Low Vitamin D and High Cholesterol facilitate oral carcinogenesis in 4NQO induced rat models via regulating glycolysis

Deficiency of Vitamin D is linked to an increased risk of hypertension, peripheral artery disease, and myocardial infarction and is a major risk factor for the development of human atherosclerosis. Atheromatous cytokines, including TNF-α, IL-6 and IFN-γ, and EGF receptor family growth factors are released at the site of atherosclerosis and act on proteolytic enzymes, MMPs (matrix metalloproteinases), ADAMs (a disintegrin and metalloproteinases), and ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs). ADAM-12 activates EGFR resulting in increased migration and proliferation of smooth muscle cells (SMCs). The aim of this study was to examine the effect of vitamin D on IL-6-induced ADAM-12 expression and SMC migration and proliferation. Micro-swine were fed with either vitamin D-deficient high cholesterol diet or high cholesterol diet containing 900 IU of vitamin D for 6 months. After six months when serum cholesterol levels ranged from 500-600 mg/dL, vitamin D-deficient group continued on the same deficient diet, whereas the other group received supplementation of vitamin D (1,000 IU/d) for 6 months. The mRNA expression of ADAM-12 and EGFR in whole carotid artery and in IL-6-treated SMCs was quantified by qPCR. The proliferation was assayed by CyQuant NF cell proliferation assay. The mRNA transcripts of ADAM-12 and EGFR were significantly increased in carotid arteries from Vitamin D-deficient than in vitamin D- supplemented swine. Treatment of SMCs with IL-6 also increased the mRNA transcripts of ADAM-12 and EGFR in vitamin D-deficient swine SMCs compared to vitamin D-supplemented swine SMCs. The cell proliferation was higher in SMCs isolated from Vitamin D-deficient swine carotid artery compared to vitamin D- supplemented swine carotid artery. Together, these results suggest that Vitamin D regulates ADAM-12-mediated activation of EGFR and vitamin D deficiency further enhances proliferation of SMCs, which is potentiated by atheromatous cytokines.

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The Use of Dietary Supplements Among African and Caribbean Women Living in the UK: A Cross-Sectional Study

Nutrients ◽

10.3390/nu12030847 ◽

2020 ◽

Vol 12 (3) ◽

pp. 847

Author(s):

Amanda Rodrigues Amorim Adegboye ◽

Omorogieva Ojo ◽

Gulshanara Begum

Keyword(s):

Vitamin D ◽

Dietary Supplements ◽

Dietary Supplement ◽

High Cholesterol ◽

Black African ◽

Supplement Use ◽

Caribbean Women ◽

Black Caribbean ◽

Dietary Supplement Use ◽

The Uk

Previous studies have shown that the use of dietary supplements is associated with the prevention of birth defects, negative pregnancy outcomes and cardiovascular diseases. However, there might be some ethnic disparities in supplement usage suggesting that women who could benefit from it are not frequent users. This study aimed to characterise the use of dietary supplement among Black African and Black Caribbean women living in the United Kingdom (UK). Furthermore, it evaluated possible associations between the use of dietary supplements and health and diet awareness. A total of 262 women self-ascribed as Black African and Black Caribbean living in the UK completed a comprehensive questionnaire on socio-demographic factors, diet, use of supplements and cultural factors. The main outcome variable was the regular use of any type of dietary supplement. Use of vitamin D and/or calcium was also explored. A stepwise logistic regression analysis was applied to identify predictors of regular use of dietary supplements. A total of 33.2% of women reported regular use of any dietary supplements and 16.8% reported use of vitamin D and/or calcium. There were no significant ethnic differences in the use of dietary supplements. Reporting use of the back of food packaging label (odds ratio (OR) 2.21; 95% CI 1.07–4.55); a self-rated healthy diet (OR 2.86; 95% CI 1.19–6.91) and having cardiovascular disease (CVD), hypertension and/or high cholesterol (OR 3.81; 95% CI 1.53–9.49) increased the likelihood of using any dietary supplement. However, having poorer awareness decreased the likelihood (OR 0.94; 95% CI 0.88–0.99) of using any dietary supplement. For the use of vitamin D and/or calcium supplements, the main predictor was having CVD, hypertension and/or high cholesterol (OR 4.43; 95% CI 1.90–10.35). The prevalence of dietary supplement use was low among African and Caribbean women. Thus, awareness of potential benefits of some dietary supplements (e.g., vitamin D) among the Black population should be promoted.

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Cholesterol in pregnancy: a review of knowns and unknowns

Obstetric Medicine ◽

10.1258/om.2011.110003 ◽

2011 ◽

Vol 4 (4) ◽

pp. 147-151 ◽

Cited By ~ 27

Author(s):

Änne Bartels ◽

Keelin O'Donoghue

Keyword(s):

Cardiovascular Disease ◽

Vitamin D ◽

Fetal Development ◽

Plasma Cholesterol ◽

Adult Population ◽

The Body ◽

High Cholesterol ◽

Cholesterol Levels ◽

Growing Body ◽

In Pregnancy

Cholesterol forms part of every cell in the human body, and also helps make and metabolize hormones, bile acids and vitamin D. Human plasma cholesterol levels are determined by production in the liver and by dietary intake. Lipoproteins carry cholesterol around the body, and facilitate it crossing the placenta. Cholesterol is carefully monitored in the non-pregnant adult population, where its association with atherosclerosis and cardiovascular disease is well understood. Although it is known that cholesterol rises in pregnancy, at present it is not routinely measured or treated. The effects of maternal high cholesterol on pregnancy and on fetal development are not yet fully understood. However, a growing body of evidence from animal and human studies suggests adverse consequences of high cholesterol levels in pregnancy.

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The nutritional, hormonal and vitamin status in overweight and obesity

International Journal of Research in Medical Sciences ◽

10.18203/2320-6012.ijrms20170173 ◽

2017 ◽

Vol 5 (2) ◽

pp. 678

Author(s):

Snaa A. Kamel ◽

Mohammed K. Alelwan ◽

Ahmed S. Alqahtani

Keyword(s):

Vitamin D ◽

Vitamin B12 ◽

Weight Control ◽

Overweight And Obesity ◽

Nutritional Deficiencies ◽

High Cholesterol ◽

Obese People ◽

Global Epidemic ◽

Vitamin Status ◽

Males And Females

Background: Obesity is a global epidemic which is increasing dramatically in overwhelming rates among males and females of all age categories in developing and developed countries.Methods: The first phase of the project involves the questionnaires which apply to all the Participants in research. We also use the lab investigation to determine the abnormality in overweight and obese people. Then we will analysis and combine the results to others research and give output and recommendation that we hope helping us to limiting of increase incidence of obesity.Results: sample show that high cholesterol above normal average approximately in 31.05% of the participants while the triglyceride rate increased in 31.67%. A lack of vitamin D, as the rate did not exceed 18±6.8, in individuals with the BMI 25-29, and to be 13±8.82, in individuals with the BMI>30. Vitamin B12 rate became borderline in individuals with BMI 25-29, and deficient in individuals with BMI>30.Conclusions: Obese Saudi individuals have several nutritional deficiencies specifically in vitamins D and B12 despite their overweight. Efforts should be focused to increase awareness of healthy and weight control.

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High Fat Diet and High Cholesterol Diet Reduce Hepatic Vitamin D‐25‐Hydroxylase Expression and Serum 25‐Hydroxyvitamin D 3 Level through Elevating Circulating Cholesterol, Glucose, and Insulin Levels

Molecular Nutrition & Food Research ◽

10.1002/mnfr.202100220 ◽

2021 ◽

pp. 2100220

Author(s):

Tengfei Zhu ◽

Jingyu Zhao ◽

Shu Zhuo ◽

Zhimin Hu ◽

Shuyu Ouyang ◽

...

Keyword(s):

Vitamin D ◽

High Fat Diet ◽

High Cholesterol Diet ◽

Cholesterol Diet ◽

High Cholesterol ◽

High Fat ◽

Insulin Levels ◽

Hydroxyvitamin D ◽

25 Hydroxyvitamin D

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Abstract 469: Vitamin D Deficiency Potentiates Restenosis Following Coronary Angioplasty in Hypercholesterolemic Swine

Arteriosclerosis Thrombosis and Vascular Biology ◽

10.1161/atvb.32.suppl_1.a469 ◽

2012 ◽

Vol 32 (suppl_1) ◽

Author(s):

Gaurav K Gupta ◽

Tanupriya Agrawal ◽

Michael G Del Core ◽

William J Hunter ◽

Devendra K Agrawal

Keyword(s):

Vitamin D ◽

Coronary Artery ◽

Vitamin D Deficiency ◽

Balloon Angioplasty ◽

Coronary Intervention ◽

Vitamin D Supplementation ◽

High Cholesterol Diet ◽

Cholesterol Diet ◽

High Cholesterol ◽

Increased Risk

Introduction: Vitamin D is a sectosteroid that functions through vitamin D receptor (VDR), a transcription factor, which regulates various downstream signaling pathways and controls the transcription of many targets genes. Vitamin D deficiency is associated increased risk of incident congestive heart failure, myocardial infarction, and cardiovascular disease mortality. However, most of the studies which identified an association between lower vitamin D intake or lower 25-hydroxyvitamin D and increased risk of cardiovascular disease are observational or cross-sectional studies. There has been no careful study evaluating the effect of vitamin D supplementation on coronary artery disease outcome following coronary intervention. Objective: The purpose of this study was to examine the effect of vitamin D deficiency and vitamin D supplementation on coronary artery restenosis following balloon angioplasty in hypercholesterolemic Yucatan microswine. Methods: Twelve female microswine were fed vitamin D-deficient or vitamin D-sufficient high cholesterol diet. At six months, animals underwent coronary angioplasty. Following coronary intervention swine in the vitamin D-sufficient high cholesterol diet group received supplementation of 1,000 IU or 3,000 IU of vitamin D3. Six months later, angiogram was performed followed by optical coherence tomography to monitor the development of intimal hyperplasia and restenosis. At the end of 12 months animals were euthanized, coronary arteries were harvested and morphological and histological studies were performed. Results: Findings from the optical coherence tomography and histomorphometric studies showed a significant decrease in neointimal hyperplasia, increase in in-segment lumen diameter, and decrease in the intima-media ratio in coronary arteries following balloon angioplasty in swine with vitamin D supplementation compared to the animals with vitamin D-deficient status. In the in-vitro studies, calcitriol inhibited proliferation of coronary artery smooth muscle cells. Conclusion: These data suggest that vitamin D deficiency increases intimal hyperplasia and restenosis following coronary balloon angioplasty in hypercholesterolemic swine. Since vitamin D inhibits smooth muscle cell proliferation, supplementation of non-secosteroidal VDR ligands prior to coronary intervention could help in preventing the neointimal formation and restenosis.

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Abstract 500: Vitamin D Supplementation Attenuates ADAM-12-mediated Proliferation of Carotid Artery Smooth Muscle Cells of Hypercholesterolemic Swine

Arteriosclerosis Thrombosis and Vascular Biology ◽

10.1161/atvb.35.suppl_1.500 ◽

2015 ◽

Vol 35 (suppl_1) ◽

Author(s):

Vikash Kansal ◽

Eric B Patterson

Keyword(s):

Vitamin D ◽

Smooth Muscle ◽

Carotid Artery ◽

Protein Expression ◽

Vitamin D Supplementation ◽

High Cholesterol Diet ◽

Cholesterol Diet ◽

High Cholesterol ◽

Tnf Α ◽

Adam 12

Introduction: Risk of hypertension, peripheral artery disease, myocardial infarction and development of human atherosclerosis has been linked to vitamin D deficiency. Atheromatous cytokines, including IL-6, TNF-α and epidermal growth factor receptor (EGFR) family growth factors are released at the site of atherosclerosis and boost the activity of proteolytic enzymes such as ADAMs (a disintegrin and metalloproteinases). ADAM-12 cleaves proHB-EGF (Heparin-binding EGF-like growth factor) activating EGFR, resulting in increased proliferation of smooth muscle cells (SMCs). The aim of this study was to examine the effect of vitamin D on IL-6 and TNF-α-induced ADAM-12, pEGFR expression, HB-EGF release and SMC proliferation. Methods: Micro-swine were fed with vitamin D-deficient high cholesterol diet, high cholesterol diet containing 900 IU of vitamin D, and high cholesterol diet containing 3000 IU of vitamin D for total of 12 months. After six months, serum cholesterol levels of 500-600 mg/dL were achieved in all the three groups. The protein expression of ADAM-12 & pEGFR, and HB-EGF release, in presence or absence of IL-6, TNF-α and Calcitriol, in SMCs was quantified by western Blot. HB-EGF release was measured by ELISA. The proliferation was assayed by [3H]-Thymidine incorporation and cell counting method. Results: The protein expression of ADAM-12 & pEGFR, HB-EGF release were significantly reduced in carotid artery SMCs isolated from Vitamin D-supplemented swine. IL-6 and TNF-α treatment increased the protein expression of ADAM-12 & pEGFR and HB-EGF release in carotid artery SMCs. Proliferation capacity was higher in SMCs isolated from Vitamin D-deficient swine carotid artery, potentiated by IL-6 and TNF-α. Calcitriol inhibited the ADAM-12, pEGFR expression and HB-EGF released in SMCs of hypercholesterolemic swine. Calcitriol also inhibited the proliferation of carotid artery SMCs isolated from Vit D-deficient, D-sufficient and D-supplemented swine. Conclusion: Together, these results suggest that vitamin D deficiency enhances proliferation of SMCs, which is potentiated by atheromatous cytokines. Whereas, vitamin D supplementation regulates ADAM-12-mediated cleavage of proHB-EGF and activation of EGFR inhibiting SMC proliferation.

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Effects of low-dose ketanserin on atherosclerosis in rats and rabbits

Canadian Journal of Physiology and Pharmacology ◽

10.1139/y10-079 ◽

2010 ◽

Vol 88 (11) ◽

pp. 1054-1060 ◽

Cited By ~ 8

Author(s):

Yong-Sheng Yu ◽

He-Hui Xie ◽

Ling Li ◽

Shu-Wei Song ◽

Ping Han ◽

...

Keyword(s):

Blood Pressure ◽

Vitamin D ◽

New Zealand ◽

Treatment Group ◽

Low Dose ◽

Control Group ◽

High Cholesterol Diet ◽

Cholesterol Diet ◽

High Cholesterol ◽

New Zealand White Rabbits

The present study was designed to test the hypothesis that a small dose of ketanserin, which enhances baroreflex activity, prevents the early lesions of atherosclerosis. In experiment 1, baroreflex sensitivity (BRS) was measured in 31 spontaneously hypertensive rats (SHRs) in a conscious state using a computerized blood pressure monitoring system. Four weeks later, the rats were administered vitamin D3 and fed a high-cholesterol diet for 8 weeks to induce atherosclerosis. Then their hearts and aortae were removed for pathological examination. A negative correlation was found between BRS and the scores of coronary (r = –0.460, P < 0.01) and aortic atherosclerosis (r = –0.448, P < 0.05) in SHR. In experiment 2, SHRs were divided into 3 groups (n = 10 in each group) and received a dose of ketanserin of 0.3, 1.0, and 3.0 mg/kg (i.g.), respectively. At the smallest dose (0.3 mg/kg), ketanserin did not lower blood pressure but enhanced BRS. In experiment 3, SHRs were administered vitamin D3, fed a high-cholesterol diet, and simultaneously treated with low-dose ketanserin. The atherosclerosis scores of the treatment group were significantly lower than those of the control group (coronary score: 0.90 ± 0.14 vs. 1.76 ± 0.27, P < 0.05; aortic scores: 1.00 ± 0.39 vs. 2.18 ± 0.41, P < 0.05). In experiment 4, male New Zealand White rabbits were fed a high-cholesterol diet and treated with low-dose ketanserin at the same time. The atherosclerosis scores of the treatment group were significantly lower than those of the control group (aortic scores: 0.26 ± 0.20 vs. 0.60 ± 0.31, P < 0.05). In conclusion, the present study demonstrated, for the first time, that low-dose ketanserin prevented the development of atherosclerosis independent of its blood pressure lowering action in SHRs and New Zealand White rabbits at least in part via enhancement of arterial baroreflex function.

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