Advanced Biomimetic Nanodevice Using Nanotechnology Addressable Lipid Rafts Nanoarrays Toward Advanced Nanomaterials

2013 ◽  
Author(s):  
HeaYeon Lee ◽  
JuKyung Lee
Keyword(s):  
Lipid Rafts ◽  
Lipid Bilayer ◽  
Single Molecule ◽  
Signal To Noise Ratio ◽  
High Sensitivity ◽  
Clinical Diagnostics ◽  
New Paradigm ◽  
Signal To Noise ◽  
Critical Issues ◽  
Single Molecule Analysis

In recent years, a new paradigm of nanobiomedical devices combining miniaturization and integration has been exploited in areas such as combinational chemistry, biotechnology, engineering, proteomics and clinical diagnostics. One of the critical issues in the development of nanobiomedical system is how to differentiate signal-to-noise ratio per very small amount of signal. Biocompatible integrated nanopattern requires the fabrication of appropriately designed nanomatrix for high sensitivity homogenous assays, which are capable of ultimately mimic the physiological environment. We reported the nanomatrix geometry of a well-oriented nanowell array derived from nanofabrication technology which can easily be employed for digital detection with a high S/N ratio, miniaturization, integrated assays and single molecule analysis. In this present, we describe a nano(submicro) array of tethered lipid bilayer raft membranes comprising a biosensing platform.

scholarly journals Single-molecule optical absorption imaging by nanomechanical photothermal sensing

2018 ◽  
Vol 115 (44) ◽  
pp. 11150-11155 ◽  
Author(s):  
Miao-Hsuan Chien ◽  
Mario Brameshuber ◽  
Benedikt K. Rossboth ◽  
Gerhard J. Schütz ◽  
Silvan Schmid
Keyword(s):  
Single Molecule ◽  
Shot Noise ◽  
Signal To Noise Ratio ◽  
Local Heating ◽  
Single Molecules ◽  
High Sensitivity ◽  
Temperature Sensitive ◽  
Signal To Noise ◽  
Promising Alternative ◽  
Noise Ratio

Absorption microscopy is a promising alternative to fluorescence microscopy for single-molecule imaging. So far, molecular absorption has been probed optically via the attenuation of a probing laser or via photothermal effects. The sensitivity of optical probing is not only restricted by background scattering but it is fundamentally limited by laser shot noise, which minimizes the achievable single-molecule signal-to-noise ratio. Here, we present nanomechanical photothermal microscopy, which overcomes the scattering and shot-noise limit by detecting the photothermal heating of the sample directly with a temperature-sensitive substrate. We use nanomechanical silicon nitride drums, whose resonant frequency detunes with local heating. Individual Au nanoparticles with diameters from 10 to 200 nm and single molecules (Atto 633) are scanned with a heating laser with a peak irradiance of 354 ± 45 µW/µm2 using 50× long-working-distance objective. With a stress-optimized drum we reach a sensitivity of 16 fW/Hz1/2 at room temperature, resulting in a single-molecule signal-to-noise ratio of >70. The high sensitivity combined with the inherent wavelength independence of the nanomechanical sensor presents a competitive alternative to established tools for the analysis and localization of nonfluorescent single molecules and nanoparticles.

scholarly journals Next-Generation Sequencing: From Basic Research to Diagnostics

2009 ◽  
Vol 55 (4) ◽  
pp. 641-658 ◽  
Author(s):  
Karl V Voelkerding ◽  
Shale A Dames ◽  
Jacob D Durtschi
Keyword(s):  
Next Generation Sequencing ◽  
Dna Sequencing ◽  
Single Molecule ◽  
Basic Research ◽  
Clinical Diagnostics ◽  
Massively Parallel ◽  
Next Generation ◽  
New Paradigm ◽  
The Impact ◽  
Generation Sequencing

Abstract Background: For the past 30 years, the Sanger method has been the dominant approach and gold standard for DNA sequencing. The commercial launch of the first massively parallel pyrosequencing platform in 2005 ushered in the new era of high-throughput genomic analysis now referred to as next-generation sequencing (NGS). Content: This review describes fundamental principles of commercially available NGS platforms. Although the platforms differ in their engineering configurations and sequencing chemistries, they share a technical paradigm in that sequencing of spatially separated, clonally amplified DNA templates or single DNA molecules is performed in a flow cell in a massively parallel manner. Through iterative cycles of polymerase-mediated nucleotide extensions or, in one approach, through successive oligonucleotide ligations, sequence outputs in the range of hundreds of megabases to gigabases are now obtained routinely. Highlighted in this review are the impact of NGS on basic research, bioinformatics considerations, and translation of this technology into clinical diagnostics. Also presented is a view into future technologies, including real-time single-molecule DNA sequencing and nanopore-based sequencing. Summary: In the relatively short time frame since 2005, NGS has fundamentally altered genomics research and allowed investigators to conduct experiments that were previously not technically feasible or affordable. The various technologies that constitute this new paradigm continue to evolve, and further improvements in technology robustness and process streamlining will pave the path for translation into clinical diagnostics.

scholarly journals Scanning single-molecule counting system for Eprobe with highly simple and effective approach

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0243319
Author(s):  
Takeshi Hanami ◽  
Tetsuya Tanabe ◽  
Takuya Hanashi ◽  
Mitsushiro Yamaguchi ◽  
Hidetaka Nakata ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Single Molecule ◽  
Detection System ◽  
Signal To Noise Ratio ◽  
High Sensitivity ◽  
Nucleic Acid Detection ◽  
Digital Measurement ◽  
Excitation Light ◽  
Target Nucleic Acid ◽  
Fluorescent Molecules

Here, we report a rapid and ultra-sensitive detection technique for fluorescent molecules called scanning single molecular counting (SSMC). The method uses a fluorescence-based digital measurement system to count single molecules in a solution. In this technique, noise is reduced by conforming the signal shape to the intensity distribution of the excitation light via a circular scan of the confocal region. This simple technique allows the fluorescent molecules to freely diffuse into the solution through the confocal region and be counted one by one and does not require statistical analysis. Using this technique, 28 to 62 aM fluorescent dye was detected through measurement for 600 s. Furthermore, we achieved a good signal-to-noise ratio (S/N = 2326) under the condition of 100 pM target nucleic acid by only mixing a hybridization-sensitive fluorescent probe, called Eprobe, into the target oligonucleotide solution. Combination of SSMC and Eprobe provides a simple, rapid, amplification-free, and high-sensitive target nucleic acid detection system. This method is promising for future applications to detect particularly difficult to design primers for amplification as miRNAs and other short oligo nucleotide biomarkers by only hybridization with high sensitivity.

scholarly journals High-Sensitivity Large-Area Photodiode Read-Out Using a Divide-and-Conquer Technique

Sensors ◽  
2020 ◽  
Vol 20 (21) ◽  
pp. 6316
Author(s):  
Guillermo Royo ◽  
Carlos Sánchez-Azqueta ◽  
Concepción Aldea ◽  
Santiago Celma
Keyword(s):  
Traditional Approach ◽  
Signal To Noise Ratio ◽  
High Sensitivity ◽  
Divide And Conquer ◽  
Transimpedance Amplifier ◽  
Large Area ◽  
Signal To Noise ◽  
Remarkable Improvement ◽  
The Core ◽  
Novel Technique

In this letter, we present a novel technique to increase the sensitivity of optical read-out with large integrated photodiodes (PD). It consists of manufacturing the PD in several pieces, instead of a single device, and connecting a dedicated transimpedance amplifier (TIA) to each of these pieces. The output signals of the TIAs are combined, achieving a higher signal-to-noise ratio than with the traditional approach. This work shows a remarkable improvement in the sensitivity and transimpedance without the need for additional modifications or compensation techniques. As a result, an increase in sensitivity of 7.9 dBm and transimpedance of 8.7 dBΩ for the same bandwidth is achieved when dividing the photodiode read-out into 16 parallel paths. The proposed divide-and-conquer technique can be applied to any TIA design, and it is also independent of the core amplifier structure and fabrication process, which means it is compatible with every technology allowing the integration of PDs.

A Carbon-Based DNA Framework Nano–Bio Interface for Biosensing with High Sensitivity and a High Signal-to-Noise Ratio

ACS Sensors ◽  
2020 ◽  
Vol 5 (12) ◽  
pp. 3979-3987
Author(s):  
Jing Su ◽  
Wenhan Liu ◽  
Shixing Chen ◽  
Wangping Deng ◽  
Yanzhi Dou ◽  
...  
Keyword(s):  
Signal To Noise Ratio ◽  
High Sensitivity ◽  
High Signal ◽  
Signal To Noise ◽  
Noise Ratio ◽  
Carbon Based

scholarly journals Cooperative GPS and Neighbors Awareness Based Device Discovery for D2D Communication in in-Band Cellular Networks

2018 ◽  
Vol 7 (2.29) ◽  
pp. 700 ◽  
Author(s):  
O Hayat ◽  
R Ngah ◽  
Yasser Zahedi
Keyword(s):  
Cellular Networks ◽  
Channel Capacity ◽  
Signal To Noise Ratio ◽  
Threshold Value ◽  
D2d Communication ◽  
Core Network ◽  
New Paradigm ◽  
Signal To Noise ◽  
Device Discovery ◽  
Noise Ratio

Device to Device (D2D) communication is a new paradigm for next-generation wireless systems to offload data traffic. A device needs to discover neighbor devices on the certain channel to initiate the D2D communication within the minimum period. A device discovery technique based on Global Positioning System (GPS) and neighbor awareness base are proposed for in-band cellular networks. This method is called network-centric approach, and it improves the device discovery efficiency, accuracy, and channel capacity. The differential code is applied to measure the signal to noise ratio of each discovered device. In the case that the signal to noise ratio (SNR) of two devices is above a specified threshold value, then these two devices are qualified for D2D communication. Two procedures are explored for device discovery; discovery by CN (core network) and eNB (evolved node B) cooperation with the help of GPS and neighbor awareness. Using ‘Haversine’ formula, SNR base distance is calculated. Results show an increment in the channel capacity relative to SNR obtained for each device.  

Performance Data of a New 2048 X 2048 Pixel Slow-Scan CCD Camera For TEM

2000 ◽  
Vol 6 (S2) ◽  
pp. 732-733
Author(s):  
S.A. Hiller ◽  
B. Kabius ◽  
W. Probst ◽  
H. Tröster ◽  
M. Trendelenburg ◽  
...  
Keyword(s):  
Low Dose ◽  
Signal To Noise Ratio ◽  
Ccd Camera ◽  
High Sensitivity ◽  
Signal To Noise ◽  
Ideal Image ◽  
Main Disadvantage ◽  
Ccd Arrays ◽  
Holographic Reconstruction ◽  
The Ideal

Excellent linearity and high sensitivity have made SSCs the ideal image detector for almost every TEM application. Their ability to make high quality digital images available within fraction of seconds for further evaluation and processing in a PC, have made them a non-dispensable accessory for any modern TEM. However, despite their excellent characteristics, SSCs provide a restricted number of individual image points in respect to a negative, what is considered to be the main disadvantage of this detector. To compensate for this, CCDs with 2048x2048 pixel are available since some time. SSCs using these 2kx2k CCD arrays not only provide 4 times the pixel number but also offer a lot more options people have waiting for: e. g. highly resolved low-dose or ESI images with significantly improved signal to noise ratio, or higher resolved images for diffraction analysis and holographic reconstruction.

scholarly journals Shielding of Piezoelectric Ultrasonic Probes in Hall Effect Imaging

1998 ◽  
Vol 20 (3) ◽  
pp. 206-220 ◽  
Author(s):  
Han Wen ◽  
Eric Bennett ◽  
David G. Wiesler
Keyword(s):  
Hall Effect ◽  
Electromagnetic Interference ◽  
Thermal Noise ◽  
Signal To Noise Ratio ◽  
High Sensitivity ◽  
Excitation Pulse ◽  
Signal To Noise ◽  
Coherent Noise ◽  
Electrical Pulses ◽  
Coupling Mechanisms

This paper addresses significant sources of electromagnetic noise in Hall effect imaging. Hall effect imaging employs large electrical pulses for signal generation and high sensitivity ultrasonic probes for signal reception. Coherent noise arises through various coupling mechanisms between the excitation pulse and the probe. In this paper, the coupling mechanisms are experimentally isolated and theoretically analyzed. Several methods of shielding the probe from electromagnetic interference are devised and tested. These methods are able to reduce the noise to levels below the random thermal noise, thereby improving the signal-to-noise ratio in HEI by two orders of magnitude.

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