High Speed Particles Separation Using Ultrasound for Lab-on-Chip Application

2004 ◽  
Author(s):  
Holden Li ◽  
Vipin Vitikkate ◽  
Thomas Kenny
Keyword(s):  
Human Blood ◽  
Blood Cells ◽  
High Speed ◽  
Point Of Care ◽  
White Blood Cells ◽  
Small Sample ◽  
Separation Mechanism ◽  
Lab On Chip ◽  
Micro Particle Image Velocimetry ◽  
On Chip

Engineers have long envisioned that a handheld portable blood diagnosis device would be able to give an accurate measurement of chemical content based on a very small sample in the shortest time possible. One of the immediate applications of such device is the Point Of Care (POC) diagnosis system, whereby a single drop of human blood would determine his health status. However, a major technical challenge lies in the ability to separate different particles, which in the case of human blood, is to separate red and white blood cells and plasma in a quick, cheap, reliable device with low power consumption (less than 100mW). In this paper, we present some preliminary results from our tests of ultrasound standing waves as a potential separation mechanism for blood cells. Micro Particle Image Velocimetry (PIV) technique is the studies.

DETERMINATION OF WHITE BLOOD CELLS USING FOLDSCOPE WITH SMARTPHONE

2019 ◽  
pp. 10-13
Author(s):  
Ranu Kumar ◽  
Prasad Kapildeo
Keyword(s):  
Human Blood ◽  
Blood Cells ◽  
Blood Smear ◽  
Clinical Laboratory ◽  
White Blood Cells ◽  
Healthcare Services ◽  
Field Level ◽  
Morphology Analysis

We are traditionally used Microscope in clinical laboratory for determination of white blood cells of human blood smear. Now, in this study we were used Foldscope with Smartphone in the place of Microscope and examine many samples of human blood smear which was collected from local diagnostic centers. We were very easily quantity & morphology analysis of all types of WBC cells such as Neutrophils, Lymphocytes, Monocytes, Eosionophils, Basophils in blood smear with the help of Foldscope & image taken by Smartphone. The main objective of this study is to use Foldscope for quantity & morphology analysis of human WBCs at field level especially poor resource area where healthcare services or centers is not available & where carry of microscope is not possible.

scholarly journals Direct and rapid measurement of hydrogen peroxide in human blood using a microfluidic device

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
R. Gaikwad ◽  
P. R. Thangaraj ◽  
A. K. Sen
Keyword(s):  
Hydrogen Peroxide ◽  
Microfluidic Device ◽  
Human Blood ◽  
Blood Cells ◽  
Limit Of Detection ◽  
Point Of Care ◽  
Time Interval ◽  
Fluorescence Measurement ◽  
Rapid Measurement ◽  
Automated Method

AbstractThe levels of hydrogen peroxide ($${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$ H 2 O 2 ) in human blood is of great relevance as it has emerged as an important signalling molecule in a variety of disease states. Fast and reliable measurement of $${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$ H 2 O 2 levels in the blood, however, continues to remain a challenge. Herein we report an automated method employing a microfluidic device for direct and rapid measurement of $${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$ H 2 O 2 in human blood based on laser-induced fluorescence measurement. Our study delineates the critical factors that affect measurement accuracy—we found blood cells and soluble proteins significantly alter the native $${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$ H 2 O 2 levels in the time interval between sample withdrawal and detection. We show that separation of blood cells and subsequent dilution of the plasma with a buffer at a ratio of 1:6 inhibits the above effect, leading to reliable measurements. We demonstrate rapid measurement of $${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$ H 2 O 2 in plasma in the concentration range of 0–49 µM, offering a limit of detection of 0.05 µM, a sensitivity of 0.60 µM−1, and detection time of 15 min; the device is amenable to the real-time measurement of $${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$ H 2 O 2 in the patient’s blood. Using the linear correlation obtained with known quantities of $${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$ H 2 O 2 , the endogenous $${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$ H 2 O 2 concentration in the blood of healthy individuals is found to be in the range of 0.8–6 µM. The availability of this device at the point of care will have relevance in understanding the role of $${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$ H 2 O 2 in health and disease.

Point-of-Care Systems for Rapid DNA Quantification in Oncology

2008 ◽  
Vol 94 (2) ◽  
pp. 216-225 ◽  
Author(s):  
Marco Bianchessi ◽  
Sarah Burgarella ◽  
Marco Cereda
Keyword(s):  
Point Of Care ◽  
Low Cost ◽  
Semiconductor Industry ◽  
Point Of Care Testing ◽  
Lab On Chip ◽  
Diagnostic Assays ◽  
Care Systems ◽  
Point Of Care Systems ◽  
The Common ◽  
On Chip

The development of new powerful applications and the improvement in fabrication techniques are promising an explosive growth in lab-on-chip use in the upcoming future. As the demand reaches significant levels, the semiconductor industry may enter in the field, bringing its capability to produce complex devices in large volumes, high quality and low cost. The lab-on-chip concept, when applied to medicine, leads to the point-of-care concept, where simple, compact and cheap instruments allow diagnostic assays to be performed quickly by untrained personnel directly at the patient's side. In this paper, some practical and economical considerations are made to support the advantages of point-of-care testing. A series of promising technologies developed by STMicroelectronics on lab-on-chips is also presented, mature enough to enter in the common medical practice. The possible use of these techniques for cancer research, diagnosis and treatment are illustrated together with the benefits offered by their implementation in point-of-care testing.

scholarly journals Plasma skimming efficiency of human blood in the spiral groove bearing of a centrifugal blood pump

2020 ◽  
Author(s):  
Daisuke Sakota ◽  
Kazuki Kondo ◽  
Ryo Kosaka ◽  
Masahiro Nishida ◽  
Osamu Maruyama
Keyword(s):  
Red Blood Cells ◽  
Human Blood ◽  
Blood Cells ◽  
High Speed ◽  
Blood Pump ◽  
Spiral Groove ◽  
Centrifugal Blood Pump ◽  
Plasma Skimming ◽  
Ridge Area ◽  
Spiral Groove Bearing

Abstract This work investigates the plasma skimming effect in a spiral groove bearing within a hydrodynamically levitated centrifugal blood pump when working with human blood having a hematocrit value from 0 to 40%. The present study assessed the evaluation based on a method that clarified the limitations associated with such assessments. Human blood was circulated in a closed-loop circuit via a pump operating at 4000 rpm at a flow rate of 5 L/min. Red blood cells flowing through a ridge area of the bearing were directly observed using a high-speed microscope. The hematocrit value in the ridge area was calculated using the mean corpuscular volume, the bearing gap, the cross-sectional area of a red blood cell, and the occupancy of red blood cells. The latter value was obtained from photographic images by dividing the number of pixels showing red blood cells in the evaluation area by the total number of pixels in this area. The plasma skimming efficiency was calculated as the extent to which the hematocrit of the working blood was reduced in the ridge area. For the hematocrit in the circuit from 0 to 40%, the plasma skimming efficiency was approximately 90%, meaning that the hematocrit in the ridge area became 10% as compared to that in the circuit. For a hematocrit of 20% and over, red blood cells almost completely occupied the ridge. Thus, a valid assessment of plasma skimming was only possible when the hematocrit was less than 20%.

Electrothermal Blinking Vortices for Chaotic Mixing

2012 ◽  
Author(s):  
Sophie Loire ◽  
Paul Kauffmann ◽  
Paul Gimenez ◽  
Igor Mezić ◽  
Carl Meinhart
Keyword(s):  
Step Function ◽  
Time Dependent ◽  
Chaotic Mixing ◽  
Electric Signal ◽  
Dynamical System Theory ◽  
Mixing Efficiency ◽  
Lab On Chip ◽  
Micro Particle Image Velocimetry ◽  
Phase Signal ◽  
On Chip

Thanks to its favorable reduction scale law, and its easy integration, electrokinetics has emerged over the last fifteen years as one of the major solution to drive flows in fully integrated lab-on-chip. At microscale, an efficient mixing is a keystep which can dramatically accelerate bio-reactions. For thirty years, Dynamical System theory has predicted that chaotic mixing must involve at least 3 dimensions (either time dependent 2D flows or 3D flows). However, in microfluidics, few works have yet presented efficient embedded micromixers. This paper presents experimental and theoretical study of 2D time dependent chaotic mixing using AC electrothermal fluid flows. Experiments and numerical simulations are performed on a top view device and a sideview device. In both devices, a sinusoidal electric signal is applied between 3 interdigitated gold electrodes. A phase signal Vpp = 11V and a ground are switched between the two side electrodes using a step function, whereas the opposite phase signal –Vpp is steadily applied to the center electrode (Figure 1). Flow velocity is measured by micro particle image velocimetry μ PIV. The velocity profile shows a dramatic asymmetry between the two vortices. Therefore, during the switch, vortices overlap, leading to stretching and folding flows required to obtain chaotic mixing (Figure 3 and 4). The experimental measurements validate our electrothermal models based on our previous work [1]. The mixing efficiency of low diffusive particles is studied at multiscale using the mix-variance coefficient (MVC) [2] to evaluate mixing at different scales (Figure 4). To do so, the domain is successively divided in boxes along the x and y direction up to nx and ny boxes, respectively. For each box configuration, average bead concentration is computed. The variance of these concentrations is then evaluated: MVCs=1nxny∑i=1ny∑j=1nxρij-0.52. The result of numerically evaluated MVC in Figure 2 show a dramatic increase of mixing efficiency with blinking vortices compared to steady flow. Theoretical, experimental and simulation results of the mixing process will be presented.

scholarly journals On-chip Extraction of Intracellular Molecules in White Blood Cells from Whole Blood

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Jongchan Choi ◽  
Ji-chul Hyun ◽  
Sung Yang
Keyword(s):  
Whole Blood ◽  
Blood Cells ◽  
White Blood Cells ◽  
On Chip

SENSING AND ACTUATING BIO-MATERIALS ON STANDARD CMOS SUBSTRATES

2013 ◽  
Vol 22 (02) ◽  
pp. 1250081 ◽  
Author(s):  
FAISAL T. ABU-NIMEH ◽  
FATHI M. SALEM
Keyword(s):  
High Speed ◽  
High Efficiency ◽  
Low Cost ◽  
Magnetic Bead ◽  
Column Switching ◽  
Parallel Operation ◽  
Coil Array ◽  
Lab On Chip ◽  
On Chip ◽  
Array Sensing

We present a low-cost, low-power, high efficiency, and portable integrated implementations of a lab-on-chip for magnetic molecular level sensing manipulation, and diagnosis. The design features an all-integrated programmable magnetic coil array for sensing and actuating small magnetic bead objects. The coil array is selectively and dynamically controlled using the smallest permissible vertical coil inductors in this technology. Each cell, composed of the coil and its logical control circuitry, can detect small objects in the order of 1 μm diameter as well as emit eight programmable magnetic field levels for manipulation. All array sensing and driving components are shared to reduce the overall imprint. They are tuned towards the 900 s MHz ISM band and incorporating high-speed serial row/column switching up to 40 MHz for seamless pseudo-parallel operation.

Rapid and portable, lab-on-chip, point-of-care genotyping for evaluating clopidogrel metabolism

2015 ◽  
Vol 451 ◽  
pp. 240-246 ◽  
Author(s):  
Nicola Marziliano ◽  
Maria Francesca Notarangelo ◽  
Marco Cereda ◽  
Vittoria Caporale ◽  
Lucia Coppini ◽  
...  
Keyword(s):  
Point Of Care ◽  
Lab On Chip ◽  
On Chip

scholarly journals Fast, accurate, point-of-care COVID-19 pandemic diagnosis enabled through advanced lab-on-chip optical biosensors: Opportunities and challenges

2021 ◽  
Vol 8 (3) ◽  
pp. 031313
Author(s):  
Aref Asghari ◽  
Chao Wang ◽  
Kyoung Min Yoo ◽  
Ali Rostamian ◽  
Xiaochuan Xu ◽  
...  
Keyword(s):  
Point Of Care ◽  
Optical Biosensors ◽  
Lab On Chip ◽  
On Chip

scholarly journals A review on lab-on-chip as a potential diagnostic tool for early detection of Plasmodium knowlesi

2020 ◽  
Vol 4 (9) ◽  
Author(s):  
Solihah Maketar ◽  
Nurhidanatasha Abu Bakar
Keyword(s):  
Early Detection ◽  
Diagnostic Tool ◽  
Malaria Infection ◽  
High Speed ◽  
Plasmodium Knowlesi ◽  
Acute Infection ◽  
Diagnostic Methods ◽  
Lab On Chip ◽  
Advantages And Disadvantages ◽  
On Chip

Massive elimination efforts have been done to control the malaria disease caused by the emergence of the fifth human malaria parasite known as Plasmodium knowlesi. Early detection of the parasite is important in treating malaria infection. Microscopic examination of Giemsa-stained thick and thin blood films is the gold standard for laboratory malaria diagnosis, while rapid diagnostic tests (RDTs), polymerase chain reaction (PCR) and loop-mediated isothermal amplification (LAMP) are significant diagnostic techniques to detect acute infection. However, these methods have several limitations in which it could delay the treatment. The potential of lab-on-chip (LOC) as a point-of-care diagnostic tool for malaria fulfils the requirement of limitations where it is able to produce early detection of malaria infection. This review discusses advantages and disadvantages of malaria diagnostic methods as well as new approaches that could be used for high speed, sensitive and reliable malaria detection to prevent the disease from causing severe complications and even fatal if left untreated.

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