scholarly journals Transcriptome Analysis of Four Arabidopsis thaliana Mediator Tail Mutants Reveals Overlapping and Unique Functions in Gene Regulation

2018 ◽  
Author(s):  
Whitney L. Dolan ◽  
Clint Chapple

ABSTRACTThe Mediator complex is a central component of transcriptional regulation in Eukaryotes. The complex is structurally divided into four modules known as the head, middle, tail and kinase modules, and in Arabidopsis thaliana, comprises 28-34 subunits. Here, we explore the functions of four Arabidopsis Mediator tail subunits, MED2, MED5a/b, MED16, and MED23, by comparing the impact of mutations in each on the Arabidopsis transcriptome. We find that these subunits affect both unique and overlapping sets of genes, providing insight into the functional and structural relationships between them. The mutants primarily exhibit changes in the expression of genes related to biotic and abiotic stress. We find evidence for a tissue specific role for MED23, as well as in the production of alternative transcripts. Together, our data help disentangle the individual contributions of these MED subunits to global gene expression and suggest new avenues for future research into their functions.

2020 ◽  
pp. 349-366
Author(s):  
Stephen A. Kowalewski

This concluding chapter seeks to tie the individual contributions to this volume into a coherent whole. Drawing on points raised in the various chapters, it summarizes critical data and provides a synthesis of the current research presented in constituent chapters that highlight spatial, temporal, and cultural diversity in ancient west Mexico. The author offers comments and observations on all chapters, highlighting common threads and lines of thought or evidence that bind the individual chapters together into a complete dialogue. It emphasizes the significance of the evidence from the west—presented in the volume—in crafting a more complex and nuanced understanding of both the west in particular and Mesoamerica as a whole. The chapter concludes by offering thoughts and suggestions for how future research may enhance archaeological understanding of this vast and understudied region.


Author(s):  
Jessica M. Brooks ◽  
Kanako Iwanaga ◽  
Fong Chan

Arthritis is ranked among the top causes of disability in the United States and worldwide. Despite recent improvements in medications and medical treatment, there is no known cure for arthritis. Providing evidence-based psychoeducation and counseling services to people with arthritis lessens the impact of pain-related symptoms and disability on the individual and society. The purpose of this chapter is to provide an overview of the most common arthritic conditions, co-occurring physical conditions, and psychosocial factors associated with arthritis. Barriers to self-management and existing self-management programs are also discussed along with the current state of scientific evidence. The chapter concludes with some questions for future research.


Author(s):  
Nicole B. Ellison

This chapter examines the state of the art in telework research. The author reviews the most central scholarly literature examining the phenomenon of telework (also called home-based work or telecommuting) and develops a framework for organizing this body of work. She organizes previous research on telework into six major thematic concerns relating to the definition, measurement, and scope of telework; management of teleworkers; travel-related impacts of telework; organizational culture and employee isolation; boundaries between “home” and “work” and the impact of telework on the individual and the family. Areas for future research are suggested.


2017 ◽  
Vol 57 (7) ◽  
pp. 856-870 ◽  
Author(s):  
Jong-Hyeong Kim

The current study develops a theoretical model of the effect of memorable tourism experiences (MTEs) on behavioral intentions by examining the structural relationships between destination image (DI), tourist satisfaction, revisit intention, and word-of-mouth (WOM) publicity. The results show that MTEs influence future behavioral intentions both directly and indirectly through DI and tourist satisfaction. Moreover, MTEs are found to be the most influential determinant of behavioral intentions. Thus, the results challenge those practitioners and researchers who perceive visitor satisfaction to be the most important indicator of destination performance. Theoretical and managerial implications are discussed based on the study results, and directions for future research are provided.


2015 ◽  
Vol 44 (2) ◽  
pp. 658-681 ◽  
Author(s):  
Gerardus J. M. Lucas ◽  
Joris Knoben ◽  
Marius T. H. Meeus

In this paper, we study to what extent inconsistent feedback signals about performance affect firm adaptive behavior in terms of changes made to research-and-development (R&D) investments. We argue that inconsistency in performance feedback—based on discrepancies between two distinct performance signals—affects the degree to which such investments will be changed. Our aim is to show that accounting for inconsistent performance feedback is necessary as predictions for the direction of change in R&D investments based on the individual performance feedback signals are contradictory. Furthermore, we contribute by proposing a holistic consideration mechanism as an alternative to the selective attention mechanism previously applied to inconsistent performance feedback. Our findings show that the impact of inconsistency depends on the exact configuration of the underlying performance feedback signal discrepancies. While consistently negative performance feedback signals would amplify their impact in stimulating increased R&D investments, inconsistent performance feedback signals created more nuanced effects. Having lower performance compared to an industry-based peer group—despite doing well compared to the previous year—made firms decrease their R&D investments. For the opposite case of inconsistent performance feedback, we did not find an effect on change in R&D investments. These findings support to a degree our contention that explaining the effects of inconsistent performance feedback requires a holistic consideration theoretical mechanism instead of one involving selective attention. In sum, these findings suggest future research should take into account the differences between distinct instances of inconsistent performance feedback.


2015 ◽  
Vol 47 (4) ◽  
pp. 113-128 ◽  
Author(s):  
Theresa Casey ◽  
Osman V. Patel ◽  
Karen Plaut

Few studies have investigated the impact of alterations in gravity on mammalian transcriptomes. Here, we describe the impact of spaceflight on mammary transcriptome of late pregnant rats and the effect of hypergravity exposure on mammary, liver, and adipose transcriptomes in late pregnancy and at the onset of lactation. RNA was isolated from mammary collected on pregnancy day 20 from rats exposed to spaceflight from days 11 to 20 of gestation. To measure the impact of hypergravity on mammary, liver, and adipose transcriptomes we isolated RNA from tissues collected on P20 and lactation day 1 from rats exposed to hypergravity beginning on pregnancy day 9. Gene expression was measured with Affymetrix GeneChips. Microarray analysis of variance revealed alterations in gravity affected the expression of genes that regulate circadian clocks and activate mechanotransduction pathways. Changes in these systems may explain global gene expression changes in immune response, metabolism, and cell proliferation. Expression of genes that modify chromatin structure and methylation was affected, suggesting adaptation to gravity alterations may proceed through epigenetic change. Altered gravity experiments offer insights into the role of forces omnipresent on Earth that shape genomes in heritable ways. Our study is the first to analyze the impact of alterations in gravity on transcriptomes of pregnant and lactating mammals. Findings provide insight into systems that sense gravity and the way in which they affect phenotype, as well as the possibility of sustaining life beyond Earth's orbit.


2021 ◽  
Vol 29 (2) ◽  
pp. 73-82
Author(s):  
Julia Ganner ◽  
Lukas Kerschbaumer ◽  
Christina Tanzer

Purpose: The insurmountable tensions and turmoil caused by the COVID-19 pandemic in welfare systems worldwide demand governmental as well as non-governmental support, especially from the volunteer sector, which can be a powerful resource for mitigating the pandemic’s impacts. To identify ways of mobilising the enormous human resources of the baby boomer generation in particular, whose members are currently on the brink of entering retirement, the factors that have enabled and restricted volunteer management during the pandemic in Tyrol, Austria are examined. Design/Method/Approach: Following a qualitative approach, the authors performed 27 problem-centred interviews with representative senior citizens, retirees and individuals about to retire and companies in Tyrol. The authors evaluated the data in qualitative content analysis. Findings: Self-determination, time flexibility, acceptance of volunteer work in one’s social network and previous personal experience with volunteering are key determinants of sustainable volunteer work amongst retirees. Companies and a well-established acquisition management strategy also play a significant role in promoting volunteer work. Practical Implications and Originality/Value: The study involves a holistic analysis of volunteer work at the individual and organisational levels. By capturing the potential of e-volunteering and how it improves the capacities of classic face-to-face volunteer work, it can support the development of more resilient infrastructures for supporting volunteer work. Research Limitations/Future Research: The interpretation of visual and non-verbal signals was difficult due to the use of phone and online interviews, and the results should not be generalised. Even so, our findings pave the way for future studies on mechanisms determining virtual volunteering and volunteer management. Paper type: Empirical


2021 ◽  
Author(s):  
◽  
Sarah Elizabeth Burke

<p>This thesis adopted a unique approach to the exploration of proactive socialisation and the processes by which a newcomer moves from organisational outsider to insider. Although socialisation involves actions by the individual, the work group, and the organisation, this study is one of the first to investigate how these actions work in tandem to support the adjustment of organisational newcomers. Research was conducted with a group of 526 participants, drawn from a pool of New Zealand Police (NZ Police) recruits and graduate employees. A quantitative method for data gathering was adopted, with questionnaires administered over a 15-month period for police recruits and 6-month period for graduate newcomers. Results indicated that prior work quality and quantity, job interest, proactive personality, team support, and leader-member exchange each had an important role to play in the prediction of newcomer role breadth self-efficacy. In turn, newcomers who felt confident in their ability to carry out a broader and more proactive role also enjoyed a higher level of task mastery and group fit. The successful achievement of these proximal outcomes led to other, more distal outcomes, namely performance and organisational commitment. Each of these outcomes was achieved, regardless of the socialising tactics employed by the hiring organisation. An important feature of this thesis was the design and delivery of a training intervention that was aimed at coaching newcomers in a range of proactive behaviours (i.e., information-seeking, feedback-seeking, positive framing, relationship building, networking, listening, and observation/modeling). Results found that the longitudinal pattern of proaction differed for newcomers in response to the socialising tactics adopted by the organisation. Results also indicated that the impact of training on future proaction was most potent for individuals who already had an elevated level of role breadth self-efficacy, thereby pointing to the importance of building an employee' perception of their own capability. Training was also most effective when key messages were repeated over multiple sessions, and integrated into the solving of realworld tasks. These results challenge previous studies that have assumed proactivity to be a stable construct over time. Beyond contributing to the literature on newcomer socialisation, this thesis goes some way to clarifying why proactive people actually succeed. It would seem that proactive people expect to be successful, thereby making a training intervention more useful. This thesis also challenges prior research that assumes certain adjustment outcomes are dependent on the socialising tactics adopted by the hiring organisation. Thus, rather than passively adapt to their environment, this research shows how a newcomer can actively shape their own socialising experience. A number of methodological weaknesses found in previous studies have been addressed in this thesis. It also presents a number of practical implications to support the pre-entry, initial entry, and long-term adjustment of seasoned newcomers, versus graduate Generation Y employees. Multiple options for future research are also considered.</p>


2020 ◽  
Author(s):  
Javier Canales ◽  
Felipe Uribe ◽  
Carlos Henríquez-Valencia ◽  
Carlos Lovazzano ◽  
Joaquín Medina ◽  
...  

Abstract Background: Sulfur is a major component of biological molecules and thus an essential element for plants. Deficiency of sulfate, the main source of sulfur in soils, negatively influences plant growth and crop yield. The effect of sulfate deficiency on plants has been well characterized at the physiological, transcriptomic and metabolomic levels in Arabidopsis thaliana and a limited number of crop plants. However, we still lack a thorough understanding of the molecular mechanisms and regulatory networks underlying sulfate deficiency in most plants. In this work we analyzed the impact of sulfate starvation on the transcriptome of tomato plants to identify regulatory networks and key transcriptional regulators at a temporal and organ scale. Results: Sulfate starvation reduces the growth of roots and leaves which is accompanied by major changes in the organ transcriptome, with the response being temporally earlier in roots than leaves. Comparative analysis showed that a major part of the Arabidopsis and tomato transcriptomic response to sulfate starvation is conserved between these plants and allowed for the identification of processes specifically regulated in tomato at the transcript level, including the control of internal phosphate levels. Integrative gene network analysis uncovered key transcription factors controlling the temporal expression of genes involved in sulfate assimilation, as well as cell cycle, cell division and photosynthesis during sulfate starvation in tomato roots and leaves. Interestingly, one of these transcription factors presents a high identity with SULFUR LIMITATION1, a central component of the sulfate starvation response in Arabidopsis. Conclusions: Together, our results provide the first comprehensive catalog of sulfate-responsive genes in tomato, as well as novel regulatory targets for future functional analyses in tomato and other crops.


2021 ◽  
Author(s):  
◽  
Sarah Elizabeth Burke

<p>This thesis adopted a unique approach to the exploration of proactive socialisation and the processes by which a newcomer moves from organisational outsider to insider. Although socialisation involves actions by the individual, the work group, and the organisation, this study is one of the first to investigate how these actions work in tandem to support the adjustment of organisational newcomers. Research was conducted with a group of 526 participants, drawn from a pool of New Zealand Police (NZ Police) recruits and graduate employees. A quantitative method for data gathering was adopted, with questionnaires administered over a 15-month period for police recruits and 6-month period for graduate newcomers. Results indicated that prior work quality and quantity, job interest, proactive personality, team support, and leader-member exchange each had an important role to play in the prediction of newcomer role breadth self-efficacy. In turn, newcomers who felt confident in their ability to carry out a broader and more proactive role also enjoyed a higher level of task mastery and group fit. The successful achievement of these proximal outcomes led to other, more distal outcomes, namely performance and organisational commitment. Each of these outcomes was achieved, regardless of the socialising tactics employed by the hiring organisation. An important feature of this thesis was the design and delivery of a training intervention that was aimed at coaching newcomers in a range of proactive behaviours (i.e., information-seeking, feedback-seeking, positive framing, relationship building, networking, listening, and observation/modeling). Results found that the longitudinal pattern of proaction differed for newcomers in response to the socialising tactics adopted by the organisation. Results also indicated that the impact of training on future proaction was most potent for individuals who already had an elevated level of role breadth self-efficacy, thereby pointing to the importance of building an employee' perception of their own capability. Training was also most effective when key messages were repeated over multiple sessions, and integrated into the solving of realworld tasks. These results challenge previous studies that have assumed proactivity to be a stable construct over time. Beyond contributing to the literature on newcomer socialisation, this thesis goes some way to clarifying why proactive people actually succeed. It would seem that proactive people expect to be successful, thereby making a training intervention more useful. This thesis also challenges prior research that assumes certain adjustment outcomes are dependent on the socialising tactics adopted by the hiring organisation. Thus, rather than passively adapt to their environment, this research shows how a newcomer can actively shape their own socialising experience. A number of methodological weaknesses found in previous studies have been addressed in this thesis. It also presents a number of practical implications to support the pre-entry, initial entry, and long-term adjustment of seasoned newcomers, versus graduate Generation Y employees. Multiple options for future research are also considered.</p>


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