scholarly journals Development of an efficient marker‐free soybean transformation method using the novel bacterium Ochrobactrum haywardense H1

2022 ◽  
Author(s):  
Hyeon‐Je Cho ◽  
York Moy ◽  
Nathan A. Rudnick ◽  
Theodore M. Klein ◽  
Jiaming Yin ◽  
...  
Keyword(s):  
Transformation Method ◽  
The Novel ◽  
Soybean Transformation ◽  
Novel Bacterium ◽  
Marker Free

scholarly journals Citroniella saccharovorans gen. nov. sp. nov., a member of the family Peptoniphilaceae isolated from a human fecal sample from a coastal traditional community member

2019 ◽  
Vol 69 (4) ◽  
pp. 1142-1148 ◽  
Author(s):  
Nisha B. Patel ◽  
Alexandra J. Obregón-Tito ◽  
Raul Y. Tito ◽  
Omar Trujillo-Villaroel ◽  
Luis Marin-Reyes ◽  
...  
Keyword(s):  
Fecal Sample ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
The Novel ◽  
Content Type ◽  
Link Type ◽  
Novel Bacterium ◽  
The Family ◽  
Diamino Acid ◽  
Rrna Gene Sequencing

A novel Gram-stain-positive, non-motile, non-spore-forming coccus-shaped obligately anaerobic bacterium was recovered from a fecal sample obtained from an individual from a traditional community located on the southern coast of Peru. The results of analysis based on 16S rRNA gene sequencing indicated the novel bacterium to be phylogenetically distinct from other genera of members of the Peptoniphilaceae family, sharing a loose affinity with the genera Ezakiella , Finegoldia , Gallicola and Parvimonas . The major cellular fatty acids of the novel isolate were determined to be C16:0, C17:1ω8c, and C18:1ω9c. The DNA G+C content was 29.9 mol%. End products of metabolism from peptone yeast glucose broth (PYG) were determined to be acetate and methyl succinate. The diagnostic diamino acid present in the cell wall was lysine. On the basis of the phenotypic, chemotaxonomic and phylogenetic results the organism is a member of a novel genus belonging to the family Peptoniphilaceae for which the name Citroniella saccharovorans gen nov. sp. nov., is proposed. The type strain is M6.X9T (DSM 29873T=CCUG 66799T).

Roseibium limicola sp. nov., isolated from tidal mudflat

2021 ◽  
Vol 71 (11) ◽  
Author(s):  
Veeraya Weerawongwiwat ◽  
Jong-Hwa Kim ◽  
Jung-Hoon Yoon ◽  
Min Kuk Suh ◽  
Han Sol Kim ◽  
...  
Keyword(s):  
Type Species ◽  
Rrna Gene ◽  
The Novel ◽  
Respiratory Quinone ◽  
Protein Coding ◽  
Content Type ◽  
Link Type ◽  
Novel Bacterium ◽  
Ubiquinone 10 ◽  
Single Flagellum

A novel bacterium, designated strain CAU 1637T, was isolated from a tidal mudflat. Cells of strain CAU 1637T were Gram-stain-negative, aerobic, motile with single flagellum and rod-shaped. The optimum conditions for growth were observed at 30 °C, pH 6.0 and in the presence of 2 % (w/v) NaCl. The respiratory quinone was ubiquinone-10. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CAU 1637T was closely related to the genus Roseibium , with the highest similarity to Roseibium aestuarii NRBC 112946T (97.4 %), followed by Roseibium hamelinense NRBC 16783T (96.8 %), Roseibium aquae JCM 19310T (96.4 %), Roseibium sediminis KCTC 52373T (95.8 %) and Roseibium denhamense JCM 10543T (95.3 %). The predominant cellular fatty acids were C18 : 1  ω7c 11-methyl and summed feature 8 (C18 : 1  ω7c and/or C18 : 1  ω6c). The major polar lipids consisted of diphosphatidylglycerol and phosphatidylglycerol. The average nucleotide identity values between the novel isolate and related strains ranged from 71.0 to 76.4 %, and the DNA−DNA hybridization values ranged from 19.3 to 20.3 %. The G+C content was 58.4 mol% and the whole-genome size was 4.6 Mb, which included 17 contigs and 3931 protein-coding genes. Based on the taxonomic data, strain CAU 1637T represents a novel species of the genus Roseibium , for which the name Roseibium limicola sp. nov. is proposed. The type strain is CAU 1637T (=KCTC 82429T=MCCC 1K06080T).

Optimization of Bending Process of Large Marine Crankthrow by Computer Simulation

2007 ◽  
Vol 561-565 ◽  
pp. 1949-1952 ◽  
Author(s):  
Ming Yue Sun ◽  
Shan Ping Lu ◽  
Dian Zhong Li ◽  
Yi Yi Li
Keyword(s):  
Computer Simulation ◽  
Conventional Method ◽  
Transformation Method ◽  
Actual Process ◽  
The Novel ◽  
Fem Model ◽  
Maximum Resistance ◽  
Bending Process ◽  
Novel Method ◽  
Simulation Results

The conventional method of bending the large crankthrow was investigated by computer simulation combined with manufacturing trial, and the typical forging defects, such as constricted waist, folded cracks and horn mouth on forged blank were analyzed. On the basis of these results, a novel forging shape of preformed blank was proposed using anti-transformation method by computer simulation. The FEM simulated results show that all the above defects can be avoided by carrying out the novel method, furthermore, the maximum resistance of the novel bending deformation was reduced to 72% of conventional process, and the weight of forging blank can be decreased by 15%. Finally, the optimum forging shape was applied to the actual process, the simulation results were confirmed by manufacturing trial, and qualified forged piece was gained. The FEM model established can be used for further optimization of other types of crankthrow.

Unusual mortality incidents in tit species (family Paridae) associated with the novel bacterium Suttonella ornithocola

2006 ◽  
Vol 158 (6) ◽  
pp. 203-205 ◽  
Author(s):  
J. K. Kirkwood ◽  
S. K. Macgregor ◽  
H. Malnick ◽  
G. Foster
Keyword(s):  
The Novel ◽  
Novel Bacterium

scholarly journals Sediminitomix flava gen. nov., sp. nov., of the phylum Bacteroidetes, isolated from marine sediment

2007 ◽  
Vol 57 (8) ◽  
pp. 1689-1693 ◽  
Author(s):  
Shams Tabrez Khan ◽  
Yasuyoshi Nakagawa ◽  
Shigeaki Harayama
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Marine Sediment ◽  
Physiological Data ◽  
Rrna Gene ◽  
The Novel ◽  
Okinawa Island ◽  
Novel Bacterium ◽  
Novel Genus And Species ◽  
Marine Sediment Sample

A novel bacterium designated Mok-1-85T was isolated from a marine sediment sample collected from Okinawa Island, Japan. Cells of strain Mok-1-85T stained Gram-negative, were catalase- and oxidase-positive and were non-motile. In a neighbour-joining tree based on 16S rRNA gene sequences, the novel strain clustered with the genus Flammeovirga, a member of the family ‘Flammeovirgaceae’. The novel isolate shared low 16S rRNA gene sequence similarities (≤86 %) with the members of the genus Flammeovirga and other related taxa. The major isoprenoid quinone was MK-7 and the predominant fatty acids of this organism were iso-C15 : 0, C16 : 1 ω5c and C16 : 0 3-OH. The G+C content of the DNA was 38 mol%. Combined phylogenetic and physiological data showed that strain Mok-1-85T represents a novel genus and species for which the name Sediminitomix flava gen. nov., sp. nov. is proposed. The type strain is Mok-1-85T (=NBRC 101625T=KCTC 12970T=CIP 109411T).

scholarly journals Helicobacter mesocricetorum sp. nov., a Novel Helicobacter Isolated from the Feces of Syrian Hamsters

2000 ◽  
Vol 38 (5) ◽  
pp. 1811-1817 ◽  
Author(s):  
J. H. Simmons ◽  
L. K. Riley ◽  
C. L. Besch-Williford ◽  
C. L. Franklin
Keyword(s):  
Alkaline Phosphatase ◽  
Sequence Analysis ◽  
Type Strain ◽  
Rrna Gene ◽  
The Novel ◽  
Accession Number ◽  
Syrian Hamsters ◽  
Novel Bacterium ◽  
Microaerobic Conditions ◽  
The 16S Rrna Gene

A spiral-shaped bacterium with bipolar, single, nonsheathed flagella was isolated from the feces of Syrian hamsters. The bacterium grew as a thin spreading film at 37°C under microaerobic conditions, did not hydrolyze urea, was positive for catalase and alkaline phosphatase, reduced nitrate to nitrite, did not hydrolyze hippurate, and was sensitive to nalidixic acid but resistant to cephalothin. Sequence analysis of the 16S rRNA gene and biochemical and phenotypic criteria indicate that the novel bacterium is a helicobacter. The novel bacterium is most closely related to the recently described mouse enteric helicobacter, Helicobacter rodentium. This is the first urease-negative Helicobacter species with nonsheathed flagella isolated from feces of asymptomatic Syrian hamsters. We propose to name this novel helicobacter Helicobacter mesocricetorum. The type strain is MU 97-1514 (GenBank accession number AF072471).

scholarly journals Sphingomonas hengshuiensis sp. nov., isolated from lake wetland

2015 ◽  
Vol 65 (Pt_12) ◽  
pp. 4644-4649 ◽  
Author(s):  
Shuzhen Wei ◽  
Tingting Wang ◽  
Hongliang Liu ◽  
Caifeng Zhang ◽  
Jiping Guo ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Optimal Growth ◽  
Northern China ◽  
Rrna Gene ◽  
The Novel ◽  
Respiratory Quinone ◽  
Closely Related Species ◽  
Novel Bacterium ◽  
The Status ◽  
Type Strains

A polyphasic taxonomic study was undertaken to establish the status of a novel bacterium, designated strain WHSC-8T, which was isolated from soil of Hengshui Lake Wetland Reserve in Hebei province, northern China. Colonies of this strain were yellow and cells were rod-shaped, polar-flagellated and obligately aerobic, exhibiting negative Gram reaction. The strain was able to grow at 0–1 % (w/v) NaCl, pH 5–10 and 20–35 °C, with optimal growth occurring at pH 7.0 and 28 °C without NaCl. Chemotaxonomic data revealed that strain WHSC-8T possesses ubiquinone Q-10 as the predominant respiratory quinone, C18 : 1ω7c, C16 : 0 and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH) as the major fatty acids, and sym-homospermidine as the major polyamine. Sphingomonadaceae-specific sphingoglycolipid was detected in the polar lipid patterns. The G+C content of the genomic DNA was 68.7 mol%. All of the above characters corroborated the assignment of the novel strain to the genus Sphingomonas. Strain WHSC-8T shared less than 97.0 % 16S rRNA gene sequence similarity with the type strains of other species of the genus Sphingomonas, except for Sphingomonas asaccharolytica DSM 10564T (97.5 %). The low DNA–DNA relatedness value and distinct phenotypic and chemotaxonomic characteristics distinguished strain WHSC-8T from closely related species of the genus Sphingomonas. Therefore, strain WHSC-8T represents a novel species of the genus Sphingomonas, for which the name Sphingomonas hengshuiensis sp. nov. is proposed. The type strain is WHSC-8T ( = KCTC 42455T = CCTCC AB 2015265T).

scholarly journals A novel and effective Cre/lox-based genetic tool for repeated, targeted and markerless integration in Yarrowia lipolytica

2020 ◽  
Author(s):  
Qinghua Zhou ◽  
Liangcheng Jiao ◽  
Wenjuan Li ◽  
Zhiming Hu ◽  
Yunchong Li ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Protein Production ◽  
Genome Engineering ◽  
Selection Marker ◽  
The Novel ◽  
Genetic Tool ◽  
Recombination System ◽  
Marker Free ◽  
Free Gene ◽  
Gene Integration

AbstractThe unconventional yeast Yarrowia lipolytica is extensively applied in bioproduction fields owing to its excellent metabolite and protein production ability. Nonetheless, utilization of this promising host is still restricted by limited availability of precise and effective gene integration tools. In this study, a novel and efficient genetic tool was developed for targeted, repeated, and markerless gene integration based on Cre/lox sitespecific recombination system. The developed tool required only a single selection marker and could completely excise all of the unnecessary sequences. A total of three plasmids were created and seven rounds of marker-free gene integration were examined in Y. lipolytica. All the integration efficiencies remained above 90%, and analysis of protein production and growth characteristics of the engineered strains confirmed that genome modification via the novel genetic tool was feasible. Further work also confirmed the genetic tool was effective for integration of other genes, loci, and strains. Thus, this study significantly promotes the application of Cre/lox system and presents a powerful tool for genome engineering in Y. lipolytica.

scholarly journals Augmentation of dietary glucosylceramide hydrolysis by the novel bacterium Glucerabacter canisensis NATH-2371T

2018 ◽  
Vol 82 (12) ◽  
pp. 2191-2197
Author(s):  
Misho Kawata ◽  
Masato Suzuki ◽  
Shoko Akutsu ◽  
Natsuki Kawahara ◽  
Ami Tsukamoto ◽  
...  
Keyword(s):  
The Novel ◽  
Novel Bacterium
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