Introduction of an efficient method for placenta decellularization with high potential to preserve ultrastructure and support cell attachment

2022 ◽  
Author(s):  
Roya Sajed ◽  
Amir‐Hassan Zarnani ◽  
Zahra Madjd ◽  
Soheila Arefi ◽  
Mohammad Reza Bolouri ◽  
...  
Keyword(s):  
Efficient Method ◽  
Cell Attachment ◽  
High Potential ◽  
Support Cell

Poly(L-Lactic Acid-Co-Aspartic Acid): Interactive Polymers for Tissue Engineering

1995 ◽  
Vol 394 ◽  
Author(s):  
Jeffrey S. Hrkach ◽  
Jean Ou ◽  
Noah Lotan ◽  
Robert Langer
Keyword(s):  
Tissue Engineering ◽  
Lactic Acid ◽  
Cell Growth ◽  
Aspartic Acid ◽  
Biodegradable Polymers ◽  
Cell Attachment ◽  
Biologically Active ◽  
Functional Sites ◽  
Support Cell ◽  
Enhance Cell Attachment

AbstractOne of the challenges in the field of tissue engineering is the development of optimal materials for use as scaffolds to support cell growth and tissue development. For this purpose, we are developing synthetic, biodegradable polymers with functional sites that provide the opportunity to covalently attach biologically active molecules to the polymers, so they can predictably interact with cells in a favorable manner to enhance cell attachment and growth. The preparation of poly(L-lactic acid-co-aspartic acid) comb-like graft copolymers from poly(L-lactic acid-co-β-benzyl-L-aspartate), and the casting of polymer films by solvent evaporation were carried out.

scholarly journals Engineering of Chitosan-Hydroxyapatite-Magnetite Hierarchical Scaffolds for Guided Bone Growth

Materials ◽  
2019 ◽  
Vol 12 (14) ◽  
pp. 2321 ◽  
Author(s):  
Pistone ◽  
Celesti ◽  
Piperopoulos ◽  
Ashok ◽  
Cembran ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Bone Growth ◽  
Cell Attachment ◽  
X Ray Diffraction ◽  
Culture Studies ◽  
X Ray ◽  
Support Cell ◽  
Chitosan Matrix

Bioabsorbable materials have received increasing attention as innovative systems for the development of osteoconductive biomaterials for bone tissue engineering. In this paper, chitosan-based composites were synthesized adding hydroxyapatite and/or magnetite in a chitosan matrix by in situ precipitation technique. Composites were characterized by optical and electron microscopy, thermogravimetric analyses (TGA), x-ray diffraction (XRD), and in vitro cell culture studies. Hydroxyapatite and magnetite were found to be homogeneously dispersed in the chitosan matrix and the composites showed superior biocompatibility and the ability to support cell attachment and proliferation; in particular, the chitosan/hydroxyapatite/magnetite composite (CS/HA/MGN) demonstrated superior bioactivity with respect to pure chitosan (CS) and to the chitosan/hydroxyapatite (CS/HA) scaffolds

scholarly journals A Conserved NXIP Motif Is Required for Cell Adhesion Properties of the Syndecan-4 Ectodomain

2006 ◽  
Vol 281 (43) ◽  
pp. 32156-32163 ◽  
Author(s):  
James R. Whiteford ◽  
John R. Couchman
Keyword(s):  
Cell Adhesion ◽  
Cell Attachment ◽  
Focal Adhesions ◽  
Cell Types ◽  
Binding Activity ◽  
Cytoskeletal Reorganization ◽  
Cell Binding ◽  
Adhesion Properties ◽  
Support Cell ◽  
Β1 Integrins

Syndecans are cell surface proteoglycans involved in cell adhesion and motility. Syndecan-4 is an important component of focal adhesions and is involved in cytoskeletal reorganization. Previous work has shown that the syndecan-4 ectodomain can support cell attachment. Here, three vertebrate syndecan-4 ectodomains were compared, including that of the zebrafish, and we have demonstrated that the cell binding activity of the syndecan-4 ectodomain is conserved. Cell adhesion to the syndecan-4 ectodomain appears to be a characteristic of mesenchymal cells. Comparison of syndecan-4 ectodomain sequences led to the identification of three conserved regions of sequence, of which the NXIP motif is important for cell binding activity. We have shown that cell adhesion to the syndecan-4 ectodomain involves β1 integrins in several cell types.

scholarly journals Cell Attachment Capacity and Compounds of Fibrin Membranes Isolated from Fresh Frozen Plasma and Cryoprecipitate

Membranes ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 783
Author(s):  
Adél Hinsenkamp ◽  
Kiara Kun ◽  
Fatime Gajnut ◽  
Aliz Majer ◽  
Zsombor Lacza ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cell Attachment ◽  
Human Mesenchymal Stem Cells ◽  
Fresh Frozen Plasma ◽  
Fibrinogen Concentration ◽  
Support Cell ◽  
Significant Difference ◽  
Platelet Concentration ◽  
Fresh Frozen ◽  
Frozen Plasma

Fibrin membranes are widely used in regenerative medicine because they are biocompatible, biodegradable, contain growth factors, and support cell attachment. Most commonly they are produced from serum, but they can also be isolated from activated plasma. To increase the fibrinogen concentration of plasma, cryoprecipitate isolation is a possible solution. In this work, cryoprecipitate was prepared from fresh frozen plasma, isolated by plasmapheresis. The concentration of cellular elements, fibrinogen, total protein, and immunoglobulins among others was measured in different concentrations of cryoprecipitates. After activation with Ca-gluconate, fibrin membranes were produced in different thicknesses, and human mesenchymal stem cells were seeded onto the membranes. They were visualized by live-dead staining and their viability was determined by XTT. The platelet-derived growth factor AB content was quantified by ELISA. Our results showed that fibrinogen and platelet concentration can be multiplied in plasma by cryoprecipitate isolation, which affects the thickness and slightly the growth factor content of the membranes. According to live-dead staining, the thickness of the membranes does not influence cell attachment, and XTT measurement did not reveal a significant difference in cell attachment capacity either; however, a growing trend could be observed in the case of some membranes.

scholarly journals Efficient Method to Determine Microarea with High Potential Demand for Broadband Services

2015 ◽  
Vol 60 ◽  
pp. 1728-1737
Author(s):  
Motoi Iwashita ◽  
Akiya Inoue ◽  
Takeshi Kurosawa ◽  
Ken Nishimatsu
Keyword(s):  
Efficient Method ◽  
High Potential ◽  
Broadband Services ◽  
Potential Demand

scholarly journals Electrospun Polyhydroxybutyrate and Poly(L-lactide-co-ε-caprolactone) Composites as Nanofibrous Scaffolds

2014 ◽  
Vol 2014 ◽  
pp. 1-12 ◽  
Author(s):  
Donraporn Daranarong ◽  
Rodman T. H. Chan ◽  
Nico S. Wanandy ◽  
Robert Molloy ◽  
Winita Punyodom ◽  
...  
Keyword(s):  
Cell Cycle Progression ◽  
Cell Attachment ◽  
Fibre Diameter ◽  
Nerve Tissue ◽  
Mitochondrial Activity ◽  
Cycle Progression ◽  
Support Cell ◽  
Nanofibrous Scaffolds ◽  
Average Fibre ◽  
Fibrous Membranes

Electrospinning can produce nanofibrous scaffolds that mimic the architecture of the extracellular matrix and support cell attachment for tissue engineering applications. In this study, fibrous membranes of polyhydroxybutyrate (PHB) with various loadings of poly(L-lactide-co-ε-caprolactone) (PLCL) were successfully prepared by electrospinning. In comparison to PLCL scaffolds, PLCL blends with PHB exhibited more irregular fibre diameter distributions and higher average fibre diameters but there were no significant differences in pore size. PLCL/PHB scaffolds were more hydrophilic (<120°) with significantly reduced tensile strength (ca. 1 MPa) compared to PLCL scaffolds (150.9±2.8∘and5.8±0.5 MPa). Increasing PLCL loading in PHB/PLCL scaffolds significantly increased the extension at break, (4–6-fold). PLCL/PHB scaffolds supported greater adhesion and proliferation of olfactory ensheathing cells (OECs) than those exhibiting asynchronous growth on culture plates. Mitochondrial activity of cells cultivated on the electrospun blended membranes was enhanced compared to those grown on PLCL and PHB scaffolds (212, 179, and 153%, resp.). Analysis showed that PLCL/PHB nanofibrous membranes promoted cell cycle progression and reduced the onset of necrosis. Thus, electrospun PLCL/PHB composites promoted adhesion and proliferation of OECs when compared to their individual PLCL and PHB components suggesting potential in the repair and engineering of nerve tissue.

scholarly journals Fabrication of Poly(pentaerythritol tetrakis (3-mercaptopropionate)/dipentaerythritol penta-/hexa-acrylate)HIPEs Macroporous Scaffold with Alpha Hydroxyapatite via Photopolymerization for Fibroblast Regeneration

Crystals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 746
Author(s):  
Muhammad Imran Azman ◽  
Nunthawan Kwangsawart ◽  
Jitima Preechawong ◽  
Manit Nithitanakul ◽  
Pornsri Sapsrithong
Keyword(s):  
Maximum Stress ◽  
Cell Attachment ◽  
Promising Result ◽  
Low Cost ◽  
Vital Role ◽  
Compressive Modulus ◽  
Polymerization Reaction ◽  
Porous Scaffolds ◽  
Support Cell ◽  
Biomaterial Application

Synthetic biomaterials that can be structured into porous scaffolds for support cell growth have played a role in developing the field of tissue engineering. This research focused on combination of biodegradable emulsion template along with the assisting of low-cost polymerization reaction. The appendage of ester-based surfactant, Hypermer B246, played a vital role which gave an outstanding dispersion in HIPEs system and degradability. PolyHIPEs were prepared by using domestic ultraviolet light source for producing a multiscale porosity material. The morphology showed a promising result of poly(pentaerythritol tetrakis (3-mercaptopropionate)/dipentaerythritol penta-/hexa-acrylate)HIPEs with varied Hypermer B246 surfactant concentration resulting in the pores size increased in between 51.2 ± 9.8 µm to 131.4 ± 26.32 µm. Cellular moieties of poly(TT/DPEHA) HIPEs were confirmed by using SEM while inclusion of hydroxyapatite were confirmed by SEM, FTIR and EDX-SEM and quantified by thermogravimetric analysis. The maximum stress and compressive modulus of the obtained materials were significantly enhanced with HA up to five percent by weight. Poly(TT/DPEHA)HIPEs with HA showed the ability for the cell attachment and the adhesion/proliferation of the cells, suggested that poly(TT/DPEHA) HIPEs with HA were suitable for biomaterial application.

Morphological Comparison of PLGA/Gelatin Scaffolds Produced by Freeze Casting and Freeze Drying Methods

2013 ◽  
Vol 467 ◽  
pp. 108-111 ◽  
Author(s):  
Ali Zamanian ◽  
Farnaz Ghorbani ◽  
Hanieh Nojehdehian
Keyword(s):  
Freeze Drying ◽  
Structural Integrity ◽  
Cell Attachment ◽  
Drying Methods ◽  
Tissue Formation ◽  
Freeze Casting ◽  
Drying Method ◽  
Morphological Comparison ◽  
Support Cell ◽  
Scanning Electron

In this research, PLGA/Gelatin scaffolds were prepared by both freeze drying and freeze casting methods and their physical, mechanical and morphological observations were evaluated and compared to each other. The pore size and percent of porosity was measure for scaffolds fabricated by both freeze drying and freeze casting techniques. These values were more than 200μm and 95%, respectively. The results of scanning electron microscope (SEM) showed that freeze-cast scaffolds had aligned structures in comparison with freeze drying scaffolds whereas these kinds of microstructure were not seen for samples produced by freeze drying method. The compressive strength for freeze-cast scaffolds (3.2 MPa) was higher than freeze drying samples (2.1 MPa). Although adsorption percentage of freeze cast scaffolds was 650%, this parameter was 450% for freeze drying samples. Based on the obtained results, it seems that the freeze-cast scaffolds are able to support cell attachment, to maintain the required structural integrity and to prevent the pores of the scaffolds from collapsing during neo-tissue formation.

scholarly journals Investigation on the printability of bioink based on alginate-gelatin hydrogel and liquid crystals

2020 ◽  
Vol 9 (4) ◽  
pp. 1718-1725
Author(s):  
Alyaa Idrees Abdulmaged ◽  
Chin Fhong Soon ◽  
Balkis A. Talip ◽  
Sheril Amira Othman ◽  
Gim Pao Lim ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Liquid Crystals ◽  
Flow Rate ◽  
Potential Application ◽  
Cell Attachment ◽  
3D Bioprinting ◽  
Minimum Width ◽  
Gelatin Hydrogel ◽  
Support Cell ◽  
Gelatin Concentration

Bioinks of 3D bioprinting have significant potential application in the field of tissue engineering to support cell attachment and proliferation. In this work, the alginate-gelatin-CELC (AGLC) bioink based on different compositions of alginate-gelatin (AG) hydrogel and cholesteryl ester liquid crystals (CELC) was prepared. Primarily, the alginate-gelatin hydrogel with certain concentration of Gelatin (10-50%w/v) was investigated. The printability of the hydrogel reached a minimum width of 1.8 mm at a flow rate of 1 mL/min when the Gelatin concentration was increased to 50 % w/v (AG1050). Subsequently, the respective polymers with 10% w/v Alginate and50% w/v Gelatin blended with 1%, 5%, 10%, 20%, 40%, and 60% w/v of CELC in the preparation of the alginate-gelatin-CELC bioink was further investigated. The printability of the bioink was examined by micro-extrusion based 3D bioprinter. The printability of the bioink enhanced by 27.8% as compared to AG1050 and reached a minimum width of 1.3 mm at a flow rate of 1 mL/min when the CELC concentration was increased to 40% and 60%. The tested properties of the bioink show that the CELC improve shear-thinning and lipid moieties properties to the composite bioink and hence, enhances its printability.

Efficient in situ gene delivery via PEG diacrylate matrices

2018 ◽  
Vol 6 (12) ◽  
pp. 3241-3250 ◽  
Author(s):  
Paresh Shrimali ◽  
Mathew Peter ◽  
Archana Singh ◽  
Neha Dalal ◽  
Suresh Dakave ◽  
...  
Keyword(s):  
Cell Growth ◽  
Gene Delivery ◽  
Cell Attachment ◽  
Support Cell ◽  
Virus Retention

Cryogels of PEGDA support cell growth, and those of PEGDA–GELMA allow only cell attachment but no virus retention while those of PEGDA–PLL enhance transduction via the colocalization of cells and viruses.

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