The article presents the results of the analysis of the biochemical composition of selected in the competitive variety testing of new varieties and promising lines of spring barley, studied on the experimental field ISA-branch of FGBNU FNAC VIM in 2016-2018. Result of research in different years of water availability among the rooms was allocated a group of high-protein barley with improved feed value: 4 varieties, 2 of which are on the state variety testing, and 2 promising lines. The range of variation of protein content in the grain of the selected samples varied from 13.1 to 14.5 %, lysine – from 0.48 to 0.52 %, weight of 1000 grains-from 48.0 to 53.5 g. According to the data obtained the highest value of protein in grain was observed in krupnosortnogo varieties Zlatoyar (GSI), amounting on average for 14.5 %. The increase was equal to 1.1% in relation to the control variety Yaromir. With an average yield of 6.52 t/ha in the years of research grade Accounting have provided protein per hectare in the amount of 0,94 t/ha. A new variety Sudar´ (GSI) and breeding line 149/4-12 h 788 and 129/1-14 h 907 this indicator has been less productive, however, exceeded the grade Yaromir 0.10 to 0.16 t/ha. Given the higher productivity of new promising varieties and breeding lines, the collection of lysine per unit area they have 12.7, 30.8 per cent was higher than that of the Promej class and 10.6-23.6% more than in the variety Yaromir, which indicates their high biological value. Selected hulless line 1137 h is also an encouraging source of protein and other nutrients of plant origin. The selected lines and new varieties, regardless of weather conditions, were able to ensure the production of high-protein and sources' grain for feed purposes, while maintaining productivity.
