Ameliorative effect of hesperidin on streptozotocin‐diabetes mellitus‐induced testicular DNA damage and sperm quality degradation in Sprague–Dawley rats

2021 ◽  
Vol 45 (10) ◽  
Author(s):  
Emrah Hicazi Aksu ◽  
Fatih Mehmet Kandemir ◽  
Sefa Küçükler
Keyword(s):  
Diabetes Mellitus ◽  
Dna Damage ◽  
Sperm Quality ◽  
Streptozotocin Diabetes ◽  
Sprague Dawley ◽  
Quality Degradation ◽  
Sprague Dawley Rats ◽  
Ameliorative Effect

KELULUT HONEY SUPPLEMENTATION PREVENTS SPERM AND TESTICULAR OXIDATIVE DAMAGE IN STREPTOZOTOCIN-INDUCED DIABETIC RATS

2017 ◽  
Vol 79 (3) ◽  
Author(s):  
Siti Balkis Budin ◽  
Fatin Farhana Jubaidi ◽  
Siti Nur Farahana Mohd Noor Azam ◽  
Nur Liyana Mohamed Yusof ◽  
Izatus Shima Taib ◽  
...  
Keyword(s):  
Oxidative Damage ◽  
Sperm Quality ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Sprague Dawley ◽  
Control Groups ◽  
Sod Activity ◽  
Male Adult ◽  
Pathological Conditions ◽  
Sprague Dawley Rats

Previous studies found that Kelulut Honey produced by Trigona spp. bees is able to prevent oxidative damage in various pathological conditions.  Thus, the present study aimed to determine whether Kelulut Honey could prevent the sperm and testicular damage in streptozotocin-induced diabetic rats. Male Adult male Sprague-Dawley rats were divided into four groups: Non-Diabetic (NDM), Non-Diabetic with Kelulut Honey supplementation (NDMKH), Diabetic without supplementation (DM) and Diabetic with Kelulut Honey supplementation (DMKH).  Kelulut honey was given at the dose of 2.0 g/kg weight daily via gavage for 28 consecutive days. Results showed that sperm quality produced by diabetic rats supplemented with Kelulut honey significantly improved compared to the diabetic control groups (p<0.05). SOD activity and GSH level increased significantly (p<0.05) whereas PC and MDA levels significantly decreased in sperm and testis of DMKH rats when compared to DM rats (p<0.05). Histological observation showed obvious increase in spermatozoa in the lumen of epididymis and increased spermatogenic cells density in the testis of DMKH group.  In conclusion, Kelulut Honey has a potential in preventing the damage of sperm and testis in diabetic rats.

scholarly journals Histomorphology, Sperm Quality and Hormonal Profile in Adult Male Sprague-Dawley Rats following administration of aqueous crude Extract of Solanum nigrum by gastric gavage

2018 ◽  
Author(s):  
Sunday Aderemi Adelakun ◽  
Babatunde Ogunlade ◽  
Toluwase Solomon Olawuyi ◽  
Julius Akomaye Aniah ◽  
Olusegun Dare Omotoso
Keyword(s):  
Adult Male ◽  
Crude Extract ◽  
Sperm Quality ◽  
Solanum Nigrum ◽  
Sprague Dawley ◽  
Hormonal Profile ◽  
Sprague Dawley Rats

Juxtamedullary microvascular dysfunction during the hyperfiltration stage of diabetes mellitus

1994 ◽  
Vol 267 (1) ◽  
pp. F99-F105 ◽  
Author(s):  
K. Ohishi ◽  
M. I. Okwueze ◽  
R. C. Vari ◽  
P. K. Carmines
Keyword(s):  
Diabetes Mellitus ◽  
Microvascular Dysfunction ◽  
Sprague Dawley ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Sprague Dawley Rats ◽  
Vasoconstrictor Response ◽  
Wide Range ◽  
Single Nephron

This study was designed to identify and localize defects in renal microvascular function during the hyperfiltration stage of diabetes mellitus. Male Sprague-Dawley rats were injected intravenously with 65 mg/kg streptozotocin (IDDM rats) or vehicle (sham rats). IDDM rats received insulin (3 U.kg-1.day-1) via an osmotic minipump; sham rats received diluent. During the ensuing 2-wk period, blood glucose levels averaged 89 +/- 2 mg/dl in 33 sham rats and 290 +/- 13 mg/dl in 37 IDDM rats. At the end of this period, inulin clearance was elevated in eight IDDM rats (1.43 +/- 0.17 ml.min-1.g kidney wt-1) compared with six sham rats (0.78 +/- 0.05 ml.min-1.g kidney wt-1). The remaining animals served as tissue donors for study of the renal microvasculature using the in vitro blood-perfused juxtamedullary nephron technique. Kidneys from sham and IDDM rats were perfused with homologous blood at a renal arterial pressure of 110 mmHg. Juxtamedullary single-nephron glomerular filtration rate was higher in IDDM rats (41.5 +/- 5.4 nl/min) than in sham rats (25.4 +/- 2.4 nl/min). Afferent arteriolar inside diameter was greater in IDDM rats (34 +/- 2 microns) than in sham rats (22 +/- 1 microns); however, efferent arteriolar diameter did not differ between groups. The afferent arteriolar vasoconstrictor response to norepinephrine (NE) was attenuated in IDDM rats, relative to sham rats, over a wide range of NE concentrations. In contrast, NE evoked similar degrees of efferent vasoconstriction in IDDM and sham rats.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Acrylonitrile-Induced Oxidative Stress and Oxidative DNA Damage in Male Sprague-Dawley Rats

2009 ◽  
Vol 111 (1) ◽  
pp. 64-71 ◽  
Author(s):  
Xinzhu Pu ◽  
Lisa M. Kamendulis ◽  
James E. Klaunig
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Oxidative Dna Damage ◽  
Sprague Dawley ◽  
Sprague Dawley Rats

scholarly journals The ameliorative effect of black soybean and purple sweet potato to improve sperm quality through suppressing reactive oxygen species (ROS) in type 2 diabetes mellitus rat (Rattus novergicus)

ScienceAsia ◽  
2018 ◽  
Vol 44 (5) ◽  
pp. 303
Author(s):  
Abdul Gofur ◽  
Agung Witjoro ◽  
Erni Widya Ningtiyas ◽  
Evi Setyowati ◽  
Siti Aminatul Mukharromah ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Reactive Oxygen Species ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Sperm Quality ◽  
Oxygen Species ◽  
Black Soybean ◽  
Ameliorative Effect ◽  
Purple Sweet Potato

Protective effect of alpha glucosyl hesperidin (G-hesperidin) on chronic vanadium induced testicular toxicity and sperm nuclear DNA damage in male Sprague Dawley rats

Andrologia ◽  
2014 ◽  
Vol 47 (5) ◽  
pp. 568-578 ◽  
Author(s):  
B. Vijaya Bharathi ◽  
G. Jaya Prakash ◽  
K. M. Krishna ◽  
C. H. Ravi Krishna ◽  
T. Sivanarayana ◽  
...  
Keyword(s):  
Dna Damage ◽  
Protective Effect ◽  
Nuclear Dna ◽  
Testicular Toxicity ◽  
Sprague Dawley ◽  
Sprague Dawley Rats

Preclinical Study to Evaluate the Effect of Polyherbal Formulation on Metformin: Potential Herb-drug Interaction

2019 ◽  
Vol 9 (1) ◽  
pp. 69-76
Author(s):  
Devendra Kumar ◽  
Neerja Trivedi ◽  
Rakesh Kumar Dixit
Keyword(s):  
Diabetes Mellitus ◽  
Preclinical Study ◽  
Oral Route ◽  
Diabetic Rats ◽  
Pharmacokinetic Parameters ◽  
Sprague Dawley ◽  
Pharmacokinetics And Pharmacodynamics ◽  
Polyherbal Formulation ◽  
Sprague Dawley Rats ◽  
Significant Difference

Objective: The aim of the present study was to examine the effect of a polyherbal formulation (PHF) on pharmacokinetics and pharmacodynamics of metformin in rats. Methods: The present study was conducted to determine the beneficial outcomes of PHF along with metformin by studying herb-drug interactions. PHF was prepared by five indigenous herbs, Those are being used traditionally as antidiabetic in India. PHF doses (100 mg/kg/day) were administered to Sprague-Dawley rats by an oral route of different groups for multiple weeks except for control. Metformin (100 mg/kg) was orally administered at 7th and 30th day to control and PHF pretreated rats for pharmacokinetics study while pharmacodynamics study was conducted in PHF treated and untreated diabetic rats. No more significant difference was found in the pharmacokinetic parameters in PHF treated at 7th day while a significant increase was found in AUC at the 30th day. Results: The hypoglycemic effect was observed with a combination of metformin and PHF, significantly more compared to control. Metformin decreased the blood glucose 1.51 fold at 7th day and 1.7 fold at 30th day respectively, compared to control. Conclusion: Thus, this finding indicates that PHF increased the AUC of metformin. It might increase bioavailability through drug-herb interaction thereby affecting the therapeutic effect. This formulation can be considered as an adjunct to metformin in the management of diabetes mellitus.

Extraction and Ameliorative Effect of Camellia sinensis against toxicity induced by nickel nanoparticles in Sprague Dawley Rats

2020 ◽  
Vol 11 (10) ◽  
pp. 751-759
Author(s):  
Samreen Gul khan ◽  
Muhammad Farman ◽  
Umar Hayat ◽  
Ali Usman ◽  
Muhammad irfan ◽  
...  
Keyword(s):  
Camellia Sinensis ◽  
Nickel Nanoparticles ◽  
Phytochemical Analysis ◽  
Pharmacological Effects ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
The World ◽  
Ameliorative Effect ◽  
Appetite Stimulation

Camellia sinensis is extensively cultivated in most regions of the world. Camellia sinensis is known to have several pharmacological effects such as hypoglycemia, hypocholestrolemia, antioxidant, laxation, fungicide and appetite stimulation. Extraction of Camellia sinensis was done in Analytical lab of GC University Faisalabad. After extraction the extract were subjected to phytochemical analysis.

Target Organ Specificity: Diethylnitrosamine-and Dibenzylnitrosamine-induced Single-strand Breaks Plus Alkali-labile Bonds

1984 ◽  
Vol 3 (2) ◽  
pp. 207-216
Author(s):  
Douglas E. Brash ◽  
Cheng M. Su ◽  
Hani A. Nabi ◽  
Kathleen S. Reuter ◽  
Judith Ortman ◽  
...  
Keyword(s):  
Dna Damage ◽  
Chronic Administration ◽  
Target Organ ◽  
Single Strand ◽  
Organ Specificity ◽  
Tumor Promoters ◽  
Sprague Dawley ◽  
Strand Breaks ◽  
Single Strand Breaks ◽  
Sprague Dawley Rats

Quantitative dose response measurements of diethylnitrosamine-and dibenzylnitrosamine-induced DNA single-strand breaks plus alkali-labile bonds (SSB + ALB) (single-strand breaks, apurinic/apyrimidinic sites, and phosphotriesters) were performed on samples of 100,000 nuclei from Sprague-Dawley rats. Twenty-four hours after diethylnitrosamine injection, there were no SSB + ALB in brain (nontarget), SSB + ALB in kidney (target), and SSB + ALB in liver (target only after partial hepatectomy, tumor promoters, or chronic administration). Seven days after diethylnitrosamine injection, liver SSB + ALB had declined. The noncarcinogenic diethylnitrosamine analog, dibenzylnitrosamine, induced no SSB + ALB at 6 hours or 7 days postinjection. Induction of DNA damage, therefore, correlates well with target organ specificity of tumorigenesis when specificity is broadened to include those organs that exhibit tumors only when DNA damage is followed by additional treatments.

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