Morphological characteristics of the blackspot seabream ( Pagellus bogaraveo ) tongue: A structural and immunohistochemical study

Работа основана на результатах гистологического и иммуногистохимического исследований, выполненных на секционном материале 104 трупов людей (59 мужчин и 45 женщин) молодого и старческого возраста. Проведен сравнительный анализ морфологических характеристик тканей мозолистого тела с использованием окрасок гематоксилином и эозином, методом Ниссля (по Снесареву).При иммуногистохимическом исследовании образцов использовали панель антител к глиальному фибриллярному кислому белку ( GFAP ), белку S -100. Установлено, что к старческому возрасту ткань мозолистого тела характеризуется накоплением микроглиоцитов. С возрастом отмечается разрастание иммунопозитивной к GFAP астроглии. Отмечается отсутствие динамики экспрессии белка S -100 с возрастом. Таким образом, выявленная закономерность возрастной изменчивости цитоархитектоники мозолистого тела представляет интерес в диагностической и лечебной работе, а его морфологическая картина в старческом возрасте может служить эквивалентом анатомической нормы. The work is based on the results of histological and immunohistochemical studies performed on the sectional material of 104 human corpses (59 men and 45 women) of young and old age. A comparative analysis of the morphological characteristics of the corpus callosum tissues using hematoxylin and eosin staining, the Nissl method (by Snesarev), by Van Gieson, by Spielmeyer, by Foot. An immunohistochemical study of the samples used a panel of antibodies to glial ﬁbrillar acid protein ( GFAP ), the S -100 protein. It was found that by senile age, the tissue of the corpus callosum is characterized by the accumulation of glial macrophages. With age, there is a proliferation of GFAP -immunopositive astroglia. There is no dynamics of S -100 protein expression with age. Thus, the revealed regularity of age-related variability of the cytoarchitectonics of the corpus callosum is of interest in diagnostic and therapeutic work, and its morphological picture in old age can serve as an equivalent of the anatomical norm.

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Morphological and immunohistochemical features of cartilage tissue used in reconstructive surgery of the perforations of the tympanic membrane

OTORHINOLARYNGOLOGY 2-3(2) 2019 - OTORHINOLARYNGOLOGY ◽

10.37219/2528-8253-2018-2-58 ◽

2018 ◽

pp. 58-68

Author(s):

Ivan Gornostay ◽

Olga Yudina ◽

Lyudmila Petrova

Keyword(s):

Immunohistochemical Study ◽

Morphological Characteristics ◽

Physiological Solution ◽

Cartilage Tissue ◽

Tissue Samples ◽

Native Cartilage ◽

Relevant Today ◽

Flow Features ◽

Immunohistochemical Studies ◽

First Time

Relevance: The problem of graft choosing to close the eardrum defect in chronic purulent otitis media remains relevant today. In the literature there are few scientific studies that examine the graft nutrition mechanisms. The purpose of this study was to study water flow features in the free lying fragmented cartilage graft used by us in myringoplasty. Materials and methods: The mass of fragmented cartilage autotransplants prepared for tympanoplasty, having the same area, was measured using electronic scales before immersing them in a physiological solution and after immersion. An increase of the transplant mass wasafter wetting it in a physiological solution. Morphological and immunohistochemical studies were carried out in 30 cases grouped into three groups: native cartilage, crushed (fragmented) cartilage and crushed cartilage hydrated by physiological solution. The histological material was 90 tissue samples. The histological preparations were made from all the pieces to assess the morphological characteristics of the cartilage. An immunohistochemical study was also conducted with antibodies to aquaporins (AQP 1, AQP 3 and AQP 8) to identify the localization of endogenous water channels in the auricle cartilage and to identify the role and transport function of aquaporins. Results: It is proved that the nutrition of loosely lying fragmented cartilage improves due to the capillary effect after crushing it, the adsorption of fluid by cartilage tissue increases due to the activation of aquaporins. For the first time, the localization of aquaporin AQP8 in the auricle cartilage was determined.

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Muscle differentiation in blackspot seabream (Pagellus bogaraveo, Brunnich): Histochemical and immunohistochemical study of the fibre types

Tissue and Cell ◽

10.1016/j.tice.2008.05.001 ◽

2008 ◽

Vol 40 (6) ◽

pp. 447-458 ◽

Cited By ~ 13

Author(s):

P. Silva ◽

A.M. Rowlerson ◽

L.M.P. Valente ◽

M. Olmedo ◽

R.A.F. Monteiro ◽

...

Keyword(s):

Immunohistochemical Study ◽

Muscle Differentiation ◽

Fibre Types ◽

Pagellus Bogaraveo

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Cell Wall Ultrastructure in Three Strains of a Cariogenic Streptococcus

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100065730 ◽

1971 ◽

Vol 29 ◽

pp. 338-339

Author(s):

M. J. Kramer ◽

Alan L. Coykendall

Keyword(s):

Cell Wall ◽

Dental Caries ◽

Streptococcus Mutans ◽

Microscopic Study ◽

Electron Microscopic Study ◽

Genetic Heterogeneity ◽

Morphological Characteristics ◽

Electron Microscopic ◽

Dna Reassociation ◽

Wall Ultrastructure

During the almost 50 years since Streptococcus mutans was first suggested as a factor in the etiology of dental caries, a multitude of studies have confirmed the cariogenic potential of this organism. Streptococci have been isolated from human and animal caries on numerous occasions and, with few exceptions, they are not typable by the Lancefield technique but are relatively homogeneous in their biochemical reactions. An analysis of the guanine-cytosine (G-C) composition of the DNA from strains K-1-R, NCTC 10449, and FA-1 by one of us (ALC) revealed significant differences and DNA-DNA reassociation experiments indicated that genetic heterogeneity existed among the three strains. The present electron microscopic study had as its objective the elucidation of any distinguishing morphological characteristics which might further characterize the respective strains.

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Aberrant Type C Virus Production in a Cell Line Derived from Balb/3T3

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100094693 ◽

1972 ◽

Vol 30 ◽

pp. 286-287

Author(s):

N. Savage ◽

A. Hackett

Keyword(s):

Electron Microscopy ◽

Cell Line ◽

Leukemia Virus ◽

Morphological Characteristics ◽

Virus Production ◽

Oncogenic Viruses ◽

Endogenous Virus ◽

Virus Particles ◽

Moloney Leukemia Virus ◽

A Cell

A cell line, UC1-B, which was derived from Balb/3T3 cells, maintains the same morphological characteristics of the non-transformed parental culture, and shows no evidence of spontaneous virus production. Survey by electron microscopy shows that the cell line consists of spindle-shaped cells with no unusual features and no endogenous virus particles.UC1-B cells respond to Moloney leukemia virus (MLV) infection by a change in morphology and growth pattern which is typical of cells transformed by sarcoma virus. Electron microscopy shows that the cells are now variable in shape (rounded, rhomboid, and spindle), and each cell type has some microvilli. Virtually all (90%) of the cells show virus particles developing at the cell surface and within the cytoplasm. Maturing viruses, typical of the oncogenic viruses, are found along with atypical tubular forms in the same cell.

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DNA nick end labeling: a reliable marker for apoptosis?

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100141184 ◽

1995 ◽

Vol 53 ◽

pp. 962-963

Author(s):

Anne F. Bushnell ◽

Sarah Webster ◽

Lynn S. Perlmutter

Keyword(s):

Cell Death ◽

Cultured Cells ◽

Apoptotic Cell ◽

Morphological Characteristics ◽

Detection Methods ◽

Tissue Preparation ◽

Preparation Methods ◽

Dna Laddering ◽

Disease States ◽

Reliable Marker

Apoptosis, or programmed cell death, is an important mechanism in development and in diverse disease states. The morphological characteristics of apoptosis were first identified using the electron microscope. Since then, DNA laddering on agarose gels was found to correlate well with apoptotic cell death in cultured cells of dissimilar origins. Recently numerous DNA nick end labeling methods have been developed in an attempt to visualize, at the light microscopic level, the apoptotic cells responsible for DNA laddering.The present studies were designed to compare various tissue processing techniques and staining methods to assess the occurrence of apoptosis in post mortem tissue from Alzheimer's diseased (AD) and control human brains by DNA nick end labeling methods. Three tissue preparation methods and two commercial DNA nick end labeling kits were evaluated: the Apoptag kit from Oncor and the Biotin-21 dUTP 3' end labeling kit from Clontech. The detection methods of the two kits differed in that the Oncor kit used digoxigenin dUTP and anti-digoxigenin-peroxidase and the Clontech used biotinylated dUTP and avidinperoxidase. Both used 3-3' diaminobenzidine (DAB) for final color development.

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SEM and TEM observations of sperm development in the testis of the freshwater fish Oreochromis mossambicus

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100157838 ◽

1990 ◽

Vol 48 (3) ◽

pp. 58-59

Author(s):

Daryl A. Cornish ◽

George L. Smit

Keyword(s):

Morphological Characteristics ◽

Oreochromis Mossambicus ◽

Breeding Cycle ◽

Limited Information ◽

North West ◽

The North ◽

Sem And Tem ◽

Sperm Development ◽

Ultrastructural Characteristics ◽

The University

Oreochromis mossambicus is currently receiving much attention as a candidater species for aquaculture programs within Southern Africa. This has stimulated interest in its breeding cycle as well as the morphological characteristics of the gonads. Limited information is available on SEM and TEM observations of the male gonads. It is known that the testis of O. mossambicus is a paired, intra-abdominal structure of the lobular type, although further details of its characteristics are not known. Current investigations have shown that spermatids reach full maturity some two months after the female becomes gravid. Throughout the year, the testes contain spermatids at various stages of development although spermiogenesis appears to be maximal during November when spawning occurs. This paper describes the morphological and ultrastructural characteristics of the testes and spermatids.Specimens of this fish were collected at Syferkuil Dam, 8 km north- west of the University of the North over a twelve month period, sacrificed and the testes excised.

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Approaches for TEM imaging of cavitation in toughened nylon

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100153737 ◽

1989 ◽

Vol 47 ◽

pp. 352-353

Author(s):

Barbara A. Wood

Keyword(s):

Morphological Characteristics ◽

Structure Property ◽

Polymer Systems ◽

Selective Staining ◽

Structure Property Relationships ◽

Rubber Toughened ◽

Shear Yield ◽

Controversial Topic ◽

Selection Of ◽

Diamond Knife

A controversial topic in the study of structure-property relationships of toughened polymer systems is the internal cavitation of toughener particles resulting from damage on impact or tensile deformation.Detailed observations of the influence of morphological characteristics such as particle size distribution on deformation mechanisms such as shear yield and cavitation could provide valuable guidance for selection of processing conditions, but TEM observation of damaged zones presents some experimental difficulties.Previously published TEM images of impact fractured toughened nylon show holes but contrast between matrix and toughener is lacking; other systems investigated have clearly shown cavitated impact modifier particles. In rubber toughened nylon, the physical characteristics of cavitated material differ from undamaged material to the extent that sectioning of heavily damaged regions by cryoultramicrotomy with a diamond knife results in sections of greater than optimum thickness (Figure 1). The detailed morphology is obscured despite selective staining of the rubber phase using the ruthenium trichloride route to ruthenium tetroxide.

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