Word- and Text-Level Processes Contributing to Fluent Reading of Word Lists and Sentences

In this study, we investigated how word- and text-level processes contribute to different types of reading fluency measures. We aimed to increase our understanding of the underlying processes necessary for fluent reading. The sample included 73 Dutch Grade 3 children, who were assessed on serial word reading rate (familiar words), word-list reading fluency (increasingly difficult words), and sentence reading fluency. Word-level processes were individual word recognition speed (discrete word reading) and sequential processing efficiency (serial digit naming). Text-level processes were receptive vocabulary and syntactic skills. The results showed that word- and text-level processes combined accounted for a comparable amount of variance in all fluency outcomes. Both word-level processes were moderate predictors of all fluency outcomes. However, vocabulary only moderately predicted sentence reading fluency, and syntactic skills merely contributed to sentence reading fluency indirectly through vocabulary. The findings indicate that sequential processing efficiency has a crucial role in reading fluency across various measures besides individual word recognition speed. Additionally, text-level processes come into play when complexity and context availability of fluency measures increases, but the exact timing requires further study. Findings are discussed in terms of future directions and their possible value for diagnostic assessment and intervention of reading difficulties.

A Method and Device for Automated Grinding of Small Ceramic Elements

The paper describes an automated method for grinding small ceramic elements using a hyperboloid wheel. The problem of automating the process of machining elements made of nonmagnetic materials with a small area and low height has been solved. Automation of the grinding process was possible thanks to automatic clamping of workpieces in the machining zone and sequential processing by a specified number of grinding wheels. The workpieces were passed through successive machining zones. The division of the allowance of individual grinding wheels was made taking into account the characteristics of the workpieces and the requirements for the results of the machining. Obtaining a long grinding zone and the effect of automatic clamping of the workpieces was possible due to the inclination of the grinding wheel axis in relation to the plane of movement of the workpieces. Innovative aggregate grinding wheels were used for grinding. The aggregates containing diamond abrasive grains, connected with a metal bond, were embedded in the porous structure of the resin bond. The aggregates ensured high efficiency of grinding, and their developed surface contributed to good holding in the resin binder. The durability of grinding wheels was 64 h, which enables the machining of 76,000 ceramic elements.

Ultrasound and Ozone Processing of Cashew Apple Juice: Effects of Single and Combined Processing on the Juice Quality and Microbial Stability

Standalone and sequential ultrasound (US) and ozone (OZ) processes were applied to cashew apple juice. An unsupervised method, by principal component analysis (PCA), was used to understand the effect of the non-thermal treatments on the cashew apple composition. The US processing (373 W/cm2; 10 min; 40 °C) promoted the highest peroxidase inactivation and increased the flavonoid content and antioxidant activity (DPPH ABTS and FRAP methods). The ozone processing (0.24 mg O3/mL) increased total phenolic compounds (TPC). Sequential processing was carried out by applying both of the processes, using the best processing conditions for US and OZ. Sequential processing resulted in the higher retention of yellow flavonoids than in the control and single processing. However, the effect of sequential US and OZ processing can be deleterious to vitamin C and TPC after 30 days of cold storage while maintaining the flavonoids of the cashew apple juice. Furthermore, the synergy between US and OZ reduced native microbiota counts, suggesting a microbiologically safe juice. Further studies on the mechanism of bioactive compound degradation might be helpful for product quality modulation.

Spatial attention in encoding letter combinations

Reading requires the correct identification of letters and letter positions within words. Selective attention is, therefore, required to select chunks of the text for sequential processing. Despite the extensive literature on visual attention, the well-known effects of spatial cues in simple perceptual tasks cannot inform us about the role of attention in a task as complex as reading. Here, we systematically manipulate spatial attention in a multi-letter processing task to understand the effects of spatial cues on letter encoding in typical adults. Overall, endogenous (voluntary) cue benefits were larger than exogenous (reflexive). We show that cue benefits are greater in the left than in the right visual field and larger for the most crowded letter positions. Endogenous valid cues reduced errors due to confusing letter positions more than misidentifications, specifically for the most crowded letter positions. Therefore, shifting endogenous attention along a line of text is likely an important mechanism to alleviate the effects of crowding on encoding letters within words. Our results help set the premise for constructing theories about how specific mechanisms of attention support reading development in children. Understanding the link between reading development and attention mechanisms has far-reaching implications for effectively addressing the needs of children with reading disabilities.

The HIV-1 Nucleocapsid Regulates Its Own Condensation by Phase-Separated Activity-Enhancing Sequestration of the Viral Protease during Maturation

A growing number of studies indicate that mRNAs and long ncRNAs can affect protein populations by assembling dynamic ribonucleoprotein (RNP) granules. These phase-separated molecular ‘sponges’, stabilized by quinary (transient and weak) interactions, control proteins involved in numerous biological functions. Retroviruses such as HIV-1 form by self-assembly when their genomic RNA (gRNA) traps Gag and GagPol polyprotein precursors. Infectivity requires extracellular budding of the particle followed by maturation, an ordered processing of ∼2400 Gag and ∼120 GagPol by the viral protease (PR). This leads to a condensed gRNA-NCp7 nucleocapsid and a CAp24-self-assembled capsid surrounding the RNP. The choreography by which all of these components dynamically interact during virus maturation is one of the missing milestones to fully depict the HIV life cycle. Here, we describe how HIV-1 has evolved a dynamic RNP granule with successive weak–strong–moderate quinary NC-gRNA networks during the sequential processing of the GagNC domain. We also reveal two palindromic RNA-binding triads on NC, KxxFxxQ and QxxFxxK, that provide quinary NC-gRNA interactions. Consequently, the nucleocapsid complex appears properly aggregated for capsid reassembly and reverse transcription, mandatory processes for viral infectivity. We show that PR is sequestered within this RNP and drives its maturation/condensation within minutes, this process being most effective at the end of budding. We anticipate such findings will stimulate further investigations of quinary interactions and emergent mechanisms in crowded environments throughout the wide and growing array of RNP granules.

Modeling and performance analysis of shuttle-based compact storage systems under parallel processing policy

Short response time for order processing is important for modern warehouses, which can be potentially achieved by adopting appropriate processing policy. The parallel processing policy have advantages in improving performance of many autonomous storage and retrieval systems. However, researchers tend to assume a sequential processing policy managing the movement of independent resources in shuttle-based compact storage systems. This paper models and analyses a single-tier of specialized shuttle-based compact storage systems under parallel processing policy. The system is modeled as a semi-open queueing network with class switching and the parallel movement of shuttles and the transfer car is modeled using a fork-join queueing network. The analytical model is validated against simulations and the results show our model can accurately estimate the system performance. Numerical experiments and a real case are carried out to compare the performance of parallel and sequential processing policies. The results suggest a critical transaction arrival rate and depth/width ratio, below which the sequential processing policy outperforms the parallel processing policy. However, the advantage of sequential processing policy is decreasing with the increasing of shuttle number, transaction arrival rate and depth/width ratio. The results also suggest an optimal depth/width ratio with a value of 1.75 for minimizing the expected throughput time in the real system. Given the current system configurations, the parallel processing policy should be considered when the number of shuttles is larger than 2 or the transaction arrival rate is larger than 24 per hour.

A Service for Parallel Processing of Protected Information Queries in a Multi-Server Environment

The principles of organizing an Internet service designed to implement parallel processing of protected queries in distributed information systems that are oriented to work in complex network environments with many processing servers are considered. Distinctive feature of the service consists in a possibility to combine sequential ("pipelined") and parallel query processing in a multi-server environment. The service architecture is based on the concept of a "Protected message" corresponding to a container for electronic documents (information query or response) that can be provided with one or more electronic digital signatures. In contrast, for example, to the Web services technology, the described service is based not on the model of calling methods of remote objects, but on the message exchange model. In this case, this means that all service processing functions (methods) have the same strict specification: they receive an array of objects of the "Protected message" program class as a parameter and return an array of objects of the same class. In sequential processing, both arrays usually contain one "Protected message" object each. However, when using parallel processing, arrays can contain multiple elements that correspond to the results of processing by multiple software threads. These processing functions are grouped into one or more dynamic libraries, which are connected to the PMS server when it starts working (each library can be considered as a remote analogue of a Web service), and become available to clients.

EEG microstate analysis of emotion regulation reveals no sequential processing of valence and emotional arousal

In electroencephalography (EEG), microstates are distributions of activity across the scalp that persist for several tens of milliseconds before changing into a different pattern. Microstate analysis is a way of utilizing EEG as both temporal and spatial imaging tool, but has rarely been applied to task-based data. This study aimed to conceptually replicate microstate findings of valence and emotional arousal processing and investigate the effects of emotion regulation on microstates, using data of an EEG paradigm with 107 healthy adults who actively viewed emotional pictures, cognitively detached from them, or suppressed facial reactions. Within the first 600 ms after stimulus onset only the comparison of viewing positive and negative pictures yielded significant results, caused by different electrodes depending on the microstate. Since the microstates associated with more and less emotionally arousing pictures did not differ, sequential processing could not be replicated. When extending the analysis to 2000 ms after stimulus onset, differences were exclusive to the comparison of viewing and detaching from negative pictures. Intriguingly, we observed the novel phenomenon of a microstate difference that could not be attributed to single electrodes. This suggests that microstate analysis can detect differences beyond those detected by event-related potential analysis.

Quantifying the Generation Process of Multi-Level Tactile Sensations via ERP Component Investigation

Humans obtain characteristic information such as texture and weight of external objects, relying on the brain’s integration and classification of tactile information; however, the decoding mechanism of multi-level tactile information is relatively elusive from the temporal sequence. In this paper, nonvariant frequency, along with the variant pulse width of electrotactile stimulus, was performed to generate multi-level pressure sensation. Event-related potentials (ERPs) were measured to investigate the mechanism of whole temporal tactile processing. Five ERP components, containing P100–N140–P200–N200–P300, were observed. By establishing the relationship between stimulation parameters and ERP component amplitudes, we found the following: (1) P200 is the most significant component for distinguishing multi-level tactile sensations; (2) P300 is correlated well with the subjective judgment of tactile sensation. The temporal sequence of brain topographies was implemented to clarify the spatiotemporal characteristics of the tactile process, which conformed to the serial processing model in neurophysiology and cortical network response area described by fMRI. Our results can help further clarify the mechanism of tactile sequential processing, which can be applied to improve the tactile BCI performance, sensory enhancement, and clinical diagnosis for doctors to evaluate the tactile process disorders by examining the temporal ERP components.