scholarly journals Retracted: Antibacterial activity and mechanism of Tetrapleura tetraptera stem extract against Salmonella strains and its application in raw chicken meat

2021 ◽  
Keyword(s):  
Antibacterial Activity ◽  
Chicken Meat ◽  
Tetrapleura Tetraptera ◽  
Stem Extract

scholarly journals Green Synthesis, Characterization and Antibacterial Activity of Copper Oxide Nanoparticles Using Annona muricata Stem Extract

2021 ◽  
Vol 9 (12) ◽  
pp. 1205-1214
Author(s):  
Shivani Kushwaha
Keyword(s):  
Antibacterial Activity ◽  
Copper Oxide ◽  
Copper Sulphate ◽  
Copper Oxide Nanoparticles ◽  
Maximum Activity ◽  
Cuo Nanoparticles ◽  
Oxide Nanoparticles ◽  
Gradual Change ◽  
Annona Muricata ◽  
Stem Extract

Abstract: Nanotechnology is a rising field of science and technology that deals with the particles having size in the range of 1 to 100 nm. Copper oxide nanoparticles has many properties like antifungal activity, antibacterial activity, optical properties, conductive properties, etc. Due to its demand of diversified use, copper oxide nanoparticles were fabricated using ecofriendly and non-toxic Annona muricata stem extract. The extract with copper sulphate pentahydrate showed gradual change in the colour of the extract from brown to green which indicates the CuO nanoparticles synthesis. The fabrication is followed by characterization of CuO nanoparticles using UV-vis spectroscope, FTIR, XRD and SEM. The characterization showed roughly spherical shaped nanoparticles in the range of 100nm with high crystalline monoclinic phase. FTIR absorption spectra conclude that the compounds attached with copper oxide nanoparticles could be polyphenols with aromatic ring. The CuO nanoparticles exhibited antibacterial activity; it showed the maximum activity against E.coli (18 mm). Keywords: Annona muricata, copper sulphate pentahydrate, FTIR, nanomaterials, SEM, XRD.

scholarly journals Antibacterial and Cytotoxicity Activities of Major Compounds from Tinospora cordifolia Willd. Growing on Mangifera indica L.

2019 ◽  
Vol 3 (4) ◽  
pp. 32-42
Author(s):  
Thongchai Taechowisan
Keyword(s):  
Antibacterial Activity ◽  
Cell Lines ◽  
Crude Extract ◽  
Hek293 Cell ◽  
Colorimetric Assay ◽  
Mangifera Indica ◽  
Tinospora Cordifolia ◽  
Cytotoxicity Activity ◽  
Stem Extract ◽  
Cytotoxicity Activities

Objective To investigate the major constituents of Tinosporacordifolia Willd. growing on Mangiferaindica, and to evaluate the efficacy of their antibacterial and cytotoxicity activities. Methods The ethanolic stem extract of T. cordifolia was subjected to silica gel 60 column chromatography, thin layer chromatography and medium pressure liquid chromatography for isolation of the major compounds. Identification of purified compounds was achieved by spectroscopic methods.. The crude extract and purified compounds were screened for their antibacterial and cytotoxicity properties using standard procedures. Results Two alkaloids were purified and identified as Magnoflorin (1) and Tembetarine (2). These compounds showed high antibacterial activity against Bacillus cereus and Staphylococcus aureus with both MIC (32-64 µg/ml) and MBC (128-256 µg/ml). The cytotoxicity activity of the purified compounds and crude extract was determined using MTT colorimetric assay against L929 and HEK293 cell lines. This showed weak cytotoxicity activity with IC50 values of 1162.24 to 2290.00 µg/ml and 1376.67 to 2585.06 µg/ml towards L929 and HEK293 cell lines, respectively. Conclusion The major compounds present in ethanolic stem extract of T. cordifolia growing on M. indica were extracted, purified and identified. This study suggests that these compounds exhibit great potential for antibacterial activity with weak cytotoxicity activity. They may be useful for their medicinal functions.

scholarly journals FORMULASI GEL EKSTRAK BATANG PEPAYA SEBAGAI ANTIBAKTERI TERHADAP BAKTERI STAPHYLOCOCCUS AUREUS

2020 ◽  
Vol 4 (2) ◽  
pp. 1
Author(s):  
Amalia Eka Putri ◽  
Kristina Handayani
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Infectious Diseases ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Stem Extract ◽  
Gel Preparation ◽  
To Come ◽  
Important Disease

Infectious disease is an important disease in Indonesia. Infectious diseases can be caused by pathogenic microorganisms such as the bacterium Staphylococcus aureus ATCC 25923. Infectious diseases can be treated with antibiotics, but antibiotics now resistance.Therefore, other alternatives such as are needed papaya stem which can be used as an antibacterial against S.aureus ATCC 25923. The purpose of this study was to determine the antibacterial activity of papaya stem extract gel against Staphylococcus aureus ATCC 25923. Papaya stem sample extracted using a method soxhletation with 96% ethanol. The extract was made into a gel dosage with a concentration of 5%. The positive control used is clindamycin and the negative control is gel without extract. The results of the papaya stem gel antibacterial activity test showed that the papaya stem gel had antibacterial activity against S.aureus ATCC 25923. The papaya stem extract gel preparation with a concentration of 5% had an average inhibition zone diameter of 18,56±0,51mm. The antibacterial activity is thought to come from the flavonoids, tannins, and saponins contained in the papaya stem fraction.

scholarly journals Antibacterial Properties of Essential Oil (EO) Extracted from Nigella sativa Linn. (Black cumin) and its Application Against Vibrio cholerae in Ground Chicken Meat

2019 ◽  
Vol 34 (2) ◽  
pp. 55-60
Author(s):  
Sharmina Deloer ◽  
ML Bari ◽  
Md Mahfuzul Hoque
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Vibrio Cholerae ◽  
Chicken Meat ◽  
Optimum Temperature ◽  
Black Cumin ◽  
E Coli ◽  
Chemical Preservatives

Chemical preservatives for food preservation at high concentration are hazardous for public health. The natural antimicrobials from plants, herbs and spices have been given priority as alternatives of the chemical preservatives. In this study, antibacterial activity of the essential oil (3%) from black cumin seeds was tested against five food borne pathogens viz Staphylococcus aureus (ATCC 25923), Shigella dysenteriae-I (MJ-84), Escherichia coli 0157:H7 (ATCC 12079), Escherichia coli (ATCC 25922) and Vibrio cholerae( ATCC 6395) using disc diffusion method. The essential oil (EO) showed antibacterial activity against both Gram positive and Gram negative bacteria with the highest inhibition against S. aureus (17.4 ± 0.2) and the lowest against Vibrio cholerae (14.5 ± 1.0). No activity was observed against E. coli and E. coli O157:H7. The effect of temperature and pH on the antibacterial activity of the EO from black cumin seeds was determined. At 100°C for 30 min treatment, no significant reduction of antibacterial activity of the EO was observed, suggesting that high temperature does not affect the activity. The optimum temperature for the activity was recorded at 50°C. The EO showed the highest activity at pH 7.0 (optimum) with an insignificant change of activity at pH 5.0, 9.0 and 10.0. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of EO against S. aureus, V. cholerae and Shigella dysenterae were determined by two fold dilution method at optimum temperature of 50°C and 37oC and optimum pH at 7.0 and 5.0, respectively, which ranged from 0.125 (S. aureus) to 1.0% (Shigella dysenterae), with the MBC of 0.25 to 2.0%, respectively. Three times of MIC value of EO (1.5%) was applied in ground chicken meat seeded with V. cholerae ATCC 6395 and was kept at -20°C for 10 days. The test organism was diminished within 24 hours, suggesting that the EO from black cumin is highly active in vivo and could be used as preservative in ground chicken meat. The results of this study provide new dimension of research on EOs from spices against foodborne pathogens in vivo in controlling their growth in meat based food and food products. Bangladesh J Microbiol, Volume 34 Number 2 December 2017, pp 55-60

scholarly journals Screening of Methanolic and Aqueous Extracts of Lasimorpha senegalensis for Antibacterial Activity

2019 ◽  
pp. 1-7
Author(s):  
Anumudu Obinna H. ◽  
Akaniro Ifunanya R. ◽  
Ofonegbu Mercy N. ◽  
Ibediala John Kennedy C.
Keyword(s):  
Antibacterial Activity ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Biologically Active ◽  
Diffusion Method ◽  
Aqueous Extracts ◽  
Human Pathogens ◽  
Tetrazolium Chloride ◽  
Test Organisms ◽  
Stem Extract

Traditional medicine, though an old practice in disease prophylaxis and therapy, is still widely employed globally to treat various human ailments. In this study conducted at the Department of Microbiology, University of Nigeria between October 2018 and January 2019, methanolic and aqueous extracts of an aquatic plant Lasimorpha senegalensis were evaluated for antibacterial activities against human pathogens; Escherichia coli and Staphylococcus aureus. Agar well diffusion method was used to determine the potency of L. senegalensis against the test organisms at different concentrations. Also, minimum inhibitory concentration (MIC) was determined by tetrazolium chloride microtiter dilution assay. Results showed that; inhibition zone diameters ranging from 0-14 mm for both test organisms using the plant extracts was less than that of the control (septrin and chloramphenicol) ranging from 0-26 mm. MIC ranged from 62.5 mg/ml to 500 mg/ml, lowest MIC was obtained with methanolic stem extract. Preliminary phytochemical screening revealed the presence of flavonoids responsible for the antibacterial activity. Therefore, L. senegalensis should be considered medicinally important as they contain biologically active compounds with curative potentials against infectious diseases.

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