Extraction and Ameliorative Effect of Camellia sinensis against toxicity induced by nickel nanoparticles in Sprague Dawley Rats

2020 ◽  
Vol 11 (10) ◽  
pp. 751-759
Author(s):  
Samreen Gul khan ◽  
Muhammad Farman ◽  
Umar Hayat ◽  
Ali Usman ◽  
Muhammad irfan ◽  
...  
Keyword(s):  
Camellia Sinensis ◽  
Nickel Nanoparticles ◽  
Phytochemical Analysis ◽  
Pharmacological Effects ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
The World ◽  
Ameliorative Effect ◽  
Appetite Stimulation

Camellia sinensis is extensively cultivated in most regions of the world. Camellia sinensis is known to have several pharmacological effects such as hypoglycemia, hypocholestrolemia, antioxidant, laxation, fungicide and appetite stimulation. Extraction of Camellia sinensis was done in Analytical lab of GC University Faisalabad. After extraction the extract were subjected to phytochemical analysis.

scholarly journals Phytochemical analysis and hepatoprotective activity of Raphanus sativus var. sativus in Sprague-Dawley rats

2020 ◽  
Vol 19 (8) ◽  
pp. 1745-1752
Author(s):  
Heshu Sulaiman Rahman ◽  
Kashan Alaalddin Bayz ◽  
Ridha Hassan Hussein ◽  
Azad Ismael Abdalla ◽  
Hemn Hassan Othman ◽  
...  
Keyword(s):  
Liver Enzymes ◽  
Antioxidant Activities ◽  
Ethanol Extract ◽  
Hepatoprotective Activity ◽  
Phytochemical Analysis ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Hepatotoxic Effect ◽  
Hepatic Histopathology ◽  
Phenolic And Flavonoid

Purpose: To determine the phenolic and flavonoid contents of R. sativus rhizome ethanol extract and the hepatoprotective effect of the extract in rats. Methods: Folin–Ciocalteau and aluminum chloride colorimetric tests were used to determine the contents of phenols and flavonoids in the R. sativus extract. Male Sprague-Dawley rats induced with CCl4 to develop hepatotoxicity were treated orally with R. sativus extract for 4 weeks. The  antioxidant and anti-inflammatory effects of the extract on the liver were determined by evaluating the concentration of oxidative analytes, serum liver enzymes and lipids, and hepatic histopathology and cytochrome P450 2E1 expression. Results: R. sativus extract significantly (p < 0.05) reduced the hepatotoxic effect of CCl4 via its antioxidant activities and protection of liver tissues from oxidative damage. Conclusion: The hepatoprotective effects of R. sativus rhizome ethanol extract are attributed to its highphenolic and flavonoid contents. Keywords: R. savitus rhizome, Phenols, Flavonoid contents, antioxidant, Hepatoprotective

scholarly journals Comparison of the Pharmacological Effects of Paricalcitol and Doxercalciferol on the Factors Involved in Mineral Homeostasis

2010 ◽  
Vol 2010 ◽  
pp. 1-8 ◽  
Author(s):  
J. Ruth Wu-Wong ◽  
Masaki Nakane ◽  
Gerard D. Gagne ◽  
Kristin A. Brooks ◽  
William T. Noonan
Keyword(s):  
Vitamin D ◽  
Cell Proliferation ◽  
Mrna Expression ◽  
Active Form ◽  
Parathyroid Cell ◽  
Pharmacological Effects ◽  
Sprague Dawley ◽  
Mineral Homeostasis ◽  
Sprague Dawley Rats ◽  
Serum Pth

Vitamin D receptor agonists (VDRAs) directly suppress parathyroid hormone (PTH) mRNA expression. Different VDRAs are known to have differential effects on serum calcium (Ca), which may also affect serum PTH levels since serum Ca regulates PTH secretion mediated by the Ca-sensing receptor (CaSR). In this study, we compared the effects of paricalcitol and doxercalciferol on regulating serum Ca and PTH, and also the expression of PTH, VDR, and CaSR mRNA. The 5/6 nephrectomized (NX) Sprague-Dawley rats on a normal or hyperphosphatemia-inducing diet were treated with vehicle, paricalcitol, or doxercalciferol for two weeks. Both drugs at the tested doses (0.042–0.33 g/kg) suppressed PTH mRNA expression and serum PTH effectively in the 5/6 NX rats, but paricalcitol was less potent in raising serum Ca than doxercalciferol. In pig parathyroid cells, paricalcitol and the active form of doxercalciferol induced VDR translocation from the cytoplasm into the nucleus, suppressed PTH mRNA expression and inhibited cell proliferation in a similar manner, although paricalcitol induced the expression of CaSR mRNA more effectively. The multiple effects of VDRAs on modulating serum Ca, parathyroid cell proliferation, and the expression of CaSR and PTH mRNA reflect the complex involvement of the vitamin D axis in regulating the mineral homeostasis system.

scholarly journals TRANSFERSOMAL GEL CONTAINING GREEN TEA (CAMELLIA SINENSIS L. KUNTZE) LEAVES EXTRACT: INCREASING IN VITRO PENETRATION

2017 ◽  
Vol 10 (8) ◽  
pp. 294
Author(s):  
Effionora Anwar ◽  
Tahmida Diazputri Utami ◽  
Delly Ramadon
Keyword(s):  
Physicochemical Properties ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Spherical Shape ◽  
Entrapment Efficiency ◽  
Sprague Dawley ◽  
Tea Leaves ◽  
Sprague Dawley Rats ◽  
Green Tea Leaves

Objective: The aim of this study was to increase penetration of EGCG from green tea leaves extract (Camellia sinensis L. Kuntz) through the skin by formulating them into a transfersomal gel.Methods: Transfersomes were prepared by thin-layer hydration method, with different concentration of the extract that equivalent to 1% (F1), 1.5% (F2), and 2% (F3) EGCG. Transfersomes formula with good characteristics would be incorporated into a gel formulation. A gel without transfersomes prepared as a control of comparison. Both of gels were evaluated their physicochemical properties. In vitro penetration test using Franz diffusion cell with the skin of female Sprague-Dawley rats was also performed.Results: The results showed that F1 had the best physicochemical properties. F1 had a spherical shape, Dmean volume at 107.82±0.44 nm, polidispersity index at 0.07±0.01, zeta potential at -40.3±0.10 mV, and entrapment efficiency at 63.16±0.65%. Cumulative amount of EGCG penetrated from transfersomal and non-transfersomal gel were were 1302.63±20.67 μg/cm2 and 414.86±4.40 μg/cm2, resepctively (P<0.05). Flux penetration of transfersomal and non-transfersomal gel were was 57.594±0.91 μg/cm2.h and 36.144±1.22 μg/cm2.h, respectively.Conclusion: It can be concluded that transfersomal gel could increase the in vitro penetration of EGCG from green tea leaves extract compared to non-transfersomal one. 

Evaluation of reproductive and developmental toxicities of Pu-erh black tea (Camellia sinensis var. assamica) extract in Sprague Dawley rats

2013 ◽  
Vol 148 (1) ◽  
pp. 190-198 ◽  
Author(s):  
Di Wang ◽  
Jie Meng ◽  
Hui Gao ◽  
Kunlong Xu ◽  
Rong Xiao ◽  
...  
Keyword(s):  
Camellia Sinensis ◽  
Black Tea ◽  
Sprague Dawley ◽  
Sprague Dawley Rats

scholarly journals The Effect ofCamellia sinensison Wound Healing Potential in an Animal Model

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Fatemeh Hajiaghaalipour ◽  
M. S. Kanthimathi ◽  
Mahmood Ameen Abdulla ◽  
Junedah Sanusi
Keyword(s):  
Wound Healing ◽  
Camellia Sinensis ◽  
Inflammatory Cells ◽  
Positive Control ◽  
Sprague Dawley ◽  
Wound Area ◽  
Sprague Dawley Rats ◽  
Scar Width ◽  
Uniform Area ◽  
Wound Healing Activity

Camellia sinensis(tea) is reported to have health benefits, including the building of healthy skin. This study evaluated the effects of topical application ofCamellia sinensisextract on the rate of wound closure and the histology of wound area. A uniform area of 2.00 cm in diameter was excised from the neck of adult male Sprague Dawley rats. The animals were topically treated with 0.2 mL of vehicle (CMC), Intrasite gel (positive control), or 200 and 400 mg/mL of extract. Wounds dressed with the extract and Intrasite gel healed significantly earlier than those with vehicle. Histological analysis of the wound area after 10 days showed that wounds dressed with the extract had less scar width when compared to the control. The tissue contained less inflammatory cells and more collagen and angiogenesis, compared to wounds dressed with vehicle. In this study,Camellia sinensisshowed high potential in wound healing activity.

scholarly journals Ameliorative Effect of Quercetin on Methotrexate Induced Toxicity in Sprague-Dawley Rats: A Histopathological Study

2016 ◽  
Vol 50 (3s) ◽  
pp. S200-S208 ◽  
Author(s):  
Alexander Victor Anand David ◽  
Namani Satyanarayana ◽  
Subramani Parasuraman ◽  
Sengodan Bharathi ◽  
Radhakrishnan Arulmoli
Keyword(s):  
Histopathological Study ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Ameliorative Effect

scholarly journals Green Tea (Camellia sinensis) Extract Reduces Peptic Ulcer Induced by Helicobacter pylori in Sprague Dawley Rats

2020 ◽  
Vol 49 (11) ◽  
pp. 2793-2800
Author(s):  
Marhanis Salihah Omar ◽  
Nur Nadiana Adnan ◽  
Endang Kumolosasi ◽  
Norazrina Azmi ◽  
Noor Safwah Damanhuri ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Peptic Ulcer ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Sprague Dawley ◽  
Sprague Dawley Rats

scholarly journals Camellia sinensis extract phytosomes inhibit body weight gain in Sprague-Dawley rats

Pharmaciana ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 219
Author(s):  
Dwi Kurnia Putri ◽  
Iskandarsyah Iskandarsyah ◽  
Effionora Anwar
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Camellia Sinensis ◽  
Body Weight Gain ◽  
Sprague Dawley ◽  
Sprague Dawley Rats

scholarly journals Scientific Validation of Ethnomedicinal Use ofIpomoea batatasL. Lam. as Aphrodisiac and Gonadoprotective Agent against Bisphenol A Induced Testicular Toxicity in Male Sprague Dawley Rats

2019 ◽  
Vol 2019 ◽  
pp. 1-21 ◽  
Author(s):  
Muhammad Majid ◽  
Fatima Ijaz ◽  
Muhammad Waleed Baig ◽  
Bakht Nasir ◽  
Muhammad Rashid Khan ◽  
...  
Keyword(s):  
High Performance ◽  
Ipomoea Batatas ◽  
Semen Quality ◽  
Gonadal Hormones ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Sprague Dawley ◽  
Biochemical Tests ◽  
Sprague Dawley Rats

Sweet potato (Ipomoea batatasL. Lam.), known as “Shakarqandi” in Pakistan, is an imperative root vegetable with large size, traditionally used as aphrodisiac, antiprostatic, anti-inflammatory, antidiabetic, cardiotonic, and anticancer agent. Present study was conducted to gauge aphrodisiac potential ofIpomoea batatasethyl acetate (IPT-EA, IPA-EA) and methanol (IPT-M, IPA-M) extracts from tuber and aerial part, respectively, via behavioral and biochemical tests and their possible protective role in BPA-induced gonadotoxicity at the dose 300 mg/kg in male Sprague Dawley rats. Phytochemical analysis was done qualitatively and quantitatively through total phenolic and flavonoid content (TPC and TFC) and high performance liquid chromatographic (HPLC-DAD) fingerprinting while antioxidant profiling used multimodein vitroassays. To calculate sexual excitement mount latency, intromission latency, mount frequency, intromission frequency, ejaculatory latency, and postejaculatory interval were examined while for biochemical ratification semen characteristics, levels of testosterone, follicle stimulating hormone (FSH), luteinizing hormone (LH), and estradiol were measured. Gonadoprotective ability was assessed through comet assay and histomorphological examination of testes. Qualitative analysis ensured the presence of phenols, flavonoids, tannins, anthocyanin, saponins, coumarins, terpenoids, and betacyanin. Quantitatively maximal TPC (304.32±7.20 μg GAE/mg dry extract) and TFC (214.77±4.09 μg QE/mg DE) were estimated in IPA-EA extract. IPT-EA yielded maximum rutin (7.3±0.12) and myricetin (2.7±0.14 μg/mg DE) while IPA-EA and IPA-M yielded maximum caffeic acid (4.05±0.22 and 1.92±0.17 μg/mg DE, respectively) in HPLC-DAD analysis. Extracts enhanced sexual excitement, improved semen quality, levels of testosterone, FSH, LH, and estradiol, and successfully attenuated toxic effects of BPA. Levels of endogenous antioxidant enzymes (CAT, SOD, POD, and GSH) were restored and NO abundance was minimized. Significant stimulation in sexual behavior, amelioration of toxicity symptoms, elevated spermatic production, raised viability, vitalized levels of gonadal hormones, maintained endogenous enzymes, genoprotection, and reformed testicular histology endorsedI. batatasas a better aphrodisiac alternative and gonadoprotective agent.

scholarly journals STUDIES ON ACUTE TOXICITY OF HEPTOPLUS IN EXPERIMENTAL RATS

2016 ◽  
Vol 8 (11) ◽  
pp. 124
Author(s):  
M. Sankar ◽  
Johanna Rajkumar
Keyword(s):  
Acute Toxicity ◽  
Clinical Signs ◽  
Total Phenol ◽  
Vehicle Group ◽  
Phytochemical Analysis ◽  
Sprague Dawley ◽  
Oral Toxicity ◽  
Group I ◽  
Sprague Dawley Rats ◽  
Ld50 Value

Objective: To evaluate acute toxicity of Heptoplus (polyherbal drug) in Sprague-Dawley rats and to identify the active ingredients of the herbal drug.Methods: Heptoplus was subjected to preliminary phytochemical screening and the HPTLC fingerprint profile of herbal drug was documented. OECD guideline 423-Acute toxic class method was followed to evaluate the oral toxicity of Heptoplus in Sprague-Dawley rats. In acute toxicity studies, Group I (control) rats received 0.5% of carboxymethyl cellouse (Vehicle). Group II rats received 2000 mg/kg b. w of Heptoplus. The rats were observed on the day of dosing and thereafter for 14 d, for any toxic effect. Results: Preliminary phytochemical analysis of Heptoplus revealed total phenol, flavonoid, carbohydrate, and tannins as its major constituents. The total phenol and flavonoid content of Heptoplus was found to be 170 μg of gallic acid and 162 μg of quercetin equivalent. HPTLC analysis proved that phyllanthin is an active compound of Heptoplus. Acute oral toxicity assays showed Heptoplus administration did not result in any treatment-related mortality, abnormal clinical signs, and loss of body weight or gross pathological changes in rats. Hence, LD50 value of Heptoplus was found to be greater than 2000 mg/kg b. wt.Conclusion: Heptoplus contain phyllanthin as an active ingredient. LD50 value of Heptoplus was found to be greater than 2000 mg/kg b. wt.

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