THE INFLUENCE OF FORMALIN AND PARAFFIN EMBEDDING ON THE IMMUNOHISTOCHEMICAL REACTION OF MONOCLONAL ANTIBODIES APPLIED TO FEMALE BREAST TISSUE

2009 ◽  
Vol 92A (1-6) ◽  
pp. 167-175
Author(s):  
B. B. RASMUSSEN ◽  
J. HILGERS ◽  
J. HILKENS
Keyword(s):  
Monoclonal Antibodies ◽  
Breast Tissue ◽  
Paraffin Embedding ◽  
Immunohistochemical Reaction ◽  
Female Breast

scholarly journals Granulomatous Mastitis: A Rare Cause of Male Breast Lump

2016 ◽  
Vol 9 (2) ◽  
pp. 516-519 ◽  
Author(s):  
Abdel Rahman A. Al Manasra ◽  
Mohammad F. Al-Hurani
Keyword(s):  
Breast Tissue ◽  
Nipple Discharge ◽  
Surgical Excision ◽  
Male Breast ◽  
Granulomatous Mastitis ◽  
Idiopathic Granulomatous Mastitis ◽  
Radiologic Findings ◽  
Female Breast ◽  
The Right ◽  
Histopathologic Findings

Background: Mastitis is a common benign disorder of the female breast. It is frequently associated with tenderness, swelling and nipple discharge. We are describing an extremely rare case of an idiopathic granulomatous mastitis in the male breast. Only 1 previous case was reported. Case Report: A 29-year-old male patient presented with a hard, painless lump in the right breast of 2 weeks duration. The patient underwent surgical excision with margin. The histopathologic findings were consistent with granulomatous mastitis. The case was reported as idiopathic granulomatous mastitis after exclusion of all known causes of the disease. Conclusion: Granulomatous mastitis is rare in females and extremely rare in male breast tissue. Since this disease mimics breast cancer in its clinical picture and radiologic findings are usually not conclusive, surgical excision is recommended in all cases.

In vitro optical characterization and discrimination of female breast tissue during near infrared femtosecond laser pulses propagation

2001 ◽  
Vol 6 (4) ◽  
pp. 446 ◽  
Author(s):  
G. Zacharakis ◽  
A. Zolindaki ◽  
V. Sakkalis ◽  
G. Filippidis ◽  
T. G. Papazoglou ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Breast Tissue ◽  
Near Infrared ◽  
Laser Pulses ◽  
Optical Characterization ◽  
Femtosecond Laser Pulses ◽  
Female Breast

scholarly journals Effects of fixation and paraffin embedding on the immunohistological detection of cell-associated HIV-1 by different monoclonal antibodies.

1995 ◽  
Vol 43 (9) ◽  
pp. 857-862 ◽  
Author(s):  
J Pudney ◽  
D Anderson
Keyword(s):  
Monoclonal Antibodies ◽  
Reverse Transcriptase ◽  
Retrospective Analysis ◽  
Glutaraldehyde Fixation ◽  
Paraffin Embedding ◽  
Antigenic Sites ◽  
Wide Range ◽  
Pathological Material ◽  
Hiv 1

This study evaluates a panel of monoclonal antibodies (MAbs) to HIV-1 antigens (DuPont anti-gp120, gp41, p24; Olympus anti-gp120/160, gp41, p24A, p24B, p55, p18A, p18B, reverse transcriptase) for their ability to detect the virus in tissues after exposure to various fixatives (100% acetone, 10% formaldehyde, 2.5% glutaraldehyde, 4% paraformaldehyde/1% glutaraldehyde, Bouin's fluid) and after paraffin embedding. Acetone, 10% formaldehyde, and Bouin's fluid all preserved a wide range of viral epitopes compared with other fixatives. The most robust MAbs were DuPont p24 and Olympus p55, which produced excellent staining regardless of the fixative used. Embedding in paraffin variability influenced the capacity of MAbs to detect HIV-1 epitopes on fixed cells. Certain antibodies (e.g., DuPont gp24, Olympus p24B) produced good staining, whereas other epitopes (e.g., DuPont gp120, formaldehyde) were destroyed. In some cases, paraffin embedding revealed antigenic sites that had been formerly masked (e.g., Olympus gp120 and p24A; formaldehyde and glutaraldehyde fixation). These results indicate that HIV-1 antigens can be detected by immunohistology on cells exposed to most common fixatives. Therefore, retrospective analysis of pathological material is possible, provided that the antibodies are matched to the fixative used to preserve the tissue.

scholarly journals Arsenic Induced Breast Cancer Risk in Population of Bihar, India

2021 ◽  
Author(s):  
Arun Kumar ◽  
Mohammad Ali ◽  
Pintoo Kumar Niraj ◽  
Vivek Akhouri ◽  
Vikas Kumar ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Patients ◽  
Biological Samples ◽  
Breast Tissue ◽  
Arsenic Concentration ◽  
Arsenic Contamination ◽  
The State ◽  
Control Group ◽  
Breast Cancer Patients ◽  
Female Breast

Abstract Globally, ~ 300 million people are exposed to arsenic poisoning while in India an estimated 70 million people are affected by consumption of arsenic contaminated water. The state of Bihar is an endemic belt for arsenic contamination in groundwater affecting over 10 million people with moderate to serious health manifestations. Life threatening disease like cancer is not uncommon now, and carcinoma type of cancer cases are on the rise. Breast cancer incidences in the state follows closely with more and more women getting affected. Approximately, 23% of the cancer types in women are related to breast cancer, diagnosed at a fairly advanced stage (III or IV) of the disease. The etiology of the disease is not clearly known though faulty lifestyle and genetic makeup cannot be ruled out. The present study attempts to derive a relation between increasing breast cancer cases with the sustained arsenic intake in the second most populated state of the country. Pathologically confirmed, female breast cancer patients (n = 55) from across the state were included in the study. Sampling of the breast tissue, blood, hair and toe nail was carried out by the surgical oncology department of the institute. As a part of cross-sectional study, (n = 12) female breast benign cases were taken as the control group and their biological samples were also collected. All the samples as per the protocol of NIOSH were digested and analysed by graphite furnace atomic absorption spectrophotometer. For the epidemiological parameter study, their age, type of malignancy, stage and demographic information was compiled. The results were correlated with the arsenic concentration in groundwater as per their endemic status and anomalous values on GIS platform. The role of geological studies to establish the morpho-stratigraphic control and aquifers with higher concentration was brought to use. The results are fairly indicative of the high correlation of anomalous concentration of arsenic with the sample population with diagnosed malignancy as compared to the control group. The maximum arsenic concentration observed in the biological samples in blood was 1856µg/L, in breast tissue 446.4µg/Kg, in hair 1296·9µg/Kg and in toenail 621·83µg/Kg respectively. The scattered plots correlate the relationship between age of the breast cancer patients with arsenic contamination, while the geospatial distribution positively correlates with the districts with increased arsenic endemicity in a predominantly alluvium dominated country. The high arsenic contamination in the biological samples of the breast cancer patients is an indicative marker to the possible relation of the disease to arsenic, as compared to the control population exposed to a much lesser toxicity. Consumption of water with more than 10 ppb arsenic contamination over a sustained longer time span has possibly exposed the population to a larger threat to disease as inferred from the findings. The disease breeding as a silent killer with reduced or unnoticeable symptoms upto a fairly advanced stage has further accentuated the problem. The present study thus endeavours to identify a significant relation of the disease with sustained intake of arsenic consumed through water and food products laden with anomalous concentration.

Nonlinear imaging of female breast tissue biopsies

2019 ◽  
Author(s):  
Evangelia Gavgiotaki ◽  
Vassilis Tsafas ◽  
Savvas Bovasianos ◽  
Sofia Agelaki ◽  
Vassilis Georgoulias ◽  
...  
Keyword(s):  
Breast Tissue ◽  
Female Breast ◽  
Nonlinear Imaging

In vitro Optical Characterization of Female Breast Tissue with Near Infrared fsec Laser Pulses

2000 ◽  
pp. 294-296
Author(s):  
G. Zacharakis ◽  
V. Sakkalis ◽  
G. Filippidis ◽  
A. Zolindaki ◽  
E. Koumantakis ◽  
...  
Keyword(s):  
Breast Tissue ◽  
Near Infrared ◽  
Laser Pulses ◽  
Optical Characterization ◽  
Female Breast

scholarly journals Presence of Luteinizing Hormone/Human Chorionic Gonadotropin Receptors in Male Breast Tissues

2004 ◽  
Vol 89 (8) ◽  
pp. 4119-4123 ◽  
Author(s):  
Harold E. Carlson ◽  
Philip Kane ◽  
Z. M. Lei ◽  
X. Li ◽  
C. V. Rao
Keyword(s):  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Breast Tissue ◽  
Male Breast ◽  
Normal Male ◽  
Receptor Mrna ◽  
Female Breast ◽  
Breast Tissues ◽  
Hcg Receptor

Receptors for LH/human chorionic gonadotropin (hCG) have been found in a variety of nongonadal tissues including the female breast. Using in situ hybridization and immunohistochemistry, we demonstrated the presence of LH/hCG receptor mRNA and protein in normal male breast tissue obtained at autopsy (n = 4) and archival samples of benign gynecomastia (n = 14) and male breast carcinoma (n = 5). Although the function of these receptors remains to be determined, the findings suggest the possibility that LH and hCG may play a role in the pathogenesis of male breast disorders.

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