Measurement of reactive species density in the battery-operated, handheld room temperature atmospheric plasma jet for biomedical applications

2013 ◽  
Author(s):  
X. Pei ◽  
J. Liu ◽  
X. Lu
Keyword(s):  
Biomedical Applications ◽  
Room Temperature ◽  
Plasma Jet ◽  
Reactive Species ◽  
Species Density ◽  
Atmospheric Plasma

scholarly journals Tiny Cold Atmospheric Plasma Jet for Biomedical Applications

Processes ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 249
Author(s):  
Zhitong Chen ◽  
Richard Obenchain ◽  
Richard E. Wirz
Keyword(s):  
Biomedical Applications ◽  
Plasma Jet ◽  
Discharge Voltage ◽  
Plasma Jets ◽  
Atmospheric Plasma ◽  
Physical Parameters ◽  
Cold Atmospheric Plasma ◽  
Water Metal ◽  
Plasma Probe ◽  
Jet Nozzle

Conventional plasma jets for biomedical applications tend to have several drawbacks, such as high voltages, high gas delivery, large plasma probe volume, and the formation of discharge within the organ. Therefore, it is challenging to employ these jets inside a living organism’s body. Thus, we developed a single-electrode tiny plasma jet and evaluated its use for clinical biomedical applications. We investigated the effect of voltage input and flow rate on the jet length and studied the physical parameters of the plasma jet, including discharge voltage, average gas and subject temperature, and optical emissions via spectroscopy (OES). The interactions between the tiny plasma jet and five subjects (de-ionized (DI) water, metal, cardboard, pork belly, and pork muscle) were studied at distances of 10 mm and 15 mm from the jet nozzle. The results showed that the tiny plasma jet caused no damage or burning of tissues, and the ROS/RNS (reactive oxygen/nitrogen species) intensity increased when the distance was lowered from 15 mm to 10 mm. These initial observations establish the tiny plasma jet device as a potentially useful tool in clinical biomedical applications.

A single electrode room-temperature plasma jet device for biomedical applications

2008 ◽  
Vol 92 (15) ◽  
pp. 151504 ◽  
Author(s):  
XinPei Lu ◽  
ZhongHe Jiang ◽  
Qing Xiong ◽  
ZhiYuan Tang ◽  
Yuan Pan
Keyword(s):  
Biomedical Applications ◽  
Room Temperature ◽  
Plasma Jet ◽  
Temperature Plasma

scholarly journals Formation of reactive nitrogen species including peroxynitrite in physiological buffer exposed to cold atmospheric plasma

RSC Advances ◽  
2016 ◽  
Vol 6 (82) ◽  
pp. 78457-78467 ◽  
Author(s):  
Fanny Girard ◽  
Vasilica Badets ◽  
Sylvie Blanc ◽  
Kristaq Gazeli ◽  
Laurent Marlin ◽  
...  
Keyword(s):  
Reactive Nitrogen Species ◽  
Biomedical Applications ◽  
Reactive Species ◽  
Atmospheric Plasma ◽  
Reactive Nitrogen ◽  
Nitrogen Species ◽  
Cold Atmospheric Plasma ◽  
Atmospheric Plasmas

Cold Atmospheric Plasmas (CAPs) are increasingly used for biomedical applications, their various reactive components must be then better determined. We demonstrate that peroxynitrite (ONOO−) is effectively a major reactive species generated by CAPs.

scholarly journals The design and manufacture of atmospheric plasma jet surgical handpiece

2020 ◽  
Vol 13 (6) ◽  
pp. 235-240
Author(s):  
Hamed Bagheri ◽  
Reza Reiazi ◽  
Mohammad Kasaie ◽  
Hosein Mootabian
Keyword(s):  
Cancer Cells ◽  
Gas Flow ◽  
Room Temperature ◽  
Low Cost ◽  
Plasma Jet ◽  
Atmospheric Plasma ◽  
Effective Parameters ◽  
Stable Performance ◽  
Design And Manufacture ◽  
Low Cost Manufacturing

AbstractBackgroundAtmospheric plasma jet has different medical applications due to its low temperature at room temperature. In recent years, the effect of nonthermal plasmas on cancer cells has been studied, and it has been shown that this type of plasma has anti-proliferative effects on cancer cells.ObjectivesTo design a plasma jet handpiece, which can be used in cutting operations in less bleeding surgery, eliminating cancer cells without damage to healthy cells and reducing the duration of wound healing.MethodsThe plasma handpiece simply consists of a nozzle body and two cathode and anode electrodes and a fully insulated body against heat and high voltage. Argon is introduced into the handpiece, and by plasma treatment, it is used for special purposes. Each piece was made according to its own manufacturing process and by assembling; the final product of the atmospheric plasma jet handpiece was ready for testing. The jet pipeline was then tested, and the effective parameters were examined.ResultsThe cold atmospheric plasma jet length depends on factors such as power supply, applied voltage, gas flow rate and the distance between the electrodes. The results showed with increasing velocity, the flame and jet lengths decreased greatly due to high losses of plasma, including ions and electrons. Also with increasing the velocity of argon gas, its concentration decreased.ConclusionsIt is concluded that the performance of the proposed design is successful. The advantages include low-cost manufacturing, highly stable performance, and low erosion and can be considered for future development.

scholarly journals Anticancer Effects of Plasma-Activated Medium Produced by a Microwave-Excited Atmospheric Pressure Argon Plasma Jet

2020 ◽  
Vol 2020 ◽  
pp. 1-17
Author(s):  
Ara Jo ◽  
Hea Min Joh ◽  
Tae Hun Chung ◽  
Jin Woong Chung
Keyword(s):  
Flow Cytometry ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Atmospheric Pressure ◽  
A549 Cells ◽  
Plasma Jet ◽  
Reactive Species ◽  
Atmospheric Plasma ◽  
Anticancer Effects ◽  
Indirect Treatment

Cold atmospheric plasma (CAP) has been reported to have strong anticancer effects in vitro and in vivo. CAP has been known to induce apoptosis in most cancer cells by treatment to cells using direct and indirect treatment methods. There are many reports of apoptosis pathways induced by CAP, but for indirect treatment, there is still a lack of fundamental research on how CAP can cause apoptosis in cancer cells. In this study, we applied an indirect treatment method to determine how CAP can induce cancer cell death. First, plasma-activated medium (PAM) was produced by a 2.45 GHz microwave-excited atmospheric pressure plasma jet (ME-APPJ). Next, the amounts of various reactive species in the PAM were estimated using colorimetric methods. The concentration of NO2– and H2O2 in PAM cultured with cancer cells was measured, and intracellular reactive oxidative stress (ROS) changes were observed using flow cytometry. When PAM was incubated with A549 lung cancer cells, there was little change in NO2– concentration, but the concentration of H2O2 gradually decreased after 30 min. While the intracellular ROS of A549 cells was rapidly increased at 2 hours, there was no significant change in that of PAM-treated normal cells. Furthermore, PAM had a significant cytotoxic effect on A549 cells but had little effect on normal cell viability. In addition, using flow cytometry, we confirmed that apoptosis of A549 cells occurred following flow cytometry and western blot analysis. These results suggest that among various reactive species produced by PAM, hydrogen peroxide plays a key role in inducing cancer cell apoptosis.

OPTICAL EMISSION SPECTROSCOPY ANALYSIS OF ATMOSPHERIC PLASMA JET PLUME ON BACTERIA INACTIVATION

2015 ◽  
Vol 77 (6) ◽  
Author(s):  
Nor Aimi Saad ◽  
SK Zaaba ◽  
M.T. Mustaffa ◽  
A. Zakaria ◽  
Roslan Md Nor ◽  
...  
Keyword(s):  
Emission Spectroscopy ◽  
Optical Emission Spectroscopy ◽  
Plasma Plume ◽  
Optical Emission ◽  
Plasma Jet ◽  
Reactive Species ◽  
Microbial Inactivation ◽  
Atmospheric Plasma ◽  
Spectroscopy Analysis ◽  
Bacteria Inactivation

In this paper, an atmospheric plasma jet plasma plume generated using Helium gas was investigated for reactive plasma species. The method of investigation is by using Optical Emission Spectroscopy analysis. Observation of the emission spectrum enables understanding of the influence of reactive species inside plasma plume to microbial inactivation process. The reactive species in plasma plume were detected using spectrometer without presence of bacteria. Escherichia coli and Methicillin-resistant staphylococcus aureus were used as inactivation targets. Bacteria were cultured in 10 Colony Forming Unit per milliliter in single colony and exposed to plasma at different time. It is found that, both bacteria were inactivated at 180 seconds.  The result of emission line spectrum showed the presence of nitrogen and oxygen between line 300 nm until 700 nm. Nitrogen and oxygen are involved in oxidation process which is known as Reactive Nitrogen Species and Reactive Oxygen Species. These species are main key in bacteria inactivation.

scholarly journals Structural and Optical Sensing Properties of Nonthermal Atmospheric Plasma-Synthesized Polyethylene Glycol-Functionalized Gold Nanoparticles

Nanomaterials ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1678
Author(s):  
Linh Nguyen ◽  
Pradeep Lamichhane ◽  
Eun Choi ◽  
Geon Lee
Keyword(s):  
Gold Nanoparticles ◽  
Polyethylene Glycol ◽  
Dye Degradation ◽  
Particle Diameter ◽  
Plasma Jet ◽  
Reactive Species ◽  
Atmospheric Plasma ◽  
Average Particle ◽  
Functionalized Gold Nanoparticles ◽  
Gold Precursor

Polyethylene glycol-functionalized gold nanoparticles (Au@PEG NPs) were prepared by a simple plasma-assisted method without additional reducing chemicals. After irradiating tetrachloroauric acid (HAuCl4) and polyethylene glycol (PEG) in aqueous medium with an argon plasma jet, the gold precursor transformed into an Au@PEG NP colloid that exhibited surface plasma resonance at 530 nm. When the plasma jet entered the water, additional reactive species were induced through interactions between plasma-generated reactive species and aqueous media. Interaction of the gold precursor with the plasma-activated medium allowed the synthesis of gold nanoparticles (AuNPs) without reductants. The plasma-synthesized Au@PEG NPs had a quasi-spherical shape with an average particle diameter of 32.5 nm. The addition of PEG not only helped to stabilize the AuNPs but also increased the number of AuNPs. Au@PEG NP-loaded paper (AuNP-paper) was able to detect the degradation of rhodamine B, therefore, indicating that AuNP-paper can act as a surface-enhanced Raman scattering platform. Dye degradation by plasma treatment was investigated by optical absorption and Raman spectroscopy. The method proposed for the fabrication of Au@PEG NPs is rapid, low-cost, and environment-friendly and will facilitate the application of plasma-synthesized nanomaterials in sensors.

Sign in / Sign up

Export Citation Format

Share Document