeast cells, as NaOH-extracted cells displayed a normal morphology upon electron microscopy. Moreover, extracted and untreated cells had qualitatively similar protein contents upon disruption. When yeast was grown in the presence of 1M mannitol, two proteins were found to be present at an elevated concentration in the cell wall. These were found to be the late-embryogenic-abundant-like protein heat-shock protein 12 and the glycolytic enzyme phosphoglycerate mutase. The presence of phosphoglycerate mutase in the cell wall was confirmed by immunocytochemical analysis. Not only was the phosphoglycerate mutase in the yeast cell wall found to be active, but whole yeast cells were also able to convert 3-phosphoglycerate in the medium into ethanol, provided that the necessary cofactors were present.

Li+ effect on the cell wall of the yeast Saccharomyces cerevisiae as probed by FT-IR spectroscopy

2013 ◽  
Vol 8 (8) ◽  
pp. 724-729 ◽  
Author(s):  
Aurelijus Zimkus ◽  
Audrius Misiūnas ◽  
Larisa Chaustova
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Wall ◽  
Ir Spectroscopy ◽  
Absorption Band ◽  
Structural Changes ◽  
Yeast Cells ◽  
Wall Structure ◽  
Yeast Saccharomyces Cerevisiae ◽  
Ft Ir ◽  
Ft Ir Spectroscopy

AbstractThe effect of Li+ ions as a transformation inducing agent on the yeast cell wall has been studied. Two Saccharomyces cerevisiae strains, p63-DC5 with a native cell wall, and strain XCY42-30D(mnn1) which contains structural changes in the mannan-protein complex, were used. Fourier transform infrared (FT-IR) spectroscopy has been used for the characterization of the yeast strains and for determination of the effect of lithium cations on the cell wall. A comparison of the carbohydrate absorption band positions in the 970–1185 cm−1 range, of Na+ and Li+ treated yeast cells has been estimated. Absorption band positions of the cell wall carbohydrates of p63-DC5 were not influenced by the studied ions. On the contrary, the treatment of XCY42-30D(mnn1) cells with Li+ ions shifted glucan band positions, implying that the cell wall structure of strain XCY42-30D(mnn1) is more sensitive to Li+ ion treatment.

scholarly journals Glucose-induced sequential processing of a glycosyl-phosphatidylinositol-anchored ectoprotein in Saccharomyces cerevisiae.

1996 ◽  
Vol 16 (1) ◽  
pp. 442-456 ◽  
Author(s):  
G Müller ◽  
E Gross ◽  
S Wied ◽  
W Bandlow
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Wall ◽  
Plasma Membrane ◽  
Yeast Cells ◽  
Yeast Saccharomyces Cerevisiae ◽  
Glycosyl Phosphatidylinositol ◽  
Sequential Processing ◽  
Protein Label ◽  
Covalently Linked

Transfer of spheroplasts from the yeast Saccharomyces cerevisiae to glucose leads to the activation of an endogenous (glycosyl)-phosphatidylinositol-specific phospholipase C ([G]PI-PLC), which cleaves the anchor of at least one glycosyl-phosphatidylinositol (GPI)-anchored protein, the cyclic AMP (cAMP)-binding ectoprotein Gce1p (G. Müller and W. Bandlow, J. Cell Biol. 122:325-336, 1993). Analyses of the turnover of two constituents of the anchor, myo-inositol and ethanolamine, relative to the protein label as well as separation of the two differently processed versions of Gce1p by isoelectric focusing in spheroplasts demonstrate the glucose-induced conversion of amphiphilic Gce1p first into a lipolytically cleaved hydrophilic intermediate, which is then processed into another hydrophilic version lacking both myo-inositol and ethanolamine. When incubated with unlabeled spheroplasts, the lipolytically cleaved intermediate prepared in vitro is converted into the version lacking all anchor constituents, whereby the anchor glycan is apparently removed as a whole. The secondary cleavage ensues independently of the carbon source, attributing the key role in glucose-induced anchor processing to the endogenous (G)PI-PLC. The secondary processing of the lipolytically cleaved intermediate of Gce1p at the plasma membrane is correlated with the emergence of a covalently linked high-molecular-weight form of a cAMP-binding protein at the cell wall. This protein lacks anchor components, and its protein moiety appears to be identical with double-processed Gce1p detectable at the plasma membrane in spheroplasts. The data suggest that glucose-induced double processing of GPI anchors represents part of a mechanism of regulated cell wall expression of proteins in yeast cells.

scholarly journals LEA (late embryonic abundant)-like protein Hsp 12 (heat-shock protein 12) is present in the cell wall and enhances the barotolerance of the yeast Saccharomyces cerevisiae

2004 ◽  
Vol 377 (3) ◽  
pp. 769-774 ◽  
Author(s):  
Precious MOTSHWENE ◽  
Robert KARREMAN ◽  
Gail KGARI ◽  
Wolf BRANDT ◽  
George LINDSEY
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Wall ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Barometric Pressure ◽  
Yeast Cells ◽  
Wild Type ◽  
Yeast Saccharomyces Cerevisiae ◽  
Rapid Changes ◽  
Wild Type Yeast

Yeast cells Saccharomyces cerevisiae, late embryogenic abundant-like stress response protein Hsp 12 (heat-shock protein 12) were found by immunocytochemistry to be located both in the cytoplasm and in the cell wall, from where they could be extracted with dilute NaOH solutions. Yeast cells with the Hsp 12 gene disrupted were unable to grow in the presence of either 12 mM caffeine or 0.43 mM Congo Red, molecules known to affect cell-wall integrity. The volume of yeast cells were less affected by rapid changes in the osmolality of the growth medium when compared with the wild-type yeast cells, suggesting a role for Hsp 12 in the flexibility of the cell wall. This was also suggested by subjecting the yeast cells to rapid changes in barometric pressure where it was found that wild-type yeast cells were more resistant to cellular breakage.

ASSESSMENT OF HEAVY METAL CONCENTRATION IN COCONUT WATER

1970 ◽  
pp. 07-10
Author(s):  
MdDidarul Islam, Ashiqur Rahaman, Aboni Afrose
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Heavy Metal Concentration ◽  
Coconut Water ◽  
Toxic Heavy Metals ◽  
High Concentration ◽  
Toxic Heavy Metal ◽  
Low Concentration ◽  
Wet Ashing ◽  
Aas Method

This study was based on determining concentration of essential and toxic heavy metal in coconut water available at a local Hazaribagh area in Dhaka, Bangladesh. All essential minerals, if present in the drinking water at high concentration or very low concentration, it has negative actions. In this study, fifteen samples and eight heavy metals were analyzed by Atomic Absorption Spectroscopy (AAS) method which was followed by wet ashing digestion method. The concentration obtained in mg/l were in the range of 0.3 to 1.5, 7.77 to 21.2, 0 to 0.71, 0 to 0.9, 0 to 0.2, 0.9 to 17.3, 0.1 to 0.9, 0 to 0.9 and 0 to 0.7 for Fe, Ni, Cu, Cd, Cr, Zn, Pb and Se respectively. From this data it was concluded that any toxic heavy metals like Cd, Cr, Pb and Ni exceed their toxicity level and some essential nutrients were in low concentration in those samples. 

Assessment of micro and macro nutrients in poultry feeds available in Dhaka city, Bangladesh.

2017 ◽  
Vol 1 ◽  
pp. 264
Author(s):  
Md Didarul Islam ◽  
Ashiqur Rahaman ◽  
Fahmida Jannat
Keyword(s):  
Heavy Metals ◽  
Adverse Effect ◽  
The Other ◽  
Nutrient Level ◽  
Dhaka City ◽  
Toxic Heavy Metals ◽  
High Concentration ◽  
Low Concentration ◽  
Feed Formulation ◽  
Macro Nutrients

This study was based on to determine the concentration of macro and micro nutrients as well as toxic and nontoxic heavy metals present in the chicken feed available in Dhaka city of Bangladesh. All macro nutrients, if present in the feed at high concentration have some adverse effect, at the same time if this nutrient present in the feed at low concentration this have some adverse effect too. So that this nutrient level should be maintained at a marginal level. On the other side toxic heavy metals if present in the feed at very low concentration those can contaminate the total environment of the ecosystem. In this study six brand samples (starter, grower, finisher and layer) which was collected from different renowned chicken feed formulation industry in Bangladesh. Those samples were prepared for analysis by wet ashing and then metals were determined by Atomic Absorption Spectroscopy. It was found that 27.7 to 68.4, 57.3 to 121.9, 0.21 to 4.1, 0.32 to 2.1, 0.11 to 1.58, 0.28 to 2.11 and 0.28 to 1.78 for zinc, iron, copper, mercury, cadmium, nickel and cobalt respectively. It was found that essential macro and micro nutrients were present in the feed in low concentration on the other side mercury was present in high concentration in the feed samples.

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