Quantitative Analysis of Cardiac Tissue Including Fibroblasts Using Three-Dimensional Confocal Microscopy and Image Reconstruction: Towards a Basis for Electrophysiological Modeling

The reconstruction of a three-dimensional image of a specimen from a set of electron micrographs reduces, under certain assumptions about the imaging process in the microscope, to the mathematical problem of reconstructing a density distribution from a set of its plane projections.In the absence of noise we can formulate a purely geometrical criterion, which, for a general object, fixes the resolution attainable from a given finite number of views in terms of the size of the object. For simplicity we take the ideal case of projections collected by a series of m equally spaced tilts about a single axis.

Download Full-text

Study on erythrocytes by SEM photogrammetry (SEMP) technique

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100161187 ◽

1990 ◽

Vol 48 (3) ◽

pp. 724-725

Author(s):

Tong Wensheng ◽

Lu Lianhuang ◽

Zhang Zhijun

Keyword(s):

Quantitative Analysis ◽

Cell Membrane ◽

Clinical Diagnosis ◽

Human Body ◽

Blood Cells ◽

Three Dimensional ◽

Normal Person ◽

Blood Disease ◽

Computation Technique ◽

Important Basis

This is a combined study of two diffirent branches, photogrammetry and morphology of blood cells. The three dimensional quantitative analysis of erythrocytes using SEMP technique, electron computation technique and photogrammetry theory has made it possible to push the study of mophology of blood cells from LM, TEM, SEM to a higher stage, that of SEM P. A new path has been broken for deeply study of morphology of blood cells.In medical view, the abnormality of the quality and quantity of erythrocytes is one of the important changes of blood disease. It shows the abnormal blood—making function of the human body. Therefore, the study of the change of shape on erythrocytes is the indispensable and important basis of reference in the clinical diagnosis and research of blood disease.The erythrocytes of one normal person, three PNH Patients and one AA patient were used in this experiment. This research determines the following items: Height;Length of two axes (long and short), ratio; Crevice in depth and width of cell membrane; Circumference of erythrocytes; Isoline map of erythrocytes; Section map of erythrocytes.

Download Full-text

Laser Scanning Confocal Microscopy and Three-Dimesnsional Reconstruction of the Mitotic Spindles of Unequally Dividing Embryonic Cells

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100158376 ◽

1990 ◽

Vol 48 (3) ◽

pp. 166-167

Author(s):

J. Holy ◽

G. Schatten

Keyword(s):

Confocal Microscopy ◽

Mitotic Spindle ◽

Laser Scanning ◽

Three Dimensional ◽

Laser Scanning Confocal Microscopy ◽

Two Dimensions ◽

Embryonic Cells ◽

Mitotic Spindles ◽

Cleavage Division ◽

Scanning Confocal Microscopy

One of the classic limitations of light microscopy has been the fact that three dimensional biological events could only be visualized in two dimensions. Recently, this shortcoming has been overcome by combining the technologies of laser scanning confocal microscopy (LSCM) and computer processing of microscopical data by volume rendering methods. We have employed these techniques to examine morphogenetic events characterizing early development of sea urchin embryos. Specifically, the fourth cleavage division was examined because it is at this point that the first morphological signs of cell differentiation appear, manifested in the production of macromeres and micromeres by unequally dividing vegetal blastomeres.The mitotic spindle within vegetal blastomeres undergoing unequal cleavage are highly polarized and develop specialized, flattened asters toward the micromere pole. In order to reconstruct the three-dimensional features of these spindles, both isolated spindles and intact, extracted embryos were fluorescently labeled with antibodies directed against either centrosomes or tubulin.

Download Full-text

Three dimensional deblurring of transmitted-light brightfield micrographs

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100148654 ◽

1993 ◽

Vol 51 ◽

pp. 564-565

Author(s):

Santosh Bhattacharyya

Keyword(s):

Image Reconstruction ◽

Three Dimensional ◽

Transmitted Light ◽

Reconstruction Algorithms ◽

3D Image Reconstruction ◽

Structural Details ◽

Spread Function ◽

Early Testing ◽

Linearized Model ◽

Image Reconstruction Algorithms

Three dimensional microscopic structures play an important role in the understanding of various biological and physiological phenomena. Structural details of neurons, such as the density, caliber and volumes of dendrites, are important in understanding physiological and pathological functioning of nervous systems. Even so, many of the widely used stains in biology and neurophysiology are absorbing stains, such as horseradish peroxidase (HRP), and yet most of the iterative, constrained 3D optical image reconstruction research has concentrated on fluorescence microscopy. It is clear that iterative, constrained 3D image reconstruction methodologies are needed for transmitted light brightfield (TLB) imaging as well. One of the difficulties in doing so, in the past, has been in determining the point spread function of the system.We have been developing several variations of iterative, constrained image reconstruction algorithms for TLB imaging. Some of our early testing with one of them was reported previously. These algorithms are based on a linearized model of TLB imaging.

Download Full-text

A quantitative analysis of cell survival, inflammatory response and remodeling processes in a cardiac tissue graft in vivo

The Thoracic and Cardiovascular Surgeon ◽

10.1055/s-2006-925878 ◽

2006 ◽

Vol 54 (S 1) ◽

Author(s):

T Kofidis ◽

K Müller-Stahl ◽

P Akhyari ◽

B Wachsmann ◽

U Klima ◽

...

Keyword(s):

Quantitative Analysis ◽

Inflammatory Response ◽

Cell Survival ◽

Cardiac Tissue ◽

Tissue Graft

Download Full-text

Polymeric Biomaterials for the Treatment of Cardiac Post-Infarction Injuries

Pharmaceutics ◽

10.3390/pharmaceutics13071038 ◽

2021 ◽

Vol 13 (7) ◽

pp. 1038

Author(s):

Sonia Trombino ◽

Federica Curcio ◽

Roberta Cassano ◽

Manuela Curcio ◽

Giuseppe Cirillo ◽

...

Keyword(s):

Tissue Engineering ◽

Cardiac Tissue ◽

Polymeric Nanoparticles ◽

Three Dimensional ◽

Cardiac Regeneration ◽

Biological Properties ◽

Positive Influence ◽

Regenerative Process ◽

Current Status ◽

Polymeric Biomaterials

Cardiac regeneration aims to reconstruct the heart contractile mass, preventing the organ from a progressive functional deterioration, by delivering pro-regenerative cells, drugs, or growth factors to the site of injury. In recent years, scientific research focused the attention on tissue engineering for the regeneration of cardiac infarct tissue, and biomaterials able to anatomically and physiologically adapt to the heart muscle have been proposed as valuable tools for this purpose, providing the cells with the stimuli necessary to initiate a complete regenerative process. An ideal biomaterial for cardiac tissue regeneration should have a positive influence on the biomechanical, biochemical, and biological properties of tissues and cells; perfectly reflect the morphology and functionality of the native myocardium; and be mechanically stable, with a suitable thickness. Among others, engineered hydrogels, three-dimensional polymeric systems made from synthetic and natural biomaterials, have attracted much interest for cardiac post-infarction therapy. In addition, biocompatible nanosystems, and polymeric nanoparticles in particular, have been explored in preclinical studies as drug delivery and tissue engineering platforms for the treatment of cardiovascular diseases. This review focused on the most employed natural and synthetic biomaterials in cardiac regeneration, paying particular attention to the contribution of Italian research groups in this field, the fabrication techniques, and the current status of the clinical trials.

Download Full-text