Hydrogen sulphide production by Saccharomyces cerevisiae UCD 522 in a synthetic grape juice medium deficient of thiamin (vitamin B 1 ) and/or pyridoxine (vitamin B 6 )

2019 ◽  
Vol 69 (5) ◽  
pp. 379-384
Author(s):  
H. Xing ◽  
C.G. Edwards
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Hydrogen Sulphide ◽  
Grape Juice ◽  
Vitamin B ◽  
Sulphide Production

Effects of Saccharomyces Cerevisiae Strains on Chemical Profiles of Cabernet Sauvignon Wines: Based on the Combined Results of 1H NMR, HS-SPME/GC-MS and HPLC-DAD-ESI-MS/MS

2018 ◽  
Vol 18 (2) ◽  
pp. 115-131
Author(s):  
Liang Heng-Yu ◽  
Su Ning ◽  
Guo Kun ◽  
Wang Yuan ◽  
Yang De-Yu
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Principal Component ◽  
Grape Juice ◽  
Pilot Scale ◽  
Ethyl Esters ◽  
Cabernet Sauvignon ◽  
Esi Ms ◽  
H Nmr ◽  
Chemical Profiles ◽  
Indigenous Yeasts

Five Saccharomyces cerevisiae strains (Chinese indigenous yeasts SC5, WC5, SC8, CC17 and commercial starter F15) were inoculated into Cabernet sauvignon grape must and fermented at pilot scale. For the first time, combination of 1H NMR, HS-SPME/GC-MS and HPLC-DAD-ESI-MS/MS metabonomic profiling techniques was performed to analyze the global chemical fingerprints of sampled wines at the end of alcoholic and malolactic fermentation respectively, then 13 non-volatile flavor compounds, 52 volatile organic aromas and 43 polyphenolic molecules were identified and determined correspondently. All principal component analysis (PCA) of two fermentation stages based on the analytical results of 1H NMR, HS-SPME/GC-MS and HPLC-DAD-ESI-MS/MS divided these strains into three clusters: (1) SC5 and SC8, (2) WC5 and F15 and (3) CC17. The wine fermented by indigenous yeast, CC17, showed a very unique chemical profile, such as low pH and high color intensity, reduced amino acids (including proline) and the lowest total higher alcohols levels, most of the fixed acids, glycerol, ethyl esters and anthocyanins concentrations. The statistical results indicate that CC17 strain possesses very special anabolism and catabolism abilities on such substances in grape juice and has potentiality to produce characteristic wines with high qualities.

scholarly journals Starmerella bombicola and Saccharomyces cerevisiae in Wine Sequential Fermentation in Aeration Condition: Evaluation of Ethanol Reduction and Analytical Profile

Foods ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1047
Author(s):  
Laura Canonico ◽  
Edoardo Galli ◽  
Alice Agarbati ◽  
Francesca Comitini ◽  
Maurizio Ciani
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Global Climate ◽  
Grape Juice ◽  
Preliminary Screening ◽  
Aeration Condition ◽  
Volatile Acidity ◽  
Starmerella Bombicola ◽  
Ethanol Content ◽  
Condition Evaluation ◽  
Sequential Fermentation

In the last few decades, the increase of ethanol in wine, due to global climate change and consumers’ choice is one of the main concerns in winemaking. One of the most promising approaches in reducing the ethanol content in wine is the use of non-Saccharomyces yeasts in co-fermentation or sequential fermentation with Saccharomyces cerevisiae. In this work, we evaluate the use of Starmerella bombicola and S. cerevisiae in sequential fermentation under aeration condition with the aim of reducing the ethanol content with valuable analytical profile. After a preliminary screening in synthetic grape juice, bench-top fermentation trials were conducted in natural grape juice by evaluating the aeration condition (20 mL/L/min during the first 72 h) on ethanol reduction and on the analytical profile of wines. The results showed that S. bombicola/S. cerevisiae sequential fermentation under aeration condition determined an ethanol reduction of 1.46% (v/v) compared with S. cerevisiae pure fermentation. Aeration condition did not negatively affect the analytical profile of sequential fermentation S. bombicola/S. cerevisiae particularly an overproduction of volatile acidity and ethyl acetate. On the other hand, these conditions strongly improved the production of glycerol and succinic acid that positively affect the structure and body of wine.

scholarly journals Impact of Pharmacological Inhibition of Hydrogen Sulphide Production in the SOD1G93A-ALS Mouse Model

2019 ◽  
Vol 20 (10) ◽  
pp. 2550 ◽  
Author(s):  
Alida Spalloni ◽  
Viviana Greco ◽  
Giulia Ciriminna ◽  
Victor Corasolla Carregari ◽  
Federica Marini ◽  
...  
Keyword(s):  
Hydrogen Sulphide ◽  
Histological Evaluation ◽  
Dual Inhibitor ◽  
Number Of Factors ◽  
Sporadic Als ◽  
Sulphide Production ◽  
High Concentrations ◽  
Als Patients ◽  
H2s Production ◽  
Lateral Sclerosis

A number of factors can trigger amyotrophic lateral sclerosis (ALS), although its precise pathogenesis is still uncertain. In a previous study done by us, poisonous liquoral levels of hydrogen sulphide (H2S) in sporadic ALS patients were reported. In the same study very high concentrations of H2S in the cerebral tissues of the familial ALS (fALS) model of the SOD1G93A mouse, were measured. The objective of this study was to test whether decreasing the levels of H2S in the fALS mouse could be beneficial. Amino-oxyacetic acid (AOA)—a systemic dual inhibitor of cystathionine-β-synthase and cystathionine-γ lyase (two key enzymes in the production of H2S)—was administered to fALS mice. AOA treatment decreased the content of H2S in the cerebral tissues, and the lifespan of female mice increased by approximately ten days, while disease progression in male mice was not affected. The histological evaluation of the spinal cord of the females revealed a significant increase in GFAP positivity and a significant decrease in IBA1 positivity. In conclusion, the results of the study indicate that, in the animal model, the inhibition of H2S production is more effective in females. The findings reinforce the need to adequately consider sex as a relevant factor in ALS.

The THI5 gene family of Saccharomyces cerevisiae: distribution of homologues among the hemiascomycetes and functional redundancy in the aerobic biosynthesis of thiamin from pyridoxine

Microbiology ◽  
2003 ◽  
Vol 149 (6) ◽  
pp. 1447-1460 ◽  
Author(s):  
Raymond Wightman ◽  
Peter A. Meacock
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Gene Family ◽  
Biosynthetic Pathway ◽  
Growth Conditions ◽  
Anaerobic Growth ◽  
Gene Copy ◽  
Phylogenetic Study ◽  
Vitamin B ◽  
Mrna Levels ◽  
Quadruple Mutant

The THI5 gene family of Saccharomyces cerevisiae comprises four highly conserved members named THI5 (YFL058w), THI11 (YJR156c), THI12 (YNL332w) and THI13 (YDL244w). Each gene copy is located within the subtelomeric region of a different chromosome and all are homologues of the Schizosaccharomyces pombe nmt1 gene which is thought to function in the biosynthesis of hydroxymethylpyrimidine (HMP), a precursor of vitamin B1, thiamin. A comprehensive phylogenetic study has shown that the existence of THI5 as a gene family is exclusive to those yeasts of the Saccharomyces sensu stricto subgroup. To determine the function and redundancy of each of the S. cerevisiae homologues, all combinations of the single, double, triple and quadruple deletion mutants were constructed using a PCR-mediated gene-disruption strategy. Phenotypic analyses of these mutant strains have shown the four genes to be functionally redundant in terms of HMP formation for thiamin biosynthesis; each promotes synthesis of HMP from the pyridoxine (vitamin B6) biosynthetic pathway. Furthermore, growth studies with the quadruple mutant strain support a previous proposal of an alternative HMP biosynthetic pathway that operates in yeast under anaerobic growth conditions. Comparative analysis of mRNA levels has revealed subtle differences in the regulation of the four genes, suggesting that they respond differently to nutrient limitation.

Prediction of hydrogen sulphide production during anaerobic digestion of organic substrates

2012 ◽  
Vol 121 ◽  
pp. 419-424 ◽  
Author(s):  
Pascal Peu ◽  
Sylvie Picard ◽  
Arnaud Diara ◽  
Romain Girault ◽  
Fabrice Béline ◽  
...  
Keyword(s):  
Anaerobic Digestion ◽  
Hydrogen Sulphide ◽  
Organic Substrates ◽  
Sulphide Production

scholarly journals Amiloride Uptake and Toxicity in Fission Yeast Are Caused by the Pyridoxine Transporter Encoded by bsu1+ (car1+)

2005 ◽  
Vol 4 (2) ◽  
pp. 319-326 ◽  
Author(s):  
Jürgen Stolz ◽  
Heike J. P. Wöhrmann ◽  
Christian Vogl
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Plasma Membrane ◽  
Multidrug Resistance ◽  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Vitamin B ◽  
Acidic Ph ◽  
Ph Optimum ◽  
Sodium Transporters ◽  
High Affinity

ABSTRACT Amiloride, a diuretic drug that acts by inhibition of various sodium transporters, is toxic to the fission yeast Schizosaccharomyces pombe. Previous work has established that amiloride sensitivity is caused by expression of car1 +, which encodes a protein with similarity to plasma membrane drug/proton antiporters from the multidrug resistance family. Here we isolated car1 + by complementation of Saccharomyces cerevisiae mutants that are deficient in pyridoxine biosynthesis and uptake. Our data show that Car1p represents a new high-affinity, plasma membrane-localized import carrier for pyridoxine, pyridoxal, and pyridoxamine. We therefore propose the gene name bsu1 + (for vitamin B6 uptake) to replace car1 +. Bsu1p displays an acidic pH optimum and is inhibited by various protonophores, demonstrating that the protein works as a proton symporter. The expression of bsu1 + is associated with amiloride sensitivity and pyridoxine uptake in both S. cerevisiae and S. pombe cells. Moreover, amiloride acts as a competitor of pyridoxine uptake, demonstrating that both compounds are substrates of Bsu1p. Taken together, our data show that S. pombe and S. cerevisiae possess unrelated plasma membrane pyridoxine transporters. The S. pombe protein may be structurally related to the unknown human pyridoxine transporter, which is also inhibited by amiloride.

scholarly journals Hydrogen Sulphide Production by Non-multiplying Organisms and its Inhibition by Antibiotics

1954 ◽  
Vol 11 (2) ◽  
pp. 160-174 ◽  
Author(s):  
A. L. Olitzki
Keyword(s):  
Hydrogen Sulphide ◽  
Sulphide Production

Oxidative stress induces smooth muscle dedifferentiation in placental stem villus arteries through reduced hydrogen sulphide production by cystathionine-γ-lyase

Placenta ◽  
2013 ◽  
Vol 34 (9) ◽  
pp. A27
Author(s):  
Liangjian Lu ◽  
Graham J. Burton ◽  
Tereza Cindrova-Davies
Keyword(s):  
Oxidative Stress ◽  
Smooth Muscle ◽  
Hydrogen Sulphide ◽  
Sulphide Production

scholarly journals The SSU1 checkup, a rapid tool for detecting chromosomal rearrangements of the Saccharomyces cerevisiae chromosome XVI. An ecological and technological study on wine yeast

2020 ◽  
Author(s):  
Philippe Marullo ◽  
Olivier Claisse ◽  
Maria Laura Raymond Eder ◽  
Marine Börlin ◽  
Nadine Feghali ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Chromosomal Rearrangements ◽  
Decisive Role ◽  
Grape Juice ◽  
Wine Yeast ◽  
Single Experiment ◽  
Pcr Method ◽  
Rapid Characterization ◽  
Technological Study ◽  
And Inversion

1)AbstractChromosomal rearrangements (CR) such as translocations, duplications and inversions play a decisive role in the adaptation of microorganisms to specific environments. In enological Saccharomyces cerevisiae strains, CR involving the promoter region of the gene SSU1 lead to a higher sulfite tolerance by enhancing the SO2 efflux. To date, three different SSU1 associated CR events have been described, including translocations XV-t-XVI and VIII-t-XVI and inversion inv-XVI. In the present study, we developed a multiplex PCR method (SSU1 check-up) that allows a rapid characterization of these three chromosomal configurations in a single experiment. Nearly 600 S. cerevisiae strains collected from fermented grape juice were genotyped by microsatellite markers. We demonstrated that alleles of the SSU1 promoter are differently distributed according to the wine environment (cellar versus vineyard) and the nature of the grape juice. Moreover, rearranged SSU1 promoters are significantly enriched among commercial starters. In addition, nearly isogenic strains collected in similar environments show different CR suggesting that translocation events occur with a non-negligible frequency in clonal populations likely due to mitotic recombination events. Finally, the link between the nature of SSU1 promoter and the tolerance to sulfite was statistically validated in natural grape juice containing various SO2 concentrations. The SSU1 check-up is therefore a convenient new tool for addressing population genetics questions and for selecting yeast strains by using molecular markers.

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