Evaluation of Capsaicinoids Extracts as Bioactive Substance for Antimicrobial Films

2013 ◽  
Vol 64 (2) ◽  
Author(s):  
Tan Wee Leng ◽  
Ida Idayu Muhamad ◽  
Dayang Norulfairuz Abang Zaidel ◽  
Nozieana Khairuddin
Keyword(s):  
Antimicrobial Properties ◽  
Food Preservation ◽  
Active Components ◽  
E Coli ◽  
Agar Diffusion Test ◽  
Antimicrobial Film ◽  
Capsicum Spp ◽  
Bioactive Substance ◽  
Antioxidant Agent ◽  
Chili Peppers

Capsaicinoid, a naturally occurring alkaloid group and active components which can be found in chili peppers variety (Capsicum spp.), contributes to the pungency, taste, and aroma of chili peppers. Apart from their culinary and medicinal properties, capsaicinoid functions as antioxidant agent in food preservation. In the current research, the evaluation of bio-active substance of capsaicinoid was carried out to determine its potential in development of antimicrobial film for packaging. A mixture of acetone: petroleum ether (1:1) had been used in extracting the capsaicinoids and carotenoids (natural red pigment).The four types of chili pepper studied were Green Malagueta Salvador, Red Malagueta Salvador, red Thai Capsicum Frutescens and red Cayenne. The optimum capsaicinoids extraction time and amount of carotenoids (color intensity) for each variety of chili peppers were studied. Lastly, antimicrobial properties of AM films were determined as they inhibited both gram-positive (Streptococcus and B. subtilis) and gram-negative (E. coli) bacteria successfully through agar diffusion test and liquid culture test.

scholarly journals Phytochemical and Antimicrobial Properties of the Aqueous Root Extract of Triclisia dictyophylla

2013 ◽  
Vol 4 (1) ◽  
pp. 15-20
Author(s):  
Anslem O Ajugwo ◽  
Anthony C Ezimah ◽  
Francis M Awah ◽  
Philippe E Mounbegna ◽  
Celestine C Azikiwe
Keyword(s):  
Staphylococcus Aureus ◽  
Antifungal Activity ◽  
Antimicrobial Properties ◽  
Phytochemical Analysis ◽  
Root Extract ◽  
Medical Sciences ◽  
Active Components ◽  
E Coli ◽  
Sensitivity Pattern ◽  
Aqueous Root Extract

Triclisia dictyophylla have been used traditionally for the treatment of different ailments. The root was therefore subjected to phytochemical analysis and antimicrobial/antifungal activity against some hospital-strain disease causing microorganisms. Standard methods were used for the phytochemical screening. The extract was subjected to antimicrobial/antifungal activity using Staphylococcus aureus, Escherichia coli, Klebsiella spp, Pseudomonas aeruginosa and Candida albicans. The sensitivity pattern was compared to that of ciprofloxacin, cotrimoxazole and ofloxacin. Phytochemical analysis revealed mostly alkaloids and protein while tannins, glycoside and saponin were revealed in trace (+) amounts. The extract showed a good antimicrobial activity on Staphylococcus aureus isolated from sputum and on E. coli from both urine and sputum with no antifungal activity. The extract contains active components which could be harnessed for formulation of antibiotics.DOI: http://dx.doi.org/10.3126/ajms.v4i1.7846   Asian Journal of Medical Sciences 4(2013) 15-20  

scholarly journals SYNTHESIS, CHARACTERIZATION, AND ASSESSMENT OF THE ANTIMICROBIAL ACTIVITY OF MGO – CALCIUM ALGINATE POROUS BEADS

2017 ◽  
Vol 26 (2) ◽  
pp. 7
Author(s):  
Oscar Perales Pérez ◽  
Yarilyn CedeñoMattei
Keyword(s):  
Antimicrobial Activity ◽  
Calcium Alginate ◽  
Antimicrobial Agents ◽  
Physical Stability ◽  
Antimicrobial Properties ◽  
Volume Ratio ◽  
Food Preservation ◽  
Polymeric Matrix ◽  
E Coli ◽  
Porous Beads

La dispersión de nanopartículas antibacterianas en matrices poliméricas biocompatibles, no tóxicas y biodegradables permitirá el desarrollo de materiales más eficientes y efectivos para la conservación de alimentos, la eliminación de contaminantes y la protección contra microorganismos que comprometen la salud humana. Los materiales bactericidas nanométricos tienen una relación superficie / volumen muy grande que les permite interactuar con más copias de moléculas biológicas, y por lo tanto, mejorar la eficacia antimicrobiana. Más recientemente, se ha sugerido la actividad antimicrobiana del MgO amigable con el medio ambiente y químicamente estable. La incorporación de compuestos bactericidas en una matriz polimérica puede combinar la estabilidad física proporcionada por la matriz polimérica con las propiedades antimicrobianas de los agentes antimicrobianos dispersados como partıculas pequeñas sólidas. Sobre esta base, la presente investigación se centrará en el desarrollo de mezclas de partículas inorgánicas poliméricas biocompatibles, los denominados nanocompuestos, con actividad antimicrobiana sintonizable y mejorada. Se confirmó la actividad antimicrobiana de perlas de alginato cálcico - MgO (que oscilaban entre 0% y 40% p / p MgO) contra E. coli. Las perlas que contenían 20% p / p de MgO inhibían completamente el crecimiento bacterial de la E. coli. Palabras clave.-Alginato de calcio, Cuentas porosas, Óxido de magnesio, Actividad antimicrobiana. ABSTRACTThe dispersion of antibacterial nanoparticles into bio-compatible, non-toxic and bio-degradable polymeric matrices will enable the development of more efficient and effective materials for food preservation, removal of contaminants, and protection against human health-compromising microorganisms. Nanometric bactericidal materials have a very large surface to volume ratio that enable them to attach more copies of biological molecules, and hence, enhance antimicrobial efficiency. More recently, the antimicrobial activity of environmental-friendly and chemically stable MgO has been suggested. The incorporation of bactericidal compounds into a polymeric matrix can combine physical stability provided by the polymeric matrix with the antimicrobial properties of antimicrobial agents dispersed as solid tiny particles. On this basis, the present research will be focused on the development of biocompatible polymer-inorganic particle mixtures, so-called nanocomposites, with tunable and enhanced antimicrobial activity. The antimicrobial activity of calcium alginate – MgO beads (ranging from 0% - 40% w/w MgO) against E. coli was confirmed. Beads containing 20% w/w of MgO fully inhibited the E. coli. bacterial growth.. Keywords.- Calcium alginate, Porous beads, Magnesium oxide, Antimicrobial activity.

Disinfection of Fruits with Activated Water

2019 ◽  
Vol 10 ◽  
pp. 1864-1872
Author(s):  
Prof. Teodora P. Popova
Keyword(s):  
Antimicrobial Activity ◽  
Aqueous Solutions ◽  
Antimicrobial Properties ◽  
The Other ◽  
Gram Negative Bacteria ◽  
High Antimicrobial Activity ◽  
Gram Negative ◽  
E Coli ◽  
Number Of Microorganisms ◽  
Lower Effect

The effect of ionized aqueous solutions (anolytes and catholyte) in the processing of fruits (cherries, morellos, and strawberries) for decontamination has been tested. Freshly prepared analytes and catholyte without the addition of salts were used, as well as stored for 7 months anolytes, prepared with 0.5% NaCl and a combination of 0.5% NaCl and 0.5% Na2CO3. The anolyte prepared with a combination of 0.5% NaCl and 0.5% Na2CO3, as well as the anolyte obtained with 0.5% NaCl, exhibit high antimicrobial activity against the surface microflora of strawberries, cherries, and sour cherries. They inactivate E. coli for 15 minutes. The other species of the fam. Enterobacteriaceae were also affected to the maximum extent, as is the total number of microorganisms, especially in cherries and sour cherries. Even stored for 7 months, they largely retain their antimicrobial properties. Anolyte and catholyte, obtained without the addition of salts, showed a lower effect on the total number of microorganisms, but had a significant effect on Gram-negative bacteria, and especially with regard to the sanitary indicative E. coli.

scholarly journals Physicochemical and Antimicrobial Properties of Cocoa Pod Husk Pectin Intended as a Versatile Pharmaceutical Excipient and Nutraceutical

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Ofosua Adi-Dako ◽  
Kwabena Ofori-Kwakye ◽  
Samuel Frimpong Manso ◽  
Mariam EL Boakye-Gyasi ◽  
Clement Sasu ◽  
...  
Keyword(s):  
Aqueous Media ◽  
Antimicrobial Properties ◽  
Microbiological Quality ◽  
Elemental Content ◽  
Moderate Activity ◽  
Pharmaceutical Excipient ◽  
E Coli ◽  
Swelling Capacity ◽  
Weak Activity ◽  
Cocoa Pod Husk

The physicochemical and antimicrobial properties of cocoa pod husk (CPH) pectin intended as a versatile pharmaceutical excipient and nutraceutical were studied. Properties investigated include pH, moisture content, ash values, swelling index, viscosity, degree of esterification (DE), flow properties, SEM, FTIR, NMR, and elemental content. Antimicrobial screening and determination of MICs against test microorganisms were undertaken using agar diffusion and broth dilution methods, respectively. CPH pectin had a DE of 26.8% and exhibited good physicochemical properties. Pectin had good microbiological quality and exhibited pseudoplastic, shear thinning behaviour, and high swelling capacity in aqueous media. The DE, FTIR, and NMR results were similar to those of previous studies and supported highly acetylated low methoxy pectin. CPH pectin was found to be a rich source of minerals and has potential as a nutraceutical. Pectin showed dose-dependent moderate activity against gram positive and gram negative microorganisms but weak activity against Listeria spp. and A. niger. The MICs of pectin ranged from 0.5 to 4.0 mg/mL, with the highest activity against E. coli and S. aureus (MIC: 0.5–1.0 mg/mL) and the lowest activity against A. niger (MIC: 2.0–4.0 mg/mL). The study has demonstrated that CPH pectin possesses the requisite properties for use as a nutraceutical and functional pharmaceutical excipient.

scholarly journals Design, Synthesis and Biological Evaluation of Novel Benzothiazole Based [1,2,4]Triazolo[4,3-c]quinazoline Derivatives

2020 ◽  
Vol 32 (3) ◽  
pp. 580-586
Author(s):  
Ranjit V. Gadhave ◽  
Bhanudas S. Kuchekar
Keyword(s):  
Biological Evaluation ◽  
Bacterial Strains ◽  
Active Compounds ◽  
Design Synthesis ◽  
Structural Modifications ◽  
E Coli ◽  
Chemical Structures ◽  
Ft Ir ◽  
Antioxidant Agent

A new series of N-(benzo[d]thiazol-2-yl)-[1,2,4]triazolo[4,3-c]quinazoline-5-carboxamide derivatives were synthesized by condensation of [1,2,4]triazolo[4,3-c]quinazoline-5-carboxylate derivatives with substituted benzothiazoles. The chemical structures of the synthesized compounds were confirmed by FT-IR, MS and 1H NMR spectra. Designed triazoloquinazoline derivatives were docked with oxido-reductase enzyme (PDB Code 4h1j) and DNA gyrase enzyme (PDB Code 3g75). Based on high binding affinity score, the best compound were selected for synthesis and subjected to in vitro antioxidant and antibacterial activity. Compounds 7a and 7d were found to be most active compounds as antioxidant agent among this series when compared with ascorbic acid. Compounds 7a, 7d and 7f were found to be most active compounds as an antibacterial agents among this series when compared with ciprofloxacin against bacterial strains such as S. aureus (ATCC 25923), E. coli (ATCC 25922) and P. aeruginosa (ATCC 27853). Study revealed that the most active compounds after structural modifications can be exploited as lead molecules for other pharmacological activities such as anti-inflammatory, anticancer and antidepressant activities.

Antimicrobial Screening, in Silico Studies and QSAR of Chalcone-based 1,4-disubstituted 1,2,3-triazole Hybrids

Drug Research ◽  
2020 ◽  
Author(s):  
Pinki Yadav ◽  
Kashmiri Lal ◽  
Ashwani Kumar
Keyword(s):  
Topoisomerase Ii ◽  
Antimicrobial Properties ◽  
Qsar Model ◽  
Quantitative Structure Activity Relationship ◽  
Binding Modes ◽  
E Coli ◽  
Structure Activity ◽  
In Silico Studies ◽  
Microbial Strains

AbstractThe in vitro antimicrobial properties of some chalcones (1a–1c ) and chalcone tethred 1,4-disubstituted 1,2,3-triazoles (2a–2u) towards different microbial strains viz. Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Aspergillus niger and Candida albicans are reported. Compounds 2g and 2u exhibited better potency than the standard Fluconazole with MIC values of 0.0063 µmol/mL and 0.0068 µmol/mL, respectively. Furthermore, molecular docking was performed to investigate the binding modes of two potent compounds 2q and 2g with E. coli topoisomerase II DNA gyrase B and C. albicans lanosterol 14α-demethylase, respectively. Based on these results, a statistically significant quantitative structure activity relationship (QSAR) model was successfully summarized for antibacterial activity against B. subtilis.

scholarly journals Conditions to Prolonged Release of Microencapsulated Carvacrol on Alginate Films as Affected by Emulsifier Type and PH

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
Silvia Matiacevich ◽  
Natalia Riquelme ◽  
María Lidia Herrera
Keyword(s):  
Storage Time ◽  
Antimicrobial Agents ◽  
Antimicrobial Properties ◽  
Edible Film ◽  
Initial Time ◽  
Tween 20 ◽  
Prolonged Release ◽  
E Coli ◽  
Fresh Foods

Alginate from algal biomass is used as edible film and the incorporation of antimicrobial agents improves its performance to increase the shelf-life of fresh foods. However, environmental conditions and intrinsic properties of films influence their release. The aim of this study was to investigate the effect of the concentration and type of encapsulating agent and pH of emulsions on the physical and antimicrobial properties of alginate-carvacrol films. Films containing alginate, carvacrol as antimicrobial agent, and Tween 20 or trehalose (0.25 and 0.75% w/w) as encapsulating agents were obtained from suspensions at pH 4 and pH 8. Physical characterization of emulsions and films and antimicrobial properties (E. coliandB. cinerea) was evaluated. Results showed that droplets size depended on trehalose concentration, but emulsion stability depended on pH and type of encapsulating agent, being more stable samples with trehalose at pH 4. Although films with Tween 20 presented the highest opacity, they showed the best antimicrobial properties at initial time; however, during storage time, they lost their activity before samples with trehalose and relative humidity (RH) was the principal factor to influence their release. Therefore, sample formulated with 0.25% trehalose at pH 4 and stored at 75% RH had the best potential as edible film for fresh fruits.

scholarly journals Synthesis and Antimicrobial Properties of Highly Cross-Linked pH-Sensitive Hydrogels through Gamma Radiation

Polymers ◽  
2021 ◽  
Vol 13 (14) ◽  
pp. 2223
Author(s):  
Moises Bustamante-Torres ◽  
Victor H. Pino-Ramos ◽  
David Romero-Fierro ◽  
Sandra P. Hidalgo-Bonilla ◽  
Héctor Magaña ◽  
...  
Keyword(s):  
Bacterial Infections ◽  
Antimicrobial Agents ◽  
Film Formation ◽  
Antimicrobial Properties ◽  
Ph Sensitive ◽  
Scanning Calorimetry ◽  
Polymeric Systems ◽  
E Coli ◽  
High Prevalence

The design of new polymeric systems for antimicrobial drug release focused on medical/surgical procedures is of great interest in the biomedical area due to the high prevalence of bacterial infections in patients with wounds or burns. For this reason, in this work, we present a new design of pH-sensitive hydrogels copolymerized by a graft polymerization method (gamma rays), intended for localized prophylactic release of ciprofloxacin and silver nanoparticles (AgNPs) for potential topical bacterial infections. The synthesized hydrogels were copolymerized from acrylic acid (AAc) and agar. Cross-linked hydrogel film formation depended on monomer concentrations and the degree of radiation used (Cobalt-60). The obtained hydrogel films were characterized by attenuated total reflectance Fourier-transform infrared spectroscopy (ATR-FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), and mechanical testing. The swelling of the hydrogels was evidenced by the influence of their pH-sensitiveness. The hydrogel was loaded with antimicrobial agents (AgNPs or ciprofloxacin), and their related activity was evaluated. Finally, the antimicrobial activity of biocidal-loaded hydrogel was tested against Escherichia coli (E. coli) and methicillin-resistant Staphylococcus aureus (MRSA) on in vitro conditions.

scholarly journals In vitro evaluation of the antimicrobial activity of five root canal sealers

2004 ◽  
Vol 15 (1) ◽  
pp. 30-35 ◽  
Author(s):  
Brenda Paula Figueiredo de Almeida Gomes ◽  
José Assis Pedroso ◽  
Rogério Castilho Jacinto ◽  
Morgana Eli Vianna ◽  
Caio Cezar Randi Ferraz ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Properties ◽  
Contact Method ◽  
Streptococcus Sanguis ◽  
Actinomyces Naeslundii ◽  
Agar Diffusion Test ◽  
Significant Difference ◽  
Ah Plus ◽  
Endodontic Sealers

The aim of the present study was to analyze the antimicrobial properties of five endodontic sealers: Endo Fill, Endomethasone, Endomethasone N, Sealer 26 and AH-Plus, against the following microorganisms: Candida albicans, Staphylococcus aureus, Enterococcus faecalis, Streptococcus sanguis and Actinomyces naeslundii. The sealers were tested immediately, 24 h, 48 h and 7 days after manipulation.The direct contact method through the observation of the microbial growth in liquid medium and the agar diffusion test were used to evaluate the antimicrobial properties of the sealers. The results, in both methodologies used, showed that immediately after manipulation, Endo-Fill and Endomethasone demonstrated the highest antimicrobial activity, with no statistically significant difference between them. Sealer 26 demonstrated the lowest antimicrobial activity. At all other times after manipulation, there were no statistically significant differences among all the sealers tested. In conclusion, none of the sealers totally inhibited the growth of the microorganisms. Furthermore, the antimicrobial activity of each sealer decreased with time and was dependent upon the microbial susceptibility to them.

scholarly journals Antimicrobial activities of laboratory produced essential oil solutions against five selected fungal strains

2013 ◽  
pp. 171-183 ◽  
Author(s):  
Emilija Ivanova ◽  
Natalija Atanasova-Pancevska ◽  
Dzoko Kungulovski
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Antimicrobial Properties ◽  
Microtiter Plate ◽  
Antimicrobial Activities ◽  
Food Preservation ◽  
Minimal Inhibitory Concentrations ◽  
Fungal Strains ◽  
Treat Ment ◽  
Penicillium Spp

It is well known that essential oils possess significant antimicrobial activity. This study was conducted to estimate the antimicrobial activity of various types of Biokill, a laboratory produced solution composed of several essential oils (Biokill dissolved in 96% ethanol; Biokill 96% further dissolved in DMSO; Biokill dissolved in 70% ethanol and Biokill 70% further dissolved in DMSO). The antimicrobial activity was evaluated against five selected fungal strains, Candida albicans ATCC 10231, Saccharomyces cerevisiae ATCC 9763, Aspergillus niger I.N. 1110, Aspergillus sojae CCF and Penicillium spp. FNS FCC 266. A variation of the microtiter plate-based antimicrobial assay was used in order to assess the antimicrobial activity of the solutions. By applying this assay minimal inhibitory concentrations (MIC) of the Biokill solutions were determined for each strain of the selected test microorganisms. The results demonstrated that all variations of Biokill showed antimicrobial activity at concentrations lower than 2.5?g/mL. Biokill 70% further dissolved in DMSO showed the best antimicrobial properties against all the selected strains with MICs less than 1.25?g/mL. These results indicated that Biokill could find application in the pharmaceutical industry, in food preservation and conservation, in the prevention and treat?ment of plants infected by certain phytopathogens, etc.

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