scholarly journals The design and manufacture of atmospheric plasma jet surgical handpiece

2020 ◽  
Vol 13 (6) ◽  
pp. 235-240
Author(s):  
Hamed Bagheri ◽  
Reza Reiazi ◽  
Mohammad Kasaie ◽  
Hosein Mootabian
Keyword(s):  
Cancer Cells ◽  
Gas Flow ◽  
Room Temperature ◽  
Low Cost ◽  
Plasma Jet ◽  
Atmospheric Plasma ◽  
Effective Parameters ◽  
Stable Performance ◽  
Design And Manufacture ◽  
Low Cost Manufacturing

AbstractBackgroundAtmospheric plasma jet has different medical applications due to its low temperature at room temperature. In recent years, the effect of nonthermal plasmas on cancer cells has been studied, and it has been shown that this type of plasma has anti-proliferative effects on cancer cells.ObjectivesTo design a plasma jet handpiece, which can be used in cutting operations in less bleeding surgery, eliminating cancer cells without damage to healthy cells and reducing the duration of wound healing.MethodsThe plasma handpiece simply consists of a nozzle body and two cathode and anode electrodes and a fully insulated body against heat and high voltage. Argon is introduced into the handpiece, and by plasma treatment, it is used for special purposes. Each piece was made according to its own manufacturing process and by assembling; the final product of the atmospheric plasma jet handpiece was ready for testing. The jet pipeline was then tested, and the effective parameters were examined.ResultsThe cold atmospheric plasma jet length depends on factors such as power supply, applied voltage, gas flow rate and the distance between the electrodes. The results showed with increasing velocity, the flame and jet lengths decreased greatly due to high losses of plasma, including ions and electrons. Also with increasing the velocity of argon gas, its concentration decreased.ConclusionsIt is concluded that the performance of the proposed design is successful. The advantages include low-cost manufacturing, highly stable performance, and low erosion and can be considered for future development.

scholarly journals Interaction of Cold Atmospheric Argon and Helium Plasma Jets with Bio-Target with Grounded Substrate Beneath

2019 ◽  
Vol 9 (21) ◽  
pp. 4528 ◽  
Author(s):  
Irina Schweigert ◽  
Dmitry Zakrevsky ◽  
Pavel Gugin ◽  
Elena Yelak ◽  
Ekaterina Golubitskaya ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Gas Flow ◽  
Fluid Model ◽  
Plasma Jet ◽  
Plasma Jets ◽  
Atmospheric Plasma ◽  
Maximum Efficiency ◽  
Embryonic Cells ◽  
Plasma Parameters

The cold atmospheric pressure plasma jet interaction with the bio-target is studied in the plasma experiment, 2D fluid model simulations, and with MTT and iCELLigence assays of the viability of cancer cells. It is shown, for the first time, that the use of the grounded substrate under the media with cells considerably amplifies the effect of plasma cancer cell treatment in vitro. Plasma devices with cylindrical and plane geometries generating cold atmospheric plasma jets are developed and tested. The sequence of the streamers which forms the plasma jet is initiated with a voltage of 2.5–6.5 kV applied with the frequency 40 kHz. We suggest using the grounded substrate under the bio-target during the plasma jet treatment of cancer cells. The analysis of the measured plasma spectra and comparison of OH-line intensity for different voltages and gas flow rates allows us to find a range of optimal plasma parameters for the enhanced OH generation. The time-dependent viability is measured for human cell lines, A431 (skin carcinoma), HEK 293 (kidney embryonic cells), and A549 (human lung adenocarcinoma cells) after the plasma jet treatment. The results with cell-based experiments (direct treatment) performed with various plasma jet parameters confirm the maximum efficiency of the treatment with the optimal plasma parameters.

Activation of deionized water by atmospheric plasma jet in helium gas flow

2021 ◽  
Author(s):  
P. Y. Tan ◽  
O. H. Chin ◽  
R. Anpalagan ◽  
Y. T. Lau ◽  
H. C. Lee
Keyword(s):  
Gas Flow ◽  
Plasma Jet ◽  
Deionized Water ◽  
Atmospheric Plasma ◽  
Helium Gas

scholarly journals Parameters Affecting the Antimicrobial Properties of Cold Atmospheric Plasma Jet

2019 ◽  
Vol 8 (11) ◽  
pp. 1930 ◽  
Author(s):  
Bih-Show Lou ◽  
Chih-Ho Lai ◽  
Teng-Ping Chu ◽  
Jang-Hsing Hsieh ◽  
Chun-Ming Chen ◽  
...  
Keyword(s):  
Gas Flow ◽  
Treatment Time ◽  
Plasma Jet ◽  
Atmospheric Plasma ◽  
Gas Flow Rate ◽  
Cold Atmospheric Plasma ◽  
E Coli ◽  
Antimicrobial Efficiency ◽  
Ar Gas ◽  
Sample Distance

Using the Taguchi method to narrow experimental parameters, the antimicrobial efficiency of a cold atmospheric plasma jet (CAPJ) treatment was investigated. An L9 array with four parameters of CAPJ treatments, including the application voltage, CAPJ-sample distance, argon (Ar) gas flow rate, and CAPJ treatment time, were applied to examine the antimicrobial activity against Escherichia coli (E. coli). CAPJ treatment time was found to be the most influential parameter in its antimicrobial ability by evaluation of signal to noise ratios and analysis of variance. 100% bactericidal activity was achieved under the optimal bactericidal activity parameters including the application voltage of 8.5 kV, CAPJ-sample distance of 10 mm, Ar gas flow rate of 500 sccm, and CAPJ treatment time of 300 s, which confirms the efficacy of the Taguchi method in this design. In terms of the mechanism of CAPJ’s antimicrobial ability, the intensity of hydroxyl radical produced by CAPJ positively correlated to its antimicrobial efficiency. The CAPJ antimicrobial efficiency was further evaluated by both DNA double-strand breaks analysis and scanning electron microscopy examination of CAPJ treated bacteria. CAPJ destroyed the cell wall of E. coli and further damaged its DNA structure, thus leading to successful killing of bacteria. This study suggests that optimal conditions of CPAJ can provide effective antimicrobial activity and may be grounds for a novel approach for eradicating bacterial infections.

scholarly journals Mismatch of frequencies of ac voltage and streamers propagation in cold atmospheric plasma jet for typical regimes of cancer cell treatment

2021 ◽  
Vol 2100 (1) ◽  
pp. 012020
Author(s):  
I Schweigert ◽  
A Alexandrov ◽  
D Zakrevsky ◽  
E Milakhina ◽  
E Patrakova ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Cancer Cell ◽  
Plasma Jet ◽  
Plasma Source ◽  
Atmospheric Plasma ◽  
Modes Of Operation ◽  
Cold Atmospheric Plasma ◽  
Mode Of Operation ◽  
Voltage Frequency

Abstract Cold atmospheric plasma (CAP) jet generated by the plasma source at 2-6 kV ac voltages with frequencies of 10-50 kHz demonstrate the different modes of operation. Depending on the voltage frequency and amplitude, some streamers in the plasma jet are short and decay before they approach the treated surface. In this case, the effect on the viability of cancer cells when exposed to CAP jet strongly depends on the mode of operation of the discharge or, in other words, on how many times the streamers hit the bio-target during the treatment. The effect on different modes on cancer cells A549 viability is reported.

scholarly journals Production of reactive species in alginate hydrogels for cold atmospheric plasma-based therapies

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Cédric Labay ◽  
Inès Hamouda ◽  
Francesco Tampieri ◽  
Maria-Pau Ginebra ◽  
Cristina Canal
Keyword(s):  
Plasma Treatment ◽  
Cancer Cells ◽  
Gas Flow ◽  
Isotonic Saline ◽  
Chemical Properties ◽  
Plasma Jets ◽  
The Body ◽  
Atmospheric Plasma ◽  
Fast Diffusion ◽  
Alginate Hydrogels

Abstract In the last years, great advances have been made in therapies based in cold atmospheric plasmas (CAP). CAP generate reactive oxygen and nitrogen species (RONS) which can be transferred to liquids. These CAP activated liquids display the same biological efficacy (i.e. on killing cancer cells) as CAP themselves, opening the door for minimally invasive therapies. However, injection of a liquid in the body results in fast diffusion due to extracellular fluids and blood flow. Therefore, the development of efficient vehicles which allow local confinement and delivery of RONS to the diseased site is a fundamental requirement. In this work, we investigate the generation of RONS (H2O2, NO2−, short-lived RONS) in alginate hydrogels by comparing two atmospheric pressure plasma jets: kINPen and a helium needle, at a range of plasma treatment conditions (time, gas flow, distance to the sample). The physic-chemical properties of the hydrogels remain unchanged by the plasma treatment, while the hydrogel shows several-fold larger capacity for generation of RONS than a typical isotonic saline solution. Part of the RONS are quickly released to a receptor media, so special attention has to be put on the design of hydrogels with in-situ crosslinking. Remarkably, the hydrogels show capacity for sustained release of the RONS. The plasma-treated hydrogels remain fully biocompatible (due the fact that the species generated by plasma are previously washed away), indicating that no cytotoxic modifications have occurred on the polymer. Moreover, the RONS generated in alginate solutions showed cytotoxic potential towards bone cancer cells. These results open the door for the use of hydrogel-based biomaterials in CAP-associated therapies.

scholarly journals Cold Atmospheric Pressure Gliding arc Plasma Jet for Decontamination

2016 ◽  
Vol 24 (3S2) ◽  
pp. 103-108
Author(s):  
Do Hoang Tung ◽  
Bach Sy Minh ◽  
Vu Thi Thom ◽  
Lam Thi Huyen Trang ◽  
Cao Thi Huong ◽  
...  
Keyword(s):  
Uv Radiation ◽  
Atmospheric Pressure ◽  
Gas Flow ◽  
Room Temperature ◽  
Plasma Jet ◽  
Plasma Discharge ◽  
Arc Plasma ◽  
Gliding Arc ◽  
Gliding Arc Plasma ◽  
Ar Gas

A cold atmospheric pressure gliding arc plasma jet hasbeen developed and applied to disinfection. The size of the plasma output is about 6 mm in diameter and 10 mm in length. Ar gas at a flow rate of 10 slm and 25 W plasma power are used. Plasma discharge is produced between the divergent electrodes and the jet appears as an effluence of the gas flow. When a \textnormal{pseudomonas} culture is placed at 8 mm below the torch for 1minute, where the gas is at room temperature, the bacteria within a 16 mm diameter circle are almost completely killed. As the UV radiation is well below the safety regulation, the bacteria are inactivated by the total effect of UV radiation and others like the reactive species and the charged particles.

Investigation on Atmospheric Pressure Plasma Jet of DBD and its Application on Bacteria Inactivation

2015 ◽  
Vol 719-720 ◽  
pp. 221-230
Author(s):  
Tao Liang ◽  
Shi Ying Hou
Keyword(s):  
Atmospheric Pressure ◽  
Gas Flow ◽  
Atmospheric Pressure Plasma ◽  
Plasma Jet ◽  
Atmospheric Plasma ◽  
Flow Mode ◽  
Half Cycle ◽  
Multiple Pulse ◽  
Jet Length ◽  
Bacteria Inactivation

Two kinds of multiple-pulse were reported on atmospheric plasma jet (APPJ) of dielectric barrier discharge (DBD) configurations in this study. Meanwhile, the investigation on electrical characteristics of APPJ in helium and helium/air was carried out with the aim of shedding light on the electrode parameters for dissipated power and jet length. The typical bacteria, E. coil, were employed to study the effect by the two APPJ devices (PJ-1 and PJ-2). For PJ-1, multiple-pulse appears in both sides of every half-cycle, but only in the positive half-cycle for PJ-2. A wider power electrode of PJ-1 and further distance from the nozzle to grounding electrode of PJ-2, whether in ambient helium or helium/air, are all conducive to improvement of dissipated power. With the increase of gas flow rate, the jet length will become longer at laminar flow mode, then get shorter, and a permanent to the end. Generally, the introduction of air leads a better sterilization effect, especially for PJ-1. Keywords: Atmospheric Pressure, multiple-pulse, Electrode parameters, dissipated power, jet length, plasma jet. PACS: 52.80.-s, 52.77.-j

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