Systemic IL-6 and Myoglobin Response to Three Different Resistance Exercise Intensities in Older Men

2018 ◽  
Vol 26 (3) ◽  
pp. 451-456 ◽  
Author(s):  
Stephen M. Cornish ◽  
Jeremie E. Chase ◽  
Eric M. Bugera ◽  
Gordon G. Giesbrecht
Keyword(s):  
Resistance Exercise ◽  
Interleukin 6 ◽  
Older Men ◽  
Blood Samples ◽  
Repetition Maximum ◽  
Time Points ◽  
Baseline Values

The purpose of this research was to identify if three different intensities of resistance exercise would acutely and differentially effect the systemic release of interleukin-6 (IL-6) and myoglobin in older men (≥65 years). A total of 11 older men performed isovolume resistance exercise on six different apparatuses at three different intensities (144 reps at 60%, 120 reps at 72%, and 108 reps at 80% of 1-repetition maximum), with the intensity order randomly allocated, to determine the systemic release of IL-6 and myoglobin in the blood. Blood samples were collected at six time points, including preexercise, immediately postexercise, and 3, 6, 24, and 48 hr postexercise. There were no differences between intensity levels; therefore, data for all conditions were pooled. IL-6 did not show any change from baseline values throughout all time points (p > .05), whereas myoglobin was elevated at 3, 6, and 24 hr postexercise and returned to baseline after 48 hr (p < .05).

LENGTH OF REST INTERVAL BETWEEN RESISTANCE EXERCISE SETS: PERFORMANCE AND INTER-INDIVIDUAL VARIABILITY OF CK ACTIVITY

2010 ◽  
Vol 13 (04) ◽  
pp. 187-195 ◽  
Author(s):  
Lara E. Silva ◽  
Rafael Pereira ◽  
Vanessa C. Rodrigues ◽  
Pierre A. V. Silva ◽  
Kênia M. Silva ◽  
...  
Keyword(s):  
Resistance Exercise ◽  
Exercise Bout ◽  
Elbow Flexion ◽  
Individual Variability ◽  
Rest Interval ◽  
Exercise Protocol ◽  
Blood Samples ◽  
Repetition Maximum ◽  
The One ◽  
The Relationship

Purpose: To determine how the rest interval (RI) lengths of 30 and 90 s between sets of biceps curl exercise affect the total volume of work performed and serum CK activity, and to verify the relationship between inter-individual variability of CK activity and total volume performed when the resistance exercise bout is conducted with 30 or 90 s Methods: Twenty-seven healthy sedentary men (18–20 years old) volunteered to participate in this study and were divided into two groups: 30RI (n = 16) or 90RI (n = 11), based on the RI length of 30 or 90 s between the sets of a resistance exercise protocol. The one repetition maximum (1RM) assessment of the elbow flexion was carried out and then a resistance exercise protocol which constituted five sets of biceps curl at 85% of 1RM with 30-s (30RI group) or 90-s (90RI group) RI length between sets was performed. Each bout was performed to voluntary fatigue and the number of repetitions and workout volume completed were calculated. Subjects provided blood samples prior to resistance exercise, and at 48 and 96h following exercise to evaluate serum CK activity. The inter-individual serum CK activity along the 96h after exercise was analyzed. Results: The results demonstrated that the longer RIs provided greater workout volume as expected, but there were no differences in serum CK activity between the groups. Additionally, it was possible to identify two high responder subjects, one from each RI group, who showed a great inter-individual serum CK activity variability. Conclusion: Exercising with short RIs does not appear to present any additional muscle microtrauma to untrained subjects. Further studies are necessary to evaluate if the inter-individual variability of the serum CK activity is influenced by the inter-set RI length.

Serum leptin responses after acute resistance exercise protocols

2003 ◽  
Vol 94 (2) ◽  
pp. 591-597 ◽  
Author(s):  
A. Zafeiridis ◽  
I. Smilios ◽  
R. V. Considine ◽  
S. P. Tokmakidis
Keyword(s):  
Growth Hormone ◽  
Body Weight ◽  
Resistance Exercise ◽  
Body Fat ◽  
Muscular Hypertrophy ◽  
Acute Effects ◽  
Blood Samples ◽  
Serum Leptin ◽  
Repetition Maximum ◽  
And Control

This study examined the acute effects of maximum strength (MS), muscular hypertrophy (MH), and strength endurance (SE) resistance exercise protocols on serum leptin. Ten young lean men (age = 23 ± 4 yr; body weight = 79.6 ± 5.2 kg; body fat = 10.2 ± 3.9%) participated in MS [4 sets × 5 repetitions (reps) at 88% of 1 repetition maximum (1 RM) with 3 min of rest between sets], MH (4 sets × 10 reps at 75% of 1 RM with 2 min of rest between sets), SE (4 sets × 15 reps at 60% of 1 RM with 1 min of rest between sets), and control (C) sessions. Blood samples were collected before and immediately after exercise and after 30 min of recovery. Serum leptin at 30 min of recovery exhibited similar reductions from baseline after the MS (−20 ± 5%), MH (−20 ± 4%), and SE (−15 ± 6%) protocols that were comparable to fasting-induced reduction in the C session (−12 ± 3%) ( P < 0.05). Furthermore, no differences were found in serum leptin among the MS, MH, SE, and C sessions immediately after exercise and at 30 min of recovery ( P > 0.05). Cortisol was higher ( P < 0.05) after the MH and SE protocols than after the MS and C sessions. Glucose and growth hormone were higher ( P < 0.05) after exercise in the MS, MH, and SE protocols than after the C session. In conclusion, typical resistance exercise protocols designed for development of MS, MH, and SE did not result in serum leptin changes when sampled immediately or 30 min postexercise.

scholarly journals Correlation between L-Lactate Concentrations in Beef Cattle, Obtained Using a Hand-Held Lactate Analyzer and a Lactate Assay Colorimetric Kit

Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 926
Author(s):  
Daniela M. Meléndez ◽  
Sonia Marti ◽  
Luigi Faucitano ◽  
Derek B. Haley ◽  
Timothy D. Schwinghamer ◽  
...  
Keyword(s):  
Statistical Significance ◽  
Road Transportation ◽  
Long Distance ◽  
Suitable Alternative ◽  
Blood Samples ◽  
Time Points ◽  
Pooled Data ◽  
Red Angus ◽  
Relationship Of ◽  
The Relationship

Lactate is a product of anaerobic glycolysis, used in animal research as an indicator of muscle fatigue. Therefore, it has been used as an indicator of cattle response to long distance transportation. The aim of this study was to assess the relationship of L-lactate concentrations measured using a Lactate Scout+ analyzer and a traditional lactate assay colorimetric kit. Blood samples were collected by venipuncture from 96 steers (Black or Red Angus × Hereford/Simmental and Black or Red Angus × Charolais; 247 ± 38.2 kg BW) prior to loading (LO1) and after 36 h of transport, and prior to reloading and after an additional 4 h of road transportation, and on d 1, 2, 3, 5, 14, and 28 after transport. The Lactate Scout+ analyzer strip was dipped in blood at the time of sampling, while blood samples were collected into sodium fluoride tubes for use in the colorimetric analysis. Pearson correlations were calculated to assess the strength of the relationship between the experimental methods for the quantification of L-lactate concentrations. The magnitude and direction of the correlation, and the level of statistical significance varied over the observed time points, ranging from r = −0.03 (p = 0.75; LO1) to r = 0.75 (p < 0.0001; d 3). The correlation for the pooled data was weak but statistically significant (r = 0.33, p < 0.0001). Based on the low magnitude of the correlation due to variability across sampling time points in this study, the Lactate Scout+ analyzer is not a suitable alternative to a lab-based assay (considered the gold standard) for measuring L-lactate in transported cattle.

scholarly journals PSVII-3 Correlations between L-lactate concentrations obtained using a handheld lactate analyser and a lactate assay colorimetric kit in beef cattle transported by road

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 296-297
Author(s):  
Daniela M Meléndez ◽  
Sonia Marti ◽  
Luigi Faucitano ◽  
Derek B Haley ◽  
Timothy D Schwinghamer ◽  
...  
Keyword(s):  
Statistical Significance ◽  
Cost Effective ◽  
Road Transportation ◽  
Long Distance ◽  
Suitable Alternative ◽  
Blood Samples ◽  
Handheld Device ◽  
Time Points ◽  
Relationship Of ◽  
The Relationship

Abstract Blood metabolites are used to assess a variety of animal conditions for veterinary diagnosis and research. Concentration of metabolites in blood can be measured using a commercially-available lab-based assay or in real-time using a handheld device developed to be more time- and cost-effective than the lab-based method. Lactate is a product of anaerobic glycolysis, used in animal research as an indicator of muscle fatigue. Therefore, it has been used as an indicator of cattle response to long distance transportation. The aim of this study was to assess the relationship of L-lactate concentrations measured using a Lactate Scout+ analyzer (Lactate Scout, EFK Diagnostics, Barleben, Germany) and a lactate assay colorimetric kit (Lactate Assay Kit, Cell Biolabs Inc., San Diego, CA). Blood samples were collected by venipuncture from 96 steers (245 ± 35.7 kg BW) prior to (L1) and after 36 h, and prior to and after an additional 4 h of road transportation, and on d 1, 2, 3, 5, 14, and 28 after transport. The Lactate Scout+ analyzer strip was dipped in blood at the time of sampling, while blood samples were collected into sodium fluoride tubes for use in colorimetric analysis. Pearson correlations were calculated to determine the relationship between the experimental methods for the quantification of L-lactate concentrations. The strengths and levels of statistical significance of the correlation varied over the observed time points, r = -0.03, P = 0.75 (L1) to r = 0.75, P = &lt; 0.0001 (d 3). The correlation for the pooled data was weak but statistically significant (r = 0.33, P &lt; 0.001). Based on the experimental results, the Lactate Scout+ analyzer is not a suitable alternative to a lab-based assay for measuring L-lactate in transported cattle, due to variability across sampling time points and weak correlation with the traditional enzymatic method.

Association between serum interleukin-6 and frailty in older men: cross-sectional data

2021 ◽  
Author(s):  
Monica C. Tembo ◽  
Kara L. Holloway-Kew ◽  
Chiara C. Bortolasci ◽  
Sharon L. Brennan-Olsen ◽  
Lana J. Williams ◽  
...  
Keyword(s):  
Interleukin 6 ◽  
Older Men ◽  
Cross Sectional

scholarly journals Recovery-Stress Response of Blood-Based Biomarkers

2021 ◽  
Vol 18 (11) ◽  
pp. 5776
Author(s):  
Sebastian Hacker ◽  
Thomas Reichel ◽  
Anne Hecksteden ◽  
Christopher Weyh ◽  
Kristina Gebhardt ◽  
...  
Keyword(s):  
Calcium Binding ◽  
Tissue Repair ◽  
Elite Athletes ◽  
Venous Blood ◽  
Soccer Players ◽  
Recovery Stress ◽  
Blood Samples ◽  
Time Points ◽  
S100 Calcium Binding Protein ◽  
Stress States

The purpose of this study was to investigate blood-based biomarkers and their regulation with regard to different recovery-stress states. A total of 35 male elite athletes (13 badminton, 22 soccer players) were recruited, and two venous blood samples were taken: one in a ‘recovered’ state (REC) after a minimum of one-day rest from exercise and another one in a ‘non-recovered’ state (NOR) after a habitual loading microcycle. Overall, 23 blood-based biomarkers of different physiologic domains, which address inflammation, muscle damage, and tissue repair, were analyzed by Luminex assays. Across all athletes, only creatine kinase (CK), interleukin (IL-) 6, and IL-17A showed higher concentrations at NOR compared to REC time points. In badminton players, higher levels of CK and IL-17A at NOR were found. In contrast, a higher value for S100 calcium-binding protein A8 (S100A8) at REC was found in badminton players. Similar differences were found for BDNF in soccer players. Soccer players also showed increased levels of CK, and IL-6 at NOR compared to REC state. Several molecular markers were shown to be responsive to differing recovery-stress states, but their suitability as biomarkers in training must be further validated.

scholarly journals Local cooling during hot water immersion improves perceptions without inhibiting the acute interleukin-6 response

2021 ◽  
Author(s):  
R. G. Mansfield ◽  
S. P. Hoekstra ◽  
J. J. Bill ◽  
Christof A. Leicht
Keyword(s):  
Thermal Comfort ◽  
Interleukin 6 ◽  
Water Immersion ◽  
Hot Water ◽  
Upper Body ◽  
Healthy Male ◽  
Local Cooling ◽  
Blood Samples ◽  
Body Cooling ◽  
Hot Water Immersion

Abstract Purpose Passive elevation of body temperature can induce an acute inflammatory response that has been proposed to be beneficial; however, it can be perceived as uncomfortable. Here, we investigate whether local cooling of the upper body during hot water immersion can improve perception without inhibiting the interleukin-6 (IL-6) response. Methods Nine healthy male participants (age: 22 ± 1 years, body mass: 83.4 ± 9.4 kg) were immersed up to the waist for three 60-min water immersion conditions: 42 °C hot water immersion (HWI), 42 °C HWI with simultaneous upper-body cooling using a fan (FAN), and 36 °C thermoneutral water immersion (CON). Blood samples to determine IL-6 plasma concentration were collected pre- and post-water immersion; basic affect and thermal comfort were assessed throughout the intervention. Results Plasma IL-6 concentration was higher for HWI and FAN when compared with CON (P < 0.01) and did not differ between HWI and FAN (P = 0.22; pre to post, HWI: 1.0 ± 0.6 to 1.5 ± 0.7 pg·ml−1, FAN: 0.7 ± 0.5 to 1.1 ± 0.5 pg·ml−1, CON: 0.5 ± 0.2 to 0.5 ± 0.2 pg·ml−1). At the end of immersion, basic affect was lowest for HWI (HWI: − 1.8 ± 2.0, FAN: 0.2 ± 1.6, CON 1.0 ± 2.1, P < 0.02); thermal comfort for HWI was in the uncomfortable range (3.0 ± 1.0, P < 0.01 when compared with FAN and CON), whereas FAN (0.7 ± 0.7) and CON (-0.2 ± 0.7) were in the comfortable range. Conclusion Local cooling of the upper body during hot water immersion improves basic affect and thermal comfort without inhibiting the acute IL-6 response.

Identification of anaerobic threshold by analysis of heart rate variability during discontinuous dynamic and resistance exercise protocols in healthy older men

2013 ◽  
Vol 34 (2) ◽  
pp. 98-108 ◽  
Author(s):  
Rodrigo Polaquini Simões ◽  
Viviane Castello-Simões ◽  
Renata Gonçalves Mendes ◽  
Bruno Archiza ◽  
Daniel Augusto dos Santos ◽  
...  
Keyword(s):  
Heart Rate ◽  
Heart Rate Variability ◽  
Resistance Exercise ◽  
Anaerobic Threshold ◽  
Older Men

Acute Blood Pressure Response to High- and Moderate-Speed Resistance Exercise in Older Adults With Hypertension

2021 ◽  
pp. 1-8
Author(s):  
Carlos Leonardo Figueiredo Machado ◽  
Régis Radaelli ◽  
Clarissa Muller Brusco ◽  
Eduardo Lusa Cadore ◽  
Eurico N. Wilhelm ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Older Adults ◽  
Resistance Exercise ◽  
Blood Pressure Response ◽  
Individual Response ◽  
Older Individuals ◽  
Repetition Maximum ◽  
Control Protocol ◽  
The Individual ◽  
And Control

An increase in blood pressure (BP) occurs during resistance exercise; attention to this response may be necessary in older individuals with hypertension. We compared the BP responses following high- (HSRE) and moderate-speed resistance exercise (MSRE) (4 × 8 repetitions at 60% one-repetition maximum) and control protocol in 15 older adults with hypertension. HSRE and MSRE increased systolic BP (SBP) by the end of each set compared with preexercise and control protocol. Immediately after the fourth set, a higher SBP was observed in MSRE than HSRE (147 ± 14 vs. 141 ± 12 mmHg; p = .01). Taking an exploratory analysis of the individual response, we observed that MSRE resulted in greater mean changes and number of SBP exposures to values ≥150 mmHg (22-fold) than HSRE (10-fold). Diastolic BP increased (p < .05) with exercise, but only MSRE increased compared with the control condition (p < .05). HSRE may be an alternative for individuals in which SBP peak should be avoided.

scholarly journals Creatine Kinase and Lactate Dehydrogenase Responses After Different Resistance and Aerobic Exercise Protocols

2017 ◽  
Vol 58 (1) ◽  
pp. 65-72 ◽  
Author(s):  
Gustavo A. Callegari ◽  
Jefferson S. Novaes ◽  
Gabriel R. Neto ◽  
Ingrid Dias ◽  
Nuno D. Garrido ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Lactate Dehydrogenase ◽  
Resistance Exercise ◽  
Aerobic Exercise ◽  
Aerobic Training ◽  
Body Height ◽  
Blood Samples ◽  
Set Protocol ◽  
Experimental Protocols ◽  
Elevate Plasma

AbstractThe aim of this study was to investigate the responses of creatine kinase (CK) and lactate dehydrogenase (LDH) after performing different resistance and aerobic exercise protocols. Twelve recreationally trained men (age, 23.2 ± 5.6 years; body mass, 84.3 ± 9.3 kg; body height, 178.9 ± 4.5 cm; and BMI, 26.3 ± 2.3 kg·m2) volunteered to participate in this study. All subjects were randomly assigned to four experimental protocols (crossover): (a) aerobic training at 60% of VO2max, (b) aerobic training at 80% of VO2max, (c) a resistance exercise (RE) session with a bi-set protocol, and (d) an RE session with a multiple sets protocol. Blood samples were collected before, immediately after and 24 hours following the experimental protocols. After 24 hours, there was a significant increase in CK for the 80% of VO2max protocol vs. the bi-set RE session (p = 0.016). Immediately after the protocols, we observed a significant increase in LDH among certain groups compared to others, as follows: multiple sets RE session vs. 60% of VO2max, bi-set RE session vs. 60% of VO2max, multiple sets RE session vs. 80% of VO2max, and bi-set RE session vs. 80% of VO2max (p = 0.008, p = 0.013; p = 0.002, p = 0.004, respectively). In conclusion, aerobic exercise performed at 80% of VO2max appears to elevate plasma CK levels more than bi-set RE sessions. However, the bi-set and multiple sets RE sessions appeared to trigger greater levels of blood LDH compared to aerobic protocols performed at 60% and 80% of VO2max.

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