scholarly journals DETEKSI MOLEKULER VIRUS DENGUE SEROTIPE 3 PADA NYAMUK Aedes aegypti DI WILAYAH PURWOKERTO TIMUR

2020 ◽  
Vol 2 (2) ◽  
pp. 215
Author(s):  
Priskila Agnesia Prayitno ◽  
Endang Srimurni Kusmintarsih ◽  
Daniel Joko Wahyono
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Dengue Hemorrhagic Fever ◽  
Sampling Technique ◽  
Hemorrhagic Fever ◽  
The Body ◽  
Survey Method ◽  
Preliminary Information ◽  
Dengue Transmission ◽  
And Control

Dengue Hemorrhagic Fever (DHF) is an infectious disease caused by the Dengue virus and transmitted through by bite of Ae. aegypti. This mosquito is the main vector transmission of DENV with characteristics of the body and limbs are covered with scales silvery white lines. Mosquitoes are widespread in tropical and subtropical regions, and Purwokerto is an endemic of DHF and at the same time found mosquitoes as the vectors. East Purwokerto is the highest region of DHF cases in Banyumas, and the outbreak was happened in Sokanegara on 2016 until cause death. Based on previous research states that the most found Dengue virus in Purwokerto is serotype 3. Therefore, detection molecular of Ae. aegypti need to be carried out in relation to prediction of dengue transmission to obtain preliminary information on the prevention and control of DENV. The purpose of this research is to detects Dengue virus in adult mosquitoes Ae. Aegypti as vector of DENV. The research method used is survey method withcross sectional and purposive sampling technique. The parameter observed is a positivity DENV serotype 3 on mosquitoes. The analysis of the survey data is a positivity DENV of mosquitoes. The results of the research showed that based on entomological survey in East Purwokerto, DENV serotype 3 was not detected in mosquitoes. Key Words :  Aedes aegypti, dengue hemorrhagic fever, DENV

scholarly journals Hubungan Jumlah dan Kepadatan Penghuni Rumah serta Keberadaan Nyamuk dengan Frekuensi Menggigit Nyamuk Aedes aegypti Saat Mencari Darah di Kabupaten Cirebon Provinsi Jawa Barat

2010 ◽  
Vol 2 (2) ◽  
pp. 95-98
Author(s):  
Lukman Hakim ◽  
Asep Jajang Kusnandar
Keyword(s):  
Infectious Disease ◽  
Risk Factor ◽  
Aedes Aegypti ◽  
Dengue Virus ◽  
Dengue Hemorrhagic Fever ◽  
Laboratory Tests ◽  
Hemorrhagic Fever ◽  
Mosquito Larvae ◽  
Residential Settings ◽  
Dengue Transmission

Dengue hemorrhagic fever (DHF) is an infectious disease caused by dengue virus with the widest spread in the Asian region. In Indonesia, every year is always an outbreak in some provinces, the largest occurred in 1998 and 2004 with the number of patient mortality by 79 480 people with 800 people. To know the risk factor for dengue transmission in Cirebon District that had a high DHF cases, had been conducted the research with calculating the number and density of humans at the houses and also survey of density of Aedes aegypti pre-adult and adult stages.The research was resulting that the house index (HI) of Ae. aegypti is 58%, while homes found Ae. aegypti adults is 46%, 6 of which house is the house that is not found larvae / pupae of mosquitoes. The laboratory tests found that the frequency of biting Ae. aegypti mosquitoes average 4.5 times per day, at least 2 times and no more than 7 times per day. Number of people bitten by adult mosquitoes average of 3.1 people per day, is at least 2 people and maximum 5 people per day. Based on the statistically test, it is known that there is significant correlation between the number of inhabitants of the house and the presence of mosquito larvae / pupa of Ae. aegypti mosquito with biting frequency, whereas the most dominant variable was the presence of larvae / pupae of mosquitoes. Furthermore, it is advisable to do the cleaning intensification of Aedes breeding places inand outside the home including those hidden. It is also necessary to place residential settings so that the number of occupants per house so less.

Difusi dan Pola Spasial Sebaran Penyakit Demam Berdarah Dengue (DBD) Di Kota Bandar Lampung

2018 ◽  
Vol 1 (1) ◽  
pp. 87-95
Author(s):  
Nurul Qamila ◽  
Agel Vidian Krama
Keyword(s):  
Public Health ◽  
Spatial Analysis ◽  
Aedes Aegypti ◽  
Dengue Virus ◽  
Spatial Pattern ◽  
Dengue Hemorrhagic Fever ◽  
Health Problem ◽  
Public Health Problem ◽  
Hemorrhagic Fever ◽  
Descriptive Method

Dengue hemorrhagic fever (DHF) is a contagious disease caused by the dengue virus and is transmitted by the mosquito Aedes aegypti (Aa.aegypti). The population is still a public health problem that increases the number of sufferers and also widespread, with population and education. This study aims to reveal the spatial pattern and distribution of Dengue Hemorrhagic Fever (DHF) with the spatial pattern and the spread of Dengue Hemorrhagic Fever (DHF) can result in different locations of these allegations. From the map that can be used for the prevention of Dengue Hemorrhagic Fever (DBD) in Bandar Lampung City. This study aims to reveal the spatial pattern and distribution of Dengue Hemorrhagic Fever (DHF) with the descriptive method and spatial pattern of Dengue Hemorrhagic Fever (DHF) can result in different locations of these allegations. From the map that can be used for the prevention of Dengue Hemorrhagic Fever (DBD) in Bandar Lampung City. Keywords: DHF, Spatial Analysis

scholarly journals TRANSMISI TRANSOVARIAL VIRUS DENGUE PADA NYAMUKAEDES SPP.

2013 ◽  
Vol 5 (1) ◽  
Author(s):  
Angle M. H. Sorisi
Keyword(s):  
Dengue Virus ◽  
Vertical Transmission ◽  
Dengue Hemorrhagic Fever ◽  
Virus Transmission ◽  
Hemorrhagic Fever ◽  
Health Problems ◽  
Transovarial Transmission ◽  
Blood Sucking ◽  
Endemic Areas ◽  
And Control

Abstract: Dengue Hemorrhagic Fever (DHF) is one of the most serious health problems in Indonesia which often causes outbreaks with numerous deaths. The disease is transmitted byAedes sp.females. Generally, dengue virus transmission occurs horizontally from human carriers, and the dengue viruses are passed on bytheir vectors through blood sucking activity. After propagation in the mosquito, the viruses are transmitted to human recipients. In addition, there is a vertical transmission (transovarial) of dengue virusesin the ova of Aedes sp.females. The viruses propagate in the ova that undergo  metamorphosis to become larvae, pupae, and imagoes. The transovarial transmission of dengue virusesin its vectors in endemic areas could be a causative key which is responsible for the phenomenon of increasing cases of DHF. Any effort to prevent and control DHF requires a thorough understanding about virDen transmission, including this transovarial transmission in Aedes spfemales. Keywords: DHF, transovarial transmission, Aedes sp.     Abstrak: Penyakit Demam Berdarah Dengue (DBD) merupakan salah satu masalah kesehatan yang semakin serius di Indonesia dan sering menimbulkan suatu Kejadian Luar Biasa (KLB) dengan jumlah kematian tinggi. Penyakit ditularkan melalui Aedes sp.betina. Transmisi virus dengue umumnya terjadi secara horizontal, yaitu dari manusia pembawa virus dengue ke nyamuk vektor Aedes sp. melalui aktivitasnya mengisap darahSetelah mengalami propagasi  dalam  tubuh nyamuk, virus dengue ditularkan ke  manusia penerima. Selain itu, transmisi virus dapat terjadi secara vertikal (transovarial) yaitu virus dengue dalam tubuh nyamuk vektorAedes sp. betinake ovum, kemudian berpropagasi dalam ovum, larva, pupa, dan imago. Transmisi transovarial virus dengueke vektornya di daerah endemik bisa menjadi kunci penyebab yang bertanggung jawab terhadap fenomena peningkatan kasus deman berdarah dengue. Upaya pencegahan dan penanggulangan DBD memerlukan pengetahuan yang matang tentang adanya infeksi transovarial virDen pada nyamuk Aedes sp. Kata kunci : DBD, transmisi transovarial,  Aedes sp.

scholarly journals Proporsi Larva Aedes aegypti dan Aedes albopictus, Hubungannya dengan Kejadian Demam Berdarah Dengue di Daerah Endemik Suburban Kabupaten Sleman, Yogyakarta

2019 ◽  
pp. 163-170
Author(s):  
Bobby Fahmi Muldan Pahlevi ◽  
Tri Wulandari Kesetyaningsih
Keyword(s):  
Aedes Aegypti ◽  
Aedes Albopictus ◽  
Dengue Hemorrhagic Fever ◽  
Urban Areas ◽  
Endemic Area ◽  
Hemorrhagic Fever ◽  
Suburban Area ◽  
Dengue Transmission ◽  
The Relationship

Dengue Hemorrhagic Fever (DHF) is transmitted by Aedes aegypti and Ae. albopictus. Aedes aegypti chooses to live indoors and Ae. albopictus in outdoors. Aedes aegypti has been proven to play a role in dengue transmission in urban areas. It is assumed that suburbs dominated by gardens, so it is possible that Ae. albopictus acts as a vector. The study aimed to analyze the relationship between Ae. albopictus and DHF incidence in suburban area. The study was conducted in Godean, Sleman Regency. 280 ovitrap were placed indoors and outdoors at across seven villages. Each village was represented by one hamlet, so there were 40 ovitrap of each hamlet. The proportion of ovitrap containing Ae. aegypti and Ae. albopictus larvae was calculated. The results showed that Ae. aegypti larvae was more indoors (78.50%) than outdoors (21.45%) in all villages and negatively correlates with DHF (p=0.036; r=-0.776). Aedes albopictus larvae is more commonly found in gardens (80.64%) than in homes (19.36%) and does not correlate with DHF (p = 0.702). It was concluded that in suburban endemic area of ​​Sleman Regency, Ae. aegypti larvae was more indoors and negatively correlated with DHF. Aedes albopictus larva was more in the garden and did not correlate with DHF incidence.

scholarly journals The influence of natural environmental condition towards the existence of vector's larva of dengue hemorrhagic fever: Aedes Aegypti

2017 ◽  
Author(s):  
Susiana Nugraha
Keyword(s):  
Logistic Regression ◽  
Aedes Aegypti ◽  
Dengue Virus ◽  
Dengue Hemorrhagic Fever ◽  
Water Reservoir ◽  
Hemorrhagic Fever ◽  
Morbidity Rate ◽  
Tropical Regions ◽  
Water Replacement ◽  
Dengue Outbreaks

Dengue hemorrhagic fever is a severe and fatal infection that occurs in tropical regions such as Indonesia. In 2014, recorded that dengue morbidity rate was 5.17 per 100,000 inhabitants (approximately 13031 cases) with mortality rate of 0.84% (110 deaths). Demographic and societal changes such as population growth, urbanization, and modern transportation appear to play an important role in the increased incidence and geographical spread of dengue virus. Aedes aegypti, the urban yellow fever mosquito, is also the principal dengue-carrying vector. The Aedes aegypti mosquitoes as a vector of dengue virus normally live and breed in clean water reservoirs that are not directly related to the land such as: bath, bird drinks, water pot, water jars / barrel, cans, old tires, etc. In Indonesia, dengue outbreaks often occur when the seasons change from dry to rain hor vice versa. This study aimed to figure out the influence of natural environment and the existence of the vector’s larva. A logistic regression was performed to ascertain the effects of temperature, humidity, water replacement and the existence of water reservoir on the likelihood of the existence of the vector’s larva . The logistic regression model was statistically significant, p < .005 and the model explained 69% (Nagelkerke R2). This finding shows that the existence of the mosquito’s larva, influenced by temperature, water replacement activity and the existence of water reservoir. Health education about vector control and environmental engineering are necessary to break the chain of mosquito breeding.

DETECTION OF DENGUE TRANSOVARIAL VIRUS FOR AIDES AEGYPTI MOSQUITOES IN ENDEMIC AREAS OF DENGUE HEMORRHAGIC FEVER

2019 ◽  
Vol 3 (9) ◽  
Author(s):  
Suparmin . ◽  
Arif Widyanto ◽  
Ismi Rajiani
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Dengue Hemorrhagic Fever ◽  
Hemorrhagic Fever ◽  
Positive Sample ◽  
Elisa Method ◽  
Central Java ◽  
Vector Population ◽  
The Government ◽  
Central Java Province

Background: The incidence rate (IR) of Dengue Hemorrhagic Fever per 100,000 population in Central Java has increased dramatically in five years. Banyumas Regency is an endemic area of ​​dengue hemorrhagic fever in Central Java Province. The cause of dengue hemorrhagic fever is four dengue viruses known as DEN-1, DEN-2, DEN-3, and DEN-4. The purpose of this research is to detect transovarial dengue virus in Aedes aegypti mosquito in Banyumas Regency. Methods: The study was conducted by installing ovitrap in Mersi Village, East Purwokerto District, and in Pliken Village, Kembaran District, Banyumas Regency. The study was conducted by installing ovitrap in Mersi Village (9 samples) and Pliken Village (3 samples). The eggs obtained are hatched into larvae in the laboratory. The 3rd and 4th instar larvae were examined for the presence of dengue virus using the Elisa method. Result: The results showed that in Mersi Village, there were two positive samples, and in Pliken Village, there was one positive sample containing dengue virus. The presence of the dengue virus found in the hatched Aedes aegypti larvae (F1) indicates that there has been a transovarial dengue virus from the parent mosquito in its offspring. Conclusion: The study concluded that in Banyumas District, a dengue virus transovarial had occurred in Aedes aegypti mosquitoes. The government and the community should increase efforts to eradicate mosquito nests (dengue hemorrhagic fever) to reduce the vector population of dengue hemorrhagic fever so that cases of dengue hemorrhagic fever can be eliminated.    Keyword: Transovarial dengue virus, Aedes aegypti, Dengue Hemorrhagic Fever

scholarly journals Potensi Transmisi Demam Berdarah Dengue Berdasarkan Indeks Entomologi dan Maya Indeks di Tiga Kelurahan Kecamatan Sukajadi Kota Pekanbaru

2020 ◽  
Vol 19 (2) ◽  
pp. 111-118
Author(s):  
Esy Maryanti ◽  
Ismawati Ismawati ◽  
Unique Prissilia ◽  
Ardini Yovy Puteri
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Dengue Hemorrhagic Fever ◽  
Health Problem ◽  
Risk Index ◽  
Geographical Location ◽  
Hemorrhagic Fever ◽  
Cross Sectional ◽  
Transmission Potential ◽  
Single Larva

Latar belakang: Demam berdarah dengue (DBD) merupakan penyakit yang masih menjadi masalah kesehatan di Indonesia yang disebabkan oleh virus dengue dan ditularkan melalui nyamuk Aedes aegypti. Kejadian DBD selalu ada setiap tahun di Pekanbaru dari tahun 2014-2016 terjadi peningkatan kasus, 2017-2018 kasus DBD mulai menurun tetapi diawal tahun 2019 kasus DBD kembali terjadi peningkatan dan sudah ada kematian sehingga memerlukan perhatian yang lebih serius untuk meningkatkan kewaspadaan terhadap penyakit tersebut. Tujuan penelitian ini adalah untuk menganalisis potensi transmisi DBD berdasarkan indeks entomologi dan maya indeks di tiga kelurahan Kecamatan Sukajadi yang merupakan daerah endemis DBD. Metode: Penelitian survei larva Aedes aegypti dengan pendekatan cross sectional yang dilakukan pada bulan Juli 2019 di tiga kelurahan yaitu Kelurahan Kampung Tengah, Kampung Melayu, Kedungsari Kecamatan Sukajadi. Identifikasi larva menggunakan metode single larva method. Hasil: Sebanyak 181 rumah yang disurvey, didapatkan 822 kontainer yang terdiri dari 683 controllable sites dan 139 disposable sites. Angka bebas jentik sebesar 89,5%, container index 3,4%, house index 10,5% dan Breteau index 21%. Status Maya indeks (MI) yang diukur berdasarkan breeding risk index dan hygiene risk index didapatkan sebanyak 55,80% rumah termasuk dalam status sedang dan 15,47% termasuk ke dalam status MI tinggi. Sebaran keberadaan larva Aedes aegypti berdasarkan letak geografis cukup merata di Kelurahan Kampung Melayu dan Kampung Tengah.Simpulan: Berdasarkan hasil indeks entomologi tersebut didapatkan density figure dalam kategori sedang yang artinya wilayah ini mempunyai potensi transmisi sedang untuk kejadian penyakit DBD. ABSTRACT  Title : Transmission Potential of Dengue hemorrhagic fever based on entomology index and maya index in three sub-districts, Sukajadi District, Pekanbaru CityBackground: Dengue hemorrhagic fever (DHF) is a disease still a health problem in Indonesia caused by the dengue virus that is transmitted through Aedes aegypti. The incidence of DHF is always there every year in Pekanbaru form 2014-2016 there was an increase in cases, from 2017-2018 dengue cases began to decline, but in early 2019 dengue cases increased again and there have been deaths so it requires more serious attention.. The objectives of this study was to analyze the potential transmission of DHF based on the entomology index and maya index in three sub-districts of Sukajadi district.Method: The research was a survey of larva Ae.aegypti in July 2019 from 181 houses in the three village of Sukajadi District. Larvae identification using the single larvae method.Result: A total of 181 houses surveyed obtained 822 containers consisting of 683 controllable sites and 139 disposable sites. The larvae free rates is 89.5%, container index 3.4%, house index 10,5% and Breteau index 21%. Maya index is measures based on breeding risk index and hygiene risk index shows that there are 55.8% of houses in the area are classified as moderate and 15.47% are included in high. The distribution of the existence of Ae.aegypti larvae based on the geographical location is quite evenly distributed in Kampung Melayu and Kampung Tengah.Conclusion: It is found that density figure is in the medium, which means that region has moderate transmission potential for the incidence of DHF

scholarly journals Resistance Status of Aedes albopictus (Skuse) on Malathion in Bengkulu City

2018 ◽  
Vol 7 (2) ◽  
pp. 113-119
Author(s):  
Dessy Triana ◽  
Sitti Rahmah Umniyati ◽  
Budi Mulyaningsih
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Aedes Albopictus ◽  
Adult Female ◽  
Dengue Hemorrhagic Fever ◽  
Endemic Area ◽  
Hemorrhagic Fever ◽  
Evaluation Report ◽  
Control Programs ◽  
Diagnostic Dose

Abstract Dengue Hemorrhagic Fever (DHF) is a disease caused by dengue virus and transmitted by Aedes aegypti mosquito as the main vector and Aedes albopictus as secondary vector. In 2016, Bengkulu City was one of 3 cities that experiencing DHF outbreaks in Indonesia. Insecticides malathion have been used since 1990 in bulk in DHF control programs in Bengkulu City and have not had an evaluation report on Aedes sp. vulnerability to malathion. The purpose of this research was to know the resistance status of Ae albopictus mosquitoes from endemic and sporadic areas of DHF in Bengkulu City to malathion. The sample of the study was the Ae. albopictus adult female mosquitoes which collected from endemic and sporadic areas of DHF in Bengkulu City with ovitrap installation. The resistance test was performed by CDC Bottle Bioassay method with malation 96% and 50 μg/ml diagnostic dose of. The results of this study showed 1% mortality on endemic area and 5% on sporadic area. Aedes albopictus of both areas were resistant to malathion.   Keywords: Ae. albopictus, bottle bioassay, malathion.   Abstrak Demam Berdarah Dengue (DBD) merupakan penyakit yang disebabkan oleh virus dengue dan ditularkan oleh nyamuk Aedes aegypti sebagai vektor utama dan Aedes albopictus sebagai vektor sekunder. Pada tahun 2016, Kota Bengkulu merupakan 1 dari 3 kota yang mengalami KLB DBD di Indonesia. Insektisida malation telah digunakan sejak tahun 1990 secara massal dalam program pengendalian DBD di Kota Bengkulu dan belum memiliki laporan evaluasi mengenai kerentanan Aedes sp terhadap malation. Tujuan penelitian untuk mengetahui status kerentanan nyamuk Ae. albopictus dari daerah endemis dan sporadis DBD Kota Bengkulu terhadap malation. Sampel penelitian adalah nyamuk betina dewasa Ae. albopictus yang dikoleksi dari daerah endemik dan sporadis DBD Kota Bengkulu dengan pemasangan ovitrap. Uji kerentanan dilakukan dengan metode CDC Bottle Bioassay dengan malation 96% dan dosis diagnostik 50 µg/ml. Hasil penelitian ini  menunjukkan mortalitas 1% untuk daerah endemis dan 5% untuk daerah sporadis. Aedes albopictus kedua daerah telah resisten terhadap malation.   Kata Kunci: Ae. albopictus, bottle bioassay, malation.

scholarly journals Maya Index dan Kepadatan Larva Aedes aegypti di Kota Ternate, Maluku Utara

2019 ◽  
pp. 133-142
Author(s):  
Amalan Tomia ◽  
Upik Kesumawati Hadi ◽  
Susi Soviana ◽  
Elok Budi Retnani
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Hemorrhagic Fever ◽  
Larval Habitat ◽  
Survey Method ◽  
Risk Category ◽  
Dengue Virus Infection ◽  
Medium Risk ◽  
Single Larva ◽  
Urban Villages

Dengue hemorrhagic fever (DHF) is a contagious disease caused by the dengue virus and transmitted through the bite of Aedes aegypti. Information regarding larval habitat is very important for the control of Ae. aegypti. The studied aims to determine the density of Ae. aegypti larvae and maya index in Ternate City. The research conducted in 20 urban villages in Ternate City for 5 months. Survey method used was single larva and any water reservoirs were found larvae of Aedes spp.  will be taken as a sample. The purpose of this study to measure the density of Ae. aegypti larvae and maya index in Ternate City. The parameters calculated were Container Index (CI), Breteau Index (BI), House Index (HI), Density Figure (DF), and Maya Index. Based on maya index, 1.990 houses in 20 urban villages in Ternate City included in the medium risk category (78.64%) with CI (43.95%), HI (84.99%) and BI (228.91). Density figure in the high category (DF = 8.7). The study concluded that most of households in the area of study still have the potential for transmission of dengue virus infection.

scholarly journals Effectiveness of Soursop Seed Extract (Annona muricata L) as a Natural Repellent on Aedes Aegypti

2019 ◽  
Vol 8 (12) ◽  
pp. 5012-5018
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Dengue Hemorrhagic Fever ◽  
Hemorrhagic Fever ◽  
Seed Extract ◽  
Effective Concentration ◽  
Insect Repellent ◽  
Annona Muricata ◽  
The Public ◽  
Independent Variable

Background: Dengue Hemorrhagic Fever (DHF) is a disease caused by the dengue virus. City Regencies in Indonesia contracted DHF in 2014 amounting to 84.74%, 2015 amounted to 86.77%, and in 2016 amounted to 90.08%. Soursop plants can act as a repellent (insect repellent). The study aimed was to determine the effectiveness of soursop seed extract (Annona muricata L) as a natural repellent against Aedes aegypti mosquitoes. Method: The study was experimental with the independent variable concentration of soursop seed extract (Annona muricata L) and the dependent variable was Aedes aegypti mosquito that perched on the hand which had been sown with soursop seed extract (Annona muricata L). The sample of this study was 500 Aedes aegypti mosquitoes. This study used 5 concentrations and 5 repetitions for 6 hours of observation. The study was conducted on August 6-10 2018 starting at 08.30-13.30. Result: This study indicate that the concentration that concentrations of 20%, 40%, 60%, 80% and 100% have a mean of 75.2%, 90.8%, 93.5%, 95% and 97.7%, respectively. Based on the results of the ANOVA test, the results of the V-watch observation were obtained 61.60%, 85.07%, 89.89%, 92.88%, and 95.42% respectively. Conclusion: From the results obtained, the concentration of effective soursop seed extract is a concentration of 80% and 100% with a protection power of> 90%. The most effective concentration is 100%. It is recommended to the public to use soursop seed extract as a natural repellent to reduce the use of chemicals containing DEET (N, N-diethylmetatoluamide).

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