The Effect of Storing Temperature and Duration on Urinary Hydration Markers

2017 ◽  
Vol 27 (1) ◽  
pp. 18-24 ◽  
Author(s):  
J.D. Adams ◽  
Stavros A. Kavouras ◽  
Evan C. Johnson ◽  
Lisa T. Jansen ◽  
Catalina Capitan-Jimenez ◽  
...  
Keyword(s):  
Specific Gravity ◽  
Urine Sample ◽  
Human Urine ◽  
Room Temperature ◽  
Storage Temperature ◽  
Urine Specific Gravity ◽  
Urinary Sediment ◽  
C Storage ◽  
Urine Color ◽  
Urine Specimens

The purpose of this investigation was to quantify the effects of storage temperature, duration, and the urinary sediment on urinary hydration markers. Thirty-six human urine samples were analyzed fresh and then the remaining sample was separated into 24 separate vials, six in each of the following four temperatures: 22 °C, 7 °C, -20 °C, and -80 °C. Two of each sample stored in any given temperature, were analyzed after 1, 2, and 7 days either following vortexing or centrifugation. Each urine sample was analyzed for osmolality (UOsm), urine specific gravity (USG), and urine color (UC). UOsm was stable at 22 °C, for 1 day (+5–9 mmol∙kg-1, p > .05) and at 7 °C, UOsm up to 7 days (+8–8 mmol∙kg-1, p > .05). At -20 and -80 °C, UOsm decreased after 1, 2, and 7 days (9–61 mmol∙kg-1, p < .05). Vortexing the sample before analysis further decreased only UOsm in the -20 °C and -80 °C storage. USG remained stable up to 7 days when samples were stored in 22 °C or 7 °C (p > .05) but declined significantly when stored in -20 °C, and -80 °C (p < .001). UC was not stable in any of the storing conditions for 1, 2, and 7 days. In conclusion, these data indicate that urine specimens analyzed for UOsm or USG remained stable in refrigerated (7 °C) environment for up to 7 days, and in room temperature for 1 day. However, freezing (-20 and -80 °C) samples significantly decreased the values of hydration markers.

scholarly journals Evaluasi Penyimpanan Spermatozoa Ayam Pada Suhu 5⁰C, 26⁰C Dengan Pengencer Infuse NaCl, Glukosa 5% dan 10%

2021 ◽  
pp. 10-19
Author(s):  
Asnawi Asnawi ◽  
Maskur Maskur ◽  
Adji Santoso Dradjat
Keyword(s):  
Cold Storage ◽  
Room Temperature ◽  
Storage Temperature ◽  
Room Temperature Storage ◽  
C Storage ◽  
Better Than ◽  
Temperature Storage

The purpose of this study were to compare the quality of spermatozoa stored at 26⁰C, 5⁰C using diluents of NaCl, 10% glucose and 5% glucose. The spermatozoa of a rooster was collected and divided into 6 parts, each 2 tubes diluted in a ratio of 1:1 using NaCl, Glucose5% and Glucose 10%, then each 3 tubes with different diluents were stored at 26⁰C and 5⁰C. Observations of motility, viability and abnormalities of spermatozoa were carried out half an hour, 1 hour after dilution, followed every 2 hours until the ninth hours. The results showed that spermatozoa stored for 9 hours at a temperature of 26⁰C with a physiological diluent of NaCl, 10% Glucose and 5% Glucose each were different (P, < 0.05) with motility 50 ± 0.0%, 42 ± 10.95. % and 34±8.94%, respectively. At storage temperature of 5⁰C for 9 hours, physiological NaCl, 10% glucose and 5% glucose were significantly different (P<0.05) with motility 58.00±10.95%, 46.00±8.94% and 38.00±, respectively. 10.95% in a row. The viability of spermatozoa at 26⁰C storage with 5% glucose diluent was better than 10% glucose and physiological NaCl (P<0.05), 58.93±1.27%, 42.93±1.48% and 33.43±1.27% , while the physiological NaCl diluent and 10% glucose were not significantly different (P>0.05). At 5⁰C storage the viability of spermatozoa in the three diluents was not significantly different, with values of Glucose 10%, Glucose 5% and physiological NaCl 52.57±5.15%, 52.21±5.02% and 48.14±8.09%, respectively. Spermatozoa abnormalities at storage temperature 26⁰C and 5⁰C for 9 hours using physiological NaCl diluent, 5% glucose and 10% glucose, were not significantly different and varied between 5 to 10%. Finally, it can be concluded that at room temperature storage less than 4 hours the quality of spermatozoa was better with 5% glucose diluent, while for cold storage beyond 4 hours the quality of spermatozoa with NaCl diluent was higher

SOLVOLYSABLE DEOXYCORTICOSTERONE CONJUGATES IN HUMAN URINE

1976 ◽  
Vol 68 (2) ◽  
pp. 273-281 ◽  
Author(s):  
C. L. COPE ◽  
S. LOIZOU
Keyword(s):  
Human Urine ◽  
Room Temperature ◽  
Urine Samples ◽  
Variable Ratio ◽  
The Mean ◽  
Sulphate Conjugate ◽  
Urine Specimens

SUMMARY The nature of the urinary conjugate converted by solvolysis, to free unconjugated deoxycorticosterone (DOC) was studied. A comparison of 11 solvolysis techniques has shown that the method employed in this study yielded 86% of the highest yield by any of the techniques tried. Three successive chromatographic systems on paper showed that no appreciable amounts of contaminants were present in the free DOC eluates, following solvolysis. By preparing authentic [3H]DOC sulphate and subjecting it to solvolysis it was shown that more than 90% of the tritiated DOC was recovered, after chromatography of the free DOC extract. This suggests that much of the solvolysable DOC in human urine is present in the form of the sulphate conjugate. The levels of DOC, excreted as the solvolysable conjugate in a variety of urine specimens, were shown to be much higher than those of free DOC, the former being 4·8 to 127 times higher than the amount of the latter. This highly variable ratio suggests that the site of production of solvolysable DOC is different from that for free DOC. The only correlation between free and solvolysable DOC was shown in dexamethasone-suppressed patients, in whom the mean percentage remaining after suppression was 30·6% for free DOC, 24·1% for solvolysable DOC and 22·2% for cortisol. As solvolysable DOC is present in much larger amounts in urine, care is necessary in the storage of urine samples in which free DOC estimates are to be made, as we found that urine specimens left at room temperature for 1 week could show rises of as much as 400% of their starting free DOC levels.

scholarly journals Effects of sex and menstrual cycle on volume-regulatory responses to 24-h fluid restriction

2020 ◽  
Vol 319 (5) ◽  
pp. R560-R565
Author(s):  
Gabrielle E. W. Giersch ◽  
Abigail T. Colburn ◽  
Margaret C. Morrissey ◽  
Cody R. Butler ◽  
Michaela L. Pruchnicki ◽  
...  
Keyword(s):  
Menstrual Cycle ◽  
Specific Gravity ◽  
Body Mass ◽  
Urine Osmolality ◽  
Reproductive Hormones ◽  
Urine Specific Gravity ◽  
Fluid Restriction ◽  
Urine Color ◽  
Fluid Regulation ◽  
The Impact

Reproductive hormones have significant nonreproductive physiological effects, including altering fluid regulation. Our purpose was to explore the impact of sex and menstrual cycle (MC) phase on volume-regulatory responses to 24-h fluid restriction (24-h FR). Participants (men: n = 12, 20 ± 2 yr; women: n = 10, 20 ± 1 yr) were assigned two randomized and counterbalanced fluid prescriptions [Euhy: euhydrated, urine specific gravity (USG) < 1.020; Dehy: 24-h FR, USG > 1.020]. Men completed both (MEuhy, MDehy), while women completed both in the late-follicular ( days 10–13; FDehy, FEuhy) and midluteal ( days 18–22; LDehy, LEuhy) phases. We measured body mass, plasma and urine osmolality (Posm, Uosm), urine specific gravity (USG), urine color (Ucol), and serum copeptin; 24-h FR yielded mild dehydration without influence of sex or MC ( P > 0.05). Copeptin increased in men following Dehy (pre: 8.2 ± 5.2, post: 15.8 ± 12.6, P = 0.04) but not in women (FDehy pre: 4.3 ± 1.6, post: 10.5 ± 6.9, P = 0.06; LDehy pre: 5.6 ± 3.5, post: 10.4 ± 6.2, P = 0.16). In FDehy, Posm increased following FR (pre: 288 ± 2, post: 292 ± 1, P = 0.03) but not in men (pre: 292 ± 3, post: 293 ± 2, P = 0.46). No MC differences were observed between body mass loss, Posm, Uosm, USG, and copeptin ( P > 0.05). These results suggest that volume-regulatory responses to 24-h FR were present in men but not in women, without apparent effects of the menstrual cycle.

scholarly journals Penentuan Umur Simpan Yoghurt Sinbiotik Dengan Penambahan Tepung Gembolo Modifikasi Fisik

EDUFORTECH ◽  
2017 ◽  
Vol 2 (1) ◽  
Author(s):  
Rizqy Zainul Ihsan ◽  
Dewi Cakrawati ◽  
Mustika Nuramalia Handayani ◽  
Sri Handayani
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Shelf Life ◽  
Room Temperature ◽  
Arrhenius Equation ◽  
Storage Temperature ◽  
Critical Factor ◽  
Acid Value ◽  
Total Acid ◽  
C Storage

The study aim was to determine the shelf life of yogurt sinbiotik at several temperatures with the addition of Dioscorea esculenta modified flour. Lactic Acid Bacteria used for yoghurt making consist of Lactobacillus acidophilus, Bifidobacterium longun, Streptococcus themophillus. The method used Accelerated shelf life test (ASLT) with the characteristics observed namely pH, total acid value using titration method, and total lactic acid bacteria with TPC method. The concentration level of modified flour of Dioscorea esculenta was 2%. The shelf life of yogurt can be determined from the critical factor based on most rapid damage to the Arrhenius equation y = -3504,1x + 9.1043 and R2 = 0.5125. Yogurt sinbiotik stored at room temperature shelf life is 9.5 days. At 4 ° C storage temperature, shelf life is 23.2 days. At 40 ° C storage temperature, shelf life is 5.4 days. The addition of Dioscorea esculenta tuber starch in yogurt sinbiotik affect viability of LAB in the yogurt, since viability of lactic acid bacteria during storage has decreased, but the numbers are still within the range of CFU / ml on the final day of storage 

Impact of Bolus versus Metered Fluid Consumption on Urine Specific Gravity and Urine Color

2008 ◽  
Vol 40 (Supplement) ◽  
pp. S189
Author(s):  
Eric J. Jones ◽  
Phil A. Bishop ◽  
James M. Green ◽  
Mark T. Richardson
Keyword(s):  
Specific Gravity ◽  
Urine Specific Gravity ◽  
Fluid Consumption ◽  
Urine Color

scholarly journals Pengetahuan, sikap, perilaku, dan asupan gizi berdasarkan status hidrasi ibu hamil

2020 ◽  
Vol 17 (2) ◽  
pp. 87
Author(s):  
Erry Yudhya Mulyani ◽  
Idrus Jus'at ◽  
Dudung Angkasa ◽  
Dwikani Oklita Anggiruling ◽  
Enrico Stanin
Keyword(s):  
Specific Gravity ◽  
Pregnant Women ◽  
Nutritional Intake ◽  
Urine Specific Gravity ◽  
Hydration Status ◽  
Nutritional Knowledge ◽  
Cross Sectional ◽  
Urine Color ◽  
And Behavior ◽  
Attitudes And Behavior

Maternal nutritional knowledge, attitude, behavior, and intake based on hydration statusBackground: Based on past studies, 49% of pregnant women had low knowledge of nutrition and hydration. Nutritional knowledge, attitudes, and behavior are positively correlated to nutritional intake, malnutrition and dehydration may disturb maternal health and pregnancy outcome.Objectives: To analyze maternal nutritional knowledge, attitudes, behavior, and nutritional intake based on the hydration status.Methods: This cross-sectional study was conducted at Puskesmas Kebon Jeruk District, West Jakarta using a purposive sampling method. Subjects were 50 pregnant women who came to check their pregnancies. Subject’s characteristics, nutritional knowledge, attitudes, and behavior are collected with a questionnaire, nutritional intake with 1x24 hours recall. Hemoglobin (Hb) level is determined with Haemometer, urine specific gravity with Urinometer, and urine color with PURI card.Results: Subjects had an average age of 29.0±5.7 years, gestational age 21.3±11.3 weeks, weight 62.7±9 kg, height 158.1±4.1 cm, upper arm circumference 32.4±29.5 cm, Hb level 12.2±0.9 g/dL, urine color score 4±0.9, and urine specific gravity 1016±5.4. Based on urine specific gravity, 56% of the pregnant women were euhydrated and 44% were dehydrated. Based on the hydration status there were no differences in knowledge, attitudes, behavior, and macronutrient intake (p≥0.05), except for the fluid intake (p<0.05).Conclusions: Although the knowledge, attitudes, and nutritional behavior of pregnant women were not poor, optimization of the fluid consumption to 3L/day, and diversified food consumption is needed. Further research on external factors that can affect the nutritional status and hydration of pregnant women is recommended.

Relationships Between WUT (Body Weight, Urine Color, and Thirst Level) Criteria and Urine Indices of Hydration Status

2021 ◽  
pp. 194173812110384
Author(s):  
Yasuki Sekiguchi ◽  
Courteney L. Benjamin ◽  
Cody R. Butler ◽  
Margaret C. Morrissey ◽  
Erica M. Filep ◽  
...  
Keyword(s):  
Specific Gravity ◽  
Mass Loss ◽  
Body Mass ◽  
Urine Osmolality ◽  
Urine Specific Gravity ◽  
Venn Diagram ◽  
Hydration Status ◽  
Body Mass Loss ◽  
Living Situation ◽  
Urine Color

Background: A Venn diagram consisting of percentage body mass loss, urine color, and thirst perception (weight, urine, thirst [WUT]) has been suggested as a practical method to assess hydration status. However, no study to date has examined relationships between WUT and urine hydration indices. Thus, the purpose of this study was to investigate relationships between urine specific gravity, urine osmolality, and the WUT criteria. Hypothesis: Urine specific gravity and urine osmolality indicate hypohydration when the WUT criteria demonstrate hypohydration (≥2 markers). Study Design: Laboratory cohort study. Level of Evidence: Level 3. Methods: A total of 22 women (mean ± SD; age, 20 ± 1 years; mass, 65.4 ± 12.6 kg) and 21 men (age, 21 ± 1 years; body mass, 78.7 ± 14.6 kg) participated in this study. First morning body mass, urine color, urine specific gravity, urine osmolality, and thirst level were collected for 10 consecutive days in a free-living situation. Body mass loss >1%, urine color >5, and thirst level ≥5 were used as the dehydration thresholds. The number of markers that indicated dehydration levels were counted and categorized into either 3, 2, 1, or 0 WUT markers that indicated dehydration. One-way analysis of variance with Tukey pairwise comparisons was used to assess the differences in urine specific gravity and urine osmolality between the different number of WUT markers. Results: Urine specific gravity in 3 WUT markers (mean ± SD [effect size], 1.021 ± 0.007 [0.57]; P = 0.025) and 2 WUT markers (1.019 ± 0.010 [0.31]; P = 0.026) was significantly higher than 1 WUT marker (1.016 ± 0.009). Urine mosmolality in 2 WUT markers (705 ± 253 mOsmol [0.43]; P = 0.018) was significantly higher than 1 WUT (597 ± 253 mOsmol). Meeting at least 2 WUT markers resulted in sensitivities of 0.652 (2 WUT criteria met) and 0.933 (3 WUT criteria met) to detect urine osmolality >700 mOsmol. Conclusion: These results suggest that when 3 WUT markers are met, urine specific gravity and urine osmolality were greater than euhydration cutoff points. The WUT criterion is a useful tool to use in field settings to assess hydration status when first morning urine sample was used. Clinical Relevance: Athletes, coaches, sports scientists, and medical professionals can use WUT criteria to monitor dehydration with reduced cost and time.

Use of a Urine Color Chart to Monitor Hydration Status in Nursing Home Residents

2006 ◽  
Vol 7 (3) ◽  
pp. 197-203 ◽  
Author(s):  
Janet C. Mentes ◽  
Bonnie Wakefield ◽  
Kennith Culp
Keyword(s):  
Renal Function ◽  
Nursing Home ◽  
Specific Gravity ◽  
Mixed Model ◽  
Rank Order ◽  
Nursing Home Residents ◽  
Urine Specific Gravity ◽  
Hydration Status ◽  
Color Chart ◽  
Urine Color

To determine whether urine color, as measured by a color chart, might be a valid indicator of hydration status in frail nursing home residents, this study tested the associations between urine color and urine specific gravity. This is a descriptive correlational study set in seven nursing homes in eastern Iowa. Ninety-eight nursing home residents 65 years of age participated. Exclusion criteria for the study included: unstable congestive heart failure or diabetes, documented renal disease, hyponatremia (serum sodium < 135 meq/L), terminal illness, acutely confused/delirious or urinary tract infection at baseline, and gastrostomy-tube dependence. Weekly urine specimens were collected. Ucol was measured first, using a urine color chart. Usg was determined using the Chemstrip Mini UA Urine Analyzer. Week-by-week Spearman rank order correlations between urine color and specific gravity for the total sample (n = 98) ranged from rs= 0.3 - 0.7, p < .01; the PROC mixed model was significant, p < .01. In subgroup analyses (n = 78), all females (rs= 0.67, p = .01) and both males (rs= 0.53, p = .01) and females (rs= 0.72, p = .01) with adequate renal function (Cockcroft-Gault estimated creatinine clearance [CrCl] values of 50 ml/min) had significant associations between average urine color and average Usg. Females with mild renal impairment (CrCl between 30 and 50 ml/min) also had significant associations between Ucol and Usg (rs= .64, p < .01). Ucol averaged over several individual readings offers another tool in assessing hydration status in Caucasian nursing home residents with ade quate renal function measures by estimated CrCl values.

scholarly journals 931 PB 512 COMPARISON OF THREE CULTIVARS AND TWO HYBRIDS OF ONIONS (TEXAS GRANEX 502 PRR, TEXAS GRANEX 502, HOUSTON, HYBRID GRANEX 33, AND HYBRID GRANEX 429) ON DIFFERENT STORAGE TEMPERATURE

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 567e-567
Author(s):  
Juan E. Manzano ◽  
Judith Zambrano ◽  
Douglas Delgado
Keyword(s):  
Weight Loss ◽  
Room Temperature ◽  
Storage Temperature ◽  
Titratable Acidity ◽  
Solid Content ◽  
Soluble Solid Content ◽  
C Storage ◽  
Soluble Solid ◽  
Ph Increase

Aproximately one Kg of Onions bulbs were put in a plastic net and stored at 5°C, 12°C and room temperature (25-3B)°C, with three replications. Analysis of samples were made weekly to detect weight loss during storage for thirteen weeks. Determination of soluble solid content, pH, titratable acidity, reducing sugars were made at weeks two and twelve. Onions stored at room temperature were completely rot, in the week fourteen. Weight loss was lower at 5°C storage In the cultivar Texas Granex 502. In this cultivar soluble solid and pH increase during storage to low temperature while titratable acidity and reducing sugar decreased. The cultivar Texas Granex 502 was the best.

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