Fiber Filled Hybrid Hydrogel for Bio-Manufacturing

2020 ◽  
pp. 1-38
Author(s):  
MD Habib ◽  
Bashir Khoda
Keyword(s):  
Cell Viability ◽  
Large Scale ◽  
Three Dimensional ◽  
High Water ◽  
Solid Content ◽  
Design Stage ◽  
High Water Content ◽  
Cellulose Derivatives ◽  
Hybrid Hydrogel ◽  
Low Cytotoxicity

Abstract The extrusion based three-dimensional (3D) bio-printing deposits cell-laden bio-ink with high spatial resolution and may offer living tissue regeneration. Due to the biocompatibility, very low cytotoxicity, and high-water content, natural hydrogels are commonly considered as the cell-laden bio-ink for scaffold fabrication. However, due to the low mechanical integrity, a large-scale scaffold (> 10 layers) with intricate architecture is a challenge. In this paper, we developed and characterize a novel bio-ink consisting of alginate, CMC, and TO-NFC for bio-printing applications. The potential of cellulose derivatives in terms of rheological property to satisfy scaffold architecture and cell viability is explored with a relatively small amount of solid content (<5%). By combining alginate, CMC, and TO-NFC as a hybrid hydrogel, we design to overcome their individual challenges as bio-ink. At the design stage, we have considered two main characteristics, printability and shape fidelity with quantitative indices. We studied the rheological characteristics for determining the suitable composition for extrusion bio-printing. Our investigation suggests an optimal material composition that can print 42 layers and a 9 mm tall scaffold structure. The proposed hybrid hydrogel is used to prepare bio-ink encapsulating cells and cell viability is measured as 90% after 10 days of incubation.

Fiber Filled Hybrid Hydrogel for Bio-Manufacturing

2020 ◽  
Author(s):  
Ahasan Habib ◽  
Bashir Khoda
Keyword(s):  
Tissue Regeneration ◽  
Large Scale ◽  
High Spatial Resolution ◽  
Three Dimensional ◽  
High Water ◽  
Solid Content ◽  
High Water Content ◽  
Living Tissue ◽  
Hybrid Hydrogel ◽  
Nano Fiber

Abstract The extrusion based three-dimensional (3D) bio-printing deposits cell-laden bio-ink with high spatial resolution and may offer living tissue regeneration. Due to the biocompatibility, less cytotoxicity and high water content, natural hydrogels are commonly considered as the bio-ink for scaffold fabrication. However, due to the low mechanical integrity, a large scale scaffold (> 10 layers) with intricate architecture is a challenge. In this paper, Cellulose-based nano-fiber and CMC are added with alginate material to improve the rheological behavior of the hybrid hydrogel. Shear-thinning behavior, shape fidelity, printability of the composition are investigated and evaluated for various compositions. Finally, both regular and freeform 3D scaffolds are fabricated with the proposed hybrid hydrogel to validate its printability and shape fidelity. The required properties of bio-ink are highly dependent upon the percentage composition and the solid content.

Ultra-absorbent hybrid hydrogel based on alginate and SiO2 microspheres: A high-water-content system for removal of methylene blue

2019 ◽  
Vol 276 ◽  
pp. 204-213 ◽  
Author(s):  
Caroline O. Panão ◽  
Eduardo L.S. Campos ◽  
Hugo H.C. Lima ◽  
Andrelson W. Rinaldi ◽  
Michele K. Lima-Tenório ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Water Content ◽  
High Water ◽  
High Water Content ◽  
Hybrid Hydrogel ◽  
Content System ◽  
Sio2 Microspheres

scholarly journals Xanthan Gum–Konjac Glucomannan Blend Hydrogel for Wound Healing

Polymers ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 99 ◽  
Author(s):  
Andreia Alves ◽  
Sónia P. Miguel ◽  
André R.T.S. Araujo ◽  
María José de Jesús Valle ◽  
Amparo Sánchez Navarro ◽  
...  
Keyword(s):  
Xanthan Gum ◽  
Biomedical Application ◽  
Three Dimensional ◽  
High Water ◽  
Biological Properties ◽  
Konjac Glucomannan ◽  
Wound Dressings ◽  
Dimensional Structure ◽  
Future Application ◽  
High Water Content

Hydrogels are considered to be the most ideal materials for the production of wound dressings since they display a three-dimensional structure that mimics the native extracellular matrix of skin as well as a high-water content, which confers a moist environment at the wound site. Until now, different polymers have been used, alone or blended, for the production of hydrogels aimed for this biomedical application. From the best of our knowledge, the application of a xanthan gum–konjac glucomannan blend has not been used for the production of wound dressings. Herein, a thermo-reversible hydrogel composed of xanthan gum–konjac glucomannan (at different concentrations (1% and 2% w/v) and ratios (50/50 and 60/40)) was produced and characterized. The obtained data emphasize the excellent physicochemical and biological properties of the produced hydrogels, which are suitable for their future application as wound dressings.

scholarly journals A Customizable, Low-Cost Perfusion System for Sustaining Tissue Constructs

2018 ◽  
Vol 23 (6) ◽  
pp. 592-598
Author(s):  
Brian J. O’Grady ◽  
Jason X. Wang ◽  
Shannon L. Faley ◽  
Daniel A. Balikov ◽  
Ethan S. Lippmann ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Viability ◽  
Large Scale ◽  
Low Cost ◽  
Three Dimensional ◽  
Tissue Scaffolds ◽  
Perfusion System ◽  
Pump System ◽  
Tissue Constructs ◽  
Engineered Tissue

The fabrication of engineered vascularized tissues and organs requiring sustained, controlled perfusion has been facilitated by the development of several pump systems. Currently, researchers in the field of tissue engineering require the use of pump systems that are in general large, expensive, and generically designed. Overall, these pumps often fail to meet the unique demands of perfusing clinically useful tissue constructs. Here, we describe a pumping platform that overcomes these limitations and enables scalable perfusion of large, three-dimensional hydrogels. We demonstrate the ability to perfuse multiple separate channels inside hydrogel slabs using a preprogrammed schedule that dictates pumping speed and time. The use of this pump system to perfuse channels in large-scale engineered tissue scaffolds sustained cell viability over several weeks.

Environmental impacts of a large-scale incinerator with mixed MSW of high water content from a LCA perspective

2015 ◽  
Vol 30 ◽  
pp. 173-179 ◽  
Author(s):  
Ziyang Lou ◽  
Bernd Bilitewski ◽  
Nanwen Zhu ◽  
Xiaoli Chai ◽  
Bing Li ◽  
...  
Keyword(s):  
Water Content ◽  
Environmental Impacts ◽  
Large Scale ◽  
High Water ◽  
High Water Content

scholarly journals Properties and Application of Backfill Materials in Coal Mines in China

Minerals ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 53 ◽  
Author(s):  
Jixiong Zhang ◽  
Meng Li ◽  
Abbas Taheri ◽  
Weiqing Zhang ◽  
Zhongya Wu ◽  
...  
Keyword(s):  
Large Scale ◽  
Coal Mines ◽  
High Stress ◽  
High Water ◽  
High Water Content ◽  
Environmental Aspects ◽  
Coal Resources ◽  
Mining Areas ◽  
Mining Depth ◽  
Backfill Materials

Coal is the basic resource underpinning energy generation in China, however, constant, large-scale mining of coal results in many problems such as ecological destruction of mining areas. As a result, backfilling of solid waste underground is proposed to control strata and surface subsidence and to protect the environment. At present, these materials, such as granular material, cemented material and high-water-content materials are mainly used for backfilling. This study summarised the types of backfill materials that are used in coal mines in China along with the backfilling process. Moreover, distribution and characteristics of mines backfilled with these backfill materials were obtained and analysed. Considering the socio-environmental aspects that affect backfilling, this research proposed a guideline for the selection of backfill materials and then analysed specific engineering cases of three backfill materials. In addition, the future development of backfill materials was discussed. With extensive extraction of shallow coal resources in China and, therefore, rapid depletion of coal resources in eastern regions of China, coal mining depth is increasing significantly. As a result, it is required to investigate new backfill materials suited for the deep high-stress environment.

Rapid Consolidation Behavior of Dredged Clays at High Water Content

2011 ◽  
Vol 368-373 ◽  
pp. 2966-2970
Author(s):  
Yu Peng Cao ◽  
Jian Wen Ding ◽  
Xia Bian ◽  
Feng Ji ◽  
Gui Zhong Xu
Keyword(s):  
Large Scale ◽  
High Water ◽  
Laboratory Model ◽  
High Water Content ◽  
Vertical Drains ◽  
Drainage Rate ◽  
Horizontal Drains ◽  
The Stability ◽  
Stability Time ◽  
Consolidation Behavior

Consolidation of dredged clays with a system of horizontal and vertical drains is considered. Horizontal drains in the system are made of sand layers, while vertical drains are plastic vertical drains (PVD). Laboratory model tests are performed to study the rapid consolidation behavior of layered clay-sand and clay with PVD. Special cares are given to settlement and the drainage rate of dredged clays with radial and vertical drain system. Numerical analysis is also used to identify the stability time of consolidation, and to verify which method is better suitable for handling large-scale dredged clays.

Aqueous two-phase systems as a tool for bioseparation – emphasis on organic acids

2020 ◽  
Vol 5 (9) ◽  
Author(s):  
Dragomir Yankov
Keyword(s):  
Organic Acids ◽  
Protein Separation ◽  
Large Scale ◽  
Process Integration ◽  
Scale Up ◽  
High Water ◽  
High Water Content ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

AbstractAqueous two-phase systems (ATPS) are universally recognized as an excellent alternative to the conventional separation techniques in the biotechnology, because of their undoubted advantages such as mild and biocompatible conditions, high water content, low interfacial tension, ease of process integration and scale up, etc. The formation of ATPS is due to the incompatibility of two polymers in a common solution. Other types of ATPS are formed by polymer/salt, ionic and/or non-ionic surfactants, inorganic salt/short-chain alcohols, and based on room temperature ionic liquids. ATPS are successfully used (even in large scale) for cells, enzyme and protein separation, while their application for recovery of small molecules such as organic acids, antibiotics, alcohols is more complicated as they are usually hydrophilic and tend to distribute evenly between the phases. The purpose of this paper is to overview and summarize the efforts made for the application of different types of ATPS for the separation of organic acids.

Application of Vibrating Fluidized Bed in Low Rank Coal Drying Process

2013 ◽  
Vol 788 ◽  
pp. 233-236
Author(s):  
Yong Jie Zhao ◽  
Xiang Wei Kong ◽  
Bin Li ◽  
Zi Cheng Zhang
Keyword(s):  
Fluidized Bed ◽  
High Speed ◽  
Large Scale ◽  
Energy Resource ◽  
High Water ◽  
Low Rank ◽  
High Water Content ◽  
Drying Process ◽  
Low Rank Coal ◽  
Coal Drying

Low rank coal is an important energy resource in the world, particularly in the developing countries. But its high water content causes a waste of energy and serious environmental pollution, which restricts its applications. In order to improve the efficiency of the low rank coal and reduce pollution, the drying processes for low rank coal are urgently needed. This paper presented a review of the general drying processes for low rank coal. In the review, the newly-developed vibrating fluidized bed (VFB) drying process was emphasized. This novel VFB drying process which can be used in large-scale industrial production has several advantages, such as high speed, high drying intensity and low pollution. Moreover, two VFB researches reported by the authors were also introduced. In these researches, the dynamic behavior of VFB was simulated by two commercial codes of ADAMS and ANSYS separately. According to the simulation results, the exciting force and the structure of sieve box were optimized.

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