scholarly journals Constrained Tibial Vibration in Mice: A Method for Studying the Effects of Vibrational Loading of Bone

2008 ◽  
Vol 130 (4) ◽  
Author(s):  
Blaine A. Christiansen ◽  
Philip V. Bayly ◽  
Matthew J. Silva
Keyword(s):  
Soft Tissue ◽  
Frequency Response ◽  
Natural Frequency ◽  
Lower Leg ◽  
Bone Strain ◽  
Fe Model ◽  
Loading Method ◽  
Vibrational Loading ◽  
Controlled Conditions

Vibrational loading can stimulate the formation of new trabecular bone or maintain bone mass. Studies investigating vibrational loading have often used whole-body vibration (WBV) as their loading method. However, WBV has limitations in small animal studies because transmissibility of vibration is dependent on posture. In this study, we propose constrained tibial vibration (CTV) as an experimental method for vibrational loading of mice under controlled conditions. In CTV, the lower leg of an anesthetized mouse is subjected to vertical vibrational loading while supporting a mass. The setup approximates a one degree-of-freedom vibrational system. Accelerometers were used to measure transmissibility of vibration through the lower leg in CTV at frequencies from 20Hzto150Hz. First, the frequency response of transmissibility was quantified in vivo, and dissections were performed to remove one component of the mouse leg (the knee joint, foot, or soft tissue) to investigate the contribution of each component to the frequency response of the intact leg. Next, a finite element (FE) model of a mouse tibia-fibula was used to estimate the deformation of the bone during CTV. Finally, strain gages were used to determine the dependence of bone strain on loading frequency. The in vivo mouse leg in the CTV system had a resonant frequency of 60Hz for ±0.5G vibration (1.0G peak to peak). Removing the foot caused the natural frequency of the system to shift from 60Hzto70Hz, removing the soft tissue caused no change in natural frequency, and removing the knee changed the natural frequency from 60Hzto90Hz. By using the FE model, maximum tensile and compressive strains during CTV were estimated to be on the cranial-medial and caudolateral surfaces of the tibia, respectively, and the peak transmissibility and peak cortical strain occurred at the same frequency. Strain gage data confirmed the relationship between peak transmissibility and peak bone strain indicated by the FE model, and showed that the maximum cyclic tibial strain during CTV of the intact leg was 330±82με and occurred at 60–70Hz. This study presents a comprehensive mechanical analysis of CTV, a loading method for studying vibrational loading under controlled conditions. This model will be used in future in vivo studies and will potentially become an important tool for understanding the response of bone to vibrational loading.

scholarly journals Numerical model of optical coherence tomographic vibrography imaging to estimate corneal biomechanical properties

2014 ◽  
Vol 11 (101) ◽  
pp. 20140920 ◽  
Author(s):  
Sabine Kling ◽  
Imran B. Akca ◽  
Ernest W. Chang ◽  
Giuliano Scarcelli ◽  
Nandor Bekesi ◽  
...  
Keyword(s):  
Frequency Response ◽  
Response Function ◽  
Frequency Response Function ◽  
Corneal Thickness ◽  
Biomechanical Properties ◽  
Invasive Technique ◽  
Fe Model ◽  
Optical Coherence ◽  
Biomechanical Parameters

Most techniques measuring corneal biomechanics in vivo are biased by side factors. We demonstrate the ability of optical coherence tomographic (OCT) vibrography to determine corneal material parameters, while reducing current prevalent restrictions of other techniques (such as intraocular pressure (IOP) and thickness dependency). Modal analysis was performed in a finite-element (FE) model to study the oscillation response in isolated thin corneal flaps/eye globes and to analyse the dependency of the frequency response function on: corneal elasticity, viscoelasticity, geometry (thickness and curvature), IOP and density. The model was verified experimentally in flaps from three bovine corneas and in two enucleated porcine eyes using sound excitation (100–110 dB) together with a phase-sensitive OCT to measure the frequency response function (range 50–510 Hz). Simulations showed that corneal vibration in flaps is sensitive to both, geometrical and biomechanical parameters, whereas in whole globes it is primarily sensitive to corneal biomechanical parameters only. Calculations based on the natural frequency shift revealed that flaps of the posterior cornea were 0.8 times less stiff than flaps from the anterior cornea and cross-linked corneas were 1.6 times stiffer than virgin corneas. Sensitivity analysis showed that natural vibration frequencies of whole globes were nearly independent from corneal thickness and IOP within the physiological range. OCT vibrography is a promising non-invasive technique to measure corneal elasticity without biases from corneal thickness and IOP.

scholarly journals Digital image correlation and finite element modelling as a method to determine mechanical properties of human soft tissue in vivo

2016 ◽  
Author(s):  
Kevin Mattheus Moerman
Keyword(s):  
Mechanical Properties ◽  
Soft Tissue ◽  
Bulk Material ◽  
Constitutive Models ◽  
Image Correlation ◽  
Fe Model ◽  
Fe Modelling ◽  
Tissue Phantom ◽  
Human Soft Tissue

The mechanical properties of human soft tissue are crucial for impactbiomechanics, rehabilitation engineering and surgical simulation.Validation of these constitutive models using human data remainschallenging and often requires the use of non-invasive imaging and inversefinite element (FE) analysis. Post processing data from imaging methodssuch as tagged magnetic resonance imaging (MRI) can be challenging. DigitalImage Correlation (DIC) however is a relatively straightforward imagingmethod and thus the goal of this study was to assess the use of DIC incombination with FE modelling to determine the bulk material properties ofhuman soft tissue. Indentation experiments were performed on a silicone gelsoft tissue phantom. A two camera DIC setup was then used to record the 3Dsurface deformation. The experiment was then simulated using a FE model.

Evaluation of 99mTc-Label led Vitamin K4 as an Agent for Testicular Imaging

1991 ◽  
Vol 30 (01) ◽  
pp. 35-39 ◽  
Author(s):  
H. S. Durak ◽  
M. Kitapgi ◽  
B. E. Caner ◽  
R. Senekowitsch ◽  
M. T. Ercan
Keyword(s):  
Soft Tissue ◽  
Room Temperature ◽  
Normal Subjects ◽  
Left Testis ◽  
Wide Range ◽  
Activity Ratios ◽  
The Right ◽  
Radioactivity Levels

Vitamin K4 was labelled with 99mTc with an efficiency higher than 97%. The compound was stable up to 24 h at room temperature, and its biodistribution in NMRI mice indicated its in vivo stability. Blood radioactivity levels were high over a wide range. 10% of the injected activity remained in blood after 24 h. Excretion was mostly via kidneys. Only the liver and kidneys concentrated appreciable amounts of radioactivity. Testis/soft tissue ratios were 1.4 and 1.57 at 6 and 24 h, respectively. Testis/blood ratios were lower than 1. In vitro studies with mouse blood indicated that 33.9 ±9.6% of the radioactivity was associated with RBCs; it was washed out almost completely with saline. Protein binding was 28.7 ±6.3% as determined by TCA precipitation. Blood clearance of 99mTc-l<4 in normal subjects showed a slow decrease of radioactivity, reaching a plateau after 16 h at 20% of the injected activity. In scintigraphic images in men the testes could be well visualized. The right/left testis ratio was 1.08 ±0.13. Testis/soft tissue and testis/blood activity ratios were highest at 3 h. These ratios were higher than those obtained with pertechnetate at 20 min post injection.99mTc-l<4 appears to be a promising radiopharmaceutical for the scintigraphic visualization of testes.

scholarly journals Non-invasive diagnostics in fossils - Magnetic Resonance Imaging of pathological belemnites

2005 ◽  
Vol 2 (2) ◽  
pp. 133-140 ◽  
Author(s):  
D. Mietchen ◽  
H. Keupp ◽  
B. Manz ◽  
F. Volke
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Soft Tissue ◽  
Clinical Diagnostics ◽  
Resonance Imaging ◽  
Non Invasive ◽  
Physiological Processes ◽  
Magnetic Resonance Imaging Mri ◽  
Invasive Diagnostics

Abstract. For more than a decade, Magnetic Resonance Imaging (MRI) has been routinely employed in clinical diagnostics because it allows non-invasive studies of anatomical structures and physiological processes in vivo and to differentiate between healthy and pathological states, particularly of soft tissue. Here, we demonstrate that MRI can likewise be applied to fossilized biological samples and help in elucidating paleopathological and paleoecological questions: Five anomalous guards of Jurassic and Cretaceous belemnites are presented along with putative paleopathological diagnoses directly derived from 3D MR images with microscopic resolution. Syn vivo deformities of both the mineralized internal rostrum and the surrounding former soft tissue can be traced back in part to traumatic events of predator-prey-interactions, and partly to parasitism. Besides, evidence is presented that the frequently observed anomalous apical collar might be indicative of an inflammatory disease. These findings highlight the potential of Magnetic Resonance techniques for further paleontological applications.

Predicting in Vivo Soft Tissue Masses of the Lower Extremity Using Segment Anthropometric Measures and DXA

2005 ◽  
Vol 21 (4) ◽  
pp. 371-382 ◽  
Author(s):  
Jeffrey D. Holmes ◽  
David M. Andrews ◽  
Jennifer L. Durkin ◽  
James J. Dowling
Keyword(s):  
Soft Tissue ◽  
Lower Extremity ◽  
Prediction Equation ◽  
Regression Equations ◽  
Anthropometric Measures ◽  
Soft Tissue Masses ◽  
Highly Correlated ◽  
Relative Errors ◽  
Dxa Scans

The purpose of this study was to derive and validate regression equations for the prediction of fat mass (FM), lean mass (LM), wobbling mass (WM), and bone mineral content (BMC) of the thigh, leg, and leg + foot segments of living people from easily measured segmental anthropometric measures. The segment masses of 68 university-age participants (26 M, 42 F) were obtained from full-body dual photon x-ray absorptiometry (DXA) scans, and were used as the criterion values against which predicted masses were compared. Comprehensive anthropometric measures (6 lengths, 6 circumferences, 8 breadths, 4 skinfolds) were taken bilaterally for the thigh and leg for each person. Stepwise multiple linear regression was used to derive a prediction equation for each mass type and segment. Prediction equations exhibited high adjustedR2values in general (0.673 to 0.925), with higher correlations evident for the LM and WM equations than for FM and BMC. Predicted (equations) and measured (DXA) segment LM and WM were also found to be highly correlated (R2= 0.85 to 0.96), and FM and BMC to a lesser extent (R2= 0.49 to 0.78). Relative errors between predicted and measured masses ranged between 0.7% and –11.3% for all those in the validation sample (n= 16). These results on university-age men and women are encouraging and suggest that in vivo estimates of the soft tissue masses of the lower extremity can be made fairly accurately from simple segmental anthropometric measures.

scholarly journals Concomitant control of mechanical properties and degradation in resorbable elastomer-like materials using stereochemistry and stoichiometry for soft tissue engineering

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Mary Beth Wandel ◽  
Craig A. Bell ◽  
Jiayi Yu ◽  
Maria C. Arno ◽  
Nathan Z. Dreger ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Drug Delivery ◽  
Soft Tissue ◽  
Tissue Regeneration ◽  
Biological Tissues ◽  
Soft Tissue Regeneration ◽  
Soft Tissue Engineering ◽  
Degradation Properties ◽  
Enhance Cell Adhesion

AbstractComplex biological tissues are highly viscoelastic and dynamic. Efforts to repair or replace cartilage, tendon, muscle, and vasculature using materials that facilitate repair and regeneration have been ongoing for decades. However, materials that possess the mechanical, chemical, and resorption characteristics necessary to recapitulate these tissues have been difficult to mimic using synthetic resorbable biomaterials. Herein, we report a series of resorbable elastomer-like materials that are compositionally identical and possess varying ratios of cis:trans double bonds in the backbone. These features afford concomitant control over the mechanical and surface eroding degradation properties of these materials. We show the materials can be functionalized post-polymerization with bioactive species and enhance cell adhesion. Furthermore, an in vivo rat model demonstrates that degradation and resorption are dependent on succinate stoichiometry in the elastomers and the results show limited inflammation highlighting their potential for use in soft tissue regeneration and drug delivery.

scholarly journals Improvement of biohistological response of facial implant materials by tantalum surface treatment

2019 ◽  
Vol 41 (1) ◽  
Author(s):  
Mohammed Mousa Bakri ◽  
Sung Ho Lee ◽  
Jong Ho Lee
Keyword(s):  
Foreign Body ◽  
Soft Tissue ◽  
Surface Treatment ◽  
Clinical Applications ◽  
Tissue Response ◽  
Bone Surface ◽  
Tissue Thickness ◽  
Implant Materials ◽  
Soft Tissue Thickness

Abstract Background A compact passive oxide layer can grow on tantalum (Ta). It has been reported that this oxide layer can facilitate bone ingrowth in vivo though the development of bone-like apatite, which promotes hard and soft tissue adhesion. Thus, Ta surface treatment on facial implant materials may improve the tissue response, which could result in less fibrotic encapsulation and make the implant more stable on the bone surface. The purposes of this study were to verify whether surface treatment of facial implant materials using Ta can improve the biohistobiological response and to determine the possibility of potential clinical applications. Methods Two different and commonly used implant materials, silicone and expanded polytetrafluoroethylene (ePTFE), were treated via Ta ion implantation using a Ta sputtering gun. Ta-treated samples were compared with untreated samples using in vitro and in vivo evaluations. Osteoblast (MG-63) and fibroblast (NIH3T3) cell viability with the Ta-treated implant material was assessed, and the tissue response was observed by placing the implants over the rat calvarium (n = 48) for two different lengths of time. Foreign body and inflammatory reactions were observed, and soft tissue thickness between the calvarium and the implant as well as the bone response was measured. Results The treatment of facial implant materials using Ta showed a tendency toward increased fibroblast and osteoblast viability, although this result was not statistically significant. During the in vivo study, both Ta-treated and untreated implants showed similar foreign body reactions. However, the Ta-treated implant materials (silicone and ePTFE) showed a tendency toward better histological features: lower soft tissue thickness between the implant and the underlying calvarium as well as an increase in new bone activity. Conclusion Ta surface treatment using ion implantation on silicone and ePTFE facial implant materials showed the possibility of reducing soft tissue intervention between the calvarium and the implant to make the implant more stable on the bone surface. Although no statistically significant improvement was observed, Ta treatment revealed a tendency toward an improved biohistological response of silicone and ePTFE facial implants. Conclusively, tantalum treatment is beneficial and has the potential for clinical applications.

scholarly journals Frequency-preference response in covalent modification cycles under substrate sequestration conditions

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Juliana Reves Szemere ◽  
Horacio G. Rotstein ◽  
Alejandra C. Ventura
Keyword(s):  
Frequency Response ◽  
Covalent Modification ◽  
Protein Abundance ◽  
Dynamic Features ◽  
Signaling Systems ◽  
Low Pass ◽  
Low Pass Filters ◽  
Periodic Inputs ◽  
Preferred Frequency Response

AbstractCovalent modification cycles (CMCs) are basic units of signaling systems and their properties are well understood. However, their behavior has been mostly characterized in situations where the substrate is in excess over the modifying enzymes. Experimental data on protein abundance suggest that the enzymes and their target proteins are present in comparable concentrations, leading to substrate sequestration by the enzymes. In this enzyme-in-excess regime, CMCs have been shown to exhibit signal termination, the ability of the product to return to a stationary value lower than its peak in response to constant stimulation, while this stimulation is still active, with possible implications for the ability of systems to adapt to environmental inputs. We characterize the conditions leading to signal termination in CMCs in the enzyme-in-excess regime. We also demonstrate that this behavior leads to a preferred frequency response (band-pass filters) when the cycle is subjected to periodic stimulation, whereas the literature reports that CMCs investigated so far behave as low-pass filters. We characterize the relationship between signal termination and the preferred frequency response to periodic inputs and we explore the dynamic mechanism underlying these phenomena. Finally, we describe how the behavior of CMCs is reflected in similar types of responses in the cascades of which they are part. Evidence of protein abundance in vivo shows that enzymes and substrates are present in comparable concentrations, thus suggesting that signal termination and frequency-preference response to periodic inputs are also important dynamic features of cell signaling systems, which have been overlooked.

Vibration Characteristics of Rotating Thin Disks—Part I: Experimental Results

2012 ◽  
Vol 79 (4) ◽  
Author(s):  
Ramin M. H. Khorasany ◽  
Stanley G. Hutton
Keyword(s):  
Frequency Response ◽  
Natural Frequency ◽  
Linear Theory ◽  
Lateral Force ◽  
Vibration Characteristics ◽  
Experimental Results ◽  
Rotating Disks ◽  
Critical Speeds ◽  
Applied Forces ◽  
Thin Disks

Analysis of the linear vibration characteristics of unconstrained rotating isotropic thin disks leads to the important concept of “critical speeds.” These critical rotational speeds are of interest because they correspond to the situation where a natural frequency of the rotating disk, as measured by a stationary observer, is zero. Such speeds correspond physically to the speeds at which a traveling circumferential wave, of shape corresponding to the mode shape of the natural frequency being considered, travel around the disk in the absence of applied forces. At such speeds, according to linear theory, the blade may respond as a space fixed stationary wave and an applied space fixed dc force may induce a resonant condition in the disk response. Thus, in general, linear theory predicts that for rotating disks, with low levels of damping, large responses may be encountered in the region of the critical speeds due to the application of constant space fixed forces. However, large response invalidates the predictions of linear theory which has neglected the nonlinear stiffness produced by the effect of in-plane forces induced by large displacements. In the present paper, experimental studies were conducted in order to measure the frequency response characteristics of rotating disks both in an idling mode as well as when subjected to a space fixed lateral force. The applied lateral force (produced by an air jet) was such as to produce displacements large enough that non linear geometric effects were important in determining the disk frequencies. Experiments were conducted on thin annular disks of different thickness with the inner radius clamped to the driving arbor and the outer radius free. The results of these experiments are presented with an emphasis on recording the effects of geometric nonlinearities on lateral frequency response. In a companion paper (Khorasany and Hutton, 2010, “Vibration Characteristics of Rotating Thin Disks—Part II: Analytical Predictions,” ASME J. Mech., 79(4), p. 041007), analytical predictions of such disk behavior are presented and compared with the experimental results obtained in this study. The experimental results show that in the case where significant disk displacements are induced by a lateral force, the frequency characteristics are significantly influenced by the magnitude of forced displacements.

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