Staphylococcus aureus Cell Extract Transcription-Translation Assay: Firefly Luciferase Reporter System for Evaluating Protein Translation Inhibitors
2001 ◽
Vol 45
(6)
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pp. 1900-1904
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Keyword(s):
ABSTRACT The promoter for the Staphylococcus aureus capsule polysaccharide synthesis gene (cap1A) was cloned in front of the firefly luciferase gene for use in a cell extractS. aureus transcription-translation system. The assay is rapid, reproducible, and sensitive and has a lower background level than the radiolabeled amino acid incorporation translation assays. We present data evaluating a transcription inhibitor and a number of protein translation inhibitors in this system.
2012 ◽
Vol 18
(4)
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pp. 453-461
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Keyword(s):
2001 ◽
Vol 45
(12)
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pp. 3456-3461
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Keyword(s):
2013 ◽
Vol 58
(3)
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pp. 1389-1396
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2005 ◽
Vol 280
(43)
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pp. 36055-36064
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Keyword(s):
2010 ◽
Vol 37
(11)
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pp. 2268-2272
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