scholarly journals Extensive recoding of dengue virus type 2 specifically reduces replication in primate cells without gain-of-function in Aedes aegypti mosquitoes

2018 ◽  
Author(s):  
Charles B. Stauft ◽  
Sam H. Shen ◽  
Yutong Song ◽  
Oleksandr Gorbatsevych ◽  
Emmanuel Asare ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Dengue Shock Syndrome ◽  
Great Promise ◽  
Gain Of Function ◽  
Codon Pair Bias ◽  
Life Threatening ◽  
Codon Pair ◽  
Codon Pairs ◽  
And Control

AbstractDengue virus (DENV), an arthropod-borne (“arbovirus”) virus causing a range of human maladies ranging from self-limiting dengue fever to the life-threatening dengue shock syndrome, proliferates well in two different taxa of the Animal Kingdom, mosquitoes and primates. Unexpectedly, mosquitoes and primates have distinct preferences when expressing their genes by translation, e.g. members of these taxa show taxonomic group-specific intolerance to certain codon pairs. This is called “codon pair bias”. By necessity, arboviruses evolved to delicately balance this fundamental difference in their ORFs. Using the mosquito-borne human pathogen DENV we have undone the evolutionarily conserved genomic balance in its ORF sequence and specifically shifted the encoding preference away from primates. However, this recoding of DENV raised concerns of ‘gain-of-function,’ namely whether recoding could inadvertently increase fitness for replication in the arthropod vector. Using mosquito cell cultures and two strains of Aedes aegypti we did not observe any increase in fitness in DENV2 variants codon pair deoptimized for humans. This ability to disrupt and control an arbovirus’s host preference has great promise towards developing the next generation of synthetic vaccines not only for DENV but for other emerging arboviral pathogens such as chikungunya virus and Zika virus.

scholarly journals Desafios para o controle e prevenção do mosquito aedes aegypiti

2017 ◽  
Vol 11 (12) ◽  
pp. 4913 ◽  
Author(s):  
Lays Santos França ◽  
Camilla Massaranduba Alves De Macedo ◽  
Sheylla Nayara Sales Vieira ◽  
Andresa Teixeira Santos ◽  
Gislene De Jesus Cruz Sanches ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Community Health ◽  
Zika Virus ◽  
Public Management ◽  
Prevention And Control ◽  
Structured Interview ◽  
Endemic Diseases ◽  
Virus Zika ◽  
And Control

RESUMOObjetivo: identificar os desafios enfrentados pelos agentes comunitários de saúde e agentes de combate a endemias na prevenção e controle da disseminação do mosquito Aedes aegypti. Método: estudo qualitativo realizado com 12 agentes comunitários de saúde e sete agentes de combate a endemias. Os dados foram produzidos a partir de entrevista semiestruturada e analisados com a técnica do Discurso do Sujeito Coletivo. Resultados: verificou-se que as maiores dificuldades enfrentadas por estes agentes são o descaso, a falta de compromisso e a conscientização da comunidade, além da gestão, que não se apresenta de modo efetivo no processo. Conclusão: propõe-se, assim, uma maior efetivação das ações de educação em saúde junto à população, investimento em educação permanente e sensibilização da gestão pública. Descritores: Prevenção e controle; Participação da Comunidade; Saúde Pública; Vírus da Dengue; Zika Vírus; Vírus Chikungunya.ABSTRACT Objective: to identify the challenges faced by community health agents and agents to combat endemic diseases in the prevention and control of the spread of the Aedes aegypti mosquito. Method: a qualitative study carried out with 12 community health agents and seven agents to combat endemic diseases. The data were produced from a semi-structured interview and analyzed using the Collective Subject Discourse technique. Results: it was verified that the greatest difficulties faced by these agents are the neglect, lack of commitment and awareness of the community, besides the management, that is not presented in an effective way in the process. Conclusion: it is proposed, therefore, a greater effectiveness of the actions of health education with the population, investment in permanent education and public management awareness. Descriptors: Prevention and Control; Community Participation; Public Health; Dengue Virus; Zika Virus; Chikungunya Virus.RESUMEN Objetivo: identificar los desafíos enfrentados por los agentes comunitarios de salud y agentes de combate a endemias en la prevención y control de la diseminación del mosquito Aedes aegypti. Método: estudio cualitativo, realizado con 12 agentes comunitarios de salud y siete agentes de combate a endemias. Los datos fueron producidos a partir de entrevista semiestructurada y analizados con la técnica del Discurso del Sujeto Colectivo. Resultados: se verificó que las mayores dificultades enfrentadas por estos agentes son el descuido, la falta de compromiso y la concientización de la comunidad, además de la gestión, que no se presenta de modo efectivo en el proceso. Conclusión: se propone, así una mayor efectividad de las acciones de educación en salud junto a la población, inversión en educación permanente y sensibilización de la gestión pública. Descriptores: Prevención y Control; Participación de la Comunidad; Salud Pública; Virus del Dengue; Virus Zika; Virus Chikungunya.

scholarly journals A Codon-Pair Bias Associated With Network Interactions in Influenza A, B, and C Genomes

2021 ◽  
Vol 12 ◽  
Author(s):  
Ewan P. Plant ◽  
Zhiping Ye
Keyword(s):  
Influenza Virus ◽  
Rna Structure ◽  
Influenza A ◽  
Protein Translation ◽  
Virus Evolution ◽  
Codon Pair Bias ◽  
Different Types ◽  
Codon Pair ◽  
Codon Pairs ◽  
Insight Into

A new codon-pair bias present in the genomes of different types of influenza virus is described. Codons with fewer network interactions are more frequency paired together than other codon-pairs in influenza A, B, and C genomes. A shared feature among three different influenza types suggests an evolutionary bias. Codon-pair preference can affect both speed of protein translation and RNA structure. This newly identified bias may provide insight into drivers of virus evolution.

Dengue and the introduction of mosquito-transmitted viruses into Australia

2016 ◽  
Vol 37 (4) ◽  
pp. 167
Author(s):  
Andrew F van den Hurk
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Virus Transmission ◽  
Current Situation ◽  
Mosquito Vector ◽  
And Control

Dengue virus outbreaks involving 100s of cases periodically occur in north Queensland, the area of Australia where the primary mosquito vector, Aedes aegypti, occurs. This article summarises the ecology, history, current situation and control of dengue virus transmission in Australia and examines the threat posed by newly emergent arboviruses, such as Zika and chikungunya viruses.

scholarly journals DETEKSI MOLEKULER VIRUS DENGUE SEROTIPE 3 PADA NYAMUK Aedes aegypti DI WILAYAH PURWOKERTO TIMUR

2020 ◽  
Vol 2 (2) ◽  
pp. 215
Author(s):  
Priskila Agnesia Prayitno ◽  
Endang Srimurni Kusmintarsih ◽  
Daniel Joko Wahyono
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Dengue Hemorrhagic Fever ◽  
Sampling Technique ◽  
Hemorrhagic Fever ◽  
The Body ◽  
Survey Method ◽  
Preliminary Information ◽  
Dengue Transmission ◽  
And Control

Dengue Hemorrhagic Fever (DHF) is an infectious disease caused by the Dengue virus and transmitted through by bite of Ae. aegypti. This mosquito is the main vector transmission of DENV with characteristics of the body and limbs are covered with scales silvery white lines. Mosquitoes are widespread in tropical and subtropical regions, and Purwokerto is an endemic of DHF and at the same time found mosquitoes as the vectors. East Purwokerto is the highest region of DHF cases in Banyumas, and the outbreak was happened in Sokanegara on 2016 until cause death. Based on previous research states that the most found Dengue virus in Purwokerto is serotype 3. Therefore, detection molecular of Ae. aegypti need to be carried out in relation to prediction of dengue transmission to obtain preliminary information on the prevention and control of DENV. The purpose of this research is to detects Dengue virus in adult mosquitoes Ae. Aegypti as vector of DENV. The research method used is survey method withcross sectional and purposive sampling technique. The parameter observed is a positivity DENV serotype 3 on mosquitoes. The analysis of the survey data is a positivity DENV of mosquitoes. The results of the research showed that based on entomological survey in East Purwokerto, DENV serotype 3 was not detected in mosquitoes. Key Words :  Aedes aegypti, dengue hemorrhagic fever, DENV

scholarly journals microRNAs, the Link Between Dengue Virus and the Host Genome

2021 ◽  
Vol 12 ◽  
Author(s):  
Yinghua Su ◽  
Ting Lin ◽  
Chun Liu ◽  
Cui Cheng ◽  
Xiao Han ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Clinical Symptoms ◽  
Dengue Shock Syndrome ◽  
Hemorrhagic Fever ◽  
Host Factors ◽  
Signal Molecules ◽  
Transcriptional Level ◽  
Regulate Gene Expression ◽  
Life Threatening ◽  
And Function

Dengue virus (DENV) is a small envelope virus of Flaviviridae that is mainly transmitted by Aedes aegypti and Aedes albopictus. It can cause dengue fever with mild clinical symptoms or even life-threatening dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). At present, there are no specific drugs or mature vaccine products to treat DENV. microRNAs (miRNAs) are a class of important non-coding small molecular RNAs that regulate gene expression at the post-transcriptional level. It is involved in and regulates a series of important life processes, such as growth and development, cell differentiation, cell apoptosis, anti-virus, and anti-tumor. miRNAs also play important roles in interactions between host and viral genome transcriptomes. Host miRNAs can directly target the genome of the virus or regulate host factors to promote or inhibit virus replication. Understanding the expression and function of miRNAs during infection with DENV and the related signal molecules of the miRNA-mediated regulatory network will provide new insights for the development of miRNA-based therapies.

scholarly journals Silent spread of dengue and dengue haemorrhagic fever to Coimbatore and Erode districts in Tamil Nadu, India, 1998: need for effective surveillance to monitor and control the disease

2000 ◽  
Vol 125 (1) ◽  
pp. 195-200 ◽  
Author(s):  
J. SINGH ◽  
N. BALAKRISHNAN ◽  
M. BHARDWAJ ◽  
P. AMUTHADEVI ◽  
E. G. GEORGE ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Surveillance System ◽  
Tamil Nadu ◽  
Dengue Haemorrhagic Fever ◽  
Haemorrhagic Fever ◽  
Igg Antibodies ◽  
And Control ◽  
Healthy Persons ◽  
Monitor And Control

Dengue fever (DF) or dengue haemorrhagic fever (DHF) has not previously been reported in Coimbatore and Erode districts in Tamil Nadu in India. In 1998, 20 hospitalized cases of fever tested positive for dengue virus IgM and/or IgG antibodies. All of them had dengue-compatible illness, and at least four had DHF. Two of them died. Sixteen cases were below 10 years of age. The cases were scattered in 15 distantly located villages and 5 urban localities that had a high Aedes aegypti population. Although the incidence of dengue-like illness has not increased recently, almost 89% (95/107) of samples from healthy persons in the community tested positive for dengue IgG antibodies. The study showed that dengue has been endemic in the area, but was not suspected earlier. A strong laboratory-based surveillance system is essential to monitor and control DF/DHF.

scholarly journals Recoding of the Vesicular Stomatitis Virus L Gene by Computer-Aided Design Provides a Live, Attenuated Vaccine Candidate

mBio ◽  
2015 ◽  
Vol 6 (2) ◽  
Author(s):  
Bingyin Wang ◽  
Chen Yang ◽  
Gergely Tekes ◽  
Steffen Mueller ◽  
Aniko Paul ◽  
...  
Keyword(s):  
Vesicular Stomatitis Virus ◽  
Large Scale ◽  
Vaccine Candidate ◽  
Rna Virus ◽  
Live Attenuated Vaccine ◽  
Live Attenuated Vaccines ◽  
Codon Pair Bias ◽  
Codon Pair ◽  
Vesicular Stomatitis ◽  
Codon Pairs

ABSTRACTCodon pair bias (CPB), which has been observed in all organisms, is a neglected genomic phenomenon that affects gene expression. CPB results from synonymous codons that are paired more or less frequently in ORFeomes regardless of codon bias. The effect of an individual codon pair change is usually small, but when it is amplified by large-scale genome recoding, strikingly altered biological phenotypes are observed. The utility of codon pair bias in the development of live attenuated vaccines was recently demonstrated by recodings of poliovirus (a positive-strand RNA virus) and influenza virus (a negative-strand segmented RNA virus). Here, the L gene of vesicular stomatitis virus (VSV), a nonsegmented negative-sense RNA virus, was partially recoded based on codon pair bias. Totals of 858 and 623 silent mutations were introduced into a 5′-terminal segment of the viral L gene (designated L1) to create sequences containing either overrepresented or underrepresented codon pairs, designated L1sdmaxand L1min, respectively. Analysis revealed that recombinant VSV containing the L1minsequence could not be recovered, whereas the virus with the sdmax sequence showed a modest level of attenuation in cell culture. More strikingly, in mice the L1sdmaxvirus was almost as immunogenic as the parental strain but highly attenuated. Taken together, these results open a new road to attain a balance between VSV virulence and immunogenicity, which could serve as an example for the attenuation of other negative-strand, nonsegmented RNA viruses.IMPORTANCEVesicular stomatitis virus (VSV) is the prototypic rhabdovirus in the orderMononegavirales. A wide range of human pathogens belong to this family. Using a unique computer algorithm and large-scale genome synthesis, we attempted to develop a live attenuated vaccine strain for VSV, which could be used as an antigen delivery platform for humans. Recombinant VSVs with distinct codon pair biases were rationally designed, constructed, and analyzed in both cell culture and an animal model. One such recombinant virus, L1sdmax, contained extra overrepresented codon pairs in its L gene open reading frame (ORF) and showed promise as an effective vaccine candidate because of a favorable balance between virulence and immunogenicity. Our study not only contributes to the understanding of the underlying mechanism of codon pair bias but also may facilitate the development of live attenuated vaccines for other viruses in the orderMononegavirales.

scholarly journals Extensive recoding of dengue virus type 2 specifically reduces replication in primate cells without gain-of-function in Aedes aegypti mosquitoes

PLoS ONE ◽  
2018 ◽  
Vol 13 (9) ◽  
pp. e0198303 ◽  
Author(s):  
Charles B. Stauft ◽  
Sam H. Shen ◽  
Yutong Song ◽  
Oleksandr Gorbatsevych ◽  
Emmanuel Asare ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Virus Type ◽  
Gain Of Function ◽  
Dengue Virus Type 2

Detection of dengue virus in Aedes mosquitoes in Delhi, India

ENTOMON ◽  
2019 ◽  
Vol 44 (3) ◽  
pp. 213-218
Author(s):  
Suresh Chand Kaushik ◽  
Sukhvir Singh ◽  
Purnima Srivastava ◽  
R. Rajendran
Keyword(s):  
Early Intervention ◽  
Early Detection ◽  
Aedes Aegypti ◽  
Dengue Virus ◽  
Infection Rate ◽  
Transmission Season ◽  
Aedes Mosquitoes ◽  
Alternative Approach ◽  
Human Sera ◽  
Minimum Infection Rate

Detection of viruses in human sera particularly in endemic areas is cumbersome and laborious. Therefore, an alternative approach, Immuno-fluorescence assay (IFA) was performed to determine dengue virus (DENV) positivity in mosquitoes. A total of 1055 adult Aedes aegypti female mosquitoes were tested for IFA test against DENV. Minimum infection rate (MIR) for DENV was found higher during August to November 2016 ranging from 10.75 to 20.83. The average yearly MIR was about 6.64. Higher MIR for Ae. aegypti was found in Sarfabad, Noida (12.71) and Khoda Colony, Ghaziabad (11.90). Minimum MIR (4.67) was observed in Sanjay colony (Faridabad). The main contribution of this study resides in the development of a more suitable monitoring system for early detection of viral circulation and to prioritize early intervention in the non-transmission season.

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