APPLICATIONS OF THE TWO-STAGE IN VITRO DIGESTIBILITY METHOD TO VARIETY TESTING

1963 ◽  
Vol 18 (3) ◽  
pp. 181-189 ◽  
Author(s):  
J. W. Dent
Keyword(s):  
In Vitro Digestibility ◽  
Two Stage ◽  
Variety Testing

A two-stage modification method using 1,4-α-glucan branching enzyme lowers the in vitro digestibility of corn starch

2020 ◽  
Vol 305 ◽  
pp. 125441 ◽  
Author(s):  
Junyan Ren ◽  
Shuangdi Chen ◽  
Caiming Li ◽  
Zhengbiao Gu ◽  
Li Cheng ◽  
...  
Keyword(s):  
Corn Starch ◽  
In Vitro Digestibility ◽  
Branching Enzyme ◽  
Two Stage ◽  
Modification Method

Repeatability of in vitro digestibility assays of forages when using fresh, frozen or freeze-dried cow faeces instead of sheep rumen liquor as sources of micro-organisms

1995 ◽  
Vol 1995 ◽  
pp. 110-110 ◽  
Author(s):  
S Akhter ◽  
E Owen ◽  
M K Theodorou ◽  
S L Tembo ◽  
E R Deaville
Keyword(s):  
Freeze Drying ◽  
Rumen Fluid ◽  
In Vitro Digestibility ◽  
Two Stage ◽  
Freeze Dried ◽  
Fresh Frozen ◽  
Sheep Rumen ◽  
Micro Organisms

Previous studies (El Shaer, Omed and Axford, 1987; Akhter, Owen, Fall, O'Donovan and Theodorou, 1994) with the two-stage in vitro procedure of Tilley and Terry (1963) have shown a high correlation between digestibilities of forages as determined using either sheep rumen liquor, sheep faeces or cow faeces as the microbial inoculum. In the first study of the of the present investigation one objective was to examine the repeatability of these digestibility measurements when made on different occasions. A second objective was to assess whether the correlations between faecal and rumen fluid based inocula could be improved if microorganisms were obtained from pairs rather than individual animals. The objective in the second study using forages of known in vivo digestibility, was to investigate the effect of freezing or freeze-drying of faeces on the repeatability of digestibilities of forages determined in vitro using micro-organisms from cow faeces.

A COMPARISON OF IN VITRO AND IN VIVO DRY MATTER DIGESTTBDLITY TECHNIQUES FOR THE EVALUATION OF FORAGE QUALITY

1987 ◽  
Vol 67 (2) ◽  
pp. 573-576 ◽  
Author(s):  
E. V. VALDES ◽  
G. E. JONES
Keyword(s):  
Key Words ◽  
Dry Matter ◽  
Forage Quality ◽  
In Vitro Digestibility ◽  
Two Stage ◽  
Dry Matter Digestibility ◽  
Whole Plant ◽  
Standard Deviations

A two-stage cellulase-pepsin technique (CS) was compared with the two-stage rumen inoculum-pepsin (IVDMD) method to predict in vivo dry matter digestibility (in vivo DMD) in grasses (n = 30) and legumes (n = 25). Comparisons of CS with IVDMD in whole-plant corn (n = 186) were also conducted. There were significant correlations (P < 0.01) between CS and in vivo DMD and between CS and IVDMD in all forages. The coefficients of determination (r2) and residual standard deviations for the prediction of IVDMD by CS varied between 0.76 and 0.90 and 2.5 and 3.4, respectively. Key words: Digestibility (in vitro), digestibility (in vivo), fungal cellulases, forage

Use of a long-term rumen simulation technique (Rusitec) to provide micro-organisms for in vitro digestibility assays

1991 ◽  
Vol 116 (2) ◽  
pp. 297-301 ◽  
Author(s):  
E. Owen ◽  
M. C. N. Jayasuriya ◽  
R. Hamilton ◽  
M. Lalenta
Keyword(s):  
Artificial Saliva ◽  
Simulation Technique ◽  
In Vitro Digestibility ◽  
Two Stage ◽  
Liquid Effluent ◽  
Dry Matter Digestibility ◽  
Micro Organisms ◽  
Good Agreement

SUMMARYFour experiments involving the two-stage in vitro digestibility technique of Tilley & Terry (1963) were undertaken to investigate whether cow rumen liquor could be replaced with effluent or extract from feed residue containing micro-organisms derived from the long-term rumen simulation technique (Rusitec) of Czerkawski & Breckenridge (1977).In Expts 1 and 2, the dry matter digestibility (DMD) of hay was determined using either cow rumen liquor or Rusitec liquid effluent or extract from feed after 48 h of digestion in a Rusitec apparatus using various volumes of liquor and artificial saliva. Digestibilities determined with rumen liquor were c. 70% (c.v. 1·2%) while those determined with Rusitec effluent or extract were c. 62% (c.v. 1·8%). In treatments common to Expts 1 and 2, Rusitec effluent digestibilities were more repeatable than extract or rumen liquor digestibilities.In Expts 3 and 4, Rusitec effluent was compared with cow rumen liquor for determining DMD of seven forages with DMD ranging from 33 to 70%. For a given feed there was good agreement between experiments in DMD values determined with Rusitec effluent, and for ten paired values there was a high correlation (r2 = 0·976) between DMD determined by the two methods. DMD determined using rumen liquor (y) was accurately predicted by Rusitec liquid effluent digestibility (x) from the equation y = 2·663 + 1·06477.x; R.S.D. 2·433.The results show that Rusitec liquid effluent can be used instead of rumen liquor as a source of micro-organisms for the two-stage in vitro digestibility method. Routine use of Rusitec would obviate the need for using fistulated animals.

scholarly journals Comparison of organic matter digestibility determined by in vivo and in vitro methods

2011 ◽  
Vol 50 (No. 2) ◽  
pp. 47-53 ◽  
Author(s):  
J. Forejtová ◽  
F. Lád ◽  
J. Třináctý ◽  
M. Richter ◽  
L. Gruber ◽  
...  
Keyword(s):  
Organic Matter ◽  
In Vitro Digestibility ◽  
Regression Equations ◽  
Two Stage ◽  
In Vitro Techniques ◽  
In Vitro Methods ◽  
Organic Matter Digestibility

A total of 36 samples of feed &ndash; 19 samples of hays and 17 samples of silages were used for estimation of in vivo and in vitro digestibility. The organic matter digestibility (OMD) was estimated by in vivo and two in vitro techniques (method of Tilley and Terry and two-stage pepsin-cellulase method (Pepcel)). The regression equations were calculated from the results obtained in the experiment. Tilley and Terry method provided consistent results of OMD estimation for both the groups of roughages: in vivo OMD = 14.7 + 0.782 &times; [Tilley and Terry] (n = 18; R<sup>2</sup> = 0.76); in vivo OMD = 36.3 + 0.513 &times; [Tilley and Terry] (n = 16; R<sup>2</sup> = 0.75) for hays and silages, respectively. Different accuracy was found out in Pepcel method: in vivo OMD = 37.0 + 0.478 &times; [Pepcel] (n = 19; R<sup>2</sup> = 0.49); in vivo OMD = 37.8 + 0.484 &times; [Pepcel] (n = 17; R<sup>2</sup> = 0.87) for hays and silages, respectively. &nbsp; &nbsp;

USING A CELLULOLYTIC COMPLEX TO ESTIMATE IN VITRO DIGESTIBILITY OF BIRDSFOOT TREFOIL

1990 ◽  
Vol 70 (2) ◽  
pp. 487-493 ◽  
Author(s):  
D. K. DAVIS ◽  
R. L. McGRAW ◽  
D. A. SLEPER ◽  
P. R. BEUSELINCK
Keyword(s):  
Dry Matter ◽  
Lotus Corniculatus ◽  
Neutral Detergent Fiber ◽  
Birdsfoot Trefoil ◽  
In Vitro Digestibility ◽  
Detergent Solution ◽  
Two Stage ◽  
Fiber Digestion ◽  
Dry Matter Digestibility

Digesting forages with a prepared cellulase solution (PCS) has been shown to be a rapid method of obtaining dry matter digestibility (DMD) estimates in many species. This procedure has not been previously used to obtain DMD estimates of broadleaf birdsfoot trefoil (Lotus corniculatus L.). A broad based population consisting of 19 accessions, representing 11 countries of origin, three germplasm pools, and two domesticated cultivars of birdsfoot trefoil was evaluated. The PCS was produced from culturing Trichoderma reesei Simmons culture number QM9414. Fiber and PCS determinations were compared to an in vitro rumen fermentation (IVDMD) procedure. The fiber components measured were; neutral detergent fiber (NDF), acid detergent fiber (ADF), and hemicellulose (HEMI). The PCS procedures involved digestion using a PCS without any pre- or post-treatment (CS), acid-pepsin digestion followed by a single-stage digestion in PCS (APCS), acid detergent solution (ADS) followed by a two-stage digestion in PCS (ADSCS), and neutral detergent solution (NDS) followed by a two-stage digestion in PCS (NDSCS). All of the fiber and PCS procedures were significantly (P ≤ 0.001) correlated with IVDMD except HEMI. The CS procedure underestimated IVDMD by 199 g kg−1; however, it had the best regression coefficient (b = 0.99), had the second best correlation coefficient (r = 0.86), and required less time than the other PCS procedures. The APCS procedure provided the best estimate of IVDMD. It overestimated the IVDMD mean of birdsfoot trefoil by only 30 g kg−1 and was the most accurate (residual standard deviation (RSD) = 22 g kg−1) approach. The CS procedure is recommended when a ranking of samples is desired and actual values of DMD are not of primary interest.Key words: Fiber digestion, germplasm, in vitro dry matter digestibility, Lotus corniculatus L., Trichodenna reesei Simmons

High-Pressure Homogenization Alters Physicochemical Properties and In Vitro Digestibility of Chinese Yam (Dioscorea Opposita Thunb.) Starch

2018 ◽  
Vol 18 (1) ◽  
pp. 10-15
Author(s):  
Wang Yi-Wei ◽  
He Yong-Zhao ◽  
An Feng-Ping ◽  
Huang Qun ◽  
Zeng Feng ◽  
...  
Keyword(s):  
High Pressure ◽  
Physicochemical Properties ◽  
Starch Content ◽  
In Vitro Digestibility ◽  
High Pressure Homogenization ◽  
Starch Granules ◽  
Cross Polarization ◽  
Chinese Yam ◽  
Crystal Type

In this study, Chinese yam starch-water suspension (8%) were subjected to high-pressure homogenization (HPH) at 100 MPa for increasing cycle numbers, and its effect of on the physicochemical properties of the starch was investigated. Results of the polarizing microscope observations showed that the starch granules were disrupted (i.e. greater breakdown value) after HPH treatment, followed by a decrease in cross polarization. After three HPH cycles, the crystallinity of starch decreased, while the crystal type remained unaltered. Meanwhile, the contents of rapidly digestible starch and slowly digestible starch were increased. On the contrary, resistant starch content was decreased. Our results indicate that HPH treatment resulted in reduction of starch crystallinity and increase of starch digestibility.

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