Altered Cell-Substrate Behavior on Microporous Membranes is a Result of Disruption and Grip

ABSTRACTPorous membranes are ubiquitous in cell co-culture and tissue-on-a-chip studies. These materials are predominantly chosen for their semi-permeable and size exclusion properties to restrict or permit transmigration and cell-cell communication. However, previous studies have shown pore size, spacing and orientation affect cell behavior including extracellular matrix production and migration. The mechanism behind this behavior is not fully understood. In this study, we fabricated micropatterned non-fouling polyethylene glycol (PEG) islands to mimic pores in order to decouple the effect of surface discontinuity from grip provided by pore wall edges. Similar to porous membranes, we found that the PEG islands hindered fibronectin fibrillogenesis with cells on patterned substrates producing shorter fibrils. Additionally, cell migration speed over micropatterned PEG islands was greater than unpatterned controls, suggesting that disruption of cell-substrate interactions by PEG islands promoted a more dynamic and migratory behavior, similarly to cells migrating on microporous membranes. Preferred cellular directionality during migration was nearly identical between substrates with identically patterned PEG islands and micropores, further confirming disruption of cell-substrate interactions as a common mechanism behind the cellular responses on these substrates. Interestingly, cell spreading and the magnitude of migration speed was significantly greater on porous membranes compared to PEG islands with identical feature size and spacing, suggesting pore edges enhanced cellular grip. These results provide a more complete picture on how porous membranes affect cells which are grown on them in an increasing number of cellular barrier and co-culture studies.

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Micropatterned Poly(ethylene glycol) Islands Disrupt Endothelial Cell–Substrate Interactions Differently from Microporous Membranes

ACS Biomaterials Science & Engineering ◽

10.1021/acsbiomaterials.9b01584 ◽

2019 ◽

Vol 6 (2) ◽

pp. 959-968 ◽

Cited By ~ 4

Author(s):

Zahra Allahyari ◽

Shayan Gholizadeh ◽

Henry H. Chung ◽

Luis F. Delgadillo ◽

Thomas R. Gaborski

Keyword(s):

Endothelial Cell ◽

Ethylene Glycol ◽

Microporous Membranes ◽

Poly Ethylene Glycol ◽

Substrate Interactions ◽

Cell Substrate ◽

Poly Ethylene

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Disrupted Surfaces of Porous Membranes Reduce Nuclear YAP Localization through Diminished Cell Spreading and Enhance Adipogenesis

10.1101/2021.01.31.429012 ◽

2021 ◽

Author(s):

Zahra Allahyari ◽

Stephanie M. Casillo ◽

Spencer J. Perry ◽

Ana P. Peredo ◽

Shayan Gholizadeh ◽

...

Keyword(s):

Stromal Cells ◽

Cell Spreading ◽

Porous Membranes ◽

Substrate Interactions ◽

Adipose Derived Stromal Cells ◽

Downstream Effects ◽

Cell Substrate ◽

Cells Cultured

ABSTRACTDownstream effects of cell-substrate interactions due to porous membranes are not fully understood. Consistent with previous studies using other types of discontinuous or disrupted substrates, we show adipose-derived stromal cells cultured on porous membranes have reduced spreading and nuclear YAP localization as well as an increased propensity towards adipogenesis.Abstract Figure

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Production and Analysis of High Resolution Polymer Replicas of Fibrillar Collagen

Microscopy and Microanalysis ◽

10.1017/s1431927600015312 ◽

1999 ◽

Vol 5 (S2) ◽

pp. 398-399

Author(s):

P. Sims ◽

B. Todd ◽

S. Eppell ◽

T. Li ◽

K. Park ◽

...

Keyword(s):

Cellular Response ◽

Physical Nature ◽

Cell Types ◽

Artificial Substrates ◽

Adherent Cell ◽

Fibrillar Collagen ◽

New Materials ◽

Substrate Interactions ◽

Number Of Factors ◽

Cell Substrate

Adherent cells generally construct the immediate substrate upon which they reside. This may occur via synthesis and secretion of new materials and/or by rearrangement and modification of existing substrate. The response of adherent cell types to an existing substrate can be influenced by a number of factors which include both the chemical and physical nature of the substrate. Cell adhesion, proliferation, differentiation and death can all be substrate dependent. Much effort has been directed toward chemical modification of substrates to regulate one or more of the parameters noted above. A significant, but somewhat smaller, degree of attention has been paid to the effects of the topography and microtopography on the cell response to substrate materials. Studies to date strongly suggest the topography is a significant factor in cell-substrate interactions. As noted above, it is most probable that both the chemistry and the structure of a substrate simultaneously influence the cellular response. However we wished to determine, particularly for artificial substrates, the role which microtopography can play in cell-substrate interactions.

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Real-Time Monitoring of Epithelial Cell-Cell and Cell-Substrate Interactions by Infrared Surface Plasmon Spectroscopy

Biophysical Journal ◽

10.1016/j.bpj.2010.10.017 ◽

2010 ◽

Vol 99 (12) ◽

pp. 4028-4036 ◽

Cited By ~ 53

Author(s):

Victor Yashunsky ◽

Vladislav Lirtsman ◽

Michael Golosovsky ◽

Dan Davidov ◽

Benjamin Aroeti

Keyword(s):

Epithelial Cell ◽

Real Time ◽

Surface Plasmon ◽

Real Time Monitoring ◽

Substrate Interactions ◽

Cell Substrate ◽

Surface Plasmon Spectroscopy ◽

Cell Cell

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EFFECTS OF CRYSTAL SIZE AND ORIENTATION OF SUBSTRATES ON CELL ADHESION: IMPLICATION FOR MEDICAL IMPLANTS

International Journal of Modern Physics B ◽

10.1142/s0217979208047936 ◽

2008 ◽

Vol 22 (18n19) ◽

pp. 3069-3081 ◽

Cited By ~ 7

Author(s):

SHAHAB FAGHIHI ◽

HOJATOLLAH VALI ◽

MARYAM TABRIZIAN

Keyword(s):

Cellular Response ◽

High Pressure Torsion ◽

Control Cell ◽

Cell Activity ◽

Titanium Implants ◽

Polycrystalline Materials ◽

Substrate Interactions ◽

Cell Substrate ◽

Substrate Engineering ◽

And Control

The aim of this study is to investigate the effect of atomic structure of polycrystalline materials on cell-substrate interactions. Samples are prepared from rods and sheets of Ti -6 Al -4 V substrates with predominately two distinct crystallographic orientations as well as nano-structured and annealed titanium fabricated through high-pressure torsion and heat treatment processes. The degree of preosteoblast attachment and rate of growth, which are regulated through the activity and interaction of proteins present in the extracellular matrix, are notably increased on the nano-structured titanium and substrate having predominant [Formula: see text] orientation. The improved cell activity is attributed to the nano-structured feature of these substrates consisting of ultra-fine crystals (< 50 nm) and specific atomic order of [Formula: see text] substrate which provide higher degree of surface wettability. These findings demonstrate the advantages of nano-structured titanium over the conventional and coated titanium implants, as both mechanical properties and cellular response are improved. Furthermore, crystal orientation of the substrates can influence cell responses and, therefore, substrate engineering can be used to improve and control cell-substrate interactions.

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Cell–Substrate Interactions

Molecular and Cellular Biomechanics ◽

10.1201/b18093-6 ◽

2015 ◽

pp. 83-98

Author(s):

Rebecca Urbano ◽

Alisa Clyne

Keyword(s):

Substrate Interactions ◽

Cell Substrate

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Very Long-Chain C24:1 Ceramide Is Increased in Serum Extracellular Vesicles with Aging and Can Induce Senescence in Bone-Derived Mesenchymal Stem Cells

Cells ◽

10.3390/cells8010037 ◽

2019 ◽

Vol 8 (1) ◽

pp. 37 ◽

Cited By ~ 21

Author(s):

Andrew Khayrullin ◽

Priyanka Krishnan ◽

Luis Martinez-Nater ◽

Bharati Mendhe ◽

Sadanand Fulzele ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Extracellular Vesicles ◽

Cardiovascular Health ◽

Rhesus Macaques ◽

Cell Communication ◽

Size Exclusion ◽

Target Cells ◽

Serum Samples

Extracellular vesicles (EVs), including exosomes and microvesicles, function in cell-to-cell communication through delivery of proteins, lipids and microRNAs to target cells via endocytosis and membrane fusion. These vesicles are enriched in ceramide, a sphingolipid associated with the promotion of cell senescence and apoptosis. We investigated the ceramide profile of serum exosomes from young (24–40 yrs.) and older (75–90 yrs.) women and young (6–10 yrs.) and older (25–30 yrs.) rhesus macaques to define the role of circulating ceramides in the aging process. EVs were isolated using size-exclusion chromatography. Proteomic analysis was used to validate known exosome markers from Exocarta and nanoparticle tracking analysis used to characterize particle size and concentration. Specific ceramide species were identified with lipidomic analysis. Results show a significant increase in the average amount of C24:1 ceramide in EVs from older women (15.4 pmol/sample) compared to those from younger women (3.8 pmol/sample). Results were similar in non-human primate serum samples with increased amounts of C24:1 ceramide (9.3 pmol/sample) in older monkeys compared to the younger monkeys (1.8 pmol/sample). In vitro studies showed that primary bone-derived mesenchymal stem cells (BMSCs) readily endocytose serum EVs, and serum EVs loaded with C24:1 ceramide can induce BMSC senescence. Elevated ceramide levels have been associated with poor cardiovascular health and memory impairment in older adults. Our data suggest that circulating EVs carrying C24:1 ceramide may contribute directly to cell non-autonomous aging.

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Wavelength-scanning surface plasmon resonance microscopy: A novel tool for real time sensing of cell-substrate interactions

Biosensors and Bioelectronics ◽

10.1016/j.bios.2019.111717 ◽

2019 ◽

Vol 145 ◽

pp. 111717 ◽

Cited By ~ 6

Author(s):

Youjun Zeng ◽

Jie Zhou ◽

Xueliang Wang ◽

Zhiwen Cai ◽

Yonghong Shao

Keyword(s):

Surface Plasmon Resonance ◽

Real Time ◽

Surface Plasmon ◽

Plasmon Resonance ◽

Substrate Interactions ◽

Cell Substrate ◽

Wavelength Scanning ◽

Surface Plasmon Resonance Microscopy

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Effects of Friction Coefficient and Receptor Number on Cell-Substrate Interactions During Migration

ASME 2010 Summer Bioengineering Conference, Parts A and B ◽

10.1115/sbc2010-19323 ◽

2010 ◽

Author(s):

Henry C. Wong ◽

William C. Tang

Keyword(s):

Cell Migration ◽

Cancer Metastasis ◽

Element Model ◽

Biological Tissues ◽

Substrate Interactions ◽

Strain Behavior ◽

Structural Support ◽

Research Areas ◽

Cell Substrate ◽

A Cell

Biological tissues are composed of cells that adhere to the extracellular matrix (ECM) via cell-surface integrin receptors that bind to specific proteins, such as fibronectin, embedded in the matrix. In this manner, the ECM functions as a structural support for the attached cells, and mechanical forces are able to be transmitted from the cell to the ECM and vice versa [1]. Cell migration, a process that is highly dependent on these mechanical interactions, is important for many normal biological processes and diseases that occur in the human body, which include embryonic development, immune response, would healing, and cancer invasion [2]. Though many continuum models of cell migration have been proposed, there is still a need for a model that can be used to quantitatively understand the mechanical factors that can influence the movement of a cell on a substrate. This would be invaluable to the research areas of tissue engineering as well as cancer metastasis. We utilized a finite element model to elucidate the mechanism of cell-substrate interactions for a cell that consistently migrates in a single direction. Our model follows the approach taken by Gracheva and Othmer [2], but we extended their model to describe two-dimensional plane strain behavior.

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